This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 94.95 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 411,621 (12.9%) Reads that were too short: 3,169 (0.1%) Reads written (passing filters): 408,452 (12.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 105,896,085 bp (11.0%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 411621 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1117 779.6 0 1117 7 52787 779.6 0 52787 8 3229 779.6 0 3229 9 156 779.6 0 156 10 260 779.6 0 260 11 130 779.6 0 130 12 121 779.6 0 121 13 362 779.6 0 362 14 365 779.6 0 365 15 320 779.6 0 320 16 277 779.6 0 277 17 438 779.6 0 438 18 517 779.6 0 517 19 913 779.6 0 913 20 14604 779.6 0 14604 21 26143 779.6 0 26143 22 36041 779.6 0 36041 23 232 779.6 0 232 24 145 779.6 0 145 25 290 779.6 0 290 26 89 779.6 0 89 27 91 779.6 0 91 28 49 779.6 0 49 29 94 779.6 0 94 30 93 779.6 0 93 31 175 779.6 0 175 32 265 779.6 0 265 33 475 779.6 0 475 34 460 779.6 0 460 35 986 779.6 0 986 36 2213 779.6 0 2213 37 484 779.6 0 484 38 748 779.6 0 748 39 1372 779.6 0 1372 40 708 779.6 0 708 41 571 779.6 0 571 42 1064 779.6 0 1064 43 968 779.6 0 968 44 1156 779.6 0 1156 45 1088 779.6 0 1088 46 1484 779.6 0 1484 47 1906 779.6 0 1906 48 3369 779.6 0 3369 49 55872 779.6 0 55872 50 83664 779.6 0 83664 51 95545 779.6 0 95545 52 430 779.6 0 430 53 34 779.6 0 34 54 17 779.6 0 17 55 14 779.6 0 14 56 25 779.6 0 25 57 65 779.6 0 65 58 14 779.6 0 14 59 85 779.6 0 85 60 19 779.6 0 19 61 18 779.6 0 18 62 192 779.6 0 192 63 134 779.6 0 134 64 2413 779.6 0 2413 65 109 779.6 0 109 66 6 779.6 0 6 67 10 779.6 0 10 68 6 779.6 0 6 69 9 779.6 0 9 70 17 779.6 0 17 71 9 779.6 0 9 72 9 779.6 0 9 73 8 779.6 0 8 74 6 779.6 0 6 75 8 779.6 0 8 76 5 779.6 0 5 77 11 779.6 0 11 78 12 779.6 0 12 79 12 779.6 0 12 80 15 779.6 0 15 81 8 779.6 0 8 82 9 779.6 0 9 83 14 779.6 0 14 84 16 779.6 0 16 85 21 779.6 0 21 86 28 779.6 0 28 87 10 779.6 0 10 88 16 779.6 0 16 89 25 779.6 0 25 90 7 779.6 0 7 91 5 779.6 0 5 92 8 779.6 0 8 93 9 779.6 0 9 94 12 779.6 0 12 95 9 779.6 0 9 96 3 779.6 0 3 97 7 779.6 0 7 98 14 779.6 0 14 99 7 779.6 0 7 100 7 779.6 0 7 101 4 779.6 0 4 102 15 779.6 0 15 103 7 779.6 0 7 104 12 779.6 0 12 105 8 779.6 0 8 106 8 779.6 0 8 107 6 779.6 0 6 108 7 779.6 0 7 109 4 779.6 0 4 110 11 779.6 0 11 111 5 779.6 0 5 112 11 779.6 0 11 113 3 779.6 0 3 114 7 779.6 0 7 115 4 779.6 0 4 116 13 779.6 0 13 117 31 779.6 0 31 118 62 779.6 0 62 119 47 779.6 0 47 120 15 779.6 0 15 121 13 779.6 0 13 122 18 779.6 0 18 123 11 779.6 0 11 124 90 779.6 0 90 125 48 779.6 0 48 126 26 779.6 0 26 127 11 779.6 0 11 128 36 779.6 0 36 129 12 779.6 0 12 130 27 779.6 0 27 131 64 779.6 0 64 132 14 779.6 0 14 133 7 779.6 0 7 134 10 779.6 0 10 135 9 779.6 0 9 136 5 779.6 0 5 137 5 779.6 0 5 138 8 779.6 0 8 139 13 779.6 0 13 140 13 779.6 0 13 141 11 779.6 0 11 142 7 779.6 0 7 143 9 779.6 0 9 144 11 779.6 0 11 145 10 779.6 0 10 146 9 779.6 0 9 147 10 779.6 0 10 148 9 779.6 0 9 149 8 779.6 0 8 150 8 779.6 0 8 151 6 779.6 0 6 152 7 779.6 0 7 153 5 779.6 0 5 154 6 779.6 0 6 155 6 779.6 0 6 156 14 779.6 0 14 157 14 779.6 0 14 158 27 779.6 0 27 159 41 779.6 0 41 160 7 779.6 0 7 161 9 779.6 0 9 162 11 779.6 0 11 163 7 779.6 0 7 164 9 779.6 0 9 165 10 779.6 0 10 166 6 779.6 0 6 167 8 779.6 0 8 168 10 779.6 0 10 169 12 779.6 0 12 170 13 779.6 0 13 171 10 779.6 0 10 172 12 779.6 0 12 173 13 779.6 0 13 174 6 779.6 0 6 175 9 779.6 0 9 176 15 779.6 0 15 177 27 779.6 0 27 178 31 779.6 0 31 179 33 779.6 0 33 180 70 779.6 0 70 181 62 779.6 0 62 182 467 779.6 0 467 183 686 779.6 0 686 184 544 779.6 0 544 185 37 779.6 0 37 186 45 779.6 0 45 187 45 779.6 0 45 188 46 779.6 0 46 189 43 779.6 0 43 190 43 779.6 0 43 191 74 779.6 0 74 192 147 779.6 0 147 193 2348 779.6 0 2348 194 1599 779.6 0 1599 195 2424 779.6 0 2424 196 928 779.6 0 928 197 191 779.6 0 191 198 42 779.6 0 42 199 14 779.6 0 14 200 10 779.6 0 10 201 8 779.6 0 8 202 5 779.6 0 5 203 5 779.6 0 5 204 6 779.6 0 6 205 15 779.6 0 15 206 47 779.6 0 47 207 12 779.6 0 12 208 15 779.6 0 15 209 15 779.6 0 15 210 13 779.6 0 13 211 7 779.6 0 7 212 16 779.6 0 16 213 144 779.6 0 144 214 64 779.6 0 64 215 102 779.6 0 102 216 69 779.6 0 69 217 20 779.6 0 20 218 21 779.6 0 21 219 47 779.6 0 47 220 25 779.6 0 25 221 41 779.6 0 41 222 31 779.6 0 31 223 18 779.6 0 18 224 6 779.6 0 6 225 18 779.6 0 18 226 20 779.6 0 20 227 65 779.6 0 65 228 6 779.6 0 6 229 12 779.6 0 12 230 14 779.6 0 14 231 10 779.6 0 10 232 12 779.6 0 12 233 8 779.6 0 8 234 31 779.6 0 31 235 11 779.6 0 11 236 34 779.6 0 34 237 23 779.6 0 23 238 19 779.6 0 19 239 41 779.6 0 41 240 155 779.6 0 155 241 127 779.6 0 127 242 38 779.6 0 38 243 11 779.6 0 11 244 8 779.6 0 8 245 17 779.6 0 17 246 24 779.6 0 24 247 40 779.6 0 40 248 17 779.6 0 17 249 18 779.6 0 18 250 116 779.6 0 116 251 136 779.6 0 136 252 78 779.6 0 78 253 42 779.6 0 42 254 12 779.6 0 12 255 9 779.6 0 9 256 17 779.6 0 17 257 33 779.6 0 33 258 16 779.6 0 16 259 13 779.6 0 13 260 6 779.6 0 6 261 10 779.6 0 10 262 7 779.6 0 7 263 34 779.6 0 34 264 38 779.6 0 38 265 81 779.6 0 81 266 59 779.6 0 59 267 23 779.6 0 23 268 12 779.6 0 12 269 10 779.6 0 10 270 9 779.6 0 9 271 8 779.6 0 8 272 8 779.6 0 8 273 7 779.6 0 7 274 13 779.6 0 13 275 15 779.6 0 15 276 8 779.6 0 8 277 11 779.6 0 11 278 8 779.6 0 8 279 9 779.6 0 9 280 7 779.6 0 7 281 9 779.6 0 9 282 12 779.6 0 12 283 21 779.6 0 21 284 23 779.6 0 23 285 23 779.6 0 23 286 49 779.6 0 49 287 28 779.6 0 28 288 57 779.6 0 57 289 173 779.6 0 173 290 99 779.6 0 99 291 17 779.6 0 17 292 9 779.6 0 9 293 20 779.6 0 20 294 102 779.6 0 102 295 20 779.6 0 20 296 7 779.6 0 7 297 9 779.6 0 9 298 5 779.6 0 5 299 6 779.6 0 6 300 59 779.6 0 59 301 43 779.6 0 43 Finished in 22.50 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 408,452 Reads with adapters: 5,895 (1.4%) Reads that were too short: 37 (0.0%) Reads written (passing filters): 5,858 (1.4%) Total basepairs processed: 105,896,085 bp Total written (filtered): 1,318,639 bp (1.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5895 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 41 0.0 3 0 37 1 0 3 20 3166 0.0 4 3131 34 0 1 21 9 0.0 4 3 6 22 2 0.0 4 0 0 1 1 32 1 0.0 4 1 50 4 0.0 4 0 0 0 0 4 61 1 0.0 4 1 62 2 0.0 4 2 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 951 0.0 4 0 0 0 0 951 91 1 0.0 4 0 0 0 0 1 97 4 0.0 4 0 0 0 0 4 110 4 0.0 4 0 0 0 0 4 111 6 0.0 4 0 0 0 0 6 112 1 0.0 4 0 1 113 2 0.0 4 0 0 0 0 2 114 317 0.0 4 0 0 0 0 317 115 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 13 0.0 4 0 0 0 0 13 127 1286 0.0 4 0 0 0 0 1286 128 4 0.0 4 1 0 0 0 3 138 1 0.0 4 0 0 0 0 1 139 4 0.0 4 0 0 0 0 4 140 26 0.0 4 0 0 0 0 26 153 2 0.0 4 0 0 0 0 2 186 4 0.0 4 0 0 0 0 4 191 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 3 0.0 4 0 0 0 0 3 218 4 0.0 4 0 0 0 1 3 219 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 240 2 0.0 4 0 0 0 0 2 241 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 245 6 0.0 4 0 0 0 0 6 249 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 5,858 Reads with adapters: 2,029 (34.6%) Reads written (passing filters): 5,858 (100.0%) Total basepairs processed: 1,318,639 bp Total written (filtered): 776,231 bp (58.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2029 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 0.0% G: 1.4% T: 0.1% none/other: 97.7% Overview of removed sequences length count expect max.err error counts 3 5 91.5 0 5 4 11 22.9 0 11 10 2 0.0 1 0 2 11 26 0.0 1 0 26 260 1 0.0 1 1 268 1 0.0 1 1 272 8 0.0 1 7 1 273 1829 0.0 1 1738 91 274 128 0.0 1 118 10 275 18 0.0 1 17 1