This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 95.33 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 28,473 (0.9%) Reads that were too short: 10,476 (0.3%) Reads written (passing filters): 17,997 (0.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 3,040,801 bp (0.3%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 28473 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 72 779.6 0 72 7 17 779.6 0 17 8 10 779.6 0 10 9 12 779.6 0 12 10 7 779.6 0 7 11 11 779.6 0 11 12 7 779.6 0 7 13 17 779.6 0 17 14 10 779.6 0 10 15 7 779.6 0 7 16 13 779.6 0 13 17 26 779.6 0 26 18 20 779.6 0 20 19 7 779.6 0 7 20 7 779.6 0 7 21 4 779.6 0 4 22 9 779.6 0 9 23 6 779.6 0 6 24 5 779.6 0 5 25 7 779.6 0 7 26 8 779.6 0 8 27 6 779.6 0 6 28 7 779.6 0 7 29 9 779.6 0 9 30 14 779.6 0 14 31 10 779.6 0 10 32 9 779.6 0 9 33 16 779.6 0 16 34 8 779.6 0 8 35 7 779.6 0 7 36 13 779.6 0 13 37 9 779.6 0 9 38 15 779.6 0 15 39 15 779.6 0 15 40 15 779.6 0 15 41 19 779.6 0 19 42 14 779.6 0 14 43 11 779.6 0 11 44 93 779.6 0 93 45 171 779.6 0 171 46 54 779.6 0 54 47 10 779.6 0 10 48 8 779.6 0 8 49 11 779.6 0 11 50 5 779.6 0 5 51 41 779.6 0 41 52 15 779.6 0 15 53 111 779.6 0 111 54 18 779.6 0 18 55 4 779.6 0 4 56 41 779.6 0 41 57 47 779.6 0 47 58 91 779.6 0 91 59 51 779.6 0 51 60 32 779.6 0 32 61 11 779.6 0 11 62 5 779.6 0 5 63 8 779.6 0 8 64 10 779.6 0 10 65 3 779.6 0 3 66 8 779.6 0 8 67 5 779.6 0 5 68 13 779.6 0 13 69 9 779.6 0 9 70 4 779.6 0 4 71 5 779.6 0 5 72 11 779.6 0 11 73 9 779.6 0 9 74 32 779.6 0 32 75 67 779.6 0 67 76 9 779.6 0 9 77 9 779.6 0 9 78 5 779.6 0 5 79 12 779.6 0 12 80 17 779.6 0 17 81 34 779.6 0 34 82 35 779.6 0 35 83 46 779.6 0 46 84 25 779.6 0 25 85 19 779.6 0 19 86 20 779.6 0 20 87 18 779.6 0 18 88 21 779.6 0 21 89 11 779.6 0 11 90 29 779.6 0 29 91 49 779.6 0 49 92 41 779.6 0 41 93 39 779.6 0 39 94 34 779.6 0 34 95 23 779.6 0 23 96 26 779.6 0 26 97 81 779.6 0 81 98 44 779.6 0 44 99 32 779.6 0 32 100 17 779.6 0 17 101 33 779.6 0 33 102 13 779.6 0 13 103 26 779.6 0 26 104 66 779.6 0 66 105 19 779.6 0 19 106 189 779.6 0 189 107 695 779.6 0 695 108 704 779.6 0 704 109 24 779.6 0 24 110 29 779.6 0 29 111 21 779.6 0 21 112 26 779.6 0 26 113 46 779.6 0 46 114 334 779.6 0 334 115 225 779.6 0 225 116 1125 779.6 0 1125 117 16 779.6 0 16 118 20 779.6 0 20 119 38 779.6 0 38 120 21 779.6 0 21 121 34 779.6 0 34 122 46 779.6 0 46 123 28 779.6 0 28 124 61 779.6 0 61 125 57 779.6 0 57 126 42 779.6 0 42 127 43 779.6 0 43 128 110 779.6 0 110 129 72 779.6 0 72 130 79 779.6 0 79 131 36 779.6 0 36 132 33 779.6 0 33 133 84 779.6 0 84 134 167 779.6 0 167 135 225 779.6 0 225 136 116 779.6 0 116 137 97 779.6 0 97 138 128 779.6 0 128 139 88 779.6 0 88 140 314 779.6 0 314 141 424 779.6 0 424 142 807 779.6 0 807 143 138 779.6 0 138 144 67 779.6 0 67 145 71 779.6 0 71 146 89 779.6 0 89 147 160 779.6 0 160 148 1694 779.6 0 1694 149 2466 779.6 0 2466 150 1408 779.6 0 1408 151 27 779.6 0 27 152 28 779.6 0 28 153 19 779.6 0 19 154 37 779.6 0 37 155 236 779.6 0 236 156 80 779.6 0 80 157 106 779.6 0 106 158 93 779.6 0 93 159 25 779.6 0 25 160 55 779.6 0 55 161 49 779.6 0 49 162 94 779.6 0 94 163 187 779.6 0 187 164 124 779.6 0 124 165 173 779.6 0 173 166 71 779.6 0 71 167 76 779.6 0 76 168 41 779.6 0 41 169 30 779.6 0 30 170 17 779.6 0 17 171 41 779.6 0 41 172 25 779.6 0 25 173 26 779.6 0 26 174 31 779.6 0 31 175 40 779.6 0 40 176 27 779.6 0 27 177 23 779.6 0 23 178 26 779.6 0 26 179 24 779.6 0 24 180 95 779.6 0 95 181 128 779.6 0 128 182 152 779.6 0 152 183 72 779.6 0 72 184 66 779.6 0 66 185 67 779.6 0 67 186 46 779.6 0 46 187 18 779.6 0 18 188 34 779.6 0 34 189 39 779.6 0 39 190 17 779.6 0 17 191 36 779.6 0 36 192 30 779.6 0 30 193 51 779.6 0 51 194 25 779.6 0 25 195 48 779.6 0 48 196 41 779.6 0 41 197 15 779.6 0 15 198 19 779.6 0 19 199 14 779.6 0 14 200 59 779.6 0 59 201 62 779.6 0 62 202 40 779.6 0 40 203 49 779.6 0 49 204 110 779.6 0 110 205 52 779.6 0 52 206 48 779.6 0 48 207 51 779.6 0 51 208 40 779.6 0 40 209 43 779.6 0 43 210 67 779.6 0 67 211 31 779.6 0 31 212 62 779.6 0 62 213 32 779.6 0 32 214 49 779.6 0 49 215 77 779.6 0 77 216 72 779.6 0 72 217 52 779.6 0 52 218 45 779.6 0 45 219 31 779.6 0 31 220 53 779.6 0 53 221 69 779.6 0 69 222 60 779.6 0 60 223 16 779.6 0 16 224 65 779.6 0 65 225 63 779.6 0 63 226 118 779.6 0 118 227 101 779.6 0 101 228 66 779.6 0 66 229 52 779.6 0 52 230 19 779.6 0 19 231 50 779.6 0 50 232 117 779.6 0 117 233 82 779.6 0 82 234 40 779.6 0 40 235 56 779.6 0 56 236 52 779.6 0 52 237 47 779.6 0 47 238 22 779.6 0 22 239 23 779.6 0 23 240 24 779.6 0 24 241 24 779.6 0 24 242 30 779.6 0 30 243 17 779.6 0 17 244 41 779.6 0 41 245 61 779.6 0 61 246 85 779.6 0 85 247 63 779.6 0 63 248 43 779.6 0 43 249 88 779.6 0 88 250 43 779.6 0 43 251 28 779.6 0 28 252 20 779.6 0 20 253 41 779.6 0 41 254 114 779.6 0 114 255 140 779.6 0 140 256 131 779.6 0 131 257 71 779.6 0 71 258 113 779.6 0 113 259 51 779.6 0 51 260 43 779.6 0 43 261 27 779.6 0 27 262 23 779.6 0 23 263 31 779.6 0 31 264 26 779.6 0 26 265 48 779.6 0 48 266 88 779.6 0 88 267 53 779.6 0 53 268 135 779.6 0 135 269 356 779.6 0 356 270 194 779.6 0 194 271 174 779.6 0 174 272 214 779.6 0 214 273 149 779.6 0 149 274 67 779.6 0 67 275 58 779.6 0 58 276 58 779.6 0 58 277 55 779.6 0 55 278 54 779.6 0 54 279 57 779.6 0 57 280 107 779.6 0 107 281 105 779.6 0 105 282 85 779.6 0 85 283 56 779.6 0 56 284 70 779.6 0 70 285 38 779.6 0 38 286 75 779.6 0 75 287 68 779.6 0 68 288 80 779.6 0 80 289 71 779.6 0 71 290 86 779.6 0 86 291 66 779.6 0 66 292 69 779.6 0 69 293 142 779.6 0 142 294 306 779.6 0 306 295 1017 779.6 0 1017 296 1358 779.6 0 1358 297 1037 779.6 0 1037 298 321 779.6 0 321 299 37 779.6 0 37 300 19 779.6 0 19 301 3 779.6 0 3 Finished in 0.68 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 17,997 Reads with adapters: 198 (1.1%) Reads that were too short: 36 (0.2%) Reads written (passing filters): 162 (0.9%) Total basepairs processed: 3,040,801 bp Total written (filtered): 25,177 bp (0.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 198 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 5 18 3 0.0 3 2 0 0 1 19 31 0.0 3 24 5 2 20 1 0.0 3 0 1 21 1 0.0 3 0 0 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 44 0.0 3 0 0 0 44 46 22 0.0 3 0 0 0 22 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 69 10 0.0 3 0 0 0 10 70 28 0.0 3 0 0 0 28 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 113 6 0.0 3 0 0 0 6 114 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 145 17 0.0 3 0 0 2 15 146 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 182 3 0.0 3 0 0 0 3 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 162 Reads with adapters: 4 (2.5%) Reads written (passing filters): 162 (100.0%) Total basepairs processed: 25,177 bp Total written (filtered): 25,164 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 25.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 2.5 0 3 4 1 0.6 0 1