This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 99.05 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 691,671 (21.7%) Reads that were too short: 11,972 (0.4%) Reads written (passing filters): 679,699 (21.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 155,346,330 bp (16.2%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 691671 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3101 779.6 0 3101 7 10699 779.6 0 10699 8 2696 779.6 0 2696 9 142335 779.6 0 142335 10 265 779.6 0 265 11 32 779.6 0 32 12 47 779.6 0 47 13 15 779.6 0 15 14 21 779.6 0 21 15 34 779.6 0 34 16 22 779.6 0 22 17 40 779.6 0 40 18 32 779.6 0 32 19 28 779.6 0 28 20 37 779.6 0 37 21 37 779.6 0 37 22 26 779.6 0 26 23 11 779.6 0 11 24 27 779.6 0 27 25 27 779.6 0 27 26 12 779.6 0 12 27 20 779.6 0 20 28 14 779.6 0 14 29 19 779.6 0 19 30 29 779.6 0 29 31 28 779.6 0 28 32 22 779.6 0 22 33 17 779.6 0 17 34 15 779.6 0 15 35 30 779.6 0 30 36 34 779.6 0 34 37 28 779.6 0 28 38 35 779.6 0 35 39 22 779.6 0 22 40 19 779.6 0 19 41 29 779.6 0 29 42 25 779.6 0 25 43 26 779.6 0 26 44 39 779.6 0 39 45 26 779.6 0 26 46 28 779.6 0 28 47 28 779.6 0 28 48 23 779.6 0 23 49 16 779.6 0 16 50 21 779.6 0 21 51 100 779.6 0 100 52 45 779.6 0 45 53 51 779.6 0 51 54 81 779.6 0 81 55 58 779.6 0 58 56 53 779.6 0 53 57 111 779.6 0 111 58 42 779.6 0 42 59 106 779.6 0 106 60 117 779.6 0 117 61 102 779.6 0 102 62 355 779.6 0 355 63 107 779.6 0 107 64 82 779.6 0 82 65 60 779.6 0 60 66 206 779.6 0 206 67 144 779.6 0 144 68 321 779.6 0 321 69 468 779.6 0 468 70 554 779.6 0 554 71 535 779.6 0 535 72 1284 779.6 0 1284 73 3386 779.6 0 3386 74 926 779.6 0 926 75 1109 779.6 0 1109 76 2055 779.6 0 2055 77 1107 779.6 0 1107 78 1023 779.6 0 1023 79 2335 779.6 0 2335 80 3094 779.6 0 3094 81 3072 779.6 0 3072 82 3018 779.6 0 3018 83 5549 779.6 0 5549 84 5677 779.6 0 5677 85 6225 779.6 0 6225 86 68214 779.6 0 68214 87 283444 779.6 0 283444 88 85875 779.6 0 85875 89 383 779.6 0 383 90 35 779.6 0 35 91 18 779.6 0 18 92 25 779.6 0 25 93 44 779.6 0 44 94 45 779.6 0 45 95 22 779.6 0 22 96 16 779.6 0 16 97 19 779.6 0 19 98 14 779.6 0 14 99 42 779.6 0 42 100 63 779.6 0 63 101 43 779.6 0 43 102 23 779.6 0 23 103 27 779.6 0 27 104 24 779.6 0 24 105 116 779.6 0 116 106 10 779.6 0 10 107 13 779.6 0 13 108 6 779.6 0 6 109 15 779.6 0 15 110 34 779.6 0 34 111 23 779.6 0 23 112 26 779.6 0 26 113 16 779.6 0 16 114 18 779.6 0 18 115 29 779.6 0 29 116 37 779.6 0 37 117 41 779.6 0 41 118 48 779.6 0 48 119 55 779.6 0 55 120 49 779.6 0 49 121 45 779.6 0 45 122 48 779.6 0 48 123 275 779.6 0 275 124 569 779.6 0 569 125 963 779.6 0 963 126 635 779.6 0 635 127 361 779.6 0 361 128 73 779.6 0 73 129 70 779.6 0 70 130 92 779.6 0 92 131 200 779.6 0 200 132 47 779.6 0 47 133 39 779.6 0 39 134 71 779.6 0 71 135 49 779.6 0 49 136 73 779.6 0 73 137 92 779.6 0 92 138 65 779.6 0 65 139 94 779.6 0 94 140 86 779.6 0 86 141 101 779.6 0 101 142 145 779.6 0 145 143 236 779.6 0 236 144 2868 779.6 0 2868 145 3015 779.6 0 3015 146 4345 779.6 0 4345 147 83 779.6 0 83 148 17 779.6 0 17 149 29 779.6 0 29 150 16 779.6 0 16 151 21 779.6 0 21 152 34 779.6 0 34 153 22 779.6 0 22 154 20 779.6 0 20 155 29 779.6 0 29 156 54 779.6 0 54 157 54 779.6 0 54 158 31 779.6 0 31 159 37 779.6 0 37 160 28 779.6 0 28 161 44 779.6 0 44 162 39 779.6 0 39 163 48 779.6 0 48 164 69 779.6 0 69 165 92 779.6 0 92 166 1261 779.6 0 1261 167 506 779.6 0 506 168 1360 779.6 0 1360 169 47 779.6 0 47 170 48 779.6 0 48 171 85 779.6 0 85 172 213 779.6 0 213 173 62 779.6 0 62 174 74 779.6 0 74 175 67 779.6 0 67 176 32 779.6 0 32 177 57 779.6 0 57 178 87 779.6 0 87 179 120 779.6 0 120 180 128 779.6 0 128 181 108 779.6 0 108 182 125 779.6 0 125 183 187 779.6 0 187 184 335 779.6 0 335 185 4914 779.6 0 4914 186 3482 779.6 0 3482 187 7627 779.6 0 7627 188 48 779.6 0 48 189 22 779.6 0 22 190 39 779.6 0 39 191 92 779.6 0 92 192 64 779.6 0 64 193 81 779.6 0 81 194 29 779.6 0 29 195 48 779.6 0 48 196 40 779.6 0 40 197 60 779.6 0 60 198 97 779.6 0 97 199 43 779.6 0 43 200 28 779.6 0 28 201 52 779.6 0 52 202 48 779.6 0 48 203 94 779.6 0 94 204 110 779.6 0 110 205 125 779.6 0 125 206 1234 779.6 0 1234 207 995 779.6 0 995 208 2258 779.6 0 2258 209 60 779.6 0 60 210 197 779.6 0 197 211 631 779.6 0 631 212 188 779.6 0 188 213 348 779.6 0 348 214 22 779.6 0 22 215 22 779.6 0 22 216 29 779.6 0 29 217 32 779.6 0 32 218 20 779.6 0 20 219 14 779.6 0 14 220 9 779.6 0 9 221 16 779.6 0 16 222 55 779.6 0 55 223 73 779.6 0 73 224 35 779.6 0 35 225 43 779.6 0 43 226 50 779.6 0 50 227 82 779.6 0 82 228 89 779.6 0 89 229 16 779.6 0 16 230 8 779.6 0 8 231 12 779.6 0 12 232 20 779.6 0 20 233 22 779.6 0 22 234 41 779.6 0 41 235 24 779.6 0 24 236 13 779.6 0 13 237 9 779.6 0 9 238 23 779.6 0 23 239 28 779.6 0 28 240 29 779.6 0 29 241 495 779.6 0 495 242 417 779.6 0 417 243 579 779.6 0 579 244 36 779.6 0 36 245 25 779.6 0 25 246 35 779.6 0 35 247 27 779.6 0 27 248 9 779.6 0 9 249 8 779.6 0 8 250 38 779.6 0 38 251 59 779.6 0 59 252 90 779.6 0 90 253 51 779.6 0 51 254 19 779.6 0 19 255 25 779.6 0 25 256 21 779.6 0 21 257 20 779.6 0 20 258 14 779.6 0 14 259 80 779.6 0 80 260 55 779.6 0 55 261 49 779.6 0 49 262 37 779.6 0 37 263 142 779.6 0 142 264 80 779.6 0 80 265 435 779.6 0 435 266 55 779.6 0 55 267 46 779.6 0 46 268 14 779.6 0 14 269 16 779.6 0 16 270 13 779.6 0 13 271 23 779.6 0 23 272 36 779.6 0 36 273 28 779.6 0 28 274 20 779.6 0 20 275 56 779.6 0 56 276 54 779.6 0 54 277 98 779.6 0 98 278 103 779.6 0 103 279 45 779.6 0 45 280 16 779.6 0 16 281 21 779.6 0 21 282 24 779.6 0 24 283 56 779.6 0 56 284 21 779.6 0 21 285 12 779.6 0 12 286 40 779.6 0 40 287 28 779.6 0 28 288 21 779.6 0 21 289 29 779.6 0 29 290 46 779.6 0 46 291 81 779.6 0 81 292 38 779.6 0 38 293 31 779.6 0 31 294 60 779.6 0 60 295 67 779.6 0 67 296 132 779.6 0 132 297 118 779.6 0 118 298 141 779.6 0 141 299 100 779.6 0 100 300 80 779.6 0 80 301 233 779.6 0 233 Finished in 28.90 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 679,699 Reads with adapters: 152,328 (22.4%) Reads that were too short: 242 (0.0%) Reads written (passing filters): 152,086 (22.4%) Total basepairs processed: 155,346,330 bp Total written (filtered): 41,196,353 bp (26.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 152328 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 43 0.0 3 0 0 39 4 16 1092 0.0 3 0 1017 60 15 17 144739 0.0 3 140290 3930 392 127 18 513 0.0 3 35 372 30 76 19 1497 0.0 3 1434 26 27 10 20 342 0.0 3 2 4 0 336 21 11 0.0 3 1 0 8 2 22 2 0.0 3 0 0 0 2 23 4 0.0 3 4 24 1 0.0 3 1 28 3 0.0 3 3 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 1 36 1 0.0 3 0 0 0 1 37 4 0.0 3 1 0 0 3 39 1 0.0 3 1 47 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 66 21 0.0 3 0 0 1 20 68 3 0.0 3 0 0 0 3 69 6 0.0 3 0 0 0 6 71 2 0.0 3 0 0 0 2 80 7 0.0 3 0 0 0 7 81 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 104 8 0.0 3 0 0 0 8 105 2624 0.0 3 0 0 3 2621 106 12 0.0 3 0 0 0 12 107 7 0.0 3 0 0 0 7 108 1112 0.0 3 0 0 14 1098 109 4 0.0 3 0 0 2 2 118 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 4 0.0 3 0 0 0 4 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 7 0.0 3 0 0 0 7 156 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 167 9 0.0 3 0 0 0 9 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 183 6 0.0 3 0 0 0 6 184 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 197 6 0.0 3 0 0 1 5 202 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 206 125 0.0 3 0 0 0 125 207 51 0.0 3 0 0 0 51 208 5 0.0 3 0 0 0 5 210 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.44 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 152,086 Reads with adapters: 5,090 (3.3%) Reads written (passing filters): 152,086 (100.0%) Total basepairs processed: 41,196,353 bp Total written (filtered): 41,180,874 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5090 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.1% C: 4.7% G: 17.6% T: 11.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4891 2376.3 0 4891 4 195 594.1 0 195 5 2 148.5 0 2 6 1 37.1 0 1 10 1 0.1 1 0 1