This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.31 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 150,734 (4.7%) Reads that were too short: 56,252 (1.8%) Reads written (passing filters): 94,482 (3.0%) Total basepairs processed: 961,105,341 bp Total written (filtered): 19,635,425 bp (2.0%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 150734 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 7911 779.6 0 7911 7 235 779.6 0 235 8 14540 779.6 0 14540 9 33 779.6 0 33 10 10 779.6 0 10 11 3 779.6 0 3 12 11 779.6 0 11 13 8 779.6 0 8 14 5 779.6 0 5 15 7 779.6 0 7 16 9 779.6 0 9 17 4 779.6 0 4 18 7 779.6 0 7 19 8 779.6 0 8 20 7 779.6 0 7 21 7 779.6 0 7 22 18 779.6 0 18 23 6 779.6 0 6 24 3 779.6 0 3 25 18 779.6 0 18 26 7 779.6 0 7 27 9 779.6 0 9 28 8 779.6 0 8 29 9 779.6 0 9 30 4 779.6 0 4 31 12 779.6 0 12 32 13 779.6 0 13 33 42 779.6 0 42 34 5 779.6 0 5 35 6 779.6 0 6 36 11 779.6 0 11 37 7 779.6 0 7 38 6 779.6 0 6 39 17 779.6 0 17 40 10 779.6 0 10 41 8 779.6 0 8 42 39 779.6 0 39 43 7 779.6 0 7 44 11 779.6 0 11 45 6 779.6 0 6 46 11 779.6 0 11 47 14 779.6 0 14 48 4 779.6 0 4 49 14 779.6 0 14 50 7 779.6 0 7 51 26 779.6 0 26 52 77 779.6 0 77 53 60 779.6 0 60 54 2107 779.6 0 2107 55 34 779.6 0 34 56 26 779.6 0 26 57 26 779.6 0 26 58 15 779.6 0 15 59 14 779.6 0 14 60 28 779.6 0 28 61 14 779.6 0 14 62 48 779.6 0 48 63 48 779.6 0 48 64 69 779.6 0 69 65 118 779.6 0 118 66 148 779.6 0 148 67 283 779.6 0 283 68 441 779.6 0 441 69 9043 779.6 0 9043 70 4043 779.6 0 4043 71 7472 779.6 0 7472 72 44 779.6 0 44 73 14 779.6 0 14 74 17 779.6 0 17 75 69 779.6 0 69 76 40 779.6 0 40 77 17 779.6 0 17 78 76 779.6 0 76 79 75 779.6 0 75 80 196 779.6 0 196 81 296 779.6 0 296 82 252 779.6 0 252 83 86 779.6 0 86 84 195 779.6 0 195 85 367 779.6 0 367 86 151 779.6 0 151 87 185 779.6 0 185 88 1381 779.6 0 1381 89 90 779.6 0 90 90 71 779.6 0 71 91 260 779.6 0 260 92 155 779.6 0 155 93 76 779.6 0 76 94 198 779.6 0 198 95 163 779.6 0 163 96 242 779.6 0 242 97 365 779.6 0 365 98 471 779.6 0 471 99 167 779.6 0 167 100 273 779.6 0 273 101 946 779.6 0 946 102 2060 779.6 0 2060 103 185 779.6 0 185 104 227 779.6 0 227 105 157 779.6 0 157 106 46 779.6 0 46 107 120 779.6 0 120 108 417 779.6 0 417 109 181 779.6 0 181 110 36 779.6 0 36 111 86 779.6 0 86 112 145 779.6 0 145 113 163 779.6 0 163 114 238 779.6 0 238 115 111 779.6 0 111 116 130 779.6 0 130 117 216 779.6 0 216 118 252 779.6 0 252 119 190 779.6 0 190 120 405 779.6 0 405 121 200 779.6 0 200 122 224 779.6 0 224 123 247 779.6 0 247 124 223 779.6 0 223 125 171 779.6 0 171 126 259 779.6 0 259 127 176 779.6 0 176 128 238 779.6 0 238 129 331 779.6 0 331 130 232 779.6 0 232 131 280 779.6 0 280 132 446 779.6 0 446 133 784 779.6 0 784 134 583 779.6 0 583 135 242 779.6 0 242 136 392 779.6 0 392 137 728 779.6 0 728 138 730 779.6 0 730 139 396 779.6 0 396 140 337 779.6 0 337 141 184 779.6 0 184 142 242 779.6 0 242 143 354 779.6 0 354 144 219 779.6 0 219 145 175 779.6 0 175 146 201 779.6 0 201 147 368 779.6 0 368 148 476 779.6 0 476 149 336 779.6 0 336 150 355 779.6 0 355 151 291 779.6 0 291 152 304 779.6 0 304 153 222 779.6 0 222 154 223 779.6 0 223 155 260 779.6 0 260 156 402 779.6 0 402 157 420 779.6 0 420 158 679 779.6 0 679 159 449 779.6 0 449 160 479 779.6 0 479 161 365 779.6 0 365 162 415 779.6 0 415 163 299 779.6 0 299 164 292 779.6 0 292 165 252 779.6 0 252 166 245 779.6 0 245 167 297 779.6 0 297 168 421 779.6 0 421 169 472 779.6 0 472 170 295 779.6 0 295 171 461 779.6 0 461 172 429 779.6 0 429 173 252 779.6 0 252 174 167 779.6 0 167 175 582 779.6 0 582 176 1879 779.6 0 1879 177 2071 779.6 0 2071 178 1536 779.6 0 1536 179 452 779.6 0 452 180 405 779.6 0 405 181 293 779.6 0 293 182 585 779.6 0 585 183 595 779.6 0 595 184 523 779.6 0 523 185 424 779.6 0 424 186 494 779.6 0 494 187 534 779.6 0 534 188 1067 779.6 0 1067 189 1011 779.6 0 1011 190 219 779.6 0 219 191 163 779.6 0 163 192 157 779.6 0 157 193 368 779.6 0 368 194 381 779.6 0 381 195 418 779.6 0 418 196 597 779.6 0 597 197 313 779.6 0 313 198 163 779.6 0 163 199 248 779.6 0 248 200 424 779.6 0 424 201 480 779.6 0 480 202 462 779.6 0 462 203 628 779.6 0 628 204 521 779.6 0 521 205 457 779.6 0 457 206 294 779.6 0 294 207 338 779.6 0 338 208 614 779.6 0 614 209 296 779.6 0 296 210 232 779.6 0 232 211 248 779.6 0 248 212 305 779.6 0 305 213 533 779.6 0 533 214 408 779.6 0 408 215 476 779.6 0 476 216 409 779.6 0 409 217 387 779.6 0 387 218 489 779.6 0 489 219 625 779.6 0 625 220 345 779.6 0 345 221 532 779.6 0 532 222 714 779.6 0 714 223 494 779.6 0 494 224 435 779.6 0 435 225 462 779.6 0 462 226 370 779.6 0 370 227 383 779.6 0 383 228 329 779.6 0 329 229 787 779.6 0 787 230 496 779.6 0 496 231 379 779.6 0 379 232 324 779.6 0 324 233 449 779.6 0 449 234 185 779.6 0 185 235 226 779.6 0 226 236 370 779.6 0 370 237 478 779.6 0 478 238 487 779.6 0 487 239 490 779.6 0 490 240 424 779.6 0 424 241 357 779.6 0 357 242 523 779.6 0 523 243 961 779.6 0 961 244 1341 779.6 0 1341 245 592 779.6 0 592 246 388 779.6 0 388 247 346 779.6 0 346 248 464 779.6 0 464 249 456 779.6 0 456 250 458 779.6 0 458 251 414 779.6 0 414 252 657 779.6 0 657 253 1080 779.6 0 1080 254 361 779.6 0 361 255 521 779.6 0 521 256 421 779.6 0 421 257 326 779.6 0 326 258 447 779.6 0 447 259 570 779.6 0 570 260 422 779.6 0 422 261 438 779.6 0 438 262 350 779.6 0 350 263 337 779.6 0 337 264 269 779.6 0 269 265 244 779.6 0 244 266 2170 779.6 0 2170 267 2147 779.6 0 2147 268 637 779.6 0 637 269 1196 779.6 0 1196 270 983 779.6 0 983 271 1106 779.6 0 1106 272 537 779.6 0 537 273 543 779.6 0 543 274 475 779.6 0 475 275 704 779.6 0 704 276 842 779.6 0 842 277 1835 779.6 0 1835 278 911 779.6 0 911 279 434 779.6 0 434 280 379 779.6 0 379 281 606 779.6 0 606 282 565 779.6 0 565 283 694 779.6 0 694 284 892 779.6 0 892 285 465 779.6 0 465 286 277 779.6 0 277 287 359 779.6 0 359 288 458 779.6 0 458 289 645 779.6 0 645 290 453 779.6 0 453 291 511 779.6 0 511 292 482 779.6 0 482 293 442 779.6 0 442 294 610 779.6 0 610 295 711 779.6 0 711 296 541 779.6 0 541 297 392 779.6 0 392 298 335 779.6 0 335 299 389 779.6 0 389 300 370 779.6 0 370 301 1532 779.6 0 1532 Finished in 4.04 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 94,482 Reads with adapters: 14,938 (15.8%) Reads that were too short: 57 (0.1%) Reads written (passing filters): 14,881 (15.8%) Total basepairs processed: 19,635,425 bp Total written (filtered): 4,085,710 bp (20.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14938 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 4 7 16 108 0.0 3 0 94 7 7 17 10539 0.0 3 10170 325 35 9 18 4013 0.0 3 3842 148 13 10 19 77 0.0 3 2 10 53 12 20 27 0.0 3 0 2 1 24 21 4 0.0 3 0 0 0 4 22 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 34 4 0.0 3 0 0 0 4 37 5 0.0 3 0 0 0 5 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 55 5 0.0 3 0 0 0 5 57 1 0.0 3 0 0 0 1 58 12 0.0 3 0 0 0 12 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 43 0.0 3 0 0 0 43 92 2 0.0 3 0 0 0 2 94 9 0.0 3 0 0 0 9 95 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 1 2 104 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 5 0.0 3 0 0 0 5 126 1 0.0 3 0 0 0 1 130 5 0.0 3 0 0 0 5 139 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 219 4 0.0 3 0 0 0 4 220 11 0.0 3 0 0 0 11 223 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 283 4 0.0 3 0 0 0 4 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.66 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 14,881 Reads with adapters: 623 (4.2%) Reads written (passing filters): 14,881 (100.0%) Total basepairs processed: 4,085,710 bp Total written (filtered): 4,083,721 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 623 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.4% C: 13.0% G: 31.1% T: 14.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 517 232.5 0 517 4 97 58.1 0 97 5 8 14.5 0 8 10 1 0.0 1 0 1