This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.45 s (29 us/read; 2.07 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 64,026 (2.0%) Reads that were too short: 10,383 (0.3%) Reads written (passing filters): 53,643 (1.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 9,810,839 bp (1.0%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 64026 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 115 779.6 0 115 7 156 779.6 0 156 8 394 779.6 0 394 9 12139 779.6 0 12139 10 17 779.6 0 17 11 10 779.6 0 10 12 7 779.6 0 7 13 14 779.6 0 14 14 16 779.6 0 16 15 13 779.6 0 13 16 7 779.6 0 7 17 8 779.6 0 8 18 9 779.6 0 9 19 15 779.6 0 15 20 4 779.6 0 4 21 7 779.6 0 7 22 7 779.6 0 7 23 8 779.6 0 8 24 7 779.6 0 7 25 4 779.6 0 4 26 14 779.6 0 14 27 12 779.6 0 12 28 80 779.6 0 80 29 14 779.6 0 14 30 16 779.6 0 16 31 17 779.6 0 17 32 24 779.6 0 24 33 303 779.6 0 303 34 357 779.6 0 357 35 426 779.6 0 426 36 17 779.6 0 17 37 8 779.6 0 8 38 10 779.6 0 10 39 7 779.6 0 7 40 6 779.6 0 6 41 11 779.6 0 11 42 7 779.6 0 7 43 4 779.6 0 4 44 9 779.6 0 9 45 31 779.6 0 31 46 10 779.6 0 10 47 22 779.6 0 22 48 10 779.6 0 10 49 34 779.6 0 34 50 12 779.6 0 12 51 18 779.6 0 18 52 7 779.6 0 7 53 24 779.6 0 24 54 12 779.6 0 12 55 22 779.6 0 22 56 31 779.6 0 31 57 344 779.6 0 344 58 74 779.6 0 74 59 68 779.6 0 68 60 7 779.6 0 7 61 5 779.6 0 5 62 12 779.6 0 12 63 8 779.6 0 8 64 16 779.6 0 16 65 6 779.6 0 6 66 10 779.6 0 10 67 8 779.6 0 8 68 18 779.6 0 18 69 11 779.6 0 11 70 27 779.6 0 27 71 19 779.6 0 19 72 364 779.6 0 364 73 278 779.6 0 278 74 173 779.6 0 173 75 8 779.6 0 8 76 14 779.6 0 14 77 11 779.6 0 11 78 19 779.6 0 19 79 16 779.6 0 16 80 8 779.6 0 8 81 18 779.6 0 18 82 17 779.6 0 17 83 20 779.6 0 20 84 58 779.6 0 58 85 32 779.6 0 32 86 43 779.6 0 43 87 12 779.6 0 12 88 36 779.6 0 36 89 16 779.6 0 16 90 16 779.6 0 16 91 18 779.6 0 18 92 31 779.6 0 31 93 46 779.6 0 46 94 73 779.6 0 73 95 20 779.6 0 20 96 28 779.6 0 28 97 52 779.6 0 52 98 25 779.6 0 25 99 36 779.6 0 36 100 20 779.6 0 20 101 32 779.6 0 32 102 37 779.6 0 37 103 37 779.6 0 37 104 43 779.6 0 43 105 65 779.6 0 65 106 311 779.6 0 311 107 1642 779.6 0 1642 108 1657 779.6 0 1657 109 956 779.6 0 956 110 18 779.6 0 18 111 11 779.6 0 11 112 27 779.6 0 27 113 41 779.6 0 41 114 64 779.6 0 64 115 27 779.6 0 27 116 24 779.6 0 24 117 49 779.6 0 49 118 22 779.6 0 22 119 31 779.6 0 31 120 24 779.6 0 24 121 65 779.6 0 65 122 55 779.6 0 55 123 63 779.6 0 63 124 27 779.6 0 27 125 42 779.6 0 42 126 63 779.6 0 63 127 562 779.6 0 562 128 561 779.6 0 561 129 3428 779.6 0 3428 130 103 779.6 0 103 131 39 779.6 0 39 132 19 779.6 0 19 133 24 779.6 0 24 134 44 779.6 0 44 135 43 779.6 0 43 136 138 779.6 0 138 137 99 779.6 0 99 138 62 779.6 0 62 139 26 779.6 0 26 140 49 779.6 0 49 141 74 779.6 0 74 142 71 779.6 0 71 143 804 779.6 0 804 144 154 779.6 0 154 145 1749 779.6 0 1749 146 37 779.6 0 37 147 205 779.6 0 205 148 82 779.6 0 82 149 151 779.6 0 151 150 14 779.6 0 14 151 23 779.6 0 23 152 40 779.6 0 40 153 210 779.6 0 210 154 155 779.6 0 155 155 323 779.6 0 323 156 154 779.6 0 154 157 36 779.6 0 36 158 176 779.6 0 176 159 60 779.6 0 60 160 29 779.6 0 29 161 50 779.6 0 50 162 47 779.6 0 47 163 82 779.6 0 82 164 25 779.6 0 25 165 29 779.6 0 29 166 47 779.6 0 47 167 43 779.6 0 43 168 43 779.6 0 43 169 119 779.6 0 119 170 16 779.6 0 16 171 52 779.6 0 52 172 21 779.6 0 21 173 39 779.6 0 39 174 69 779.6 0 69 175 70 779.6 0 70 176 44 779.6 0 44 177 21 779.6 0 21 178 26 779.6 0 26 179 53 779.6 0 53 180 67 779.6 0 67 181 97 779.6 0 97 182 154 779.6 0 154 183 218 779.6 0 218 184 305 779.6 0 305 185 308 779.6 0 308 186 3172 779.6 0 3172 187 3003 779.6 0 3003 188 13272 779.6 0 13272 189 68 779.6 0 68 190 14 779.6 0 14 191 21 779.6 0 21 192 30 779.6 0 30 193 20 779.6 0 20 194 15 779.6 0 15 195 50 779.6 0 50 196 7 779.6 0 7 197 12 779.6 0 12 198 14 779.6 0 14 199 11 779.6 0 11 200 15 779.6 0 15 201 24 779.6 0 24 202 16 779.6 0 16 203 23 779.6 0 23 204 59 779.6 0 59 205 48 779.6 0 48 206 32 779.6 0 32 207 21 779.6 0 21 208 48 779.6 0 48 209 45 779.6 0 45 210 26 779.6 0 26 211 34 779.6 0 34 212 44 779.6 0 44 213 35 779.6 0 35 214 38 779.6 0 38 215 51 779.6 0 51 216 83 779.6 0 83 217 293 779.6 0 293 218 729 779.6 0 729 219 3984 779.6 0 3984 220 30 779.6 0 30 221 26 779.6 0 26 222 23 779.6 0 23 223 14 779.6 0 14 224 19 779.6 0 19 225 41 779.6 0 41 226 56 779.6 0 56 227 69 779.6 0 69 228 163 779.6 0 163 229 37 779.6 0 37 230 11 779.6 0 11 231 5 779.6 0 5 232 8 779.6 0 8 233 14 779.6 0 14 234 16 779.6 0 16 235 14 779.6 0 14 236 9 779.6 0 9 237 18 779.6 0 18 238 10 779.6 0 10 239 9 779.6 0 9 240 6 779.6 0 6 241 14 779.6 0 14 242 25 779.6 0 25 243 34 779.6 0 34 244 29 779.6 0 29 245 37 779.6 0 37 246 48 779.6 0 48 247 34 779.6 0 34 248 16 779.6 0 16 249 13 779.6 0 13 250 78 779.6 0 78 251 54 779.6 0 54 252 94 779.6 0 94 253 68 779.6 0 68 254 47 779.6 0 47 255 36 779.6 0 36 256 50 779.6 0 50 257 108 779.6 0 108 258 27 779.6 0 27 259 27 779.6 0 27 260 8 779.6 0 8 261 12 779.6 0 12 262 20 779.6 0 20 263 17 779.6 0 17 264 34 779.6 0 34 265 30 779.6 0 30 266 37 779.6 0 37 267 97 779.6 0 97 268 56 779.6 0 56 269 29 779.6 0 29 270 16 779.6 0 16 271 16 779.6 0 16 272 34 779.6 0 34 273 23 779.6 0 23 274 19 779.6 0 19 275 16 779.6 0 16 276 9 779.6 0 9 277 19 779.6 0 19 278 72 779.6 0 72 279 32 779.6 0 32 280 78 779.6 0 78 281 40 779.6 0 40 282 40 779.6 0 40 283 22 779.6 0 22 284 19 779.6 0 19 285 20 779.6 0 20 286 37 779.6 0 37 287 40 779.6 0 40 288 29 779.6 0 29 289 20 779.6 0 20 290 33 779.6 0 33 291 23 779.6 0 23 292 25 779.6 0 25 293 15 779.6 0 15 294 33 779.6 0 33 295 42 779.6 0 42 296 52 779.6 0 52 297 95 779.6 0 95 298 815 779.6 0 815 299 1016 779.6 0 1016 300 235 779.6 0 235 301 12 779.6 0 12 Finished in 2.04 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 53,643 Reads with adapters: 12,738 (23.7%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 12,708 (23.7%) Total basepairs processed: 9,810,839 bp Total written (filtered): 3,482,253 bp (35.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12738 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 1 2 16 91 0.0 3 0 80 7 4 17 12477 0.0 3 11977 436 52 12 18 24 0.0 3 3 18 1 2 19 11 0.0 3 0 0 8 3 20 17 0.0 3 0 0 0 17 26 1 0.0 3 0 0 0 1 28 1 0.0 3 1 31 3 0.0 3 0 0 0 3 37 5 0.0 3 0 0 0 5 40 1 0.0 3 0 0 0 1 43 5 0.0 3 0 0 0 5 48 17 0.0 3 0 0 0 17 51 7 0.0 3 0 0 0 7 54 1 0.0 3 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 8 0.0 3 0 0 0 8 60 1 0.0 3 0 0 0 1 61 5 0.0 3 0 0 0 5 62 3 0.0 3 0 0 0 3 64 4 0.0 3 0 0 1 3 65 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 84 5 0.0 3 0 0 1 4 91 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 4 0.0 3 0 0 0 4 110 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 134 8 0.0 3 0 0 0 8 135 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 150 8 0.0 3 0 0 0 8 160 2 0.0 3 0 0 0 2 166 4 0.0 3 0 0 0 4 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 12,708 Reads with adapters: 1,378 (10.8%) Reads written (passing filters): 12,708 (100.0%) Total basepairs processed: 3,482,253 bp Total written (filtered): 3,478,025 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1378 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.1% C: 10.1% G: 44.8% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1287 198.6 0 1287 4 89 49.6 0 89 5 1 12.4 0 1 6 1 3.1 0 1