This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.11 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 122,509 (3.8%) Reads that were too short: 15,978 (0.5%) Reads written (passing filters): 106,531 (3.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 29,107,238 bp (3.0%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 122509 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3549 779.6 0 3549 7 78184 779.6 0 78184 8 271 779.6 0 271 9 7884 779.6 0 7884 10 19 779.6 0 19 11 5 779.6 0 5 12 1 779.6 0 1 13 2 779.6 0 2 14 2 779.6 0 2 15 621 779.6 0 621 16 84 779.6 0 84 18 5 779.6 0 5 19 2 779.6 0 2 20 5 779.6 0 5 21 9 779.6 0 9 22 6 779.6 0 6 23 9 779.6 0 9 25 1 779.6 0 1 27 3 779.6 0 3 28 6 779.6 0 6 29 3 779.6 0 3 30 2 779.6 0 2 31 4 779.6 0 4 32 3 779.6 0 3 33 15 779.6 0 15 34 7 779.6 0 7 35 1 779.6 0 1 36 4 779.6 0 4 37 1 779.6 0 1 38 3 779.6 0 3 39 4 779.6 0 4 40 4 779.6 0 4 41 30 779.6 0 30 42 10 779.6 0 10 43 16 779.6 0 16 44 2 779.6 0 2 45 3 779.6 0 3 46 3 779.6 0 3 47 2 779.6 0 2 48 1 779.6 0 1 49 2 779.6 0 2 50 2 779.6 0 2 51 9 779.6 0 9 52 7 779.6 0 7 53 4 779.6 0 4 54 5 779.6 0 5 55 4 779.6 0 4 56 12 779.6 0 12 57 12 779.6 0 12 58 9 779.6 0 9 59 4 779.6 0 4 60 4 779.6 0 4 61 9 779.6 0 9 62 3 779.6 0 3 63 6 779.6 0 6 64 9 779.6 0 9 65 14 779.6 0 14 66 10 779.6 0 10 67 17 779.6 0 17 68 20 779.6 0 20 69 223 779.6 0 223 70 454 779.6 0 454 71 456 779.6 0 456 72 4 779.6 0 4 73 3 779.6 0 3 74 8 779.6 0 8 75 6 779.6 0 6 76 2 779.6 0 2 77 9 779.6 0 9 78 5 779.6 0 5 79 7 779.6 0 7 80 84 779.6 0 84 81 21 779.6 0 21 82 39 779.6 0 39 83 16 779.6 0 16 84 30 779.6 0 30 85 21 779.6 0 21 86 19 779.6 0 19 87 11 779.6 0 11 88 9 779.6 0 9 89 30 779.6 0 30 90 9 779.6 0 9 91 24 779.6 0 24 92 15 779.6 0 15 93 9 779.6 0 9 94 10 779.6 0 10 95 7 779.6 0 7 96 11 779.6 0 11 97 27 779.6 0 27 98 16 779.6 0 16 99 70 779.6 0 70 100 98 779.6 0 98 101 145 779.6 0 145 102 472 779.6 0 472 103 235 779.6 0 235 104 69 779.6 0 69 105 23 779.6 0 23 106 15 779.6 0 15 107 8 779.6 0 8 108 29 779.6 0 29 109 17 779.6 0 17 110 6 779.6 0 6 111 22 779.6 0 22 112 21 779.6 0 21 113 23 779.6 0 23 114 17 779.6 0 17 115 22 779.6 0 22 116 20 779.6 0 20 117 29 779.6 0 29 118 54 779.6 0 54 119 45 779.6 0 45 120 67 779.6 0 67 121 58 779.6 0 58 122 62 779.6 0 62 123 151 779.6 0 151 124 124 779.6 0 124 125 56 779.6 0 56 126 76 779.6 0 76 127 25 779.6 0 25 128 27 779.6 0 27 129 22 779.6 0 22 130 51 779.6 0 51 131 44 779.6 0 44 132 117 779.6 0 117 133 143 779.6 0 143 134 177 779.6 0 177 135 111 779.6 0 111 136 60 779.6 0 60 137 58 779.6 0 58 138 69 779.6 0 69 139 82 779.6 0 82 140 68 779.6 0 68 141 73 779.6 0 73 142 122 779.6 0 122 143 1933 779.6 0 1933 144 537 779.6 0 537 145 56 779.6 0 56 146 61 779.6 0 61 147 63 779.6 0 63 148 147 779.6 0 147 149 101 779.6 0 101 150 87 779.6 0 87 151 54 779.6 0 54 152 54 779.6 0 54 153 56 779.6 0 56 154 40 779.6 0 40 155 40 779.6 0 40 156 80 779.6 0 80 157 95 779.6 0 95 158 232 779.6 0 232 159 413 779.6 0 413 160 433 779.6 0 433 161 319 779.6 0 319 162 214 779.6 0 214 163 87 779.6 0 87 164 66 779.6 0 66 165 54 779.6 0 54 166 77 779.6 0 77 167 84 779.6 0 84 168 141 779.6 0 141 169 106 779.6 0 106 170 195 779.6 0 195 171 62 779.6 0 62 172 111 779.6 0 111 173 28 779.6 0 28 174 54 779.6 0 54 175 100 779.6 0 100 176 1648 779.6 0 1648 177 433 779.6 0 433 178 211 779.6 0 211 179 63 779.6 0 63 180 39 779.6 0 39 181 60 779.6 0 60 182 89 779.6 0 89 183 111 779.6 0 111 184 134 779.6 0 134 185 101 779.6 0 101 186 87 779.6 0 87 187 125 779.6 0 125 188 255 779.6 0 255 189 267 779.6 0 267 190 94 779.6 0 94 191 38 779.6 0 38 192 33 779.6 0 33 193 38 779.6 0 38 194 93 779.6 0 93 195 81 779.6 0 81 196 127 779.6 0 127 197 71 779.6 0 71 198 38 779.6 0 38 199 91 779.6 0 91 200 101 779.6 0 101 201 107 779.6 0 107 202 52 779.6 0 52 203 35 779.6 0 35 204 89 779.6 0 89 205 85 779.6 0 85 206 138 779.6 0 138 207 95 779.6 0 95 208 109 779.6 0 109 209 72 779.6 0 72 210 93 779.6 0 93 211 119 779.6 0 119 212 119 779.6 0 119 213 115 779.6 0 115 214 161 779.6 0 161 215 171 779.6 0 171 216 191 779.6 0 191 217 119 779.6 0 119 218 107 779.6 0 107 219 98 779.6 0 98 220 146 779.6 0 146 221 115 779.6 0 115 222 128 779.6 0 128 223 110 779.6 0 110 224 150 779.6 0 150 225 250 779.6 0 250 226 191 779.6 0 191 227 124 779.6 0 124 228 124 779.6 0 124 229 124 779.6 0 124 230 134 779.6 0 134 231 124 779.6 0 124 232 97 779.6 0 97 233 87 779.6 0 87 234 70 779.6 0 70 235 82 779.6 0 82 236 97 779.6 0 97 237 135 779.6 0 135 238 468 779.6 0 468 239 504 779.6 0 504 240 960 779.6 0 960 241 172 779.6 0 172 242 79 779.6 0 79 243 87 779.6 0 87 244 102 779.6 0 102 245 149 779.6 0 149 246 180 779.6 0 180 247 175 779.6 0 175 248 207 779.6 0 207 249 265 779.6 0 265 250 110 779.6 0 110 251 109 779.6 0 109 252 57 779.6 0 57 253 154 779.6 0 154 254 260 779.6 0 260 255 371 779.6 0 371 256 168 779.6 0 168 257 115 779.6 0 115 258 105 779.6 0 105 259 167 779.6 0 167 260 82 779.6 0 82 261 66 779.6 0 66 262 165 779.6 0 165 263 152 779.6 0 152 264 271 779.6 0 271 265 270 779.6 0 270 266 130 779.6 0 130 267 169 779.6 0 169 268 148 779.6 0 148 269 192 779.6 0 192 270 178 779.6 0 178 271 95 779.6 0 95 272 102 779.6 0 102 273 135 779.6 0 135 274 151 779.6 0 151 275 294 779.6 0 294 276 402 779.6 0 402 277 220 779.6 0 220 278 211 779.6 0 211 279 98 779.6 0 98 280 229 779.6 0 229 281 181 779.6 0 181 282 348 779.6 0 348 283 233 779.6 0 233 284 171 779.6 0 171 285 166 779.6 0 166 286 121 779.6 0 121 287 158 779.6 0 158 288 149 779.6 0 149 289 169 779.6 0 169 290 100 779.6 0 100 291 78 779.6 0 78 292 75 779.6 0 75 293 110 779.6 0 110 294 143 779.6 0 143 295 170 779.6 0 170 296 114 779.6 0 114 297 72 779.6 0 72 298 84 779.6 0 84 299 110 779.6 0 110 300 107 779.6 0 107 301 139 779.6 0 139 Finished in 5.30 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 106,531 Reads with adapters: 81,544 (76.5%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 81,540 (76.5%) Total basepairs processed: 29,107,238 bp Total written (filtered): 22,333,602 bp (76.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 81544 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 27 0.0 3 0 0 26 0 1 19 829 0.0 3 0 813 11 2 3 20 80111 0.0 4 78798 1259 38 12 4 21 202 0.0 4 34 167 1 22 23 0.0 4 3 3 17 23 240 0.0 4 169 0 3 62 6 24 3 0.0 4 0 0 0 0 3 29 1 0.0 4 0 1 38 1 0.0 4 1 119 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 95 0.0 4 0 0 0 0 95 130 2 0.0 4 0 0 0 0 2 153 2 0.0 4 0 0 0 0 2 189 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.42 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 81,540 Reads with adapters: 481 (0.6%) Reads written (passing filters): 81,540 (100.0%) Total basepairs processed: 22,333,602 bp Total written (filtered): 22,326,886 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 481 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.1% C: 2.1% G: 93.8% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 1274.1 0 12 4 1 318.5 0 1 9 2 0.3 0 0 2 10 3 0.1 1 0 3 11 443 0.0 1 1 442 12 12 0.0 1 1 11 152 1 0.0 1 0 1 171 1 0.0 1 1 179 1 0.0 1 0 1 191 1 0.0 1 0 1 210 1 0.0 1 0 1 222 1 0.0 1 0 1 225 1 0.0 1 1 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.