This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 98.89 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 281,989 (8.8%) Reads that were too short: 1,662 (0.1%) Reads written (passing filters): 280,327 (8.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 81,221,233 bp (8.5%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 281989 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 14741 779.6 0 14741 7 3494 779.6 0 3494 8 38377 779.6 0 38377 9 216843 779.6 0 216843 10 490 779.6 0 490 11 39 779.6 0 39 12 9 779.6 0 9 13 13 779.6 0 13 14 7 779.6 0 7 15 17 779.6 0 17 16 11 779.6 0 11 17 6 779.6 0 6 18 6 779.6 0 6 19 5 779.6 0 5 20 4 779.6 0 4 21 9 779.6 0 9 22 3 779.6 0 3 23 4 779.6 0 4 24 6 779.6 0 6 25 5 779.6 0 5 26 14 779.6 0 14 27 13 779.6 0 13 28 15 779.6 0 15 29 15 779.6 0 15 30 7 779.6 0 7 31 9 779.6 0 9 32 5 779.6 0 5 33 4 779.6 0 4 34 9 779.6 0 9 35 23 779.6 0 23 36 7 779.6 0 7 37 12 779.6 0 12 38 5 779.6 0 5 39 6 779.6 0 6 40 11 779.6 0 11 41 10 779.6 0 10 42 35 779.6 0 35 43 95 779.6 0 95 44 9 779.6 0 9 45 10 779.6 0 10 46 17 779.6 0 17 47 18 779.6 0 18 48 155 779.6 0 155 49 183 779.6 0 183 50 309 779.6 0 309 51 8 779.6 0 8 52 7 779.6 0 7 53 6 779.6 0 6 54 4 779.6 0 4 55 5 779.6 0 5 56 7 779.6 0 7 57 8 779.6 0 8 58 6 779.6 0 6 59 5 779.6 0 5 60 2 779.6 0 2 61 8 779.6 0 8 62 14 779.6 0 14 63 107 779.6 0 107 64 31 779.6 0 31 65 9 779.6 0 9 66 5 779.6 0 5 67 5 779.6 0 5 68 10 779.6 0 10 69 4 779.6 0 4 70 4 779.6 0 4 71 7 779.6 0 7 72 3 779.6 0 3 73 1 779.6 0 1 74 5 779.6 0 5 75 10 779.6 0 10 76 5 779.6 0 5 77 10 779.6 0 10 78 5 779.6 0 5 79 3 779.6 0 3 80 7 779.6 0 7 81 14 779.6 0 14 82 23 779.6 0 23 83 586 779.6 0 586 84 23 779.6 0 23 85 29 779.6 0 29 86 13 779.6 0 13 87 7 779.6 0 7 88 3 779.6 0 3 89 14 779.6 0 14 90 3 779.6 0 3 91 5 779.6 0 5 92 3 779.6 0 3 93 3 779.6 0 3 94 5 779.6 0 5 95 15 779.6 0 15 96 225 779.6 0 225 97 7 779.6 0 7 98 8 779.6 0 8 99 5 779.6 0 5 100 2 779.6 0 2 101 3 779.6 0 3 102 4 779.6 0 4 103 3 779.6 0 3 104 4 779.6 0 4 105 7 779.6 0 7 106 5 779.6 0 5 107 3 779.6 0 3 108 3 779.6 0 3 109 9 779.6 0 9 110 3 779.6 0 3 111 5 779.6 0 5 112 8 779.6 0 8 113 8 779.6 0 8 114 9 779.6 0 9 115 1 779.6 0 1 116 1 779.6 0 1 117 4 779.6 0 4 118 26 779.6 0 26 119 18 779.6 0 18 120 28 779.6 0 28 121 3 779.6 0 3 122 11 779.6 0 11 123 7 779.6 0 7 124 2 779.6 0 2 125 7 779.6 0 7 126 6 779.6 0 6 127 12 779.6 0 12 128 7 779.6 0 7 129 7 779.6 0 7 130 9 779.6 0 9 131 10 779.6 0 10 132 11 779.6 0 11 133 25 779.6 0 25 134 8 779.6 0 8 135 15 779.6 0 15 136 17 779.6 0 17 137 9 779.6 0 9 138 11 779.6 0 11 139 8 779.6 0 8 140 12 779.6 0 12 141 15 779.6 0 15 142 12 779.6 0 12 143 17 779.6 0 17 144 29 779.6 0 29 145 22 779.6 0 22 146 73 779.6 0 73 147 720 779.6 0 720 148 1343 779.6 0 1343 149 18 779.6 0 18 150 7 779.6 0 7 151 10 779.6 0 10 152 9 779.6 0 9 153 69 779.6 0 69 154 8 779.6 0 8 155 8 779.6 0 8 156 5 779.6 0 5 157 2 779.6 0 2 158 3 779.6 0 3 159 4 779.6 0 4 160 6 779.6 0 6 161 7 779.6 0 7 162 1 779.6 0 1 163 4 779.6 0 4 164 2 779.6 0 2 165 2 779.6 0 2 166 6 779.6 0 6 167 3 779.6 0 3 168 7 779.6 0 7 169 7 779.6 0 7 170 4 779.6 0 4 171 14 779.6 0 14 172 2 779.6 0 2 173 2 779.6 0 2 174 2 779.6 0 2 175 4 779.6 0 4 176 3 779.6 0 3 177 5 779.6 0 5 178 14 779.6 0 14 179 8 779.6 0 8 180 9 779.6 0 9 181 2 779.6 0 2 182 19 779.6 0 19 183 9 779.6 0 9 184 5 779.6 0 5 185 10 779.6 0 10 186 10 779.6 0 10 187 9 779.6 0 9 188 11 779.6 0 11 189 7 779.6 0 7 190 9 779.6 0 9 191 208 779.6 0 208 192 60 779.6 0 60 193 49 779.6 0 49 194 29 779.6 0 29 195 128 779.6 0 128 196 364 779.6 0 364 197 162 779.6 0 162 198 6 779.6 0 6 199 2 779.6 0 2 200 3 779.6 0 3 201 1 779.6 0 1 202 6 779.6 0 6 203 3 779.6 0 3 204 2 779.6 0 2 205 3 779.6 0 3 206 4 779.6 0 4 207 3 779.6 0 3 208 5 779.6 0 5 209 4 779.6 0 4 210 10 779.6 0 10 211 1 779.6 0 1 212 5 779.6 0 5 213 8 779.6 0 8 214 16 779.6 0 16 215 11 779.6 0 11 216 15 779.6 0 15 217 15 779.6 0 15 218 19 779.6 0 19 219 17 779.6 0 17 220 7 779.6 0 7 221 12 779.6 0 12 222 2 779.6 0 2 223 4 779.6 0 4 224 6 779.6 0 6 225 10 779.6 0 10 226 9 779.6 0 9 227 10 779.6 0 10 228 5 779.6 0 5 229 7 779.6 0 7 230 9 779.6 0 9 231 10 779.6 0 10 232 7 779.6 0 7 233 9 779.6 0 9 234 184 779.6 0 184 235 25 779.6 0 25 236 71 779.6 0 71 237 26 779.6 0 26 238 12 779.6 0 12 239 26 779.6 0 26 240 9 779.6 0 9 241 6 779.6 0 6 242 3 779.6 0 3 243 8 779.6 0 8 244 26 779.6 0 26 245 32 779.6 0 32 246 22 779.6 0 22 247 8 779.6 0 8 248 3 779.6 0 3 249 3 779.6 0 3 250 3 779.6 0 3 251 5 779.6 0 5 252 18 779.6 0 18 253 112 779.6 0 112 254 10 779.6 0 10 255 28 779.6 0 28 256 27 779.6 0 27 257 27 779.6 0 27 258 39 779.6 0 39 259 26 779.6 0 26 260 34 779.6 0 34 261 13 779.6 0 13 262 15 779.6 0 15 263 6 779.6 0 6 264 3 779.6 0 3 265 5 779.6 0 5 266 4 779.6 0 4 267 5 779.6 0 5 268 10 779.6 0 10 269 19 779.6 0 19 270 10 779.6 0 10 271 5 779.6 0 5 272 2 779.6 0 2 273 1 779.6 0 1 274 11 779.6 0 11 275 2 779.6 0 2 276 6 779.6 0 6 277 4 779.6 0 4 278 6 779.6 0 6 279 15 779.6 0 15 280 8 779.6 0 8 281 13 779.6 0 13 282 9 779.6 0 9 283 8 779.6 0 8 284 25 779.6 0 25 285 27 779.6 0 27 286 14 779.6 0 14 287 23 779.6 0 23 288 12 779.6 0 12 289 79 779.6 0 79 290 8 779.6 0 8 291 12 779.6 0 12 292 45 779.6 0 45 293 54 779.6 0 54 294 13 779.6 0 13 295 6 779.6 0 6 296 27 779.6 0 27 297 19 779.6 0 19 298 14 779.6 0 14 299 18 779.6 0 18 300 18 779.6 0 18 301 21 779.6 0 21 Finished in 13.76 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 280,327 Reads with adapters: 236,349 (84.3%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 236,346 (84.3%) Total basepairs processed: 81,221,233 bp Total written (filtered): 64,297,367 bp (79.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 236349 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 5 0.0 3 0 0 0 3 2 18 159 0.0 3 0 0 151 7 1 19 3656 0.0 3 0 3597 41 7 11 20 231098 0.0 4 227704 3283 89 15 7 21 547 0.0 4 107 435 4 1 22 641 0.0 4 574 14 50 2 1 23 81 0.0 4 1 1 0 71 8 24 5 0.0 4 0 0 0 1 4 25 3 0.0 4 0 0 0 1 2 26 2 0.0 4 1 0 1 28 2 0.0 4 0 1 0 0 1 29 1 0.0 4 1 31 1 0.0 4 1 33 1 0.0 4 1 34 1 0.0 4 1 36 1 0.0 4 1 41 2 0.0 4 2 86 4 0.0 4 0 0 0 0 4 126 2 0.0 4 0 0 0 0 2 127 103 0.0 4 0 0 0 0 103 130 1 0.0 4 0 0 0 0 1 135 5 0.0 4 0 0 0 0 5 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 155 18 0.0 4 0 0 0 0 18 237 1 0.0 4 1 260 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.97 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 236,346 Reads with adapters: 3,768 (1.6%) Reads written (passing filters): 236,346 (100.0%) Total basepairs processed: 64,297,367 bp Total written (filtered): 64,255,164 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3768 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 2.1% G: 94.8% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 61 3692.9 0 61 4 34 923.2 0 34 5 10 230.8 0 10 6 7 57.7 0 7 7 1 14.4 0 1 8 2 3.6 0 2 9 262 0.9 0 5 257 10 1429 0.2 1 42 1387 11 1934 0.1 1 4 1930 12 1 0.0 1 0 1 13 3 0.0 1 0 3 16 1 0.0 1 1 62 1 0.0 1 1 70 2 0.0 1 1 1 150 9 0.0 1 0 9 151 1 0.0 1 0 1 169 1 0.0 1 1 170 1 0.0 1 0 1 189 2 0.0 1 0 2 191 1 0.0 1 1 208 2 0.0 1 1 1 223 1 0.0 1 1 258 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.