This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.55 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 71,240 (2.2%) Reads that were too short: 13,289 (0.4%) Reads written (passing filters): 57,951 (1.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 14,234,827 bp (1.5%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 71240 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 188 779.6 0 188 7 764 779.6 0 764 8 420 779.6 0 420 9 22265 779.6 0 22265 10 25 779.6 0 25 11 6 779.6 0 6 12 6 779.6 0 6 13 4 779.6 0 4 14 3 779.6 0 3 15 8 779.6 0 8 16 7 779.6 0 7 17 7 779.6 0 7 18 4 779.6 0 4 19 6 779.6 0 6 20 12 779.6 0 12 21 7 779.6 0 7 22 5 779.6 0 5 23 13 779.6 0 13 24 13 779.6 0 13 25 16 779.6 0 16 26 14 779.6 0 14 27 25 779.6 0 25 28 33 779.6 0 33 29 137 779.6 0 137 30 267 779.6 0 267 31 4 779.6 0 4 32 10 779.6 0 10 33 142 779.6 0 142 34 629 779.6 0 629 35 200 779.6 0 200 36 20 779.6 0 20 37 48 779.6 0 48 38 8 779.6 0 8 39 19 779.6 0 19 40 11 779.6 0 11 41 20 779.6 0 20 42 32 779.6 0 32 43 16 779.6 0 16 44 22 779.6 0 22 45 13 779.6 0 13 46 26 779.6 0 26 47 25 779.6 0 25 48 34 779.6 0 34 49 49 779.6 0 49 50 90 779.6 0 90 51 118 779.6 0 118 52 272 779.6 0 272 53 1340 779.6 0 1340 54 5310 779.6 0 5310 55 3879 779.6 0 3879 56 4932 779.6 0 4932 57 28 779.6 0 28 58 8 779.6 0 8 59 4 779.6 0 4 60 11 779.6 0 11 61 4 779.6 0 4 62 2 779.6 0 2 63 4 779.6 0 4 64 11 779.6 0 11 65 3 779.6 0 3 66 2 779.6 0 2 67 4 779.6 0 4 68 4 779.6 0 4 69 10 779.6 0 10 70 9 779.6 0 9 71 5 779.6 0 5 72 7 779.6 0 7 73 9 779.6 0 9 74 13 779.6 0 13 75 58 779.6 0 58 76 10 779.6 0 10 77 13 779.6 0 13 78 26 779.6 0 26 79 20 779.6 0 20 80 37 779.6 0 37 81 96 779.6 0 96 82 50 779.6 0 50 83 48 779.6 0 48 84 38 779.6 0 38 85 41 779.6 0 41 86 30 779.6 0 30 87 75 779.6 0 75 88 164 779.6 0 164 89 47 779.6 0 47 90 50 779.6 0 50 91 99 779.6 0 99 92 109 779.6 0 109 93 121 779.6 0 121 94 853 779.6 0 853 95 1515 779.6 0 1515 96 3841 779.6 0 3841 97 66 779.6 0 66 98 77 779.6 0 77 99 27 779.6 0 27 100 10 779.6 0 10 101 13 779.6 0 13 102 17 779.6 0 17 103 18 779.6 0 18 104 29 779.6 0 29 105 16 779.6 0 16 106 5 779.6 0 5 107 18 779.6 0 18 108 275 779.6 0 275 109 68 779.6 0 68 110 53 779.6 0 53 111 252 779.6 0 252 112 21 779.6 0 21 113 31 779.6 0 31 114 17 779.6 0 17 115 18 779.6 0 18 116 50 779.6 0 50 117 74 779.6 0 74 118 31 779.6 0 31 119 163 779.6 0 163 120 89 779.6 0 89 121 483 779.6 0 483 122 221 779.6 0 221 123 366 779.6 0 366 124 51 779.6 0 51 125 30 779.6 0 30 126 37 779.6 0 37 127 23 779.6 0 23 128 41 779.6 0 41 129 83 779.6 0 83 130 50 779.6 0 50 131 31 779.6 0 31 132 25 779.6 0 25 133 37 779.6 0 37 134 50 779.6 0 50 135 42 779.6 0 42 136 56 779.6 0 56 137 54 779.6 0 54 138 58 779.6 0 58 139 82 779.6 0 82 140 85 779.6 0 85 141 118 779.6 0 118 142 61 779.6 0 61 143 59 779.6 0 59 144 28 779.6 0 28 145 52 779.6 0 52 146 47 779.6 0 47 147 102 779.6 0 102 148 78 779.6 0 78 149 46 779.6 0 46 150 73 779.6 0 73 151 59 779.6 0 59 152 47 779.6 0 47 153 36 779.6 0 36 154 32 779.6 0 32 155 84 779.6 0 84 156 53 779.6 0 53 157 63 779.6 0 63 158 67 779.6 0 67 159 79 779.6 0 79 160 124 779.6 0 124 161 152 779.6 0 152 162 165 779.6 0 165 163 111 779.6 0 111 164 43 779.6 0 43 165 78 779.6 0 78 166 94 779.6 0 94 167 143 779.6 0 143 168 74 779.6 0 74 169 41 779.6 0 41 170 28 779.6 0 28 171 47 779.6 0 47 172 139 779.6 0 139 173 26 779.6 0 26 174 31 779.6 0 31 175 86 779.6 0 86 176 591 779.6 0 591 177 513 779.6 0 513 178 664 779.6 0 664 179 33 779.6 0 33 180 38 779.6 0 38 181 53 779.6 0 53 182 96 779.6 0 96 183 65 779.6 0 65 184 44 779.6 0 44 185 37 779.6 0 37 186 48 779.6 0 48 187 52 779.6 0 52 188 88 779.6 0 88 189 62 779.6 0 62 190 38 779.6 0 38 191 20 779.6 0 20 192 56 779.6 0 56 193 54 779.6 0 54 194 52 779.6 0 52 195 78 779.6 0 78 196 128 779.6 0 128 197 34 779.6 0 34 198 10 779.6 0 10 199 20 779.6 0 20 200 68 779.6 0 68 201 97 779.6 0 97 202 56 779.6 0 56 203 33 779.6 0 33 204 46 779.6 0 46 205 61 779.6 0 61 206 35 779.6 0 35 207 82 779.6 0 82 208 75 779.6 0 75 209 78 779.6 0 78 210 39 779.6 0 39 211 38 779.6 0 38 212 69 779.6 0 69 213 83 779.6 0 83 214 61 779.6 0 61 215 65 779.6 0 65 216 68 779.6 0 68 217 43 779.6 0 43 218 29 779.6 0 29 219 35 779.6 0 35 220 79 779.6 0 79 221 237 779.6 0 237 222 123 779.6 0 123 223 49 779.6 0 49 224 35 779.6 0 35 225 57 779.6 0 57 226 126 779.6 0 126 227 64 779.6 0 64 228 61 779.6 0 61 229 79 779.6 0 79 230 67 779.6 0 67 231 47 779.6 0 47 232 114 779.6 0 114 233 147 779.6 0 147 234 49 779.6 0 49 235 19 779.6 0 19 236 50 779.6 0 50 237 90 779.6 0 90 238 86 779.6 0 86 239 69 779.6 0 69 240 61 779.6 0 61 241 74 779.6 0 74 242 86 779.6 0 86 243 42 779.6 0 42 244 46 779.6 0 46 245 49 779.6 0 49 246 57 779.6 0 57 247 80 779.6 0 80 248 124 779.6 0 124 249 143 779.6 0 143 250 155 779.6 0 155 251 141 779.6 0 141 252 85 779.6 0 85 253 51 779.6 0 51 254 56 779.6 0 56 255 61 779.6 0 61 256 81 779.6 0 81 257 162 779.6 0 162 258 160 779.6 0 160 259 140 779.6 0 140 260 127 779.6 0 127 261 162 779.6 0 162 262 369 779.6 0 369 263 1055 779.6 0 1055 264 401 779.6 0 401 265 63 779.6 0 63 266 49 779.6 0 49 267 37 779.6 0 37 268 74 779.6 0 74 269 123 779.6 0 123 270 115 779.6 0 115 271 105 779.6 0 105 272 80 779.6 0 80 273 84 779.6 0 84 274 65 779.6 0 65 275 154 779.6 0 154 276 143 779.6 0 143 277 172 779.6 0 172 278 789 779.6 0 789 279 146 779.6 0 146 280 164 779.6 0 164 281 122 779.6 0 122 282 364 779.6 0 364 283 452 779.6 0 452 284 237 779.6 0 237 285 57 779.6 0 57 286 67 779.6 0 67 287 182 779.6 0 182 288 609 779.6 0 609 289 496 779.6 0 496 290 127 779.6 0 127 291 190 779.6 0 190 292 120 779.6 0 120 293 100 779.6 0 100 294 126 779.6 0 126 295 353 779.6 0 353 296 179 779.6 0 179 297 74 779.6 0 74 298 166 779.6 0 166 299 108 779.6 0 108 300 114 779.6 0 114 301 71 779.6 0 71 Finished in 2.72 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 57,951 Reads with adapters: 23,748 (41.0%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 23,731 (41.0%) Total basepairs processed: 14,234,827 bp Total written (filtered): 6,490,945 bp (45.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23748 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 9 0.0 3 0 0 9 16 225 0.0 3 0 219 3 3 17 22542 0.0 3 21742 708 75 17 18 77 0.0 3 6 59 6 6 19 612 0.0 3 572 14 18 8 20 26 0.0 3 0 3 0 23 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 49 4 0.0 3 0 0 0 4 50 28 0.0 3 0 0 0 28 51 3 0.0 3 0 0 0 3 56 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 72 35 0.0 3 0 0 0 35 75 1 0.0 3 0 0 0 1 79 1 0.0 3 1 97 6 0.0 3 0 0 0 6 98 112 0.0 3 0 0 0 112 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 114 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 140 4 0.0 3 0 0 0 4 154 1 0.0 3 0 0 0 1 158 12 0.0 3 0 0 0 12 160 1 0.0 3 0 0 0 1 161 12 0.0 3 0 0 1 11 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.99 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 23,731 Reads with adapters: 2,154 (9.1%) Reads written (passing filters): 23,731 (100.0%) Total basepairs processed: 6,490,945 bp Total written (filtered): 6,484,308 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2154 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.2% C: 6.2% G: 27.4% T: 11.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1988 370.8 0 1988 4 163 92.7 0 163 5 1 23.2 0 1 6 1 5.8 0 1 10 1 0.0 1 0 1