This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.76 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 190,181 (6.0%) Reads that were too short: 92,830 (2.9%) Reads written (passing filters): 97,351 (3.0%) Total basepairs processed: 961,105,341 bp Total written (filtered): 20,118,230 bp (2.1%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 190181 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 836 779.6 0 836 7 24372 779.6 0 24372 8 213 779.6 0 213 9 9994 779.6 0 9994 10 18 779.6 0 18 11 9 779.6 0 9 12 4 779.6 0 4 13 6 779.6 0 6 14 1 779.6 0 1 15 5 779.6 0 5 16 4 779.6 0 4 17 3 779.6 0 3 18 10 779.6 0 10 19 7 779.6 0 7 20 1 779.6 0 1 21 5 779.6 0 5 22 3 779.6 0 3 23 5 779.6 0 5 24 6 779.6 0 6 25 4 779.6 0 4 26 2 779.6 0 2 27 6 779.6 0 6 28 8 779.6 0 8 29 6 779.6 0 6 30 3 779.6 0 3 31 6 779.6 0 6 32 6 779.6 0 6 33 9 779.6 0 9 34 4 779.6 0 4 35 5 779.6 0 5 36 9 779.6 0 9 37 7 779.6 0 7 38 13 779.6 0 13 39 2 779.6 0 2 40 3 779.6 0 3 41 4 779.6 0 4 42 6 779.6 0 6 43 12 779.6 0 12 44 2 779.6 0 2 45 6 779.6 0 6 46 5 779.6 0 5 47 2 779.6 0 2 48 5 779.6 0 5 49 2 779.6 0 2 50 2 779.6 0 2 51 1 779.6 0 1 52 1 779.6 0 1 53 4 779.6 0 4 54 8 779.6 0 8 55 6 779.6 0 6 56 2 779.6 0 2 57 5 779.6 0 5 58 6 779.6 0 6 59 2 779.6 0 2 60 2 779.6 0 2 61 6 779.6 0 6 62 5 779.6 0 5 63 6 779.6 0 6 64 2 779.6 0 2 65 6 779.6 0 6 66 6 779.6 0 6 67 8 779.6 0 8 68 2 779.6 0 2 69 11 779.6 0 11 70 12 779.6 0 12 71 8 779.6 0 8 72 20 779.6 0 20 73 9 779.6 0 9 74 4 779.6 0 4 75 2 779.6 0 2 76 3 779.6 0 3 77 6 779.6 0 6 78 17 779.6 0 17 79 62 779.6 0 62 80 70 779.6 0 70 81 383 779.6 0 383 82 611 779.6 0 611 83 508 779.6 0 508 84 172 779.6 0 172 85 332 779.6 0 332 86 567 779.6 0 567 87 661 779.6 0 661 88 107 779.6 0 107 89 361 779.6 0 361 90 62 779.6 0 62 91 88 779.6 0 88 92 364 779.6 0 364 93 382 779.6 0 382 94 136 779.6 0 136 95 95 779.6 0 95 96 144 779.6 0 144 97 510 779.6 0 510 98 583 779.6 0 583 99 314 779.6 0 314 100 96 779.6 0 96 101 108 779.6 0 108 102 298 779.6 0 298 103 1038 779.6 0 1038 104 347 779.6 0 347 105 162 779.6 0 162 106 281 779.6 0 281 107 114 779.6 0 114 108 379 779.6 0 379 109 1025 779.6 0 1025 110 565 779.6 0 565 111 228 779.6 0 228 112 308 779.6 0 308 113 474 779.6 0 474 114 227 779.6 0 227 115 720 779.6 0 720 116 173 779.6 0 173 117 232 779.6 0 232 118 966 779.6 0 966 119 239 779.6 0 239 120 350 779.6 0 350 121 341 779.6 0 341 122 319 779.6 0 319 123 371 779.6 0 371 124 407 779.6 0 407 125 537 779.6 0 537 126 306 779.6 0 306 127 492 779.6 0 492 128 409 779.6 0 409 129 324 779.6 0 324 130 389 779.6 0 389 131 204 779.6 0 204 132 472 779.6 0 472 133 712 779.6 0 712 134 773 779.6 0 773 135 688 779.6 0 688 136 357 779.6 0 357 137 735 779.6 0 735 138 5048 779.6 0 5048 139 894 779.6 0 894 140 544 779.6 0 544 141 547 779.6 0 547 142 272 779.6 0 272 143 459 779.6 0 459 144 1168 779.6 0 1168 145 414 779.6 0 414 146 672 779.6 0 672 147 417 779.6 0 417 148 471 779.6 0 471 149 617 779.6 0 617 150 501 779.6 0 501 151 1458 779.6 0 1458 152 488 779.6 0 488 153 701 779.6 0 701 154 757 779.6 0 757 155 399 779.6 0 399 156 1033 779.6 0 1033 157 741 779.6 0 741 158 481 779.6 0 481 159 745 779.6 0 745 160 376 779.6 0 376 161 648 779.6 0 648 162 392 779.6 0 392 163 447 779.6 0 447 164 772 779.6 0 772 165 400 779.6 0 400 166 537 779.6 0 537 167 290 779.6 0 290 168 357 779.6 0 357 169 196 779.6 0 196 170 394 779.6 0 394 171 457 779.6 0 457 172 943 779.6 0 943 173 514 779.6 0 514 174 208 779.6 0 208 175 172 779.6 0 172 176 290 779.6 0 290 177 518 779.6 0 518 178 435 779.6 0 435 179 778 779.6 0 778 180 596 779.6 0 596 181 527 779.6 0 527 182 561 779.6 0 561 183 373 779.6 0 373 184 488 779.6 0 488 185 472 779.6 0 472 186 378 779.6 0 378 187 396 779.6 0 396 188 509 779.6 0 509 189 782 779.6 0 782 190 991 779.6 0 991 191 380 779.6 0 380 192 311 779.6 0 311 193 185 779.6 0 185 194 442 779.6 0 442 195 479 779.6 0 479 196 487 779.6 0 487 197 574 779.6 0 574 198 177 779.6 0 177 199 330 779.6 0 330 200 591 779.6 0 591 201 882 779.6 0 882 202 795 779.6 0 795 203 453 779.6 0 453 204 306 779.6 0 306 205 448 779.6 0 448 206 821 779.6 0 821 207 590 779.6 0 590 208 667 779.6 0 667 209 970 779.6 0 970 210 1109 779.6 0 1109 211 9188 779.6 0 9188 212 6591 779.6 0 6591 213 11547 779.6 0 11547 214 3324 779.6 0 3324 215 537 779.6 0 537 216 504 779.6 0 504 217 435 779.6 0 435 218 446 779.6 0 446 219 497 779.6 0 497 220 1413 779.6 0 1413 221 473 779.6 0 473 222 587 779.6 0 587 223 680 779.6 0 680 224 615 779.6 0 615 225 808 779.6 0 808 226 750 779.6 0 750 227 428 779.6 0 428 228 296 779.6 0 296 229 324 779.6 0 324 230 353 779.6 0 353 231 518 779.6 0 518 232 506 779.6 0 506 233 531 779.6 0 531 234 750 779.6 0 750 235 352 779.6 0 352 236 371 779.6 0 371 237 438 779.6 0 438 238 490 779.6 0 490 239 592 779.6 0 592 240 875 779.6 0 875 241 878 779.6 0 878 242 684 779.6 0 684 243 597 779.6 0 597 244 455 779.6 0 455 245 598 779.6 0 598 246 412 779.6 0 412 247 402 779.6 0 402 248 279 779.6 0 279 249 559 779.6 0 559 250 685 779.6 0 685 251 757 779.6 0 757 252 432 779.6 0 432 253 298 779.6 0 298 254 523 779.6 0 523 255 507 779.6 0 507 256 335 779.6 0 335 257 366 779.6 0 366 258 325 779.6 0 325 259 564 779.6 0 564 260 775 779.6 0 775 261 563 779.6 0 563 262 637 779.6 0 637 263 410 779.6 0 410 264 422 779.6 0 422 265 562 779.6 0 562 266 390 779.6 0 390 267 545 779.6 0 545 268 420 779.6 0 420 269 497 779.6 0 497 270 1096 779.6 0 1096 271 983 779.6 0 983 272 1079 779.6 0 1079 273 570 779.6 0 570 274 619 779.6 0 619 275 519 779.6 0 519 276 582 779.6 0 582 277 827 779.6 0 827 278 882 779.6 0 882 279 595 779.6 0 595 280 438 779.6 0 438 281 299 779.6 0 299 282 537 779.6 0 537 283 635 779.6 0 635 284 400 779.6 0 400 285 499 779.6 0 499 286 477 779.6 0 477 287 635 779.6 0 635 288 621 779.6 0 621 289 648 779.6 0 648 290 655 779.6 0 655 291 610 779.6 0 610 292 604 779.6 0 604 293 2645 779.6 0 2645 294 3874 779.6 0 3874 295 1142 779.6 0 1142 296 773 779.6 0 773 297 671 779.6 0 671 298 734 779.6 0 734 299 732 779.6 0 732 300 763 779.6 0 763 301 431 779.6 0 431 Finished in 4.59 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 97,351 Reads with adapters: 25,284 (26.0%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 25,279 (26.0%) Total basepairs processed: 20,118,230 bp Total written (filtered): 6,904,788 bp (34.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25284 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 6 0.0 3 0 0 6 19 240 0.0 3 0 232 5 1 2 20 24745 0.0 4 24275 449 14 4 3 21 56 0.0 4 7 49 22 3 0.0 4 0 1 2 23 23 0.0 4 1 0 0 21 1 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 189 0.0 4 0 0 0 0 189 153 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.07 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 25,279 Reads with adapters: 616 (2.4%) Reads written (passing filters): 25,279 (100.0%) Total basepairs processed: 6,904,788 bp Total written (filtered): 6,815,780 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 616 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.4% C: 50.3% G: 37.5% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 395.0 0 3 4 1 98.7 0 1 11 232 0.0 1 1 231 12 1 0.0 1 0 1 98 2 0.0 1 2 152 11 0.0 1 0 11 153 1 0.0 1 0 1 210 1 0.0 1 1 224 2 0.0 1 2 225 284 0.0 1 266 18 226 13 0.0 1 10 3 234 2 0.0 1 1 1 261 60 0.0 1 58 2 262 3 0.0 1 2 1