This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 96.30 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 599,643 (18.8%) Reads that were too short: 35,453 (1.1%) Reads written (passing filters): 564,190 (17.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 116,605,062 bp (12.1%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 599643 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 770 779.6 0 770 7 77 779.6 0 77 8 27 779.6 0 27 9 46 779.6 0 46 10 26 779.6 0 26 11 29 779.6 0 29 12 45 779.6 0 45 13 15 779.6 0 15 14 8 779.6 0 8 15 8 779.6 0 8 16 15 779.6 0 15 17 27 779.6 0 27 18 12 779.6 0 12 19 16 779.6 0 16 20 12 779.6 0 12 21 14 779.6 0 14 22 18 779.6 0 18 23 37 779.6 0 37 24 27 779.6 0 27 25 22 779.6 0 22 26 37 779.6 0 37 27 28 779.6 0 28 28 17 779.6 0 17 29 8 779.6 0 8 30 21 779.6 0 21 31 30 779.6 0 30 32 29 779.6 0 29 33 11 779.6 0 11 34 13 779.6 0 13 35 15 779.6 0 15 36 21 779.6 0 21 37 13 779.6 0 13 38 34 779.6 0 34 39 50 779.6 0 50 40 89 779.6 0 89 41 82 779.6 0 82 42 27 779.6 0 27 43 15 779.6 0 15 44 6 779.6 0 6 45 18 779.6 0 18 46 8 779.6 0 8 47 24 779.6 0 24 48 19 779.6 0 19 49 26 779.6 0 26 50 35 779.6 0 35 51 24 779.6 0 24 52 48 779.6 0 48 53 35 779.6 0 35 54 110 779.6 0 110 55 14 779.6 0 14 56 16 779.6 0 16 57 82 779.6 0 82 58 38 779.6 0 38 59 26 779.6 0 26 60 91 779.6 0 91 61 57 779.6 0 57 62 50 779.6 0 50 63 122 779.6 0 122 64 43 779.6 0 43 65 111 779.6 0 111 66 111 779.6 0 111 67 99 779.6 0 99 68 365 779.6 0 365 69 81 779.6 0 81 70 64 779.6 0 64 71 65 779.6 0 65 72 216 779.6 0 216 73 147 779.6 0 147 74 322 779.6 0 322 75 458 779.6 0 458 76 591 779.6 0 591 77 574 779.6 0 574 78 1300 779.6 0 1300 79 3368 779.6 0 3368 80 822 779.6 0 822 81 1127 779.6 0 1127 82 2090 779.6 0 2090 83 1064 779.6 0 1064 84 980 779.6 0 980 85 2320 779.6 0 2320 86 2731 779.6 0 2731 87 2517 779.6 0 2517 88 2391 779.6 0 2391 89 4520 779.6 0 4520 90 5088 779.6 0 5088 91 6718 779.6 0 6718 92 73663 779.6 0 73663 93 227658 779.6 0 227658 94 206156 779.6 0 206156 95 2199 779.6 0 2199 96 57 779.6 0 57 97 19 779.6 0 19 98 17 779.6 0 17 99 14 779.6 0 14 100 13 779.6 0 13 101 10 779.6 0 10 102 14 779.6 0 14 103 12 779.6 0 12 104 8 779.6 0 8 105 16 779.6 0 16 106 4 779.6 0 4 107 45 779.6 0 45 108 13 779.6 0 13 109 21 779.6 0 21 110 59 779.6 0 59 111 36 779.6 0 36 112 96 779.6 0 96 113 5 779.6 0 5 114 62 779.6 0 62 115 37 779.6 0 37 116 37 779.6 0 37 117 19 779.6 0 19 118 11 779.6 0 11 119 7 779.6 0 7 120 10 779.6 0 10 121 11 779.6 0 11 122 5 779.6 0 5 123 5 779.6 0 5 124 11 779.6 0 11 125 6 779.6 0 6 126 16 779.6 0 16 127 4 779.6 0 4 128 6 779.6 0 6 129 7 779.6 0 7 130 20 779.6 0 20 131 18 779.6 0 18 132 12 779.6 0 12 133 12 779.6 0 12 134 7 779.6 0 7 135 7 779.6 0 7 136 26 779.6 0 26 137 16 779.6 0 16 138 26 779.6 0 26 139 65 779.6 0 65 140 43 779.6 0 43 141 174 779.6 0 174 142 9 779.6 0 9 143 17 779.6 0 17 144 21 779.6 0 21 145 45 779.6 0 45 146 4 779.6 0 4 147 10 779.6 0 10 148 19 779.6 0 19 149 13 779.6 0 13 150 15 779.6 0 15 151 12 779.6 0 12 152 12 779.6 0 12 153 2 779.6 0 2 154 10 779.6 0 10 155 10 779.6 0 10 156 15 779.6 0 15 157 49 779.6 0 49 158 21 779.6 0 21 159 10 779.6 0 10 160 11 779.6 0 11 161 11 779.6 0 11 162 9 779.6 0 9 163 8 779.6 0 8 164 2 779.6 0 2 165 11 779.6 0 11 166 8 779.6 0 8 167 10 779.6 0 10 168 10 779.6 0 10 169 14 779.6 0 14 170 79 779.6 0 79 171 23 779.6 0 23 172 10 779.6 0 10 173 19 779.6 0 19 174 9 779.6 0 9 175 21 779.6 0 21 176 37 779.6 0 37 177 70 779.6 0 70 178 234 779.6 0 234 179 397 779.6 0 397 180 517 779.6 0 517 181 16 779.6 0 16 182 11 779.6 0 11 183 33 779.6 0 33 184 30 779.6 0 30 185 47 779.6 0 47 186 64 779.6 0 64 187 145 779.6 0 145 188 276 779.6 0 276 189 86 779.6 0 86 190 340 779.6 0 340 191 250 779.6 0 250 192 314 779.6 0 314 193 73 779.6 0 73 194 130 779.6 0 130 195 104 779.6 0 104 196 130 779.6 0 130 197 154 779.6 0 154 198 160 779.6 0 160 199 256 779.6 0 256 200 348 779.6 0 348 201 5681 779.6 0 5681 202 5960 779.6 0 5960 203 14521 779.6 0 14521 204 449 779.6 0 449 205 782 779.6 0 782 206 776 779.6 0 776 207 572 779.6 0 572 208 329 779.6 0 329 209 107 779.6 0 107 210 44 779.6 0 44 211 28 779.6 0 28 212 41 779.6 0 41 213 51 779.6 0 51 214 37 779.6 0 37 215 80 779.6 0 80 216 101 779.6 0 101 217 191 779.6 0 191 218 986 779.6 0 986 219 58 779.6 0 58 220 23 779.6 0 23 221 123 779.6 0 123 222 47 779.6 0 47 223 248 779.6 0 248 224 143 779.6 0 143 225 460 779.6 0 460 226 555 779.6 0 555 227 1074 779.6 0 1074 228 514 779.6 0 514 229 202 779.6 0 202 230 16 779.6 0 16 231 7 779.6 0 7 232 8 779.6 0 8 233 5 779.6 0 5 234 9 779.6 0 9 235 12 779.6 0 12 236 45 779.6 0 45 237 70 779.6 0 70 238 22 779.6 0 22 239 7 779.6 0 7 240 1 779.6 0 1 241 3 779.6 0 3 242 6 779.6 0 6 243 11 779.6 0 11 244 4 779.6 0 4 245 18 779.6 0 18 246 15 779.6 0 15 247 36 779.6 0 36 248 21 779.6 0 21 249 15 779.6 0 15 250 12 779.6 0 12 251 23 779.6 0 23 252 11 779.6 0 11 253 13 779.6 0 13 254 24 779.6 0 24 255 30 779.6 0 30 256 37 779.6 0 37 257 73 779.6 0 73 258 56 779.6 0 56 259 73 779.6 0 73 260 141 779.6 0 141 261 229 779.6 0 229 262 161 779.6 0 161 263 1951 779.6 0 1951 264 14 779.6 0 14 265 13 779.6 0 13 266 37 779.6 0 37 267 19 779.6 0 19 268 20 779.6 0 20 269 54 779.6 0 54 270 142 779.6 0 142 271 172 779.6 0 172 272 145 779.6 0 145 273 67 779.6 0 67 274 28 779.6 0 28 275 11 779.6 0 11 276 12 779.6 0 12 277 34 779.6 0 34 278 75 779.6 0 75 279 108 779.6 0 108 280 67 779.6 0 67 281 114 779.6 0 114 282 143 779.6 0 143 283 47 779.6 0 47 284 122 779.6 0 122 285 1044 779.6 0 1044 286 551 779.6 0 551 287 319 779.6 0 319 288 2 779.6 0 2 289 13 779.6 0 13 290 11 779.6 0 11 291 22 779.6 0 22 292 67 779.6 0 67 293 33 779.6 0 33 294 78 779.6 0 78 295 63 779.6 0 63 296 19 779.6 0 19 297 43 779.6 0 43 298 35 779.6 0 35 299 23 779.6 0 23 300 8 779.6 0 8 301 11 779.6 0 11 Finished in 22.08 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 564,190 Reads with adapters: 4,136 (0.7%) Reads that were too short: 254 (0.0%) Reads written (passing filters): 3,882 (0.7%) Total basepairs processed: 116,605,062 bp Total written (filtered): 431,867 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4136 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 1 18 1 0.0 3 0 1 19 40 0.0 3 30 5 5 20 10 0.0 3 6 3 0 1 21 2 0.0 3 0 0 0 2 30 5 0.0 3 0 0 0 5 43 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 1 74 9 0.0 3 0 0 0 9 75 3 0.0 3 0 0 0 3 76 7 0.0 3 0 0 0 7 77 2 0.0 3 0 0 0 2 79 49 0.0 3 0 0 0 49 81 1 0.0 3 0 0 0 1 82 9 0.0 3 0 0 0 9 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 6 0.0 3 0 0 0 6 99 2498 0.0 3 0 0 4 2494 100 203 0.0 3 0 0 0 203 101 7 0.0 3 0 0 0 7 102 958 0.0 3 0 0 11 947 103 68 0.0 3 0 0 2 66 104 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 126 2 0.0 3 0 0 1 1 130 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 6 0.0 3 0 0 0 6 150 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 159 5 0.0 3 0 0 0 5 160 4 0.0 3 0 0 0 4 166 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 7 0.0 3 0 0 1 6 195 1 0.0 3 0 0 0 1 199 5 0.0 3 0 0 0 5 200 128 0.0 3 0 0 0 128 201 64 0.0 3 0 0 0 64 202 4 0.0 3 0 0 0 4 204 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,882 Reads with adapters: 75 (1.9%) Reads written (passing filters): 3,882 (100.0%) Total basepairs processed: 431,867 bp Total written (filtered): 431,636 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 75 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.0% C: 4.0% G: 62.7% T: 17.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 70 60.7 0 70 4 4 15.2 0 4 5 1 3.8 0 1