This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 97.28 s (30 us/read; 1.97 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 241,653 (7.6%) Reads that were too short: 27,168 (0.9%) Reads written (passing filters): 214,485 (6.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 61,754,259 bp (6.4%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 241653 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1765 779.6 0 1765 7 6127 779.6 0 6127 8 196123 779.6 0 196123 9 260 779.6 0 260 10 21 779.6 0 21 11 14 779.6 0 14 12 11 779.6 0 11 13 12 779.6 0 12 14 14 779.6 0 14 15 9 779.6 0 9 16 5 779.6 0 5 17 12 779.6 0 12 18 14 779.6 0 14 19 14 779.6 0 14 20 9 779.6 0 9 21 18 779.6 0 18 22 22 779.6 0 22 23 66 779.6 0 66 24 17 779.6 0 17 25 7 779.6 0 7 26 12 779.6 0 12 27 14 779.6 0 14 28 18 779.6 0 18 29 6 779.6 0 6 30 8 779.6 0 8 31 51 779.6 0 51 32 18 779.6 0 18 33 17 779.6 0 17 34 13 779.6 0 13 35 15 779.6 0 15 36 9 779.6 0 9 37 7 779.6 0 7 38 151 779.6 0 151 39 93 779.6 0 93 40 45 779.6 0 45 41 70 779.6 0 70 42 34 779.6 0 34 43 26 779.6 0 26 44 14 779.6 0 14 45 16 779.6 0 16 46 22 779.6 0 22 47 11 779.6 0 11 48 7 779.6 0 7 49 12 779.6 0 12 50 19 779.6 0 19 51 28 779.6 0 28 52 7 779.6 0 7 53 11 779.6 0 11 54 12 779.6 0 12 55 14 779.6 0 14 56 13 779.6 0 13 57 14 779.6 0 14 58 19 779.6 0 19 59 16 779.6 0 16 60 21 779.6 0 21 61 19 779.6 0 19 62 123 779.6 0 123 63 29 779.6 0 29 64 41 779.6 0 41 65 1388 779.6 0 1388 66 325 779.6 0 325 67 13 779.6 0 13 68 17 779.6 0 17 69 21 779.6 0 21 70 15 779.6 0 15 71 22 779.6 0 22 72 33 779.6 0 33 73 19 779.6 0 19 74 18 779.6 0 18 75 12 779.6 0 12 76 19 779.6 0 19 77 23 779.6 0 23 78 13 779.6 0 13 79 17 779.6 0 17 80 18 779.6 0 18 81 24 779.6 0 24 82 44 779.6 0 44 83 18 779.6 0 18 84 11 779.6 0 11 85 56 779.6 0 56 86 54 779.6 0 54 87 21 779.6 0 21 88 12 779.6 0 12 89 12 779.6 0 12 90 13 779.6 0 13 91 37 779.6 0 37 92 24 779.6 0 24 93 24 779.6 0 24 94 23 779.6 0 23 95 39 779.6 0 39 96 49 779.6 0 49 97 31 779.6 0 31 98 144 779.6 0 144 99 131 779.6 0 131 100 168 779.6 0 168 101 55 779.6 0 55 102 79 779.6 0 79 103 65 779.6 0 65 104 56 779.6 0 56 105 43 779.6 0 43 106 45 779.6 0 45 107 44 779.6 0 44 108 22 779.6 0 22 109 26 779.6 0 26 110 21 779.6 0 21 111 135 779.6 0 135 112 51 779.6 0 51 113 208 779.6 0 208 114 26 779.6 0 26 115 151 779.6 0 151 116 35 779.6 0 35 117 25 779.6 0 25 118 33 779.6 0 33 119 31 779.6 0 31 120 50 779.6 0 50 121 47 779.6 0 47 122 40 779.6 0 40 123 70 779.6 0 70 124 73 779.6 0 73 125 53 779.6 0 53 126 47 779.6 0 47 127 32 779.6 0 32 128 27 779.6 0 27 129 21 779.6 0 21 130 20 779.6 0 20 131 29 779.6 0 29 132 48 779.6 0 48 133 64 779.6 0 64 134 40 779.6 0 40 135 49 779.6 0 49 136 42 779.6 0 42 137 60 779.6 0 60 138 72 779.6 0 72 139 86 779.6 0 86 140 75 779.6 0 75 141 55 779.6 0 55 142 42 779.6 0 42 143 127 779.6 0 127 144 141 779.6 0 141 145 99 779.6 0 99 146 68 779.6 0 68 147 59 779.6 0 59 148 92 779.6 0 92 149 47 779.6 0 47 150 32 779.6 0 32 151 26 779.6 0 26 152 21 779.6 0 21 153 19 779.6 0 19 154 26 779.6 0 26 155 35 779.6 0 35 156 31 779.6 0 31 157 17 779.6 0 17 158 73 779.6 0 73 159 31 779.6 0 31 160 108 779.6 0 108 161 55 779.6 0 55 162 29 779.6 0 29 163 38 779.6 0 38 164 39 779.6 0 39 165 21 779.6 0 21 166 38 779.6 0 38 167 33 779.6 0 33 168 55 779.6 0 55 169 108 779.6 0 108 170 235 779.6 0 235 171 79 779.6 0 79 172 57 779.6 0 57 173 153 779.6 0 153 174 30 779.6 0 30 175 29 779.6 0 29 176 21 779.6 0 21 177 49 779.6 0 49 178 46 779.6 0 46 179 42 779.6 0 42 180 33 779.6 0 33 181 30 779.6 0 30 182 33 779.6 0 33 183 81 779.6 0 81 184 124 779.6 0 124 185 136 779.6 0 136 186 67 779.6 0 67 187 186 779.6 0 186 188 153 779.6 0 153 189 81 779.6 0 81 190 23 779.6 0 23 191 22 779.6 0 22 192 24 779.6 0 24 193 38 779.6 0 38 194 56 779.6 0 56 195 36 779.6 0 36 196 32 779.6 0 32 197 32 779.6 0 32 198 57 779.6 0 57 199 137 779.6 0 137 200 56 779.6 0 56 201 23 779.6 0 23 202 14 779.6 0 14 203 16 779.6 0 16 204 50 779.6 0 50 205 71 779.6 0 71 206 82 779.6 0 82 207 36 779.6 0 36 208 42 779.6 0 42 209 28 779.6 0 28 210 55 779.6 0 55 211 97 779.6 0 97 212 68 779.6 0 68 213 75 779.6 0 75 214 251 779.6 0 251 215 747 779.6 0 747 216 703 779.6 0 703 217 66 779.6 0 66 218 73 779.6 0 73 219 109 779.6 0 109 220 73 779.6 0 73 221 68 779.6 0 68 222 102 779.6 0 102 223 1396 779.6 0 1396 224 298 779.6 0 298 225 2052 779.6 0 2052 226 59 779.6 0 59 227 51 779.6 0 51 228 64 779.6 0 64 229 90 779.6 0 90 230 247 779.6 0 247 231 52 779.6 0 52 232 25 779.6 0 25 233 19 779.6 0 19 234 26 779.6 0 26 235 42 779.6 0 42 236 29 779.6 0 29 237 50 779.6 0 50 238 163 779.6 0 163 239 183 779.6 0 183 240 112 779.6 0 112 241 183 779.6 0 183 242 24 779.6 0 24 243 41 779.6 0 41 244 16 779.6 0 16 245 63 779.6 0 63 246 106 779.6 0 106 247 103 779.6 0 103 248 67 779.6 0 67 249 28 779.6 0 28 250 39 779.6 0 39 251 17 779.6 0 17 252 16 779.6 0 16 253 55 779.6 0 55 254 89 779.6 0 89 255 70 779.6 0 70 256 215 779.6 0 215 257 108 779.6 0 108 258 97 779.6 0 97 259 128 779.6 0 128 260 72 779.6 0 72 261 93 779.6 0 93 262 41 779.6 0 41 263 340 779.6 0 340 264 885 779.6 0 885 265 1455 779.6 0 1455 266 112 779.6 0 112 267 230 779.6 0 230 268 285 779.6 0 285 269 272 779.6 0 272 270 207 779.6 0 207 271 129 779.6 0 129 272 932 779.6 0 932 273 2408 779.6 0 2408 274 7097 779.6 0 7097 275 104 779.6 0 104 276 56 779.6 0 56 277 61 779.6 0 61 278 94 779.6 0 94 279 200 779.6 0 200 280 168 779.6 0 168 281 27 779.6 0 27 282 26 779.6 0 26 283 47 779.6 0 47 284 77 779.6 0 77 285 145 779.6 0 145 286 302 779.6 0 302 287 108 779.6 0 108 288 69 779.6 0 69 289 25 779.6 0 25 290 12 779.6 0 12 291 19 779.6 0 19 292 53 779.6 0 53 293 100 779.6 0 100 294 84 779.6 0 84 295 75 779.6 0 75 296 75 779.6 0 75 297 42 779.6 0 42 298 33 779.6 0 33 299 64 779.6 0 64 300 174 779.6 0 174 301 1121 779.6 0 1121 Finished in 9.58 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 214,485 Reads with adapters: 203,947 (95.1%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 203,943 (95.1%) Total basepairs processed: 61,754,259 bp Total written (filtered): 55,685,333 bp (90.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 203947 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 91 0.0 3 0 0 86 4 1 19 2148 0.0 3 0 2097 34 12 5 20 200952 0.0 4 197724 3095 111 14 8 21 587 0.0 4 124 460 2 1 22 38 0.0 4 0 0 34 2 2 23 102 0.0 4 4 0 1 89 8 24 8 0.0 4 0 0 1 0 7 26 1 0.0 4 0 0 1 27 4 0.0 4 2 0 1 0 1 28 3 0.0 4 2 1 31 2 0.0 4 1 1 32 1 0.0 4 1 36 4 0.0 4 0 0 0 0 4 40 1 0.0 4 1 132 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.08 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 203,943 Reads with adapters: 7,960 (3.9%) Reads written (passing filters): 203,943 (100.0%) Total basepairs processed: 55,685,333 bp Total written (filtered): 55,561,872 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7960 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 0.5% G: 96.4% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 3186.6 0 5 4 16 796.7 0 16 5 2 199.2 0 2 9 1 0.8 0 0 1 10 40 0.2 1 5 35 11 7744 0.0 1 9 7735 12 2 0.0 1 0 2 209 25 0.0 1 7 18 211 3 0.0 1 0 3 224 2 0.0 1 1 1 261 1 0.0 1 1 262 110 0.0 1 104 6 263 8 0.0 1 8 264 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.