This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.27 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 525,496 (16.5%) Reads that were too short: 50,666 (1.6%) Reads written (passing filters): 474,830 (14.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 81,411,488 bp (8.5%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 525496 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 380 779.6 0 380 7 25594 779.6 0 25594 8 63 779.6 0 63 9 91 779.6 0 91 10 72 779.6 0 72 11 42 779.6 0 42 12 31 779.6 0 31 13 34 779.6 0 34 14 31 779.6 0 31 15 46 779.6 0 46 16 41 779.6 0 41 17 38 779.6 0 38 18 243 779.6 0 243 19 173 779.6 0 173 20 218 779.6 0 218 21 28 779.6 0 28 22 33 779.6 0 33 23 40 779.6 0 40 24 44 779.6 0 44 25 56 779.6 0 56 26 52 779.6 0 52 27 116 779.6 0 116 28 38 779.6 0 38 29 27 779.6 0 27 30 27 779.6 0 27 31 17 779.6 0 17 32 24 779.6 0 24 33 33 779.6 0 33 34 34 779.6 0 34 35 38 779.6 0 38 36 32 779.6 0 32 37 45 779.6 0 45 38 37 779.6 0 37 39 30 779.6 0 30 40 62 779.6 0 62 41 30 779.6 0 30 42 28 779.6 0 28 43 27 779.6 0 27 44 23 779.6 0 23 45 21 779.6 0 21 46 34 779.6 0 34 47 29 779.6 0 29 48 24 779.6 0 24 49 38 779.6 0 38 50 17 779.6 0 17 51 27 779.6 0 27 52 33 779.6 0 33 53 87 779.6 0 87 54 44 779.6 0 44 55 66 779.6 0 66 56 590 779.6 0 590 57 55 779.6 0 55 58 39 779.6 0 39 59 29 779.6 0 29 60 30 779.6 0 30 61 33 779.6 0 33 62 50 779.6 0 50 63 56 779.6 0 56 64 49 779.6 0 49 65 35 779.6 0 35 66 28 779.6 0 28 67 66 779.6 0 66 68 64 779.6 0 64 69 100 779.6 0 100 70 159 779.6 0 159 71 70 779.6 0 70 72 297 779.6 0 297 73 175 779.6 0 175 74 335 779.6 0 335 75 1142 779.6 0 1142 76 173 779.6 0 173 77 458 779.6 0 458 78 860 779.6 0 860 79 847 779.6 0 847 80 1276 779.6 0 1276 81 1953 779.6 0 1953 82 1064 779.6 0 1064 83 3109 779.6 0 3109 84 3153 779.6 0 3153 85 6107 779.6 0 6107 86 3889 779.6 0 3889 87 1181 779.6 0 1181 88 2429 779.6 0 2429 89 209 779.6 0 209 90 778 779.6 0 778 91 1342 779.6 0 1342 92 399 779.6 0 399 93 957 779.6 0 957 94 985 779.6 0 985 95 662 779.6 0 662 96 464 779.6 0 464 97 496 779.6 0 496 98 773 779.6 0 773 99 387 779.6 0 387 100 379 779.6 0 379 101 708 779.6 0 708 102 602 779.6 0 602 103 423 779.6 0 423 104 708 779.6 0 708 105 1231 779.6 0 1231 106 269 779.6 0 269 107 482 779.6 0 482 108 1080 779.6 0 1080 109 396 779.6 0 396 110 439 779.6 0 439 111 946 779.6 0 946 112 669 779.6 0 669 113 924 779.6 0 924 114 1302 779.6 0 1302 115 912 779.6 0 912 116 1349 779.6 0 1349 117 2425 779.6 0 2425 118 16718 779.6 0 16718 119 14460 779.6 0 14460 120 30651 779.6 0 30651 121 3196 779.6 0 3196 122 2365 779.6 0 2365 123 4711 779.6 0 4711 124 815 779.6 0 815 125 767 779.6 0 767 126 1151 779.6 0 1151 127 922 779.6 0 922 128 781 779.6 0 781 129 1275 779.6 0 1275 130 937 779.6 0 937 131 1286 779.6 0 1286 132 2318 779.6 0 2318 133 1202 779.6 0 1202 134 1387 779.6 0 1387 135 1440 779.6 0 1440 136 1364 779.6 0 1364 137 1251 779.6 0 1251 138 1893 779.6 0 1893 139 6887 779.6 0 6887 140 13772 779.6 0 13772 141 19028 779.6 0 19028 142 19763 779.6 0 19763 143 3986 779.6 0 3986 144 4859 779.6 0 4859 145 70822 779.6 0 70822 146 48612 779.6 0 48612 147 47207 779.6 0 47207 148 7187 779.6 0 7187 149 2789 779.6 0 2789 150 2102 779.6 0 2102 151 1352 779.6 0 1352 152 1105 779.6 0 1105 153 820 779.6 0 820 154 793 779.6 0 793 155 1374 779.6 0 1374 156 1092 779.6 0 1092 157 1380 779.6 0 1380 158 1075 779.6 0 1075 159 804 779.6 0 804 160 623 779.6 0 623 161 765 779.6 0 765 162 835 779.6 0 835 163 738 779.6 0 738 164 1090 779.6 0 1090 165 4060 779.6 0 4060 166 3710 779.6 0 3710 167 12783 779.6 0 12783 168 1001 779.6 0 1001 169 773 779.6 0 773 170 433 779.6 0 433 171 626 779.6 0 626 172 719 779.6 0 719 173 484 779.6 0 484 174 570 779.6 0 570 175 630 779.6 0 630 176 632 779.6 0 632 177 470 779.6 0 470 178 594 779.6 0 594 179 402 779.6 0 402 180 512 779.6 0 512 181 477 779.6 0 477 182 479 779.6 0 479 183 517 779.6 0 517 184 435 779.6 0 435 185 639 779.6 0 639 186 533 779.6 0 533 187 576 779.6 0 576 188 570 779.6 0 570 189 605 779.6 0 605 190 618 779.6 0 618 191 494 779.6 0 494 192 584 779.6 0 584 193 1268 779.6 0 1268 194 886 779.6 0 886 195 585 779.6 0 585 196 902 779.6 0 902 197 570 779.6 0 570 198 661 779.6 0 661 199 682 779.6 0 682 200 855 779.6 0 855 201 710 779.6 0 710 202 479 779.6 0 479 203 491 779.6 0 491 204 504 779.6 0 504 205 596 779.6 0 596 206 480 779.6 0 480 207 411 779.6 0 411 208 494 779.6 0 494 209 519 779.6 0 519 210 642 779.6 0 642 211 375 779.6 0 375 212 496 779.6 0 496 213 619 779.6 0 619 214 390 779.6 0 390 215 470 779.6 0 470 216 498 779.6 0 498 217 543 779.6 0 543 218 725 779.6 0 725 219 831 779.6 0 831 220 443 779.6 0 443 221 547 779.6 0 547 222 408 779.6 0 408 223 330 779.6 0 330 224 335 779.6 0 335 225 265 779.6 0 265 226 296 779.6 0 296 227 316 779.6 0 316 228 301 779.6 0 301 229 434 779.6 0 434 230 412 779.6 0 412 231 396 779.6 0 396 232 569 779.6 0 569 233 566 779.6 0 566 234 425 779.6 0 425 235 442 779.6 0 442 236 565 779.6 0 565 237 507 779.6 0 507 238 609 779.6 0 609 239 754 779.6 0 754 240 672 779.6 0 672 241 535 779.6 0 535 242 473 779.6 0 473 243 267 779.6 0 267 244 381 779.6 0 381 245 365 779.6 0 365 246 589 779.6 0 589 247 959 779.6 0 959 248 1684 779.6 0 1684 249 658 779.6 0 658 250 450 779.6 0 450 251 417 779.6 0 417 252 232 779.6 0 232 253 194 779.6 0 194 254 159 779.6 0 159 255 129 779.6 0 129 256 193 779.6 0 193 257 317 779.6 0 317 258 322 779.6 0 322 259 444 779.6 0 444 260 437 779.6 0 437 261 488 779.6 0 488 262 447 779.6 0 447 263 453 779.6 0 453 264 332 779.6 0 332 265 226 779.6 0 226 266 295 779.6 0 295 267 253 779.6 0 253 268 327 779.6 0 327 269 351 779.6 0 351 270 413 779.6 0 413 271 484 779.6 0 484 272 649 779.6 0 649 273 815 779.6 0 815 274 1015 779.6 0 1015 275 555 779.6 0 555 276 428 779.6 0 428 277 374 779.6 0 374 278 388 779.6 0 388 279 434 779.6 0 434 280 384 779.6 0 384 281 269 779.6 0 269 282 327 779.6 0 327 283 303 779.6 0 303 284 395 779.6 0 395 285 307 779.6 0 307 286 303 779.6 0 303 287 498 779.6 0 498 288 496 779.6 0 496 289 531 779.6 0 531 290 522 779.6 0 522 291 567 779.6 0 567 292 538 779.6 0 538 293 423 779.6 0 423 294 535 779.6 0 535 295 580 779.6 0 580 296 644 779.6 0 644 297 1209 779.6 0 1209 298 987 779.6 0 987 299 1204 779.6 0 1204 300 1183 779.6 0 1183 301 1374 779.6 0 1374 Finished in 17.95 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 474,830 Reads with adapters: 30,124 (6.3%) Reads that were too short: 688 (0.1%) Reads written (passing filters): 29,436 (6.2%) Total basepairs processed: 81,411,488 bp Total written (filtered): 7,569,672 bp (9.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30124 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 6 1 16 222 0.0 3 0 195 16 11 17 25430 0.0 3 24465 780 150 35 18 51 0.0 3 5 39 3 4 19 58 0.0 3 5 4 22 27 20 18 0.0 3 0 0 0 18 21 1 0.0 3 0 0 0 1 22 4 0.0 3 0 0 0 4 23 20 0.0 3 0 0 0 20 24 1 0.0 3 0 0 0 1 25 3 0.0 3 0 0 0 3 26 1033 0.0 3 0 0 2 1031 27 6 0.0 3 0 1 0 5 29 158 0.0 3 0 0 2 156 32 3 0.0 3 0 0 1 2 34 1 0.0 3 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 46 6 0.0 3 0 0 0 6 47 3 0.0 3 0 0 0 3 48 3 0.0 3 0 0 0 3 49 5 0.0 3 0 0 0 5 50 42 0.0 3 0 0 1 41 51 2 0.0 3 0 0 0 2 52 7 0.0 3 0 0 0 7 53 75 0.0 3 0 0 0 75 54 10 0.0 3 0 0 0 10 56 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 4 0.0 3 0 0 0 4 72 1791 0.0 3 0 0 10 1781 73 46 0.0 3 0 0 0 46 74 245 0.0 3 0 0 0 245 75 2 0.0 3 0 0 0 2 76 33 0.0 3 0 0 1 32 77 53 0.0 3 0 0 0 53 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 6 0.0 3 0 0 0 6 87 3 0.0 3 0 0 1 2 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 1 97 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 14 0.0 3 0 0 0 14 107 28 0.0 3 0 0 0 28 108 4 0.0 3 0 0 0 4 109 2 0.0 3 0 0 0 2 110 37 0.0 3 0 0 1 36 111 5 0.0 3 0 0 1 4 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 60 0.0 3 0 0 3 57 129 14 0.0 3 0 0 0 14 133 4 0.0 3 0 0 0 4 134 2 0.0 3 0 0 0 2 135 5 0.0 3 0 0 0 5 136 1 0.0 3 0 0 0 1 138 198 0.0 3 0 0 1 197 139 23 0.0 3 0 0 0 23 140 24 0.0 3 0 0 0 24 141 152 0.0 3 0 0 4 148 142 7 0.0 3 0 0 0 7 144 2 0.0 3 0 0 0 2 146 21 0.0 3 0 0 0 21 147 41 0.0 3 0 0 1 40 148 43 0.0 3 0 0 4 39 149 1 0.0 3 0 0 1 150 2 0.0 3 0 0 0 2 151 6 0.0 3 0 0 0 6 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 5 0.0 3 0 0 0 5 160 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 175 3 0.0 3 0 0 0 3 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 198 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 209 8 0.0 3 0 0 1 7 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.34 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 29,436 Reads with adapters: 4,807 (16.3%) Reads written (passing filters): 29,436 (100.0%) Total basepairs processed: 7,569,672 bp Total written (filtered): 7,554,997 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4807 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.7% C: 58.8% G: 12.6% T: 10.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4556 459.9 0 4556 4 249 115.0 0 249 5 1 28.7 0 1 6 1 7.2 0 1