This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 97.55 s (31 us/read; 1.96 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 196,484 (6.2%) Reads that were too short: 20,432 (0.6%) Reads written (passing filters): 176,052 (5.5%) Total basepairs processed: 961,105,341 bp Total written (filtered): 37,165,891 bp (3.9%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 196484 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1848 779.6 0 1848 7 60633 779.6 0 60633 8 111 779.6 0 111 9 53 779.6 0 53 10 25 779.6 0 25 11 31 779.6 0 31 12 17 779.6 0 17 13 34 779.6 0 34 14 18 779.6 0 18 15 23 779.6 0 23 16 24 779.6 0 24 17 23 779.6 0 23 18 33 779.6 0 33 19 31 779.6 0 31 20 18 779.6 0 18 21 45 779.6 0 45 22 36 779.6 0 36 23 37 779.6 0 37 24 43 779.6 0 43 25 79 779.6 0 79 26 270 779.6 0 270 27 35 779.6 0 35 28 24 779.6 0 24 29 38 779.6 0 38 30 28 779.6 0 28 31 23 779.6 0 23 32 26 779.6 0 26 33 39 779.6 0 39 34 20 779.6 0 20 35 28 779.6 0 28 36 23 779.6 0 23 37 25 779.6 0 25 38 23 779.6 0 23 39 36 779.6 0 36 40 28 779.6 0 28 41 31 779.6 0 31 42 23 779.6 0 23 43 21 779.6 0 21 44 22 779.6 0 22 45 30 779.6 0 30 46 22 779.6 0 22 47 39 779.6 0 39 48 34 779.6 0 34 49 32 779.6 0 32 50 25 779.6 0 25 51 41 779.6 0 41 52 23 779.6 0 23 53 27 779.6 0 27 54 45 779.6 0 45 55 96 779.6 0 96 56 32 779.6 0 32 57 32 779.6 0 32 58 29 779.6 0 29 59 51 779.6 0 51 60 44 779.6 0 44 61 103 779.6 0 103 62 184 779.6 0 184 63 347 779.6 0 347 64 31 779.6 0 31 65 20 779.6 0 20 66 32 779.6 0 32 67 28 779.6 0 28 68 36 779.6 0 36 69 44 779.6 0 44 70 71 779.6 0 71 71 64 779.6 0 64 72 27 779.6 0 27 73 28 779.6 0 28 74 40 779.6 0 40 75 38 779.6 0 38 76 22 779.6 0 22 77 37 779.6 0 37 78 90 779.6 0 90 79 78 779.6 0 78 80 169 779.6 0 169 81 82 779.6 0 82 82 237 779.6 0 237 83 241 779.6 0 241 84 205 779.6 0 205 85 174 779.6 0 174 86 26 779.6 0 26 87 70 779.6 0 70 88 52 779.6 0 52 89 41 779.6 0 41 90 61 779.6 0 61 91 39 779.6 0 39 92 27 779.6 0 27 93 90 779.6 0 90 94 136 779.6 0 136 95 69 779.6 0 69 96 71 779.6 0 71 97 104 779.6 0 104 98 75 779.6 0 75 99 214 779.6 0 214 100 175 779.6 0 175 101 175 779.6 0 175 102 140 779.6 0 140 103 111 779.6 0 111 104 78 779.6 0 78 105 132 779.6 0 132 106 168 779.6 0 168 107 252 779.6 0 252 108 193 779.6 0 193 109 219 779.6 0 219 110 264 779.6 0 264 111 520 779.6 0 520 112 5709 779.6 0 5709 113 4006 779.6 0 4006 114 6819 779.6 0 6819 115 355 779.6 0 355 116 255 779.6 0 255 117 201 779.6 0 201 118 512 779.6 0 512 119 659 779.6 0 659 120 2036 779.6 0 2036 121 1691 779.6 0 1691 122 7013 779.6 0 7013 123 402 779.6 0 402 124 529 779.6 0 529 125 4246 779.6 0 4246 126 26214 779.6 0 26214 127 12106 779.6 0 12106 128 134 779.6 0 134 129 214 779.6 0 214 130 91 779.6 0 91 131 148 779.6 0 148 132 116 779.6 0 116 133 94 779.6 0 94 134 119 779.6 0 119 135 97 779.6 0 97 136 124 779.6 0 124 137 134 779.6 0 134 138 138 779.6 0 138 139 127 779.6 0 127 140 124 779.6 0 124 141 106 779.6 0 106 142 104 779.6 0 104 143 104 779.6 0 104 144 135 779.6 0 135 145 145 779.6 0 145 146 166 779.6 0 166 147 198 779.6 0 198 148 305 779.6 0 305 149 205 779.6 0 205 150 130 779.6 0 130 151 74 779.6 0 74 152 101 779.6 0 101 153 124 779.6 0 124 154 174 779.6 0 174 155 106 779.6 0 106 156 99 779.6 0 99 157 173 779.6 0 173 158 168 779.6 0 168 159 140 779.6 0 140 160 130 779.6 0 130 161 198 779.6 0 198 162 146 779.6 0 146 163 145 779.6 0 145 164 192 779.6 0 192 165 370 779.6 0 370 166 371 779.6 0 371 167 461 779.6 0 461 168 440 779.6 0 440 169 212 779.6 0 212 170 409 779.6 0 409 171 824 779.6 0 824 172 838 779.6 0 838 173 1040 779.6 0 1040 174 1357 779.6 0 1357 175 129 779.6 0 129 176 139 779.6 0 139 177 135 779.6 0 135 178 180 779.6 0 180 179 271 779.6 0 271 180 278 779.6 0 278 181 209 779.6 0 209 182 214 779.6 0 214 183 824 779.6 0 824 184 1119 779.6 0 1119 185 2722 779.6 0 2722 186 155 779.6 0 155 187 135 779.6 0 135 188 147 779.6 0 147 189 143 779.6 0 143 190 255 779.6 0 255 191 3135 779.6 0 3135 192 7352 779.6 0 7352 193 1307 779.6 0 1307 194 362 779.6 0 362 195 248 779.6 0 248 196 189 779.6 0 189 197 181 779.6 0 181 198 256 779.6 0 256 199 555 779.6 0 555 200 521 779.6 0 521 201 131 779.6 0 131 202 112 779.6 0 112 203 147 779.6 0 147 204 182 779.6 0 182 205 101 779.6 0 101 206 110 779.6 0 110 207 102 779.6 0 102 208 80 779.6 0 80 209 177 779.6 0 177 210 134 779.6 0 134 211 163 779.6 0 163 212 153 779.6 0 153 213 142 779.6 0 142 214 137 779.6 0 137 215 214 779.6 0 214 216 170 779.6 0 170 217 219 779.6 0 219 218 176 779.6 0 176 219 147 779.6 0 147 220 218 779.6 0 218 221 71 779.6 0 71 222 75 779.6 0 75 223 205 779.6 0 205 224 174 779.6 0 174 225 169 779.6 0 169 226 158 779.6 0 158 227 183 779.6 0 183 228 288 779.6 0 288 229 387 779.6 0 387 230 274 779.6 0 274 231 156 779.6 0 156 232 158 779.6 0 158 233 107 779.6 0 107 234 133 779.6 0 133 235 257 779.6 0 257 236 125 779.6 0 125 237 130 779.6 0 130 238 99 779.6 0 99 239 90 779.6 0 90 240 82 779.6 0 82 241 96 779.6 0 96 242 88 779.6 0 88 243 172 779.6 0 172 244 190 779.6 0 190 245 277 779.6 0 277 246 114 779.6 0 114 247 169 779.6 0 169 248 59 779.6 0 59 249 64 779.6 0 64 250 91 779.6 0 91 251 299 779.6 0 299 252 480 779.6 0 480 253 309 779.6 0 309 254 168 779.6 0 168 255 186 779.6 0 186 256 182 779.6 0 182 257 272 779.6 0 272 258 96 779.6 0 96 259 76 779.6 0 76 260 71 779.6 0 71 261 105 779.6 0 105 262 166 779.6 0 166 263 211 779.6 0 211 264 164 779.6 0 164 265 194 779.6 0 194 266 141 779.6 0 141 267 878 779.6 0 878 268 370 779.6 0 370 269 2354 779.6 0 2354 270 129 779.6 0 129 271 130 779.6 0 130 272 123 779.6 0 123 273 142 779.6 0 142 274 148 779.6 0 148 275 111 779.6 0 111 276 113 779.6 0 113 277 153 779.6 0 153 278 208 779.6 0 208 279 150 779.6 0 150 280 180 779.6 0 180 281 115 779.6 0 115 282 120 779.6 0 120 283 414 779.6 0 414 284 313 779.6 0 313 285 159 779.6 0 159 286 125 779.6 0 125 287 121 779.6 0 121 288 98 779.6 0 98 289 117 779.6 0 117 290 170 779.6 0 170 291 248 779.6 0 248 292 268 779.6 0 268 293 428 779.6 0 428 294 196 779.6 0 196 295 115 779.6 0 115 296 238 779.6 0 238 297 764 779.6 0 764 298 198 779.6 0 198 299 149 779.6 0 149 300 195 779.6 0 195 301 47 779.6 0 47 Finished in 7.87 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 176,052 Reads with adapters: 62,406 (35.4%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 62,394 (35.4%) Total basepairs processed: 37,165,891 bp Total written (filtered): 17,096,613 bp (46.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 62406 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 0 8 18 26 0.0 3 0 0 24 2 19 576 0.0 3 0 559 11 4 2 20 61558 0.0 4 60614 910 26 6 2 21 142 0.0 4 31 109 2 22 15 0.0 4 1 0 13 0 1 23 55 0.0 4 5 0 0 49 1 24 3 0.0 4 0 0 0 1 2 28 1 0.0 4 1 31 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 51 3 0.0 4 0 0 0 0 3 55 1 0.0 4 0 1 85 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 110 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.45 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 62,394 Reads with adapters: 442 (0.7%) Reads written (passing filters): 62,394 (100.0%) Total basepairs processed: 17,096,613 bp Total written (filtered): 17,088,996 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 442 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 3.2% G: 93.0% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 974.9 0 6 4 66 243.7 0 66 6 1 15.2 0 1 9 1 0.2 0 0 1 11 344 0.0 1 3 341 12 6 0.0 1 1 5 15 1 0.0 1 0 1 48 1 0.0 1 1 150 1 0.0 1 1 171 2 0.0 1 2 210 4 0.0 1 0 4 225 8 0.0 1 7 1 269 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.