This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.09 s (29 us/read; 2.06 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 148,551 (4.7%) Reads that were too short: 123,480 (3.9%) Reads written (passing filters): 25,071 (0.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 4,434,496 bp (0.5%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 148551 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 262 779.6 0 262 7 96 779.6 0 96 8 2998 779.6 0 2998 9 12 779.6 0 12 10 4 779.6 0 4 11 40 779.6 0 40 12 52 779.6 0 52 13 6 779.6 0 6 14 5 779.6 0 5 15 7 779.6 0 7 16 5 779.6 0 5 17 9 779.6 0 9 18 7 779.6 0 7 19 5 779.6 0 5 20 9 779.6 0 9 21 7 779.6 0 7 22 5 779.6 0 5 23 5 779.6 0 5 24 5 779.6 0 5 25 12 779.6 0 12 26 17 779.6 0 17 27 15 779.6 0 15 28 3 779.6 0 3 29 4 779.6 0 4 30 3 779.6 0 3 31 5 779.6 0 5 32 1 779.6 0 1 33 5 779.6 0 5 34 6 779.6 0 6 35 4 779.6 0 4 36 7 779.6 0 7 37 7 779.6 0 7 38 4 779.6 0 4 39 5 779.6 0 5 40 3 779.6 0 3 41 7 779.6 0 7 42 17 779.6 0 17 43 5 779.6 0 5 44 3 779.6 0 3 45 10 779.6 0 10 46 5 779.6 0 5 47 4 779.6 0 4 48 5 779.6 0 5 49 3 779.6 0 3 50 8 779.6 0 8 51 6 779.6 0 6 52 8 779.6 0 8 53 8 779.6 0 8 54 5 779.6 0 5 55 5 779.6 0 5 56 7 779.6 0 7 57 8 779.6 0 8 58 3 779.6 0 3 59 11 779.6 0 11 60 12 779.6 0 12 61 6 779.6 0 6 62 41 779.6 0 41 63 9 779.6 0 9 64 4 779.6 0 4 65 5 779.6 0 5 66 7 779.6 0 7 67 4 779.6 0 4 68 18 779.6 0 18 69 8 779.6 0 8 70 33 779.6 0 33 71 140 779.6 0 140 72 921 779.6 0 921 73 48 779.6 0 48 74 22 779.6 0 22 75 17 779.6 0 17 76 92 779.6 0 92 77 120 779.6 0 120 78 144 779.6 0 144 79 119 779.6 0 119 80 256 779.6 0 256 81 193 779.6 0 193 82 103 779.6 0 103 83 177 779.6 0 177 84 180 779.6 0 180 85 229 779.6 0 229 86 56 779.6 0 56 87 31 779.6 0 31 88 48 779.6 0 48 89 59 779.6 0 59 90 168 779.6 0 168 91 38 779.6 0 38 92 82 779.6 0 82 93 169 779.6 0 169 94 233 779.6 0 233 95 185 779.6 0 185 96 86 779.6 0 86 97 22 779.6 0 22 98 27 779.6 0 27 99 62 779.6 0 62 100 129 779.6 0 129 101 84 779.6 0 84 102 66 779.6 0 66 103 40 779.6 0 40 104 41 779.6 0 41 105 208 779.6 0 208 106 47 779.6 0 47 107 29 779.6 0 29 108 29 779.6 0 29 109 54 779.6 0 54 110 101 779.6 0 101 111 89 779.6 0 89 112 102 779.6 0 102 113 49 779.6 0 49 114 84 779.6 0 84 115 114 779.6 0 114 116 73 779.6 0 73 117 81 779.6 0 81 118 149 779.6 0 149 119 225 779.6 0 225 120 138 779.6 0 138 121 159 779.6 0 159 122 131 779.6 0 131 123 158 779.6 0 158 124 185 779.6 0 185 125 215 779.6 0 215 126 509 779.6 0 509 127 252 779.6 0 252 128 183 779.6 0 183 129 61 779.6 0 61 130 63 779.6 0 63 131 118 779.6 0 118 132 115 779.6 0 115 133 117 779.6 0 117 134 123 779.6 0 123 135 159 779.6 0 159 136 194 779.6 0 194 137 176 779.6 0 176 138 205 779.6 0 205 139 126 779.6 0 126 140 197 779.6 0 197 141 220 779.6 0 220 142 85 779.6 0 85 143 71 779.6 0 71 144 167 779.6 0 167 145 242 779.6 0 242 146 92 779.6 0 92 147 117 779.6 0 117 148 83 779.6 0 83 149 74 779.6 0 74 150 87 779.6 0 87 151 80 779.6 0 80 152 155 779.6 0 155 153 239 779.6 0 239 154 215 779.6 0 215 155 158 779.6 0 158 156 152 779.6 0 152 157 136 779.6 0 136 158 196 779.6 0 196 159 119 779.6 0 119 160 142 779.6 0 142 161 113 779.6 0 113 162 96 779.6 0 96 163 114 779.6 0 114 164 203 779.6 0 203 165 186 779.6 0 186 166 146 779.6 0 146 167 114 779.6 0 114 168 93 779.6 0 93 169 221 779.6 0 221 170 101 779.6 0 101 171 123 779.6 0 123 172 184 779.6 0 184 173 412 779.6 0 412 174 418 779.6 0 418 175 234 779.6 0 234 176 86 779.6 0 86 177 141 779.6 0 141 178 196 779.6 0 196 179 214 779.6 0 214 180 294 779.6 0 294 181 198 779.6 0 198 182 138 779.6 0 138 183 135 779.6 0 135 184 107 779.6 0 107 185 181 779.6 0 181 186 103 779.6 0 103 187 69 779.6 0 69 188 60 779.6 0 60 189 144 779.6 0 144 190 420 779.6 0 420 191 488 779.6 0 488 192 201 779.6 0 201 193 158 779.6 0 158 194 91 779.6 0 91 195 75 779.6 0 75 196 253 779.6 0 253 197 1558 779.6 0 1558 198 591 779.6 0 591 199 108 779.6 0 108 200 71 779.6 0 71 201 83 779.6 0 83 202 461 779.6 0 461 203 294 779.6 0 294 204 199 779.6 0 199 205 242 779.6 0 242 206 158 779.6 0 158 207 130 779.6 0 130 208 65 779.6 0 65 209 204 779.6 0 204 210 232 779.6 0 232 211 147 779.6 0 147 212 111 779.6 0 111 213 120 779.6 0 120 214 179 779.6 0 179 215 145 779.6 0 145 216 437 779.6 0 437 217 1644 779.6 0 1644 218 1120 779.6 0 1120 219 460 779.6 0 460 220 80 779.6 0 80 221 93 779.6 0 93 222 186 779.6 0 186 223 539 779.6 0 539 224 415 779.6 0 415 225 204 779.6 0 204 226 334 779.6 0 334 227 585 779.6 0 585 228 633 779.6 0 633 229 4915 779.6 0 4915 230 16426 779.6 0 16426 231 6587 779.6 0 6587 232 108 779.6 0 108 233 111 779.6 0 111 234 169 779.6 0 169 235 193 779.6 0 193 236 72 779.6 0 72 237 82 779.6 0 82 238 93 779.6 0 93 239 118 779.6 0 118 240 72 779.6 0 72 241 71 779.6 0 71 242 74 779.6 0 74 243 125 779.6 0 125 244 273 779.6 0 273 245 430 779.6 0 430 246 348 779.6 0 348 247 180 779.6 0 180 248 83 779.6 0 83 249 34 779.6 0 34 250 59 779.6 0 59 251 155 779.6 0 155 252 237 779.6 0 237 253 256 779.6 0 256 254 302 779.6 0 302 255 276 779.6 0 276 256 186 779.6 0 186 257 107 779.6 0 107 258 134 779.6 0 134 259 892 779.6 0 892 260 997 779.6 0 997 261 1562 779.6 0 1562 262 357 779.6 0 357 263 253 779.6 0 253 264 400 779.6 0 400 265 288 779.6 0 288 266 696 779.6 0 696 267 881 779.6 0 881 268 765 779.6 0 765 269 481 779.6 0 481 270 196 779.6 0 196 271 527 779.6 0 527 272 240 779.6 0 240 273 277 779.6 0 277 274 182 779.6 0 182 275 152 779.6 0 152 276 165 779.6 0 165 277 309 779.6 0 309 278 457 779.6 0 457 279 490 779.6 0 490 280 368 779.6 0 368 281 178 779.6 0 178 282 241 779.6 0 241 283 409 779.6 0 409 284 689 779.6 0 689 285 649 779.6 0 649 286 822 779.6 0 822 287 1098 779.6 0 1098 288 842 779.6 0 842 289 1439 779.6 0 1439 290 462 779.6 0 462 291 439 779.6 0 439 292 1100 779.6 0 1100 293 10353 779.6 0 10353 294 34190 779.6 0 34190 295 17185 779.6 0 17185 296 217 779.6 0 217 297 103 779.6 0 103 298 105 779.6 0 105 299 127 779.6 0 127 300 94 779.6 0 94 301 110 779.6 0 110 Finished in 0.95 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 25,071 Reads with adapters: 3,346 (13.3%) Reads that were too short: 14 (0.1%) Reads written (passing filters): 3,332 (13.3%) Total basepairs processed: 4,434,496 bp Total written (filtered): 914,641 bp (20.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3346 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 24 0.0 3 0 22 2 17 3028 0.0 3 2957 66 4 1 18 16 0.0 3 1 5 4 6 19 18 0.0 3 4 1 11 2 20 219 0.0 3 204 6 0 9 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,332 Reads with adapters: 230 (6.9%) Reads written (passing filters): 3,332 (100.0%) Total basepairs processed: 914,641 bp Total written (filtered): 913,914 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 230 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.6% C: 16.1% G: 12.2% T: 19.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 195 52.1 0 195 4 34 13.0 0 34 6 1 0.8 0 1