This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.25 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 171,352 (5.4%) Reads that were too short: 25,240 (0.8%) Reads written (passing filters): 146,112 (4.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 41,818,295 bp (4.4%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 171352 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4205 779.6 0 4205 7 133044 779.6 0 133044 8 91 779.6 0 91 9 16 779.6 0 16 10 42 779.6 0 42 11 9 779.6 0 9 12 59 779.6 0 59 13 7 779.6 0 7 14 4 779.6 0 4 15 6 779.6 0 6 16 12 779.6 0 12 17 8 779.6 0 8 18 11 779.6 0 11 19 6 779.6 0 6 20 5 779.6 0 5 21 10 779.6 0 10 22 3 779.6 0 3 23 3 779.6 0 3 24 3 779.6 0 3 25 5 779.6 0 5 26 5 779.6 0 5 27 6 779.6 0 6 28 5 779.6 0 5 29 5 779.6 0 5 30 13 779.6 0 13 31 4 779.6 0 4 32 11 779.6 0 11 33 2 779.6 0 2 34 7 779.6 0 7 35 6 779.6 0 6 36 5 779.6 0 5 37 9 779.6 0 9 38 9 779.6 0 9 39 4 779.6 0 4 40 7 779.6 0 7 41 7 779.6 0 7 42 6 779.6 0 6 43 10 779.6 0 10 44 10 779.6 0 10 45 8 779.6 0 8 46 6 779.6 0 6 47 6 779.6 0 6 48 5 779.6 0 5 49 13 779.6 0 13 50 21 779.6 0 21 51 2 779.6 0 2 52 7 779.6 0 7 53 5 779.6 0 5 54 18 779.6 0 18 55 14 779.6 0 14 56 20 779.6 0 20 57 55 779.6 0 55 58 44 779.6 0 44 59 18 779.6 0 18 60 8 779.6 0 8 61 9 779.6 0 9 62 12 779.6 0 12 63 18 779.6 0 18 64 8 779.6 0 8 65 11 779.6 0 11 66 9 779.6 0 9 67 11 779.6 0 11 68 5 779.6 0 5 69 12 779.6 0 12 70 12 779.6 0 12 71 14 779.6 0 14 72 11 779.6 0 11 73 15 779.6 0 15 74 70 779.6 0 70 75 255 779.6 0 255 76 138 779.6 0 138 77 27 779.6 0 27 78 32 779.6 0 32 79 89 779.6 0 89 80 50 779.6 0 50 81 124 779.6 0 124 82 279 779.6 0 279 83 408 779.6 0 408 84 123 779.6 0 123 85 36 779.6 0 36 86 33 779.6 0 33 87 36 779.6 0 36 88 45 779.6 0 45 89 28 779.6 0 28 90 24 779.6 0 24 91 22 779.6 0 22 92 17 779.6 0 17 93 33 779.6 0 33 94 28 779.6 0 28 95 14 779.6 0 14 96 16 779.6 0 16 97 15 779.6 0 15 98 8 779.6 0 8 99 24 779.6 0 24 100 13 779.6 0 13 101 12 779.6 0 12 102 9 779.6 0 9 103 13 779.6 0 13 104 21 779.6 0 21 105 7 779.6 0 7 106 6 779.6 0 6 107 3 779.6 0 3 108 14 779.6 0 14 109 13 779.6 0 13 110 13 779.6 0 13 111 8 779.6 0 8 112 7 779.6 0 7 113 20 779.6 0 20 114 18 779.6 0 18 115 14 779.6 0 14 116 7 779.6 0 7 117 36 779.6 0 36 118 38 779.6 0 38 119 58 779.6 0 58 120 46 779.6 0 46 121 95 779.6 0 95 122 37 779.6 0 37 123 76 779.6 0 76 124 30 779.6 0 30 125 136 779.6 0 136 126 36 779.6 0 36 127 28 779.6 0 28 128 27 779.6 0 27 129 20 779.6 0 20 130 26 779.6 0 26 131 29 779.6 0 29 132 46 779.6 0 46 133 34 779.6 0 34 134 28 779.6 0 28 135 24 779.6 0 24 136 37 779.6 0 37 137 28 779.6 0 28 138 29 779.6 0 29 139 33 779.6 0 33 140 72 779.6 0 72 141 41 779.6 0 41 142 28 779.6 0 28 143 47 779.6 0 47 144 52 779.6 0 52 145 39 779.6 0 39 146 35 779.6 0 35 147 27 779.6 0 27 148 48 779.6 0 48 149 29 779.6 0 29 150 15 779.6 0 15 151 16 779.6 0 16 152 27 779.6 0 27 153 50 779.6 0 50 154 58 779.6 0 58 155 38 779.6 0 38 156 37 779.6 0 37 157 41 779.6 0 41 158 34 779.6 0 34 159 40 779.6 0 40 160 26 779.6 0 26 161 25 779.6 0 25 162 17 779.6 0 17 163 50 779.6 0 50 164 49 779.6 0 49 165 46 779.6 0 46 166 31 779.6 0 31 167 375 779.6 0 375 168 200 779.6 0 200 169 483 779.6 0 483 170 48 779.6 0 48 171 95 779.6 0 95 172 182 779.6 0 182 173 145 779.6 0 145 174 65 779.6 0 65 175 10 779.6 0 10 176 19 779.6 0 19 177 22 779.6 0 22 178 20 779.6 0 20 179 40 779.6 0 40 180 69 779.6 0 69 181 44 779.6 0 44 182 61 779.6 0 61 183 46 779.6 0 46 184 101 779.6 0 101 185 39 779.6 0 39 186 21 779.6 0 21 187 29 779.6 0 29 188 55 779.6 0 55 189 140 779.6 0 140 190 151 779.6 0 151 191 47 779.6 0 47 192 21 779.6 0 21 193 25 779.6 0 25 194 19 779.6 0 19 195 35 779.6 0 35 196 89 779.6 0 89 197 42 779.6 0 42 198 35 779.6 0 35 199 36 779.6 0 36 200 250 779.6 0 250 201 809 779.6 0 809 202 1440 779.6 0 1440 203 83 779.6 0 83 204 47 779.6 0 47 205 33 779.6 0 33 206 20 779.6 0 20 207 31 779.6 0 31 208 47 779.6 0 47 209 43 779.6 0 43 210 34 779.6 0 34 211 35 779.6 0 35 212 30 779.6 0 30 213 33 779.6 0 33 214 36 779.6 0 36 215 77 779.6 0 77 216 181 779.6 0 181 217 92 779.6 0 92 218 52 779.6 0 52 219 23 779.6 0 23 220 30 779.6 0 30 221 38 779.6 0 38 222 84 779.6 0 84 223 117 779.6 0 117 224 121 779.6 0 121 225 95 779.6 0 95 226 150 779.6 0 150 227 321 779.6 0 321 228 1632 779.6 0 1632 229 1927 779.6 0 1927 230 2371 779.6 0 2371 231 50 779.6 0 50 232 60 779.6 0 60 233 40 779.6 0 40 234 41 779.6 0 41 235 28 779.6 0 28 236 42 779.6 0 42 237 27 779.6 0 27 238 48 779.6 0 48 239 126 779.6 0 126 240 92 779.6 0 92 241 50 779.6 0 50 242 95 779.6 0 95 243 144 779.6 0 144 244 126 779.6 0 126 245 164 779.6 0 164 246 61 779.6 0 61 247 157 779.6 0 157 248 119 779.6 0 119 249 103 779.6 0 103 250 155 779.6 0 155 251 185 779.6 0 185 252 168 779.6 0 168 253 202 779.6 0 202 254 220 779.6 0 220 255 177 779.6 0 177 256 121 779.6 0 121 257 182 779.6 0 182 258 189 779.6 0 189 259 109 779.6 0 109 260 95 779.6 0 95 261 1032 779.6 0 1032 262 2491 779.6 0 2491 263 1817 779.6 0 1817 264 445 779.6 0 445 265 124 779.6 0 124 266 74 779.6 0 74 267 52 779.6 0 52 268 177 779.6 0 177 269 1064 779.6 0 1064 270 2010 779.6 0 2010 271 445 779.6 0 445 272 102 779.6 0 102 273 76 779.6 0 76 274 25 779.6 0 25 275 47 779.6 0 47 276 48 779.6 0 48 277 154 779.6 0 154 278 129 779.6 0 129 279 52 779.6 0 52 280 45 779.6 0 45 281 56 779.6 0 56 282 226 779.6 0 226 283 187 779.6 0 187 284 121 779.6 0 121 285 31 779.6 0 31 286 45 779.6 0 45 287 67 779.6 0 67 288 58 779.6 0 58 289 78 779.6 0 78 290 83 779.6 0 83 291 102 779.6 0 102 292 155 779.6 0 155 293 126 779.6 0 126 294 136 779.6 0 136 295 35 779.6 0 35 296 33 779.6 0 33 297 77 779.6 0 77 298 87 779.6 0 87 299 117 779.6 0 117 300 326 779.6 0 326 301 86 779.6 0 86 Finished in 6.91 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 146,112 Reads with adapters: 135,479 (92.7%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 135,469 (92.7%) Total basepairs processed: 41,818,295 bp Total written (filtered): 37,523,995 bp (89.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 135479 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 38 0.0 3 0 0 34 4 16 1372 0.0 3 0 1238 76 58 17 133294 0.0 3 127722 4594 759 219 18 297 0.0 3 57 223 14 3 19 258 0.0 3 0 1 162 95 20 171 0.0 3 63 2 3 103 21 2 0.0 3 0 1 1 22 2 0.0 3 0 0 1 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 1 27 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 1 1 172 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 187 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 284 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.44 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 135,469 Reads with adapters: 35,964 (26.5%) Reads written (passing filters): 135,469 (100.0%) Total basepairs processed: 37,523,995 bp Total written (filtered): 37,414,975 bp (99.7%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35964 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.1% C: 72.6% G: 4.1% T: 7.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 34855 2116.7 0 34855 4 1091 529.2 0 1091 5 17 132.3 0 17 6 1 33.1 0 1