This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.96 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 44,522 (1.4%) Reads that were too short: 16,510 (0.5%) Reads written (passing filters): 28,012 (0.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 6,336,349 bp (0.7%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 44522 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 95 779.6 0 95 7 193 779.6 0 193 8 9774 779.6 0 9774 9 131 779.6 0 131 10 124 779.6 0 124 11 3 779.6 0 3 13 2 779.6 0 2 14 11 779.6 0 11 15 14 779.6 0 14 16 7 779.6 0 7 17 12 779.6 0 12 18 11 779.6 0 11 19 17 779.6 0 17 20 7 779.6 0 7 21 5 779.6 0 5 22 7 779.6 0 7 23 5 779.6 0 5 24 5 779.6 0 5 25 4 779.6 0 4 26 11 779.6 0 11 27 33 779.6 0 33 28 75 779.6 0 75 29 401 779.6 0 401 30 34 779.6 0 34 31 143 779.6 0 143 32 387 779.6 0 387 33 10 779.6 0 10 34 3 779.6 0 3 35 7 779.6 0 7 36 8 779.6 0 8 37 6 779.6 0 6 38 4 779.6 0 4 39 11 779.6 0 11 40 11 779.6 0 11 41 8 779.6 0 8 42 8 779.6 0 8 43 14 779.6 0 14 44 22 779.6 0 22 45 175 779.6 0 175 46 323 779.6 0 323 47 214 779.6 0 214 48 6 779.6 0 6 49 4 779.6 0 4 50 8 779.6 0 8 51 15 779.6 0 15 52 5 779.6 0 5 53 4 779.6 0 4 54 5 779.6 0 5 55 4 779.6 0 4 56 5 779.6 0 5 57 3 779.6 0 3 58 4 779.6 0 4 59 2 779.6 0 2 60 7 779.6 0 7 61 6 779.6 0 6 62 6 779.6 0 6 63 8 779.6 0 8 64 3 779.6 0 3 65 5 779.6 0 5 66 14 779.6 0 14 67 10 779.6 0 10 68 73 779.6 0 73 69 39 779.6 0 39 70 492 779.6 0 492 71 110 779.6 0 110 72 80 779.6 0 80 73 13 779.6 0 13 74 26 779.6 0 26 75 25 779.6 0 25 76 63 779.6 0 63 77 227 779.6 0 227 78 108 779.6 0 108 79 94 779.6 0 94 80 281 779.6 0 281 81 151 779.6 0 151 82 140 779.6 0 140 83 328 779.6 0 328 84 340 779.6 0 340 85 54 779.6 0 54 86 28 779.6 0 28 87 72 779.6 0 72 88 19 779.6 0 19 89 21 779.6 0 21 90 197 779.6 0 197 91 80 779.6 0 80 92 196 779.6 0 196 93 502 779.6 0 502 94 112 779.6 0 112 95 206 779.6 0 206 96 191 779.6 0 191 97 883 779.6 0 883 98 66 779.6 0 66 99 47 779.6 0 47 100 91 779.6 0 91 101 31 779.6 0 31 102 11 779.6 0 11 103 13 779.6 0 13 104 27 779.6 0 27 105 11 779.6 0 11 106 28 779.6 0 28 107 36 779.6 0 36 108 11 779.6 0 11 109 38 779.6 0 38 110 98 779.6 0 98 111 52 779.6 0 52 112 58 779.6 0 58 113 299 779.6 0 299 114 590 779.6 0 590 115 1032 779.6 0 1032 116 191 779.6 0 191 117 59 779.6 0 59 118 112 779.6 0 112 119 83 779.6 0 83 120 49 779.6 0 49 121 64 779.6 0 64 122 48 779.6 0 48 123 74 779.6 0 74 124 91 779.6 0 91 125 106 779.6 0 106 126 43 779.6 0 43 127 26 779.6 0 26 128 236 779.6 0 236 129 170 779.6 0 170 130 167 779.6 0 167 131 170 779.6 0 170 132 83 779.6 0 83 133 75 779.6 0 75 134 111 779.6 0 111 135 275 779.6 0 275 136 179 779.6 0 179 137 287 779.6 0 287 138 188 779.6 0 188 139 238 779.6 0 238 140 266 779.6 0 266 141 65 779.6 0 65 142 91 779.6 0 91 143 116 779.6 0 116 144 151 779.6 0 151 145 71 779.6 0 71 146 48 779.6 0 48 147 39 779.6 0 39 148 40 779.6 0 40 149 38 779.6 0 38 150 46 779.6 0 46 151 60 779.6 0 60 152 73 779.6 0 73 153 41 779.6 0 41 154 74 779.6 0 74 155 73 779.6 0 73 156 51 779.6 0 51 157 75 779.6 0 75 158 59 779.6 0 59 159 51 779.6 0 51 160 40 779.6 0 40 161 44 779.6 0 44 162 86 779.6 0 86 163 97 779.6 0 97 164 110 779.6 0 110 165 98 779.6 0 98 166 80 779.6 0 80 167 106 779.6 0 106 168 164 779.6 0 164 169 123 779.6 0 123 170 56 779.6 0 56 171 65 779.6 0 65 172 48 779.6 0 48 173 62 779.6 0 62 174 35 779.6 0 35 175 32 779.6 0 32 176 24 779.6 0 24 177 73 779.6 0 73 178 126 779.6 0 126 179 131 779.6 0 131 180 78 779.6 0 78 181 85 779.6 0 85 182 64 779.6 0 64 183 76 779.6 0 76 184 124 779.6 0 124 185 91 779.6 0 91 186 46 779.6 0 46 187 42 779.6 0 42 188 33 779.6 0 33 189 106 779.6 0 106 190 157 779.6 0 157 191 132 779.6 0 132 192 92 779.6 0 92 193 43 779.6 0 43 194 23 779.6 0 23 195 67 779.6 0 67 196 55 779.6 0 55 197 59 779.6 0 59 198 81 779.6 0 81 199 57 779.6 0 57 200 32 779.6 0 32 201 67 779.6 0 67 202 236 779.6 0 236 203 406 779.6 0 406 204 740 779.6 0 740 205 134 779.6 0 134 206 73 779.6 0 73 207 63 779.6 0 63 208 73 779.6 0 73 209 175 779.6 0 175 210 315 779.6 0 315 211 174 779.6 0 174 212 147 779.6 0 147 213 80 779.6 0 80 214 103 779.6 0 103 215 197 779.6 0 197 216 132 779.6 0 132 217 114 779.6 0 114 218 51 779.6 0 51 219 39 779.6 0 39 220 79 779.6 0 79 221 110 779.6 0 110 222 79 779.6 0 79 223 103 779.6 0 103 224 154 779.6 0 154 225 497 779.6 0 497 226 627 779.6 0 627 227 775 779.6 0 775 228 188 779.6 0 188 229 34 779.6 0 34 230 44 779.6 0 44 231 101 779.6 0 101 232 178 779.6 0 178 233 174 779.6 0 174 234 228 779.6 0 228 235 917 779.6 0 917 236 744 779.6 0 744 237 712 779.6 0 712 238 212 779.6 0 212 239 42 779.6 0 42 240 57 779.6 0 57 241 98 779.6 0 98 242 131 779.6 0 131 243 74 779.6 0 74 244 46 779.6 0 46 245 21 779.6 0 21 246 23 779.6 0 23 247 20 779.6 0 20 248 37 779.6 0 37 249 53 779.6 0 53 250 91 779.6 0 91 251 97 779.6 0 97 252 212 779.6 0 212 253 221 779.6 0 221 254 57 779.6 0 57 255 22 779.6 0 22 256 9 779.6 0 9 257 23 779.6 0 23 258 31 779.6 0 31 259 75 779.6 0 75 260 64 779.6 0 64 261 136 779.6 0 136 262 140 779.6 0 140 263 218 779.6 0 218 264 95 779.6 0 95 265 115 779.6 0 115 266 40 779.6 0 40 267 21 779.6 0 21 268 33 779.6 0 33 269 79 779.6 0 79 270 295 779.6 0 295 271 156 779.6 0 156 272 79 779.6 0 79 273 64 779.6 0 64 274 127 779.6 0 127 275 110 779.6 0 110 276 60 779.6 0 60 277 88 779.6 0 88 278 77 779.6 0 77 279 53 779.6 0 53 280 48 779.6 0 48 281 69 779.6 0 69 282 96 779.6 0 96 283 62 779.6 0 62 284 61 779.6 0 61 285 55 779.6 0 55 286 51 779.6 0 51 287 54 779.6 0 54 288 89 779.6 0 89 289 285 779.6 0 285 290 217 779.6 0 217 291 234 779.6 0 234 292 97 779.6 0 97 293 142 779.6 0 142 294 163 779.6 0 163 295 300 779.6 0 300 296 566 779.6 0 566 297 316 779.6 0 316 298 122 779.6 0 122 299 283 779.6 0 283 300 251 779.6 0 251 301 251 779.6 0 251 Finished in 1.31 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 28,012 Reads with adapters: 10,061 (35.9%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 10,050 (35.9%) Total basepairs processed: 6,336,349 bp Total written (filtered): 2,764,376 bp (43.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10061 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 1 2 16 115 0.0 3 0 104 3 8 17 9772 0.0 3 9503 240 25 4 18 32 0.0 3 4 22 3 3 19 50 0.0 3 2 1 38 9 20 20 0.0 3 1 1 0 18 24 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 54 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 78 15 0.0 3 0 0 0 15 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 6 0.0 3 0 0 0 6 84 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 1 99 2 0.0 3 0 0 0 2 113 6 0.0 3 0 0 0 6 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 163 6 0.0 3 0 0 0 6 165 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 183 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 10,050 Reads with adapters: 218 (2.2%) Reads written (passing filters): 10,050 (100.0%) Total basepairs processed: 2,764,376 bp Total written (filtered): 2,763,648 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 218 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.8% C: 29.8% G: 13.3% T: 32.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 160 157.0 0 160 4 42 39.3 0 42 5 16 9.8 0 16