This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.79 s (29 us/read; 2.06 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 398,045 (12.5%) Reads that were too short: 205,342 (6.4%) Reads written (passing filters): 192,703 (6.0%) Total basepairs processed: 961,105,341 bp Total written (filtered): 32,562,298 bp (3.4%) === Adapter 1 === Sequence: AACAAC; Type: regular 5'; Length: 6; Trimmed: 398045 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 47 779.6 0 47 7 888 779.6 0 888 8 19 779.6 0 19 9 21 779.6 0 21 10 21 779.6 0 21 11 16 779.6 0 16 12 17 779.6 0 17 13 19 779.6 0 19 14 13 779.6 0 13 15 12 779.6 0 12 16 11 779.6 0 11 17 21 779.6 0 21 18 6 779.6 0 6 19 8 779.6 0 8 20 9 779.6 0 9 21 13 779.6 0 13 22 10 779.6 0 10 23 13 779.6 0 13 24 11 779.6 0 11 25 10 779.6 0 10 26 9 779.6 0 9 27 8 779.6 0 8 28 11 779.6 0 11 29 5 779.6 0 5 30 14 779.6 0 14 31 11 779.6 0 11 32 13 779.6 0 13 33 8 779.6 0 8 34 12 779.6 0 12 35 12 779.6 0 12 36 14 779.6 0 14 37 14 779.6 0 14 38 13 779.6 0 13 39 13 779.6 0 13 40 11 779.6 0 11 41 11 779.6 0 11 42 16 779.6 0 16 43 18 779.6 0 18 44 13 779.6 0 13 45 13 779.6 0 13 46 9 779.6 0 9 47 11 779.6 0 11 48 5 779.6 0 5 49 10 779.6 0 10 50 17 779.6 0 17 51 8 779.6 0 8 52 6 779.6 0 6 53 14 779.6 0 14 54 14 779.6 0 14 55 17 779.6 0 17 56 9 779.6 0 9 57 15 779.6 0 15 58 10 779.6 0 10 59 9 779.6 0 9 60 10 779.6 0 10 61 14 779.6 0 14 62 42 779.6 0 42 63 89 779.6 0 89 64 157 779.6 0 157 65 206 779.6 0 206 66 612 779.6 0 612 67 638 779.6 0 638 68 128 779.6 0 128 69 256 779.6 0 256 70 593 779.6 0 593 71 2136 779.6 0 2136 72 2693 779.6 0 2693 73 1700 779.6 0 1700 74 2233 779.6 0 2233 75 758 779.6 0 758 76 1351 779.6 0 1351 77 2330 779.6 0 2330 78 1527 779.6 0 1527 79 1539 779.6 0 1539 80 1267 779.6 0 1267 81 1167 779.6 0 1167 82 767 779.6 0 767 83 1090 779.6 0 1090 84 792 779.6 0 792 85 728 779.6 0 728 86 688 779.6 0 688 87 2665 779.6 0 2665 88 656 779.6 0 656 89 653 779.6 0 653 90 4907 779.6 0 4907 91 1790 779.6 0 1790 92 788 779.6 0 788 93 1575 779.6 0 1575 94 1065 779.6 0 1065 95 512 779.6 0 512 96 667 779.6 0 667 97 824 779.6 0 824 98 537 779.6 0 537 99 739 779.6 0 739 100 1731 779.6 0 1731 101 1691 779.6 0 1691 102 1244 779.6 0 1244 103 2472 779.6 0 2472 104 2651 779.6 0 2651 105 1141 779.6 0 1141 106 1543 779.6 0 1543 107 2529 779.6 0 2529 108 822 779.6 0 822 109 1030 779.6 0 1030 110 2026 779.6 0 2026 111 1686 779.6 0 1686 112 1710 779.6 0 1710 113 1555 779.6 0 1555 114 1077 779.6 0 1077 115 1274 779.6 0 1274 116 2582 779.6 0 2582 117 1047 779.6 0 1047 118 1047 779.6 0 1047 119 2549 779.6 0 2549 120 1678 779.6 0 1678 121 1287 779.6 0 1287 122 2971 779.6 0 2971 123 1837 779.6 0 1837 124 2535 779.6 0 2535 125 2657 779.6 0 2657 126 1704 779.6 0 1704 127 1217 779.6 0 1217 128 1683 779.6 0 1683 129 1136 779.6 0 1136 130 1081 779.6 0 1081 131 1680 779.6 0 1680 132 1428 779.6 0 1428 133 1145 779.6 0 1145 134 1113 779.6 0 1113 135 1085 779.6 0 1085 136 1078 779.6 0 1078 137 987 779.6 0 987 138 1078 779.6 0 1078 139 928 779.6 0 928 140 849 779.6 0 849 141 1274 779.6 0 1274 142 1799 779.6 0 1799 143 1043 779.6 0 1043 144 1281 779.6 0 1281 145 1618 779.6 0 1618 146 1428 779.6 0 1428 147 1200 779.6 0 1200 148 1011 779.6 0 1011 149 1662 779.6 0 1662 150 1755 779.6 0 1755 151 2036 779.6 0 2036 152 1777 779.6 0 1777 153 1856 779.6 0 1856 154 2345 779.6 0 2345 155 1320 779.6 0 1320 156 1034 779.6 0 1034 157 1222 779.6 0 1222 158 1274 779.6 0 1274 159 1307 779.6 0 1307 160 1148 779.6 0 1148 161 1288 779.6 0 1288 162 1425 779.6 0 1425 163 1390 779.6 0 1390 164 1127 779.6 0 1127 165 918 779.6 0 918 166 859 779.6 0 859 167 977 779.6 0 977 168 972 779.6 0 972 169 1120 779.6 0 1120 170 1915 779.6 0 1915 171 1774 779.6 0 1774 172 1193 779.6 0 1193 173 1578 779.6 0 1578 174 1606 779.6 0 1606 175 1474 779.6 0 1474 176 1338 779.6 0 1338 177 1630 779.6 0 1630 178 1789 779.6 0 1789 179 1970 779.6 0 1970 180 1626 779.6 0 1626 181 1191 779.6 0 1191 182 1684 779.6 0 1684 183 1287 779.6 0 1287 184 992 779.6 0 992 185 898 779.6 0 898 186 885 779.6 0 885 187 1216 779.6 0 1216 188 1420 779.6 0 1420 189 1388 779.6 0 1388 190 1376 779.6 0 1376 191 1696 779.6 0 1696 192 1314 779.6 0 1314 193 887 779.6 0 887 194 1431 779.6 0 1431 195 1672 779.6 0 1672 196 939 779.6 0 939 197 1035 779.6 0 1035 198 1363 779.6 0 1363 199 1379 779.6 0 1379 200 1249 779.6 0 1249 201 1011 779.6 0 1011 202 915 779.6 0 915 203 1309 779.6 0 1309 204 1465 779.6 0 1465 205 808 779.6 0 808 206 828 779.6 0 828 207 1535 779.6 0 1535 208 841 779.6 0 841 209 793 779.6 0 793 210 1345 779.6 0 1345 211 1042 779.6 0 1042 212 979 779.6 0 979 213 1147 779.6 0 1147 214 1593 779.6 0 1593 215 1884 779.6 0 1884 216 1009 779.6 0 1009 217 1159 779.6 0 1159 218 1220 779.6 0 1220 219 1463 779.6 0 1463 220 1277 779.6 0 1277 221 1209 779.6 0 1209 222 870 779.6 0 870 223 1211 779.6 0 1211 224 1796 779.6 0 1796 225 1568 779.6 0 1568 226 2599 779.6 0 2599 227 4706 779.6 0 4706 228 5587 779.6 0 5587 229 1593 779.6 0 1593 230 2370 779.6 0 2370 231 5431 779.6 0 5431 232 5555 779.6 0 5555 233 1254 779.6 0 1254 234 1095 779.6 0 1095 235 1905 779.6 0 1905 236 1110 779.6 0 1110 237 884 779.6 0 884 238 1201 779.6 0 1201 239 1385 779.6 0 1385 240 1494 779.6 0 1494 241 1517 779.6 0 1517 242 1164 779.6 0 1164 243 1300 779.6 0 1300 244 1128 779.6 0 1128 245 941 779.6 0 941 246 764 779.6 0 764 247 878 779.6 0 878 248 972 779.6 0 972 249 1319 779.6 0 1319 250 1318 779.6 0 1318 251 1662 779.6 0 1662 252 1926 779.6 0 1926 253 1470 779.6 0 1470 254 1278 779.6 0 1278 255 907 779.6 0 907 256 771 779.6 0 771 257 664 779.6 0 664 258 829 779.6 0 829 259 912 779.6 0 912 260 1441 779.6 0 1441 261 1975 779.6 0 1975 262 2563 779.6 0 2563 263 11444 779.6 0 11444 264 15432 779.6 0 15432 265 17496 779.6 0 17496 266 16034 779.6 0 16034 267 1355 779.6 0 1355 268 1192 779.6 0 1192 269 1205 779.6 0 1205 270 1467 779.6 0 1467 271 1371 779.6 0 1371 272 1183 779.6 0 1183 273 1291 779.6 0 1291 274 1642 779.6 0 1642 275 1965 779.6 0 1965 276 1624 779.6 0 1624 277 1612 779.6 0 1612 278 1861 779.6 0 1861 279 1840 779.6 0 1840 280 1701 779.6 0 1701 281 1504 779.6 0 1504 282 1128 779.6 0 1128 283 2007 779.6 0 2007 284 1423 779.6 0 1423 285 1100 779.6 0 1100 286 1717 779.6 0 1717 287 1266 779.6 0 1266 288 1125 779.6 0 1125 289 1513 779.6 0 1513 290 2241 779.6 0 2241 291 3458 779.6 0 3458 292 2345 779.6 0 2345 293 1378 779.6 0 1378 294 1117 779.6 0 1117 295 901 779.6 0 901 296 1166 779.6 0 1166 297 799 779.6 0 799 298 728 779.6 0 728 299 676 779.6 0 676 300 540 779.6 0 540 301 361 779.6 0 361 Finished in 6.51 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 192,703 Reads with adapters: 1,112 (0.6%) Reads that were too short: 59 (0.0%) Reads written (passing filters): 1,053 (0.5%) Total basepairs processed: 32,562,298 bp Total written (filtered): 264,632 bp (0.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1112 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 5 0.0 3 0 5 17 874 0.0 3 841 23 6 4 18 4 0.0 3 1 2 1 19 2 0.0 3 1 0 0 1 20 11 0.0 3 0 0 0 11 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 6 0.0 3 0 0 0 6 24 2 0.0 3 0 0 0 2 26 3 0.0 3 0 0 0 3 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 59 0.0 3 0 0 0 59 70 1 0.0 3 0 0 0 1 71 38 0.0 3 0 0 0 38 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 1 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 1 106 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 3 0.0 3 0 0 0 3 123 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 126 13 0.0 3 0 0 0 13 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 10 0.0 3 0 1 0 9 130 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 1,053 Reads with adapters: 135 (12.8%) Reads written (passing filters): 1,053 (100.0%) Total basepairs processed: 264,632 bp Total written (filtered): 264,224 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 135 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.1% C: 37.8% G: 16.3% T: 17.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 132 16.5 0 132 4 3 4.1 0 3