This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.27 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 139,370 (4.4%) Reads that were too short: 40,027 (1.3%) Reads written (passing filters): 99,343 (3.1%) Total basepairs processed: 961,105,341 bp Total written (filtered): 28,515,545 bp (3.0%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 139370 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 12077 779.6 0 12077 7 1495 779.6 0 1495 8 61056 779.6 0 61056 9 19922 779.6 0 19922 10 51 779.6 0 51 11 8 779.6 0 8 12 9 779.6 0 9 13 13 779.6 0 13 14 24 779.6 0 24 15 14 779.6 0 14 16 7 779.6 0 7 17 8 779.6 0 8 18 13 779.6 0 13 19 10 779.6 0 10 20 9 779.6 0 9 21 12 779.6 0 12 22 6 779.6 0 6 23 8 779.6 0 8 24 40 779.6 0 40 25 10 779.6 0 10 26 3 779.6 0 3 27 10 779.6 0 10 28 11 779.6 0 11 29 9 779.6 0 9 30 10 779.6 0 10 31 10 779.6 0 10 32 9 779.6 0 9 33 11 779.6 0 11 34 9 779.6 0 9 35 13 779.6 0 13 36 12 779.6 0 12 37 12 779.6 0 12 38 8 779.6 0 8 39 17 779.6 0 17 40 14 779.6 0 14 41 6 779.6 0 6 42 6 779.6 0 6 43 8 779.6 0 8 44 14 779.6 0 14 45 18 779.6 0 18 46 8 779.6 0 8 47 9 779.6 0 9 48 5 779.6 0 5 49 11 779.6 0 11 50 17 779.6 0 17 51 19 779.6 0 19 52 23 779.6 0 23 53 19 779.6 0 19 54 9 779.6 0 9 55 10 779.6 0 10 56 13 779.6 0 13 57 6 779.6 0 6 58 11 779.6 0 11 59 13 779.6 0 13 60 4 779.6 0 4 61 8 779.6 0 8 62 10 779.6 0 10 63 12 779.6 0 12 64 12 779.6 0 12 65 9 779.6 0 9 66 15 779.6 0 15 67 11 779.6 0 11 68 13 779.6 0 13 69 4 779.6 0 4 70 8 779.6 0 8 71 8 779.6 0 8 72 6 779.6 0 6 73 16 779.6 0 16 74 17 779.6 0 17 75 20 779.6 0 20 76 7 779.6 0 7 77 9 779.6 0 9 78 5 779.6 0 5 79 15 779.6 0 15 80 10 779.6 0 10 81 10 779.6 0 10 82 13 779.6 0 13 83 6 779.6 0 6 84 8 779.6 0 8 85 10 779.6 0 10 86 18 779.6 0 18 87 14 779.6 0 14 88 5 779.6 0 5 89 10 779.6 0 10 90 9 779.6 0 9 91 8 779.6 0 8 92 15 779.6 0 15 93 6 779.6 0 6 94 13 779.6 0 13 95 23 779.6 0 23 96 40 779.6 0 40 97 44 779.6 0 44 98 20 779.6 0 20 99 5 779.6 0 5 100 16 779.6 0 16 101 20 779.6 0 20 102 34 779.6 0 34 103 24 779.6 0 24 104 22 779.6 0 22 105 6 779.6 0 6 106 6 779.6 0 6 107 17 779.6 0 17 108 7 779.6 0 7 109 9 779.6 0 9 110 10 779.6 0 10 111 8 779.6 0 8 112 18 779.6 0 18 113 24 779.6 0 24 114 11 779.6 0 11 115 9 779.6 0 9 116 9 779.6 0 9 117 69 779.6 0 69 118 57 779.6 0 57 119 27 779.6 0 27 120 24 779.6 0 24 121 14 779.6 0 14 122 10 779.6 0 10 123 13 779.6 0 13 124 20 779.6 0 20 125 28 779.6 0 28 126 21 779.6 0 21 127 25 779.6 0 25 128 17 779.6 0 17 129 13 779.6 0 13 130 13 779.6 0 13 131 17 779.6 0 17 132 18 779.6 0 18 133 26 779.6 0 26 134 31 779.6 0 31 135 24 779.6 0 24 136 25 779.6 0 25 137 60 779.6 0 60 138 214 779.6 0 214 139 358 779.6 0 358 140 267 779.6 0 267 141 22 779.6 0 22 142 21 779.6 0 21 143 29 779.6 0 29 144 18 779.6 0 18 145 19 779.6 0 19 146 20 779.6 0 20 147 15 779.6 0 15 148 11 779.6 0 11 149 17 779.6 0 17 150 11 779.6 0 11 151 16 779.6 0 16 152 14 779.6 0 14 153 15 779.6 0 15 154 9 779.6 0 9 155 23 779.6 0 23 156 11 779.6 0 11 157 21 779.6 0 21 158 25 779.6 0 25 159 31 779.6 0 31 160 19 779.6 0 19 161 23 779.6 0 23 162 15 779.6 0 15 163 11 779.6 0 11 164 17 779.6 0 17 165 22 779.6 0 22 166 19 779.6 0 19 167 21 779.6 0 21 168 23 779.6 0 23 169 15 779.6 0 15 170 28 779.6 0 28 171 30 779.6 0 30 172 21 779.6 0 21 173 14 779.6 0 14 174 28 779.6 0 28 175 20 779.6 0 20 176 29 779.6 0 29 177 21 779.6 0 21 178 15 779.6 0 15 179 60 779.6 0 60 180 127 779.6 0 127 181 24 779.6 0 24 182 22 779.6 0 22 183 15 779.6 0 15 184 23 779.6 0 23 185 17 779.6 0 17 186 77 779.6 0 77 187 284 779.6 0 284 188 309 779.6 0 309 189 47 779.6 0 47 190 9 779.6 0 9 191 10 779.6 0 10 192 22 779.6 0 22 193 20 779.6 0 20 194 43 779.6 0 43 195 42 779.6 0 42 196 16 779.6 0 16 197 15 779.6 0 15 198 16 779.6 0 16 199 38 779.6 0 38 200 39 779.6 0 39 201 17 779.6 0 17 202 11 779.6 0 11 203 14 779.6 0 14 204 11 779.6 0 11 205 33 779.6 0 33 206 35 779.6 0 35 207 46 779.6 0 46 208 17 779.6 0 17 209 17 779.6 0 17 210 12 779.6 0 12 211 11 779.6 0 11 212 31 779.6 0 31 213 35 779.6 0 35 214 64 779.6 0 64 215 65 779.6 0 65 216 46 779.6 0 46 217 21 779.6 0 21 218 26 779.6 0 26 219 31 779.6 0 31 220 32 779.6 0 32 221 20 779.6 0 20 222 10 779.6 0 10 223 11 779.6 0 11 224 19 779.6 0 19 225 16 779.6 0 16 226 33 779.6 0 33 227 73 779.6 0 73 228 27 779.6 0 27 229 32 779.6 0 32 230 21 779.6 0 21 231 23 779.6 0 23 232 23 779.6 0 23 233 10 779.6 0 10 234 14 779.6 0 14 235 18 779.6 0 18 236 17 779.6 0 17 237 20 779.6 0 20 238 27 779.6 0 27 239 32 779.6 0 32 240 29 779.6 0 29 241 18 779.6 0 18 242 6 779.6 0 6 243 10 779.6 0 10 244 11 779.6 0 11 245 16 779.6 0 16 246 18 779.6 0 18 247 31 779.6 0 31 248 34 779.6 0 34 249 22 779.6 0 22 250 7 779.6 0 7 251 11 779.6 0 11 252 20 779.6 0 20 253 22 779.6 0 22 254 24 779.6 0 24 255 16 779.6 0 16 256 20 779.6 0 20 257 35 779.6 0 35 258 55 779.6 0 55 259 74 779.6 0 74 260 88 779.6 0 88 261 28 779.6 0 28 262 32 779.6 0 32 263 52 779.6 0 52 264 48 779.6 0 48 265 35 779.6 0 35 266 45 779.6 0 45 267 73 779.6 0 73 268 84 779.6 0 84 269 93 779.6 0 93 270 52 779.6 0 52 271 49 779.6 0 49 272 124 779.6 0 124 273 1298 779.6 0 1298 274 1079 779.6 0 1079 275 3343 779.6 0 3343 276 93 779.6 0 93 277 75 779.6 0 75 278 66 779.6 0 66 279 119 779.6 0 119 280 195 779.6 0 195 281 266 779.6 0 266 282 116 779.6 0 116 283 119 779.6 0 119 284 129 779.6 0 129 285 237 779.6 0 237 286 259 779.6 0 259 287 204 779.6 0 204 288 297 779.6 0 297 289 277 779.6 0 277 290 187 779.6 0 187 291 182 779.6 0 182 292 229 779.6 0 229 293 3670 779.6 0 3670 294 3695 779.6 0 3695 295 3605 779.6 0 3605 296 846 779.6 0 846 297 1922 779.6 0 1922 298 3759 779.6 0 3759 299 4362 779.6 0 4362 300 5387 779.6 0 5387 301 1725 779.6 0 1725 Finished in 5.97 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 99,343 Reads with adapters: 32,596 (32.8%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 32,589 (32.8%) Total basepairs processed: 28,515,545 bp Total written (filtered): 8,856,409 bp (31.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 32596 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 1 3 18 15 0.0 3 0 0 14 1 19 299 0.0 3 0 292 4 1 2 20 20541 0.0 4 20273 254 11 2 1 21 157 0.0 4 17 132 4 2 2 22 11322 0.0 4 11157 152 6 1 6 23 44 0.0 4 16 20 0 8 24 5 0.0 4 0 0 3 0 2 25 2 0.0 4 0 0 1 1 26 1 0.0 4 1 34 1 0.0 4 1 49 1 0.0 4 1 72 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 180 0.0 4 0 0 0 0 180 129 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 153 10 0.0 4 0 0 0 0 10 183 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.38 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 32,589 Reads with adapters: 2,729 (8.4%) Reads written (passing filters): 32,589 (100.0%) Total basepairs processed: 8,856,409 bp Total written (filtered): 8,245,753 bp (93.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2729 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.3% G: 18.0% T: 0.2% none/other: 81.4% Overview of removed sequences length count expect max.err error counts 4 2 127.3 0 2 5 1 31.8 0 1 6 11 8.0 0 11 9 17 0.1 0 0 17 10 122 0.0 1 3 119 11 349 0.0 1 1 348 150 1 0.0 1 0 1 158 1 0.0 1 0 1 169 1 0.0 1 1 209 2 0.0 1 0 2 222 1 0.0 1 0 1 266 1 0.0 1 1 270 3 0.0 1 3 271 5 0.0 1 5 272 2091 0.0 1 1951 140 273 98 0.0 1 89 9 274 14 0.0 1 14 275 9 0.0 1 8 1