This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 97.38 s (30 us/read; 1.97 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 249,215 (7.8%) Reads that were too short: 5,256 (0.2%) Reads written (passing filters): 243,959 (7.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 70,103,329 bp (7.3%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 249215 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1674 779.6 0 1674 7 4769 779.6 0 4769 8 4485 779.6 0 4485 9 220016 779.6 0 220016 10 300 779.6 0 300 11 21 779.6 0 21 12 7 779.6 0 7 13 18 779.6 0 18 14 10 779.6 0 10 15 12 779.6 0 12 16 23 779.6 0 23 17 10 779.6 0 10 18 14 779.6 0 14 19 23 779.6 0 23 20 46 779.6 0 46 21 34 779.6 0 34 22 32 779.6 0 32 23 367 779.6 0 367 24 521 779.6 0 521 25 762 779.6 0 762 26 13 779.6 0 13 27 9 779.6 0 9 28 12 779.6 0 12 29 18 779.6 0 18 30 8 779.6 0 8 31 12 779.6 0 12 32 12 779.6 0 12 33 13 779.6 0 13 34 12 779.6 0 12 35 26 779.6 0 26 36 27 779.6 0 27 37 20 779.6 0 20 38 7 779.6 0 7 39 8 779.6 0 8 40 9 779.6 0 9 41 11 779.6 0 11 42 7 779.6 0 7 43 8 779.6 0 8 44 15 779.6 0 15 45 13 779.6 0 13 46 5 779.6 0 5 47 12 779.6 0 12 48 5 779.6 0 5 49 6 779.6 0 6 50 12 779.6 0 12 51 14 779.6 0 14 52 17 779.6 0 17 53 112 779.6 0 112 54 16 779.6 0 16 55 28 779.6 0 28 56 16 779.6 0 16 57 11 779.6 0 11 58 10 779.6 0 10 59 13 779.6 0 13 60 10 779.6 0 10 61 6 779.6 0 6 62 9 779.6 0 9 63 10 779.6 0 10 64 6 779.6 0 6 65 30 779.6 0 30 66 11 779.6 0 11 67 8 779.6 0 8 68 8 779.6 0 8 69 65 779.6 0 65 70 16 779.6 0 16 71 50 779.6 0 50 72 28 779.6 0 28 73 41 779.6 0 41 74 70 779.6 0 70 75 326 779.6 0 326 76 13 779.6 0 13 77 13 779.6 0 13 78 20 779.6 0 20 79 13 779.6 0 13 80 12 779.6 0 12 81 23 779.6 0 23 82 20 779.6 0 20 83 24 779.6 0 24 84 25 779.6 0 25 85 33 779.6 0 33 86 30 779.6 0 30 87 31 779.6 0 31 88 690 779.6 0 690 89 495 779.6 0 495 90 336 779.6 0 336 91 4 779.6 0 4 92 10 779.6 0 10 93 6 779.6 0 6 94 13 779.6 0 13 95 29 779.6 0 29 96 78 779.6 0 78 97 66 779.6 0 66 98 54 779.6 0 54 99 29 779.6 0 29 100 43 779.6 0 43 101 231 779.6 0 231 102 254 779.6 0 254 103 388 779.6 0 388 104 23 779.6 0 23 105 20 779.6 0 20 106 23 779.6 0 23 107 9 779.6 0 9 108 9 779.6 0 9 109 11 779.6 0 11 110 21 779.6 0 21 111 29 779.6 0 29 112 34 779.6 0 34 113 84 779.6 0 84 114 122 779.6 0 122 115 21 779.6 0 21 116 25 779.6 0 25 117 43 779.6 0 43 118 37 779.6 0 37 119 23 779.6 0 23 120 40 779.6 0 40 121 17 779.6 0 17 122 43 779.6 0 43 123 35 779.6 0 35 124 31 779.6 0 31 125 50 779.6 0 50 126 121 779.6 0 121 127 1370 779.6 0 1370 128 2281 779.6 0 2281 129 1046 779.6 0 1046 130 15 779.6 0 15 131 8 779.6 0 8 132 4 779.6 0 4 133 15 779.6 0 15 134 16 779.6 0 16 135 69 779.6 0 69 136 13 779.6 0 13 137 7 779.6 0 7 138 5 779.6 0 5 139 6 779.6 0 6 140 9 779.6 0 9 141 13 779.6 0 13 142 9 779.6 0 9 143 15 779.6 0 15 144 8 779.6 0 8 145 44 779.6 0 44 146 9 779.6 0 9 147 42 779.6 0 42 148 107 779.6 0 107 149 92 779.6 0 92 150 7 779.6 0 7 151 10 779.6 0 10 152 3 779.6 0 3 153 7 779.6 0 7 154 13 779.6 0 13 155 2 779.6 0 2 156 11 779.6 0 11 157 7 779.6 0 7 158 10 779.6 0 10 159 9 779.6 0 9 160 15 779.6 0 15 161 5 779.6 0 5 162 7 779.6 0 7 163 8 779.6 0 8 164 6 779.6 0 6 165 5 779.6 0 5 166 9 779.6 0 9 167 13 779.6 0 13 168 7 779.6 0 7 169 8 779.6 0 8 170 6 779.6 0 6 171 6 779.6 0 6 172 11 779.6 0 11 173 6 779.6 0 6 174 5 779.6 0 5 175 9 779.6 0 9 176 14 779.6 0 14 177 5 779.6 0 5 178 7 779.6 0 7 179 6 779.6 0 6 180 2 779.6 0 2 181 12 779.6 0 12 182 16 779.6 0 16 183 12 779.6 0 12 184 8 779.6 0 8 185 2 779.6 0 2 186 10 779.6 0 10 187 7 779.6 0 7 188 5 779.6 0 5 189 8 779.6 0 8 190 8 779.6 0 8 191 7 779.6 0 7 192 10 779.6 0 10 193 51 779.6 0 51 194 33 779.6 0 33 195 24 779.6 0 24 196 19 779.6 0 19 197 9 779.6 0 9 198 10 779.6 0 10 199 3 779.6 0 3 200 17 779.6 0 17 201 17 779.6 0 17 202 21 779.6 0 21 203 8 779.6 0 8 204 6 779.6 0 6 205 10 779.6 0 10 206 12 779.6 0 12 207 22 779.6 0 22 208 41 779.6 0 41 209 24 779.6 0 24 210 8 779.6 0 8 211 3 779.6 0 3 212 6 779.6 0 6 213 19 779.6 0 19 214 29 779.6 0 29 215 18 779.6 0 18 216 11 779.6 0 11 217 6 779.6 0 6 218 4 779.6 0 4 219 11 779.6 0 11 220 10 779.6 0 10 221 13 779.6 0 13 222 15 779.6 0 15 223 17 779.6 0 17 224 16 779.6 0 16 225 24 779.6 0 24 226 15 779.6 0 15 227 11 779.6 0 11 228 17 779.6 0 17 229 9 779.6 0 9 230 6 779.6 0 6 231 5 779.6 0 5 232 3 779.6 0 3 233 9 779.6 0 9 234 15 779.6 0 15 235 9 779.6 0 9 236 50 779.6 0 50 237 12 779.6 0 12 238 13 779.6 0 13 239 24 779.6 0 24 240 19 779.6 0 19 241 27 779.6 0 27 242 24 779.6 0 24 243 22 779.6 0 22 244 25 779.6 0 25 245 21 779.6 0 21 246 21 779.6 0 21 247 24 779.6 0 24 248 13 779.6 0 13 249 19 779.6 0 19 250 21 779.6 0 21 251 15 779.6 0 15 252 156 779.6 0 156 253 149 779.6 0 149 254 114 779.6 0 114 255 94 779.6 0 94 256 31 779.6 0 31 257 32 779.6 0 32 258 55 779.6 0 55 259 163 779.6 0 163 260 750 779.6 0 750 261 1130 779.6 0 1130 262 619 779.6 0 619 263 10 779.6 0 10 264 11 779.6 0 11 265 10 779.6 0 10 266 28 779.6 0 28 267 44 779.6 0 44 268 8 779.6 0 8 269 9 779.6 0 9 270 2 779.6 0 2 271 3 779.6 0 3 272 3 779.6 0 3 273 12 779.6 0 12 274 16 779.6 0 16 275 16 779.6 0 16 276 16 779.6 0 16 277 18 779.6 0 18 278 12 779.6 0 12 279 149 779.6 0 149 280 108 779.6 0 108 281 43 779.6 0 43 282 12 779.6 0 12 283 6 779.6 0 6 284 7 779.6 0 7 285 17 779.6 0 17 286 8 779.6 0 8 287 21 779.6 0 21 288 83 779.6 0 83 289 24 779.6 0 24 290 8 779.6 0 8 291 14 779.6 0 14 292 21 779.6 0 21 293 20 779.6 0 20 294 17 779.6 0 17 295 18 779.6 0 18 296 11 779.6 0 11 297 30 779.6 0 30 298 55 779.6 0 55 299 111 779.6 0 111 300 85 779.6 0 85 301 74 779.6 0 74 Finished in 11.01 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 243,959 Reads with adapters: 227,290 (93.2%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 227,258 (93.2%) Total basepairs processed: 70,103,329 bp Total written (filtered): 62,495,354 bp (89.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 227290 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 100 0.0 3 0 0 79 21 16 2113 0.0 3 0 2008 77 28 17 224004 0.0 3 215889 7133 794 188 18 526 0.0 3 24 462 23 17 19 140 0.0 3 0 0 97 43 20 272 0.0 3 1 0 1 270 21 2 0.0 3 0 0 2 24 1 0.0 3 1 25 1 0.0 3 0 0 1 27 1 0.0 3 0 1 28 2 0.0 3 2 30 1 0.0 3 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 35 0.0 3 0 0 0 35 67 19 0.0 3 0 0 0 19 71 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 103 8 0.0 3 0 0 0 8 104 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 117 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 166 14 0.0 3 0 0 2 12 175 3 0.0 3 0 0 0 3 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 245 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 265 3 0.0 3 0 0 0 3 274 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 10.49 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 227,258 Reads with adapters: 20,279 (8.9%) Reads written (passing filters): 227,258 (100.0%) Total basepairs processed: 62,495,354 bp Total written (filtered): 62,432,052 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 20279 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.0% C: 7.1% G: 39.4% T: 18.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17859 3550.9 0 17859 4 2401 887.7 0 2401 5 8 221.9 0 8 6 8 55.5 0 8 7 1 13.9 0 1 13 2 0.0 1 0 2