This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.08 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 26,632 (0.8%) Reads that were too short: 8,277 (0.3%) Reads written (passing filters): 18,355 (0.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 4,171,547 bp (0.4%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 26632 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 81 779.6 0 81 7 85 779.6 0 85 8 140 779.6 0 140 9 5077 779.6 0 5077 10 33 779.6 0 33 11 20 779.6 0 20 12 15 779.6 0 15 13 16 779.6 0 16 14 14 779.6 0 14 15 20 779.6 0 20 16 16 779.6 0 16 17 100 779.6 0 100 18 53 779.6 0 53 19 64 779.6 0 64 20 12 779.6 0 12 21 42 779.6 0 42 22 19 779.6 0 19 23 26 779.6 0 26 24 7 779.6 0 7 25 13 779.6 0 13 26 9 779.6 0 9 27 12 779.6 0 12 28 18 779.6 0 18 29 14 779.6 0 14 30 13 779.6 0 13 31 11 779.6 0 11 32 13 779.6 0 13 33 21 779.6 0 21 34 14 779.6 0 14 35 11 779.6 0 11 36 16 779.6 0 16 37 16 779.6 0 16 38 18 779.6 0 18 39 11 779.6 0 11 40 23 779.6 0 23 41 16 779.6 0 16 42 22 779.6 0 22 43 32 779.6 0 32 44 21 779.6 0 21 45 20 779.6 0 20 46 55 779.6 0 55 47 943 779.6 0 943 48 344 779.6 0 344 49 1163 779.6 0 1163 50 83 779.6 0 83 51 13 779.6 0 13 52 17 779.6 0 17 53 20 779.6 0 20 54 7 779.6 0 7 55 23 779.6 0 23 56 17 779.6 0 17 57 15 779.6 0 15 58 22 779.6 0 22 59 116 779.6 0 116 60 313 779.6 0 313 61 206 779.6 0 206 62 19 779.6 0 19 63 7 779.6 0 7 64 6 779.6 0 6 65 8 779.6 0 8 66 14 779.6 0 14 67 6 779.6 0 6 68 16 779.6 0 16 69 12 779.6 0 12 70 25 779.6 0 25 71 20 779.6 0 20 72 25 779.6 0 25 73 14 779.6 0 14 74 8 779.6 0 8 75 9 779.6 0 9 76 5 779.6 0 5 77 30 779.6 0 30 78 38 779.6 0 38 79 86 779.6 0 86 80 8 779.6 0 8 81 7 779.6 0 7 82 18 779.6 0 18 83 27 779.6 0 27 84 164 779.6 0 164 85 129 779.6 0 129 86 274 779.6 0 274 87 7 779.6 0 7 88 7 779.6 0 7 89 10 779.6 0 10 90 14 779.6 0 14 91 14 779.6 0 14 92 8 779.6 0 8 93 10 779.6 0 10 94 7 779.6 0 7 95 10 779.6 0 10 96 6 779.6 0 6 97 9 779.6 0 9 98 22 779.6 0 22 99 41 779.6 0 41 100 12 779.6 0 12 101 12 779.6 0 12 102 14 779.6 0 14 103 15 779.6 0 15 104 14 779.6 0 14 105 25 779.6 0 25 106 18 779.6 0 18 107 21 779.6 0 21 108 31 779.6 0 31 109 16 779.6 0 16 110 22 779.6 0 22 111 25 779.6 0 25 112 276 779.6 0 276 113 877 779.6 0 877 114 1460 779.6 0 1460 115 11 779.6 0 11 116 13 779.6 0 13 117 13 779.6 0 13 118 15 779.6 0 15 119 12 779.6 0 12 120 7 779.6 0 7 121 10 779.6 0 10 122 6 779.6 0 6 123 17 779.6 0 17 124 23 779.6 0 23 125 16 779.6 0 16 126 16 779.6 0 16 127 39 779.6 0 39 128 61 779.6 0 61 129 66 779.6 0 66 130 29 779.6 0 29 131 46 779.6 0 46 132 119 779.6 0 119 133 98 779.6 0 98 134 150 779.6 0 150 135 71 779.6 0 71 136 31 779.6 0 31 137 18 779.6 0 18 138 30 779.6 0 30 139 29 779.6 0 29 140 52 779.6 0 52 141 1212 779.6 0 1212 142 817 779.6 0 817 143 444 779.6 0 444 144 19 779.6 0 19 145 17 779.6 0 17 146 21 779.6 0 21 147 29 779.6 0 29 148 29 779.6 0 29 149 30 779.6 0 30 150 19 779.6 0 19 151 16 779.6 0 16 152 89 779.6 0 89 153 382 779.6 0 382 154 255 779.6 0 255 155 38 779.6 0 38 156 8 779.6 0 8 157 5 779.6 0 5 158 4 779.6 0 4 159 7 779.6 0 7 160 5 779.6 0 5 161 7 779.6 0 7 162 7 779.6 0 7 163 11 779.6 0 11 164 6 779.6 0 6 165 6 779.6 0 6 166 16 779.6 0 16 167 10 779.6 0 10 168 9 779.6 0 9 169 8 779.6 0 8 170 8 779.6 0 8 171 19 779.6 0 19 172 5 779.6 0 5 173 17 779.6 0 17 174 11 779.6 0 11 175 6 779.6 0 6 176 7 779.6 0 7 177 1 779.6 0 1 178 5 779.6 0 5 179 5 779.6 0 5 180 7 779.6 0 7 181 11 779.6 0 11 182 16 779.6 0 16 183 6 779.6 0 6 184 18 779.6 0 18 185 41 779.6 0 41 186 14 779.6 0 14 187 4 779.6 0 4 188 6 779.6 0 6 189 5 779.6 0 5 190 18 779.6 0 18 191 9 779.6 0 9 192 9 779.6 0 9 193 6 779.6 0 6 194 6 779.6 0 6 195 5 779.6 0 5 196 8 779.6 0 8 197 6 779.6 0 6 198 9 779.6 0 9 199 6 779.6 0 6 200 8 779.6 0 8 201 10 779.6 0 10 202 11 779.6 0 11 203 9 779.6 0 9 204 11 779.6 0 11 205 8 779.6 0 8 206 7 779.6 0 7 207 11 779.6 0 11 208 4 779.6 0 4 209 10 779.6 0 10 210 7 779.6 0 7 211 39 779.6 0 39 212 35 779.6 0 35 213 47 779.6 0 47 214 40 779.6 0 40 215 18 779.6 0 18 216 28 779.6 0 28 217 42 779.6 0 42 218 65 779.6 0 65 219 26 779.6 0 26 220 22 779.6 0 22 221 16 779.6 0 16 222 13 779.6 0 13 223 11 779.6 0 11 224 21 779.6 0 21 225 10 779.6 0 10 226 27 779.6 0 27 227 20 779.6 0 20 228 307 779.6 0 307 229 121 779.6 0 121 230 197 779.6 0 197 231 36 779.6 0 36 232 25 779.6 0 25 233 15 779.6 0 15 234 71 779.6 0 71 235 24 779.6 0 24 236 36 779.6 0 36 237 93 779.6 0 93 238 55 779.6 0 55 239 46 779.6 0 46 240 17 779.6 0 17 241 17 779.6 0 17 242 8 779.6 0 8 243 13 779.6 0 13 244 29 779.6 0 29 245 45 779.6 0 45 246 19 779.6 0 19 247 7 779.6 0 7 248 12 779.6 0 12 249 15 779.6 0 15 250 14 779.6 0 14 251 13 779.6 0 13 252 95 779.6 0 95 253 76 779.6 0 76 254 92 779.6 0 92 255 153 779.6 0 153 256 223 779.6 0 223 257 100 779.6 0 100 258 37 779.6 0 37 259 64 779.6 0 64 260 56 779.6 0 56 261 11 779.6 0 11 262 11 779.6 0 11 263 11 779.6 0 11 264 14 779.6 0 14 265 19 779.6 0 19 266 15 779.6 0 15 267 4 779.6 0 4 268 6 779.6 0 6 269 8 779.6 0 8 270 17 779.6 0 17 271 11 779.6 0 11 272 15 779.6 0 15 273 175 779.6 0 175 274 52 779.6 0 52 275 190 779.6 0 190 276 7 779.6 0 7 277 12 779.6 0 12 278 20 779.6 0 20 279 21 779.6 0 21 280 17 779.6 0 17 281 29 779.6 0 29 282 39 779.6 0 39 283 52 779.6 0 52 284 60 779.6 0 60 285 195 779.6 0 195 286 26 779.6 0 26 287 20 779.6 0 20 288 19 779.6 0 19 289 12 779.6 0 12 290 22 779.6 0 22 291 40 779.6 0 40 292 184 779.6 0 184 293 131 779.6 0 131 294 29 779.6 0 29 295 34 779.6 0 34 296 30 779.6 0 30 297 30 779.6 0 30 298 425 779.6 0 425 299 1879 779.6 0 1879 300 1674 779.6 0 1674 301 22 779.6 0 22 Finished in 0.88 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 18,355 Reads with adapters: 5,428 (29.6%) Reads that were too short: 29 (0.2%) Reads written (passing filters): 5,399 (29.4%) Total basepairs processed: 4,171,547 bp Total written (filtered): 1,461,318 bp (35.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5428 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 1 1 16 33 0.0 3 0 28 5 17 5187 0.0 3 4969 189 18 11 18 20 0.0 3 0 11 8 1 19 3 0.0 3 1 0 0 2 20 6 0.0 3 0 0 1 5 39 3 0.0 3 0 0 0 3 42 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 50 30 0.0 3 0 0 0 30 53 8 0.0 3 0 0 0 8 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 79 20 0.0 3 0 0 0 20 82 4 0.0 3 0 0 0 4 91 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 5 0.0 3 0 0 0 5 135 3 0.0 3 0 0 0 3 139 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 30 0.0 3 0 0 0 30 144 3 0.0 3 0 0 0 3 145 3 0.0 3 0 0 0 3 147 2 0.0 3 0 0 0 2 148 4 0.0 3 0 0 0 4 152 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 181 6 0.0 3 0 0 0 6 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 246 3 0.0 3 0 0 0 3 263 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 5,399 Reads with adapters: 392 (7.3%) Reads written (passing filters): 5,399 (100.0%) Total basepairs processed: 1,461,318 bp Total written (filtered): 1,460,109 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 392 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.1% C: 6.6% G: 26.5% T: 10.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 359 84.4 0 359 4 33 21.1 0 33