This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.53 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 107,008 (3.4%) Reads that were too short: 46,938 (1.5%) Reads written (passing filters): 60,070 (1.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 12,161,952 bp (1.3%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 107008 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 588 779.6 0 588 7 17996 779.6 0 17996 8 18 779.6 0 18 9 7 779.6 0 7 10 3 779.6 0 3 11 6 779.6 0 6 12 10 779.6 0 10 13 12 779.6 0 12 14 8 779.6 0 8 15 10 779.6 0 10 16 15 779.6 0 15 17 20 779.6 0 20 18 17 779.6 0 17 19 7 779.6 0 7 20 7 779.6 0 7 21 4 779.6 0 4 22 11 779.6 0 11 23 7 779.6 0 7 24 14 779.6 0 14 25 6 779.6 0 6 26 56 779.6 0 56 27 33 779.6 0 33 28 44 779.6 0 44 29 5 779.6 0 5 30 7 779.6 0 7 31 5 779.6 0 5 32 7 779.6 0 7 33 12 779.6 0 12 34 7 779.6 0 7 35 7 779.6 0 7 36 5 779.6 0 5 37 8 779.6 0 8 38 11 779.6 0 11 39 4 779.6 0 4 40 6 779.6 0 6 41 7 779.6 0 7 42 13 779.6 0 13 43 15 779.6 0 15 44 9 779.6 0 9 45 4 779.6 0 4 46 9 779.6 0 9 47 6 779.6 0 6 48 2 779.6 0 2 49 12 779.6 0 12 50 7 779.6 0 7 51 5 779.6 0 5 52 10 779.6 0 10 53 6 779.6 0 6 54 5 779.6 0 5 55 5 779.6 0 5 56 5 779.6 0 5 57 2 779.6 0 2 58 13 779.6 0 13 59 11 779.6 0 11 60 14 779.6 0 14 61 6 779.6 0 6 62 7 779.6 0 7 63 17 779.6 0 17 64 7 779.6 0 7 65 8 779.6 0 8 66 10 779.6 0 10 67 6 779.6 0 6 68 7 779.6 0 7 69 7 779.6 0 7 70 7 779.6 0 7 71 19 779.6 0 19 72 7 779.6 0 7 73 53 779.6 0 53 74 12 779.6 0 12 75 125 779.6 0 125 76 46 779.6 0 46 77 54 779.6 0 54 78 62 779.6 0 62 79 155 779.6 0 155 80 79 779.6 0 79 81 217 779.6 0 217 82 121 779.6 0 121 83 173 779.6 0 173 84 115 779.6 0 115 85 139 779.6 0 139 86 256 779.6 0 256 87 41 779.6 0 41 88 187 779.6 0 187 89 170 779.6 0 170 90 61 779.6 0 61 91 134 779.6 0 134 92 163 779.6 0 163 93 95 779.6 0 95 94 235 779.6 0 235 95 323 779.6 0 323 96 579 779.6 0 579 97 398 779.6 0 398 98 414 779.6 0 414 99 336 779.6 0 336 100 169 779.6 0 169 101 155 779.6 0 155 102 294 779.6 0 294 103 183 779.6 0 183 104 104 779.6 0 104 105 406 779.6 0 406 106 615 779.6 0 615 107 525 779.6 0 525 108 1897 779.6 0 1897 109 904 779.6 0 904 110 384 779.6 0 384 111 180 779.6 0 180 112 552 779.6 0 552 113 384 779.6 0 384 114 1193 779.6 0 1193 115 722 779.6 0 722 116 289 779.6 0 289 117 239 779.6 0 239 118 132 779.6 0 132 119 84 779.6 0 84 120 109 779.6 0 109 121 132 779.6 0 132 122 406 779.6 0 406 123 254 779.6 0 254 124 391 779.6 0 391 125 151 779.6 0 151 126 243 779.6 0 243 127 282 779.6 0 282 128 243 779.6 0 243 129 474 779.6 0 474 130 440 779.6 0 440 131 379 779.6 0 379 132 413 779.6 0 413 133 230 779.6 0 230 134 281 779.6 0 281 135 312 779.6 0 312 136 421 779.6 0 421 137 564 779.6 0 564 138 343 779.6 0 343 139 249 779.6 0 249 140 280 779.6 0 280 141 552 779.6 0 552 142 392 779.6 0 392 143 237 779.6 0 237 144 172 779.6 0 172 145 162 779.6 0 162 146 202 779.6 0 202 147 319 779.6 0 319 148 699 779.6 0 699 149 396 779.6 0 396 150 470 779.6 0 470 151 224 779.6 0 224 152 250 779.6 0 250 153 319 779.6 0 319 154 208 779.6 0 208 155 388 779.6 0 388 156 391 779.6 0 391 157 458 779.6 0 458 158 420 779.6 0 420 159 479 779.6 0 479 160 230 779.6 0 230 161 293 779.6 0 293 162 216 779.6 0 216 163 405 779.6 0 405 164 310 779.6 0 310 165 359 779.6 0 359 166 272 779.6 0 272 167 331 779.6 0 331 168 280 779.6 0 280 169 379 779.6 0 379 170 293 779.6 0 293 171 151 779.6 0 151 172 215 779.6 0 215 173 141 779.6 0 141 174 295 779.6 0 295 175 361 779.6 0 361 176 535 779.6 0 535 177 233 779.6 0 233 178 163 779.6 0 163 179 147 779.6 0 147 180 157 779.6 0 157 181 255 779.6 0 255 182 300 779.6 0 300 183 251 779.6 0 251 184 277 779.6 0 277 185 337 779.6 0 337 186 529 779.6 0 529 187 557 779.6 0 557 188 292 779.6 0 292 189 195 779.6 0 195 190 152 779.6 0 152 191 205 779.6 0 205 192 401 779.6 0 401 193 498 779.6 0 498 194 803 779.6 0 803 195 281 779.6 0 281 196 292 779.6 0 292 197 195 779.6 0 195 198 176 779.6 0 176 199 448 779.6 0 448 200 585 779.6 0 585 201 535 779.6 0 535 202 567 779.6 0 567 203 201 779.6 0 201 204 194 779.6 0 194 205 363 779.6 0 363 206 446 779.6 0 446 207 272 779.6 0 272 208 546 779.6 0 546 209 395 779.6 0 395 210 391 779.6 0 391 211 304 779.6 0 304 212 343 779.6 0 343 213 274 779.6 0 274 214 210 779.6 0 210 215 183 779.6 0 183 216 239 779.6 0 239 217 223 779.6 0 223 218 275 779.6 0 275 219 438 779.6 0 438 220 343 779.6 0 343 221 292 779.6 0 292 222 329 779.6 0 329 223 191 779.6 0 191 224 157 779.6 0 157 225 188 779.6 0 188 226 326 779.6 0 326 227 367 779.6 0 367 228 419 779.6 0 419 229 467 779.6 0 467 230 564 779.6 0 564 231 405 779.6 0 405 232 221 779.6 0 221 233 252 779.6 0 252 234 249 779.6 0 249 235 321 779.6 0 321 236 461 779.6 0 461 237 553 779.6 0 553 238 568 779.6 0 568 239 406 779.6 0 406 240 345 779.6 0 345 241 247 779.6 0 247 242 234 779.6 0 234 243 254 779.6 0 254 244 291 779.6 0 291 245 481 779.6 0 481 246 744 779.6 0 744 247 922 779.6 0 922 248 769 779.6 0 769 249 534 779.6 0 534 250 253 779.6 0 253 251 111 779.6 0 111 252 81 779.6 0 81 253 128 779.6 0 128 254 268 779.6 0 268 255 252 779.6 0 252 256 489 779.6 0 489 257 769 779.6 0 769 258 1461 779.6 0 1461 259 1268 779.6 0 1268 260 1245 779.6 0 1245 261 326 779.6 0 326 262 208 779.6 0 208 263 200 779.6 0 200 264 203 779.6 0 203 265 172 779.6 0 172 266 317 779.6 0 317 267 443 779.6 0 443 268 688 779.6 0 688 269 1264 779.6 0 1264 270 764 779.6 0 764 271 715 779.6 0 715 272 385 779.6 0 385 273 533 779.6 0 533 274 527 779.6 0 527 275 628 779.6 0 628 276 419 779.6 0 419 277 205 779.6 0 205 278 141 779.6 0 141 279 221 779.6 0 221 280 472 779.6 0 472 281 841 779.6 0 841 282 806 779.6 0 806 283 493 779.6 0 493 284 384 779.6 0 384 285 538 779.6 0 538 286 380 779.6 0 380 287 708 779.6 0 708 288 528 779.6 0 528 289 717 779.6 0 717 290 470 779.6 0 470 291 419 779.6 0 419 292 461 779.6 0 461 293 544 779.6 0 544 294 622 779.6 0 622 295 765 779.6 0 765 296 708 779.6 0 708 297 508 779.6 0 508 298 402 779.6 0 402 299 1197 779.6 0 1197 300 945 779.6 0 945 301 1582 779.6 0 1582 Finished in 2.51 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 60,070 Reads with adapters: 18,539 (30.9%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 18,522 (30.8%) Total basepairs processed: 12,161,952 bp Total written (filtered): 5,126,954 bp (42.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18539 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 8 2 16 259 0.0 3 0 237 9 13 17 18009 0.0 3 17468 473 55 13 18 45 0.0 3 5 39 1 19 28 0.0 3 0 2 23 3 20 145 0.0 3 120 2 0 23 21 1 0.0 3 0 0 1 30 2 0.0 3 1 0 0 1 33 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 0 4 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 18,522 Reads with adapters: 1,861 (10.0%) Reads written (passing filters): 18,522 (100.0%) Total basepairs processed: 5,126,954 bp Total written (filtered): 5,121,251 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1861 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.2% C: 57.4% G: 7.7% T: 11.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1747 289.4 0 1747 4 110 72.4 0 110 5 3 18.1 0 3 7 1 1.1 0 1