This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.98 s (29 us/read; 2.06 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 40,279 (1.3%) Reads that were too short: 31,231 (1.0%) Reads written (passing filters): 9,048 (0.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 1,726,192 bp (0.2%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 40279 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 24 779.6 0 24 7 26 779.6 0 26 8 20 779.6 0 20 9 155 779.6 0 155 10 11 779.6 0 11 11 29 779.6 0 29 12 16 779.6 0 16 13 11 779.6 0 11 14 11 779.6 0 11 15 10 779.6 0 10 16 635 779.6 0 635 17 16 779.6 0 16 18 21 779.6 0 21 19 9 779.6 0 9 20 12 779.6 0 12 21 10 779.6 0 10 22 14 779.6 0 14 23 6 779.6 0 6 24 10 779.6 0 10 25 14 779.6 0 14 26 11 779.6 0 11 27 7 779.6 0 7 28 5 779.6 0 5 29 11 779.6 0 11 30 9 779.6 0 9 31 9 779.6 0 9 32 14 779.6 0 14 33 11 779.6 0 11 34 13 779.6 0 13 35 9 779.6 0 9 36 10 779.6 0 10 37 10 779.6 0 10 38 13 779.6 0 13 39 16 779.6 0 16 40 9 779.6 0 9 41 11 779.6 0 11 42 11 779.6 0 11 43 12 779.6 0 12 44 12 779.6 0 12 45 12 779.6 0 12 46 4 779.6 0 4 47 9 779.6 0 9 48 8 779.6 0 8 49 14 779.6 0 14 50 251 779.6 0 251 51 20 779.6 0 20 52 11 779.6 0 11 53 16 779.6 0 16 54 22 779.6 0 22 55 13 779.6 0 13 56 15 779.6 0 15 57 10 779.6 0 10 58 16 779.6 0 16 59 10 779.6 0 10 60 18 779.6 0 18 61 24 779.6 0 24 62 14 779.6 0 14 63 26 779.6 0 26 64 11 779.6 0 11 65 18 779.6 0 18 66 33 779.6 0 33 67 444 779.6 0 444 68 467 779.6 0 467 69 596 779.6 0 596 70 23 779.6 0 23 71 150 779.6 0 150 72 7 779.6 0 7 73 21 779.6 0 21 74 25 779.6 0 25 75 32 779.6 0 32 76 13 779.6 0 13 77 6 779.6 0 6 78 7 779.6 0 7 79 11 779.6 0 11 80 16 779.6 0 16 81 24 779.6 0 24 82 12 779.6 0 12 83 27 779.6 0 27 84 41 779.6 0 41 85 44 779.6 0 44 86 38 779.6 0 38 87 40 779.6 0 40 88 47 779.6 0 47 89 15 779.6 0 15 90 13 779.6 0 13 91 24 779.6 0 24 92 68 779.6 0 68 93 28 779.6 0 28 94 28 779.6 0 28 95 28 779.6 0 28 96 23 779.6 0 23 97 17 779.6 0 17 98 79 779.6 0 79 99 121 779.6 0 121 100 15 779.6 0 15 101 4 779.6 0 4 102 8 779.6 0 8 103 5 779.6 0 5 104 9 779.6 0 9 105 84 779.6 0 84 106 6 779.6 0 6 107 3 779.6 0 3 108 45 779.6 0 45 109 27 779.6 0 27 110 10 779.6 0 10 111 5 779.6 0 5 112 5 779.6 0 5 113 10 779.6 0 10 114 14 779.6 0 14 115 20 779.6 0 20 116 20 779.6 0 20 117 15 779.6 0 15 118 27 779.6 0 27 119 36 779.6 0 36 120 26 779.6 0 26 121 69 779.6 0 69 122 21 779.6 0 21 123 16 779.6 0 16 124 23 779.6 0 23 125 18 779.6 0 18 126 20 779.6 0 20 127 20 779.6 0 20 128 16 779.6 0 16 129 24 779.6 0 24 130 85 779.6 0 85 131 72 779.6 0 72 132 256 779.6 0 256 133 120 779.6 0 120 134 207 779.6 0 207 135 17 779.6 0 17 136 26 779.6 0 26 137 12 779.6 0 12 138 14 779.6 0 14 139 16 779.6 0 16 140 12 779.6 0 12 141 7 779.6 0 7 142 9 779.6 0 9 143 20 779.6 0 20 144 19 779.6 0 19 145 21 779.6 0 21 146 12 779.6 0 12 147 25 779.6 0 25 148 29 779.6 0 29 149 19 779.6 0 19 150 14 779.6 0 14 151 19 779.6 0 19 152 49 779.6 0 49 153 66 779.6 0 66 154 102 779.6 0 102 155 58 779.6 0 58 156 25 779.6 0 25 157 31 779.6 0 31 158 145 779.6 0 145 159 44 779.6 0 44 160 44 779.6 0 44 161 34 779.6 0 34 162 22 779.6 0 22 163 56 779.6 0 56 164 68 779.6 0 68 165 56 779.6 0 56 166 54 779.6 0 54 167 35 779.6 0 35 168 31 779.6 0 31 169 22 779.6 0 22 170 16 779.6 0 16 171 22 779.6 0 22 172 66 779.6 0 66 173 25 779.6 0 25 174 25 779.6 0 25 175 35 779.6 0 35 176 24 779.6 0 24 177 32 779.6 0 32 178 87 779.6 0 87 179 53 779.6 0 53 180 62 779.6 0 62 181 56 779.6 0 56 182 65 779.6 0 65 183 123 779.6 0 123 184 155 779.6 0 155 185 175 779.6 0 175 186 69 779.6 0 69 187 18 779.6 0 18 188 28 779.6 0 28 189 50 779.6 0 50 190 45 779.6 0 45 191 27 779.6 0 27 192 92 779.6 0 92 193 101 779.6 0 101 194 83 779.6 0 83 195 29 779.6 0 29 196 80 779.6 0 80 197 53 779.6 0 53 198 36 779.6 0 36 199 77 779.6 0 77 200 96 779.6 0 96 201 176 779.6 0 176 202 127 779.6 0 127 203 36 779.6 0 36 204 75 779.6 0 75 205 39 779.6 0 39 206 36 779.6 0 36 207 44 779.6 0 44 208 77 779.6 0 77 209 101 779.6 0 101 210 56 779.6 0 56 211 31 779.6 0 31 212 59 779.6 0 59 213 57 779.6 0 57 214 93 779.6 0 93 215 134 779.6 0 134 216 161 779.6 0 161 217 268 779.6 0 268 218 386 779.6 0 386 219 6209 779.6 0 6209 220 4388 779.6 0 4388 221 7428 779.6 0 7428 222 230 779.6 0 230 223 30 779.6 0 30 224 30 779.6 0 30 225 37 779.6 0 37 226 42 779.6 0 42 227 46 779.6 0 46 228 25 779.6 0 25 229 30 779.6 0 30 230 16 779.6 0 16 231 16 779.6 0 16 232 43 779.6 0 43 233 71 779.6 0 71 234 58 779.6 0 58 235 30 779.6 0 30 236 54 779.6 0 54 237 63 779.6 0 63 238 75 779.6 0 75 239 114 779.6 0 114 240 85 779.6 0 85 241 75 779.6 0 75 242 61 779.6 0 61 243 34 779.6 0 34 244 43 779.6 0 43 245 49 779.6 0 49 246 39 779.6 0 39 247 60 779.6 0 60 248 314 779.6 0 314 249 1718 779.6 0 1718 250 2443 779.6 0 2443 251 1980 779.6 0 1980 252 93 779.6 0 93 253 62 779.6 0 62 254 98 779.6 0 98 255 60 779.6 0 60 256 64 779.6 0 64 257 66 779.6 0 66 258 55 779.6 0 55 259 67 779.6 0 67 260 40 779.6 0 40 261 41 779.6 0 41 262 50 779.6 0 50 263 29 779.6 0 29 264 67 779.6 0 67 265 46 779.6 0 46 266 27 779.6 0 27 267 23 779.6 0 23 268 25 779.6 0 25 269 75 779.6 0 75 270 77 779.6 0 77 271 55 779.6 0 55 272 50 779.6 0 50 273 50 779.6 0 50 274 55 779.6 0 55 275 33 779.6 0 33 276 39 779.6 0 39 277 73 779.6 0 73 278 47 779.6 0 47 279 200 779.6 0 200 280 318 779.6 0 318 281 243 779.6 0 243 282 112 779.6 0 112 283 57 779.6 0 57 284 95 779.6 0 95 285 106 779.6 0 106 286 45 779.6 0 45 287 52 779.6 0 52 288 34 779.6 0 34 289 43 779.6 0 43 290 71 779.6 0 71 291 94 779.6 0 94 292 72 779.6 0 72 293 45 779.6 0 45 294 46 779.6 0 46 295 56 779.6 0 56 296 50 779.6 0 50 297 111 779.6 0 111 298 94 779.6 0 94 299 40 779.6 0 40 300 23 779.6 0 23 301 41 779.6 0 41 Finished in 0.37 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 9,048 Reads with adapters: 84 (0.9%) Reads that were too short: 16 (0.2%) Reads written (passing filters): 68 (0.8%) Total basepairs processed: 1,726,192 bp Total written (filtered): 10,158 bp (0.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 84 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 1 17 5 0.0 3 5 18 1 0.0 3 0 0 1 19 3 0.0 3 1 0 0 2 20 2 0.0 3 2 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 0 4 105 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 24 0.0 3 0 0 0 24 119 4 0.0 3 0 0 0 4 120 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 122 7 0.0 3 0 0 0 7 123 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 1 161 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 223 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (147 us/read; 0.41 M reads/minute). === Summary === Total reads processed: 68 Reads with adapters: 2 (2.9%) Reads written (passing filters): 68 (100.0%) Total basepairs processed: 10,158 bp Total written (filtered): 10,151 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1.1 0 1 4 1 0.3 0 1