This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.28 s (31 us/read; 1.95 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 221,358 (6.9%) Reads that were too short: 4,523 (0.1%) Reads written (passing filters): 216,835 (6.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 62,538,010 bp (6.5%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 221358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1939 779.6 0 1939 7 4584 779.6 0 4584 8 197027 779.6 0 197027 9 3842 779.6 0 3842 10 45 779.6 0 45 11 185 779.6 0 185 12 23 779.6 0 23 13 18 779.6 0 18 14 11 779.6 0 11 15 15 779.6 0 15 16 14 779.6 0 14 17 11 779.6 0 11 18 24 779.6 0 24 19 17 779.6 0 17 20 6 779.6 0 6 21 12 779.6 0 12 22 11 779.6 0 11 23 9 779.6 0 9 24 10 779.6 0 10 25 15 779.6 0 15 26 13 779.6 0 13 27 14 779.6 0 14 28 16 779.6 0 16 29 8 779.6 0 8 30 17 779.6 0 17 31 13 779.6 0 13 32 13 779.6 0 13 33 13 779.6 0 13 34 10 779.6 0 10 35 11 779.6 0 11 36 15 779.6 0 15 37 8 779.6 0 8 38 15 779.6 0 15 39 12 779.6 0 12 40 18 779.6 0 18 41 15 779.6 0 15 42 37 779.6 0 37 43 10 779.6 0 10 44 9 779.6 0 9 45 12 779.6 0 12 46 16 779.6 0 16 47 23 779.6 0 23 48 13 779.6 0 13 49 278 779.6 0 278 50 18 779.6 0 18 51 3 779.6 0 3 52 8 779.6 0 8 53 13 779.6 0 13 54 12 779.6 0 12 55 14 779.6 0 14 56 10 779.6 0 10 57 10 779.6 0 10 58 34 779.6 0 34 59 27 779.6 0 27 60 42 779.6 0 42 61 29 779.6 0 29 62 21 779.6 0 21 63 6 779.6 0 6 64 28 779.6 0 28 65 4 779.6 0 4 66 110 779.6 0 110 67 20 779.6 0 20 68 42 779.6 0 42 69 8 779.6 0 8 70 9 779.6 0 9 71 6 779.6 0 6 72 32 779.6 0 32 73 8 779.6 0 8 74 7 779.6 0 7 75 6 779.6 0 6 76 8 779.6 0 8 77 8 779.6 0 8 78 8 779.6 0 8 79 13 779.6 0 13 80 7 779.6 0 7 81 6 779.6 0 6 82 39 779.6 0 39 83 26 779.6 0 26 84 18 779.6 0 18 85 38 779.6 0 38 86 18 779.6 0 18 87 18 779.6 0 18 88 4 779.6 0 4 89 14 779.6 0 14 90 14 779.6 0 14 91 64 779.6 0 64 92 29 779.6 0 29 93 31 779.6 0 31 94 28 779.6 0 28 95 20 779.6 0 20 96 10 779.6 0 10 97 75 779.6 0 75 98 122 779.6 0 122 99 13 779.6 0 13 100 12 779.6 0 12 101 13 779.6 0 13 102 10 779.6 0 10 103 15 779.6 0 15 104 126 779.6 0 126 105 10 779.6 0 10 106 13 779.6 0 13 107 47 779.6 0 47 108 37 779.6 0 37 109 18 779.6 0 18 110 21 779.6 0 21 111 140 779.6 0 140 112 71 779.6 0 71 113 187 779.6 0 187 114 49 779.6 0 49 115 84 779.6 0 84 116 119 779.6 0 119 117 1315 779.6 0 1315 118 1026 779.6 0 1026 119 1370 779.6 0 1370 120 40 779.6 0 40 121 14 779.6 0 14 122 17 779.6 0 17 123 14 779.6 0 14 124 12 779.6 0 12 125 21 779.6 0 21 126 11 779.6 0 11 127 16 779.6 0 16 128 44 779.6 0 44 129 13 779.6 0 13 130 12 779.6 0 12 131 19 779.6 0 19 132 12 779.6 0 12 133 28 779.6 0 28 134 56 779.6 0 56 135 18 779.6 0 18 136 12 779.6 0 12 137 14 779.6 0 14 138 18 779.6 0 18 139 19 779.6 0 19 140 17 779.6 0 17 141 13 779.6 0 13 142 14 779.6 0 14 143 23 779.6 0 23 144 26 779.6 0 26 145 28 779.6 0 28 146 35 779.6 0 35 147 32 779.6 0 32 148 26 779.6 0 26 149 30 779.6 0 30 150 33 779.6 0 33 151 171 779.6 0 171 152 228 779.6 0 228 153 305 779.6 0 305 154 47 779.6 0 47 155 17 779.6 0 17 156 92 779.6 0 92 157 21 779.6 0 21 158 17 779.6 0 17 159 15 779.6 0 15 160 17 779.6 0 17 161 18 779.6 0 18 162 36 779.6 0 36 163 37 779.6 0 37 164 28 779.6 0 28 165 21 779.6 0 21 166 23 779.6 0 23 167 14 779.6 0 14 168 17 779.6 0 17 169 13 779.6 0 13 170 25 779.6 0 25 171 39 779.6 0 39 172 10 779.6 0 10 173 14 779.6 0 14 174 10 779.6 0 10 175 10 779.6 0 10 176 11 779.6 0 11 177 25 779.6 0 25 178 7 779.6 0 7 179 19 779.6 0 19 180 115 779.6 0 115 181 50 779.6 0 50 182 68 779.6 0 68 183 23 779.6 0 23 184 44 779.6 0 44 185 17 779.6 0 17 186 14 779.6 0 14 187 15 779.6 0 15 188 13 779.6 0 13 189 17 779.6 0 17 190 22 779.6 0 22 191 11 779.6 0 11 192 24 779.6 0 24 193 13 779.6 0 13 194 4 779.6 0 4 195 35 779.6 0 35 196 21 779.6 0 21 197 9 779.6 0 9 198 8 779.6 0 8 199 9 779.6 0 9 200 18 779.6 0 18 201 23 779.6 0 23 202 9 779.6 0 9 203 7 779.6 0 7 204 11 779.6 0 11 205 9 779.6 0 9 206 13 779.6 0 13 207 16 779.6 0 16 208 31 779.6 0 31 209 10 779.6 0 10 210 31 779.6 0 31 211 50 779.6 0 50 212 21 779.6 0 21 213 23 779.6 0 23 214 29 779.6 0 29 215 21 779.6 0 21 216 36 779.6 0 36 217 51 779.6 0 51 218 62 779.6 0 62 219 168 779.6 0 168 220 107 779.6 0 107 221 53 779.6 0 53 222 22 779.6 0 22 223 12 779.6 0 12 224 15 779.6 0 15 225 38 779.6 0 38 226 56 779.6 0 56 227 33 779.6 0 33 228 35 779.6 0 35 229 40 779.6 0 40 230 34 779.6 0 34 231 76 779.6 0 76 232 229 779.6 0 229 233 307 779.6 0 307 234 43 779.6 0 43 235 26 779.6 0 26 236 60 779.6 0 60 237 58 779.6 0 58 238 50 779.6 0 50 239 40 779.6 0 40 240 25 779.6 0 25 241 20 779.6 0 20 242 19 779.6 0 19 243 21 779.6 0 21 244 20 779.6 0 20 245 30 779.6 0 30 246 61 779.6 0 61 247 56 779.6 0 56 248 67 779.6 0 67 249 61 779.6 0 61 250 108 779.6 0 108 251 46 779.6 0 46 252 45 779.6 0 45 253 66 779.6 0 66 254 79 779.6 0 79 255 145 779.6 0 145 256 268 779.6 0 268 257 31 779.6 0 31 258 25 779.6 0 25 259 24 779.6 0 24 260 14 779.6 0 14 261 19 779.6 0 19 262 15 779.6 0 15 263 18 779.6 0 18 264 25 779.6 0 25 265 25 779.6 0 25 266 19 779.6 0 19 267 15 779.6 0 15 268 37 779.6 0 37 269 42 779.6 0 42 270 37 779.6 0 37 271 19 779.6 0 19 272 19 779.6 0 19 273 13 779.6 0 13 274 21 779.6 0 21 275 13 779.6 0 13 276 24 779.6 0 24 277 26 779.6 0 26 278 22 779.6 0 22 279 21 779.6 0 21 280 47 779.6 0 47 281 52 779.6 0 52 282 185 779.6 0 185 283 113 779.6 0 113 284 62 779.6 0 62 285 17 779.6 0 17 286 21 779.6 0 21 287 16 779.6 0 16 288 30 779.6 0 30 289 26 779.6 0 26 290 60 779.6 0 60 291 46 779.6 0 46 292 16 779.6 0 16 293 28 779.6 0 28 294 29 779.6 0 29 295 47 779.6 0 47 296 29 779.6 0 29 297 27 779.6 0 27 298 17 779.6 0 17 299 14 779.6 0 14 300 41 779.6 0 41 301 7 779.6 0 7 Finished in 9.71 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 216,835 Reads with adapters: 202,242 (93.3%) Reads that were too short: 63 (0.0%) Reads written (passing filters): 202,179 (93.2%) Total basepairs processed: 62,538,010 bp Total written (filtered): 55,793,118 bp (89.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 202242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 68 0.0 3 0 0 56 12 16 1949 0.0 3 0 1669 221 59 17 197481 0.0 3 192029 4845 487 120 18 717 0.0 3 55 481 69 112 19 128 0.0 3 1 1 79 47 20 1736 0.0 3 3 0 1 1732 21 3 0.0 3 1 1 0 1 22 2 0.0 3 2 24 2 0.0 3 2 26 1 0.0 3 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 1 33 1 0.0 3 0 1 40 9 0.0 3 0 0 0 9 43 6 0.0 3 0 0 0 6 51 1 0.0 3 0 0 0 1 54 16 0.0 3 0 0 0 16 56 4 0.0 3 0 0 0 4 57 7 0.0 3 0 0 0 7 62 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 1 1 141 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 166 17 0.0 3 0 0 0 17 167 2 0.0 3 0 0 0 2 169 31 0.0 3 0 0 2 29 171 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 10 0.0 3 0 0 0 10 186 3 0.0 3 0 0 0 3 187 4 0.0 3 0 0 0 4 211 1 0.0 3 0 0 1 217 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.29 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 202,179 Reads with adapters: 1,194 (0.6%) Reads written (passing filters): 202,179 (100.0%) Total basepairs processed: 55,793,118 bp Total written (filtered): 55,789,389 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1194 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.3% C: 7.8% G: 52.3% T: 14.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1051 3159.0 0 1051 4 139 789.8 0 139 5 4 197.4 0 4