This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.74 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 220,720 (6.9%) Reads that were too short: 57,748 (1.8%) Reads written (passing filters): 162,972 (5.1%) Total basepairs processed: 961,105,341 bp Total written (filtered): 37,056,706 bp (3.9%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 220720 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 567 779.6 0 567 7 1231 779.6 0 1231 8 73278 779.6 0 73278 9 79 779.6 0 79 10 10 779.6 0 10 11 14 779.6 0 14 12 13 779.6 0 13 13 6 779.6 0 6 14 10 779.6 0 10 15 9 779.6 0 9 16 8 779.6 0 8 17 6 779.6 0 6 18 6 779.6 0 6 19 5 779.6 0 5 20 9 779.6 0 9 21 9 779.6 0 9 22 4 779.6 0 4 23 9 779.6 0 9 24 12 779.6 0 12 25 7 779.6 0 7 26 5 779.6 0 5 27 11 779.6 0 11 28 14 779.6 0 14 29 11 779.6 0 11 30 9 779.6 0 9 31 9 779.6 0 9 32 20 779.6 0 20 33 5 779.6 0 5 34 8 779.6 0 8 35 9 779.6 0 9 36 4 779.6 0 4 37 12 779.6 0 12 38 10 779.6 0 10 39 11 779.6 0 11 40 7 779.6 0 7 41 8 779.6 0 8 42 10 779.6 0 10 43 6 779.6 0 6 44 8 779.6 0 8 45 12 779.6 0 12 46 12 779.6 0 12 47 29 779.6 0 29 48 6 779.6 0 6 49 7 779.6 0 7 50 13 779.6 0 13 51 9 779.6 0 9 52 10 779.6 0 10 53 6 779.6 0 6 54 13 779.6 0 13 55 15 779.6 0 15 56 15 779.6 0 15 57 6 779.6 0 6 58 31 779.6 0 31 59 12 779.6 0 12 60 12 779.6 0 12 61 18 779.6 0 18 62 38 779.6 0 38 63 25 779.6 0 25 64 24 779.6 0 24 65 41 779.6 0 41 66 110 779.6 0 110 67 30 779.6 0 30 68 41 779.6 0 41 69 167 779.6 0 167 70 53 779.6 0 53 71 88 779.6 0 88 72 453 779.6 0 453 73 401 779.6 0 401 74 114 779.6 0 114 75 154 779.6 0 154 76 430 779.6 0 430 77 1335 779.6 0 1335 78 646 779.6 0 646 79 2045 779.6 0 2045 80 1473 779.6 0 1473 81 5796 779.6 0 5796 82 1003 779.6 0 1003 83 1034 779.6 0 1034 84 1925 779.6 0 1925 85 1350 779.6 0 1350 86 1088 779.6 0 1088 87 208 779.6 0 208 88 166 779.6 0 166 89 542 779.6 0 542 90 476 779.6 0 476 91 148 779.6 0 148 92 262 779.6 0 262 93 630 779.6 0 630 94 1011 779.6 0 1011 95 1561 779.6 0 1561 96 771 779.6 0 771 97 205 779.6 0 205 98 163 779.6 0 163 99 701 779.6 0 701 100 1143 779.6 0 1143 101 756 779.6 0 756 102 354 779.6 0 354 103 284 779.6 0 284 104 97 779.6 0 97 105 351 779.6 0 351 106 915 779.6 0 915 107 208 779.6 0 208 108 80 779.6 0 80 109 244 779.6 0 244 110 491 779.6 0 491 111 538 779.6 0 538 112 595 779.6 0 595 113 438 779.6 0 438 114 421 779.6 0 421 115 761 779.6 0 761 116 539 779.6 0 539 117 457 779.6 0 457 118 1108 779.6 0 1108 119 835 779.6 0 835 120 608 779.6 0 608 121 409 779.6 0 409 122 383 779.6 0 383 123 424 779.6 0 424 124 538 779.6 0 538 125 689 779.6 0 689 126 1013 779.6 0 1013 127 1191 779.6 0 1191 128 477 779.6 0 477 129 396 779.6 0 396 130 777 779.6 0 777 131 941 779.6 0 941 132 709 779.6 0 709 133 492 779.6 0 492 134 448 779.6 0 448 135 523 779.6 0 523 136 797 779.6 0 797 137 882 779.6 0 882 138 727 779.6 0 727 139 621 779.6 0 621 140 592 779.6 0 592 141 1273 779.6 0 1273 142 512 779.6 0 512 143 802 779.6 0 802 144 549 779.6 0 549 145 896 779.6 0 896 146 588 779.6 0 588 147 460 779.6 0 460 148 498 779.6 0 498 149 562 779.6 0 562 150 440 779.6 0 440 151 346 779.6 0 346 152 558 779.6 0 558 153 654 779.6 0 654 154 763 779.6 0 763 155 335 779.6 0 335 156 389 779.6 0 389 157 657 779.6 0 657 158 1170 779.6 0 1170 159 1017 779.6 0 1017 160 886 779.6 0 886 161 334 779.6 0 334 162 476 779.6 0 476 163 410 779.6 0 410 164 484 779.6 0 484 165 456 779.6 0 456 166 810 779.6 0 810 167 380 779.6 0 380 168 457 779.6 0 457 169 579 779.6 0 579 170 639 779.6 0 639 171 269 779.6 0 269 172 250 779.6 0 250 173 555 779.6 0 555 174 776 779.6 0 776 175 378 779.6 0 378 176 206 779.6 0 206 177 229 779.6 0 229 178 347 779.6 0 347 179 687 779.6 0 687 180 625 779.6 0 625 181 458 779.6 0 458 182 867 779.6 0 867 183 1476 779.6 0 1476 184 1546 779.6 0 1546 185 614 779.6 0 614 186 584 779.6 0 584 187 505 779.6 0 505 188 268 779.6 0 268 189 206 779.6 0 206 190 385 779.6 0 385 191 722 779.6 0 722 192 727 779.6 0 727 193 630 779.6 0 630 194 557 779.6 0 557 195 306 779.6 0 306 196 227 779.6 0 227 197 552 779.6 0 552 198 1562 779.6 0 1562 199 963 779.6 0 963 200 305 779.6 0 305 201 155 779.6 0 155 202 289 779.6 0 289 203 668 779.6 0 668 204 646 779.6 0 646 205 612 779.6 0 612 206 691 779.6 0 691 207 422 779.6 0 422 208 269 779.6 0 269 209 340 779.6 0 340 210 653 779.6 0 653 211 690 779.6 0 690 212 447 779.6 0 447 213 371 779.6 0 371 214 437 779.6 0 437 215 370 779.6 0 370 216 302 779.6 0 302 217 419 779.6 0 419 218 545 779.6 0 545 219 521 779.6 0 521 220 377 779.6 0 377 221 194 779.6 0 194 222 248 779.6 0 248 223 466 779.6 0 466 224 429 779.6 0 429 225 319 779.6 0 319 226 416 779.6 0 416 227 683 779.6 0 683 228 523 779.6 0 523 229 372 779.6 0 372 230 451 779.6 0 451 231 523 779.6 0 523 232 246 779.6 0 246 233 349 779.6 0 349 234 741 779.6 0 741 235 903 779.6 0 903 236 552 779.6 0 552 237 526 779.6 0 526 238 612 779.6 0 612 239 777 779.6 0 777 240 476 779.6 0 476 241 402 779.6 0 402 242 460 779.6 0 460 243 471 779.6 0 471 244 379 779.6 0 379 245 353 779.6 0 353 246 445 779.6 0 445 247 489 779.6 0 489 248 525 779.6 0 525 249 518 779.6 0 518 250 467 779.6 0 467 251 1040 779.6 0 1040 252 665 779.6 0 665 253 498 779.6 0 498 254 783 779.6 0 783 255 989 779.6 0 989 256 993 779.6 0 993 257 396 779.6 0 396 258 347 779.6 0 347 259 327 779.6 0 327 260 461 779.6 0 461 261 516 779.6 0 516 262 475 779.6 0 475 263 319 779.6 0 319 264 368 779.6 0 368 265 420 779.6 0 420 266 399 779.6 0 399 267 610 779.6 0 610 268 451 779.6 0 451 269 281 779.6 0 281 270 358 779.6 0 358 271 617 779.6 0 617 272 688 779.6 0 688 273 831 779.6 0 831 274 1020 779.6 0 1020 275 619 779.6 0 619 276 526 779.6 0 526 277 1028 779.6 0 1028 278 946 779.6 0 946 279 635 779.6 0 635 280 469 779.6 0 469 281 363 779.6 0 363 282 410 779.6 0 410 283 472 779.6 0 472 284 444 779.6 0 444 285 602 779.6 0 602 286 551 779.6 0 551 287 379 779.6 0 379 288 861 779.6 0 861 289 1120 779.6 0 1120 290 1063 779.6 0 1063 291 736 779.6 0 736 292 638 779.6 0 638 293 689 779.6 0 689 294 620 779.6 0 620 295 525 779.6 0 525 296 663 779.6 0 663 297 1002 779.6 0 1002 298 571 779.6 0 571 299 626 779.6 0 626 300 1792 779.6 0 1792 301 2162 779.6 0 2162 Finished in 7.04 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 162,972 Reads with adapters: 74,945 (46.0%) Reads that were too short: 55 (0.0%) Reads written (passing filters): 74,890 (46.0%) Total basepairs processed: 37,056,706 bp Total written (filtered): 20,658,242 bp (55.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 74945 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 37 0.0 3 0 0 26 11 16 781 0.0 3 0 722 38 21 17 73296 0.0 3 71052 1976 204 64 18 196 0.0 3 17 163 8 8 19 392 0.0 3 2 0 328 62 20 107 0.0 3 0 0 1 106 21 6 0.0 3 0 0 0 6 25 12 0.0 3 1 0 0 11 28 7 0.0 3 1 0 0 6 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 1 39 1 0.0 3 0 0 0 1 41 7 0.0 3 0 0 0 7 44 4 0.0 3 0 0 1 3 50 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 65 4 0.0 3 0 0 0 4 67 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 3 0.0 3 0 0 0 3 109 4 0.0 3 0 0 0 4 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 7 0.0 3 0 0 0 7 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 8 0.0 3 0 0 0 8 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 214 20 0.0 3 0 0 3 17 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.37 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 74,890 Reads with adapters: 6,651 (8.9%) Reads written (passing filters): 74,890 (100.0%) Total basepairs processed: 20,658,242 bp Total written (filtered): 20,637,879 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6651 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.4% C: 5.1% G: 54.5% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6257 1170.2 0 6257 4 381 292.5 0 381 5 10 73.1 0 10 6 3 18.3 0 3