This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.23 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 193,122 (6.0%) Reads that were too short: 154,284 (4.8%) Reads written (passing filters): 38,838 (1.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 8,198,377 bp (0.9%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 193122 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 235 779.6 0 235 7 118 779.6 0 118 8 378 779.6 0 378 9 12593 779.6 0 12593 10 53 779.6 0 53 11 39 779.6 0 39 12 34 779.6 0 34 13 38 779.6 0 38 14 46 779.6 0 46 15 28 779.6 0 28 16 41 779.6 0 41 17 45 779.6 0 45 18 236 779.6 0 236 19 39 779.6 0 39 20 37 779.6 0 37 21 50 779.6 0 50 22 24 779.6 0 24 23 25 779.6 0 25 24 28 779.6 0 28 25 22 779.6 0 22 26 36 779.6 0 36 27 25 779.6 0 25 28 39 779.6 0 39 29 23 779.6 0 23 30 36 779.6 0 36 31 33 779.6 0 33 32 31 779.6 0 31 33 33 779.6 0 33 34 24 779.6 0 24 35 38 779.6 0 38 36 21 779.6 0 21 37 38 779.6 0 38 38 65 779.6 0 65 39 44 779.6 0 44 40 245 779.6 0 245 41 199 779.6 0 199 42 32 779.6 0 32 43 40 779.6 0 40 44 160 779.6 0 160 45 328 779.6 0 328 46 1628 779.6 0 1628 47 99 779.6 0 99 48 110 779.6 0 110 49 25 779.6 0 25 50 68 779.6 0 68 51 23 779.6 0 23 52 45 779.6 0 45 53 33 779.6 0 33 54 53 779.6 0 53 55 76 779.6 0 76 56 33 779.6 0 33 57 77 779.6 0 77 58 42 779.6 0 42 59 17 779.6 0 17 60 22 779.6 0 22 61 33 779.6 0 33 62 25 779.6 0 25 63 26 779.6 0 26 64 39 779.6 0 39 65 30 779.6 0 30 66 24 779.6 0 24 67 43 779.6 0 43 68 31 779.6 0 31 69 22 779.6 0 22 70 24 779.6 0 24 71 15 779.6 0 15 72 38 779.6 0 38 73 63 779.6 0 63 74 41 779.6 0 41 75 55 779.6 0 55 76 23 779.6 0 23 77 48 779.6 0 48 78 47 779.6 0 47 79 51 779.6 0 51 80 31 779.6 0 31 81 51 779.6 0 51 82 67 779.6 0 67 83 33 779.6 0 33 84 53 779.6 0 53 85 53 779.6 0 53 86 58 779.6 0 58 87 25 779.6 0 25 88 60 779.6 0 60 89 84 779.6 0 84 90 52 779.6 0 52 91 27 779.6 0 27 92 34 779.6 0 34 93 43 779.6 0 43 94 73 779.6 0 73 95 80 779.6 0 80 96 96 779.6 0 96 97 62 779.6 0 62 98 56 779.6 0 56 99 329 779.6 0 329 100 85 779.6 0 85 101 40 779.6 0 40 102 38 779.6 0 38 103 30 779.6 0 30 104 40 779.6 0 40 105 65 779.6 0 65 106 107 779.6 0 107 107 44 779.6 0 44 108 45 779.6 0 45 109 44 779.6 0 44 110 37 779.6 0 37 111 35 779.6 0 35 112 40 779.6 0 40 113 31 779.6 0 31 114 53 779.6 0 53 115 66 779.6 0 66 116 86 779.6 0 86 117 98 779.6 0 98 118 117 779.6 0 117 119 121 779.6 0 121 120 153 779.6 0 153 121 94 779.6 0 94 122 51 779.6 0 51 123 63 779.6 0 63 124 68 779.6 0 68 125 83 779.6 0 83 126 103 779.6 0 103 127 82 779.6 0 82 128 53 779.6 0 53 129 79 779.6 0 79 130 86 779.6 0 86 131 66 779.6 0 66 132 48 779.6 0 48 133 68 779.6 0 68 134 102 779.6 0 102 135 156 779.6 0 156 136 223 779.6 0 223 137 111 779.6 0 111 138 109 779.6 0 109 139 90 779.6 0 90 140 113 779.6 0 113 141 73 779.6 0 73 142 42 779.6 0 42 143 56 779.6 0 56 144 111 779.6 0 111 145 595 779.6 0 595 146 1413 779.6 0 1413 147 2417 779.6 0 2417 148 242 779.6 0 242 149 34 779.6 0 34 150 38 779.6 0 38 151 55 779.6 0 55 152 41 779.6 0 41 153 95 779.6 0 95 154 78 779.6 0 78 155 94 779.6 0 94 156 109 779.6 0 109 157 133 779.6 0 133 158 98 779.6 0 98 159 105 779.6 0 105 160 60 779.6 0 60 161 64 779.6 0 64 162 91 779.6 0 91 163 61 779.6 0 61 164 126 779.6 0 126 165 275 779.6 0 275 166 162 779.6 0 162 167 117 779.6 0 117 168 76 779.6 0 76 169 96 779.6 0 96 170 46 779.6 0 46 171 55 779.6 0 55 172 135 779.6 0 135 173 2148 779.6 0 2148 174 429 779.6 0 429 175 439 779.6 0 439 176 273 779.6 0 273 177 49 779.6 0 49 178 66 779.6 0 66 179 113 779.6 0 113 180 149 779.6 0 149 181 552 779.6 0 552 182 613 779.6 0 613 183 402 779.6 0 402 184 317 779.6 0 317 185 144 779.6 0 144 186 483 779.6 0 483 187 215 779.6 0 215 188 558 779.6 0 558 189 54 779.6 0 54 190 101 779.6 0 101 191 162 779.6 0 162 192 202 779.6 0 202 193 321 779.6 0 321 194 124 779.6 0 124 195 52 779.6 0 52 196 52 779.6 0 52 197 105 779.6 0 105 198 97 779.6 0 97 199 75 779.6 0 75 200 66 779.6 0 66 201 66 779.6 0 66 202 106 779.6 0 106 203 236 779.6 0 236 204 130 779.6 0 130 205 141 779.6 0 141 206 154 779.6 0 154 207 309 779.6 0 309 208 165 779.6 0 165 209 101 779.6 0 101 210 94 779.6 0 94 211 156 779.6 0 156 212 278 779.6 0 278 213 167 779.6 0 167 214 166 779.6 0 166 215 208 779.6 0 208 216 162 779.6 0 162 217 133 779.6 0 133 218 198 779.6 0 198 219 214 779.6 0 214 220 171 779.6 0 171 221 231 779.6 0 231 222 189 779.6 0 189 223 286 779.6 0 286 224 372 779.6 0 372 225 4413 779.6 0 4413 226 3920 779.6 0 3920 227 11106 779.6 0 11106 228 266 779.6 0 266 229 625 779.6 0 625 230 581 779.6 0 581 231 734 779.6 0 734 232 588 779.6 0 588 233 854 779.6 0 854 234 425 779.6 0 425 235 170 779.6 0 170 236 84 779.6 0 84 237 140 779.6 0 140 238 102 779.6 0 102 239 89 779.6 0 89 240 106 779.6 0 106 241 105 779.6 0 105 242 133 779.6 0 133 243 193 779.6 0 193 244 167 779.6 0 167 245 126 779.6 0 126 246 512 779.6 0 512 247 618 779.6 0 618 248 645 779.6 0 645 249 112 779.6 0 112 250 2052 779.6 0 2052 251 9529 779.6 0 9529 252 5914 779.6 0 5914 253 276 779.6 0 276 254 276 779.6 0 276 255 256 779.6 0 256 256 256 779.6 0 256 257 139 779.6 0 139 258 106 779.6 0 106 259 91 779.6 0 91 260 96 779.6 0 96 261 93 779.6 0 93 262 128 779.6 0 128 263 147 779.6 0 147 264 233 779.6 0 233 265 984 779.6 0 984 266 364 779.6 0 364 267 231 779.6 0 231 268 175 779.6 0 175 269 167 779.6 0 167 270 170 779.6 0 170 271 798 779.6 0 798 272 1110 779.6 0 1110 273 815 779.6 0 815 274 525 779.6 0 525 275 1886 779.6 0 1886 276 385 779.6 0 385 277 158 779.6 0 158 278 275 779.6 0 275 279 481 779.6 0 481 280 602 779.6 0 602 281 774 779.6 0 774 282 658 779.6 0 658 283 534 779.6 0 534 284 1175 779.6 0 1175 285 2696 779.6 0 2696 286 1007 779.6 0 1007 287 816 779.6 0 816 288 657 779.6 0 657 289 713 779.6 0 713 290 1469 779.6 0 1469 291 1258 779.6 0 1258 292 1440 779.6 0 1440 293 732 779.6 0 732 294 691 779.6 0 691 295 1101 779.6 0 1101 296 1433 779.6 0 1433 297 2726 779.6 0 2726 298 3622 779.6 0 3622 299 6099 779.6 0 6099 300 12007 779.6 0 12007 301 52777 779.6 0 52777 Finished in 1.84 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 38,838 Reads with adapters: 13,117 (33.8%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 13,110 (33.8%) Total basepairs processed: 8,198,377 bp Total written (filtered): 3,566,170 bp (43.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13117 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 25 0.0 3 0 0 0 1 24 18 7 0.0 3 0 0 7 19 170 0.0 3 0 168 1 1 20 12839 0.0 4 12650 181 8 21 45 0.0 4 5 38 2 22 11 0.0 4 2 0 5 1 3 23 4 0.0 4 0 0 0 4 26 1 0.0 4 0 0 0 1 33 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 42 2 0.0 4 2 53 1 0.0 4 0 0 0 0 1 71 4 0.0 4 0 0 0 0 4 73 1 0.0 4 0 0 0 1 81 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.54 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 13,110 Reads with adapters: 187 (1.4%) Reads written (passing filters): 13,110 (100.0%) Total basepairs processed: 3,566,170 bp Total written (filtered): 3,563,558 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 187 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 1.1% G: 94.1% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 204.8 0 1 4 2 51.2 0 2 5 2 12.8 0 2 6 1 3.2 0 1 9 25 0.1 0 1 24 10 68 0.0 1 2 66 11 85 0.0 1 0 85 208 1 0.0 1 0 1 267 1 0.0 1 0 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.