This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.32 s (31 us/read; 1.95 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 51,964 (1.6%) Reads that were too short: 11,612 (0.4%) Reads written (passing filters): 40,352 (1.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 9,884,305 bp (1.0%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 51964 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 195 779.6 0 195 7 3286 779.6 0 3286 8 20314 779.6 0 20314 9 26 779.6 0 26 10 3 779.6 0 3 11 13 779.6 0 13 12 8 779.6 0 8 13 8 779.6 0 8 14 10 779.6 0 10 15 3 779.6 0 3 16 4 779.6 0 4 17 9 779.6 0 9 18 10 779.6 0 10 19 9 779.6 0 9 20 65 779.6 0 65 21 14 779.6 0 14 22 17 779.6 0 17 23 9 779.6 0 9 24 3 779.6 0 3 25 5 779.6 0 5 26 11 779.6 0 11 27 31 779.6 0 31 28 11 779.6 0 11 29 1632 779.6 0 1632 30 28 779.6 0 28 31 185 779.6 0 185 32 43 779.6 0 43 33 11 779.6 0 11 34 10 779.6 0 10 35 8 779.6 0 8 36 9 779.6 0 9 37 10 779.6 0 10 38 9 779.6 0 9 39 6 779.6 0 6 40 9 779.6 0 9 41 7 779.6 0 7 42 3 779.6 0 3 43 5 779.6 0 5 44 8 779.6 0 8 45 10 779.6 0 10 46 8 779.6 0 8 47 7 779.6 0 7 48 6 779.6 0 6 49 6 779.6 0 6 50 6 779.6 0 6 51 8 779.6 0 8 52 9 779.6 0 9 53 6 779.6 0 6 54 24 779.6 0 24 55 29 779.6 0 29 56 22 779.6 0 22 57 3 779.6 0 3 58 4 779.6 0 4 59 6 779.6 0 6 60 9 779.6 0 9 61 37 779.6 0 37 62 125 779.6 0 125 63 159 779.6 0 159 64 9 779.6 0 9 65 5 779.6 0 5 66 11 779.6 0 11 67 43 779.6 0 43 68 76 779.6 0 76 69 40 779.6 0 40 70 11 779.6 0 11 71 131 779.6 0 131 72 22 779.6 0 22 73 13 779.6 0 13 74 30 779.6 0 30 75 15 779.6 0 15 76 84 779.6 0 84 77 137 779.6 0 137 78 69 779.6 0 69 79 30 779.6 0 30 80 56 779.6 0 56 81 36 779.6 0 36 82 53 779.6 0 53 83 71 779.6 0 71 84 100 779.6 0 100 85 29 779.6 0 29 86 71 779.6 0 71 87 36 779.6 0 36 88 23 779.6 0 23 89 35 779.6 0 35 90 34 779.6 0 34 91 14 779.6 0 14 92 30 779.6 0 30 93 93 779.6 0 93 94 103 779.6 0 103 95 59 779.6 0 59 96 23 779.6 0 23 97 16 779.6 0 16 98 63 779.6 0 63 99 102 779.6 0 102 100 405 779.6 0 405 101 17 779.6 0 17 102 10 779.6 0 10 103 15 779.6 0 15 104 13 779.6 0 13 105 9 779.6 0 9 106 10 779.6 0 10 107 16 779.6 0 16 108 6 779.6 0 6 109 13 779.6 0 13 110 50 779.6 0 50 111 67 779.6 0 67 112 57 779.6 0 57 113 38 779.6 0 38 114 36 779.6 0 36 115 34 779.6 0 34 116 34 779.6 0 34 117 40 779.6 0 40 118 96 779.6 0 96 119 41 779.6 0 41 120 27 779.6 0 27 121 30 779.6 0 30 122 40 779.6 0 40 123 61 779.6 0 61 124 122 779.6 0 122 125 78 779.6 0 78 126 123 779.6 0 123 127 343 779.6 0 343 128 386 779.6 0 386 129 996 779.6 0 996 130 2208 779.6 0 2208 131 131 779.6 0 131 132 76 779.6 0 76 133 44 779.6 0 44 134 177 779.6 0 177 135 49 779.6 0 49 136 56 779.6 0 56 137 44 779.6 0 44 138 54 779.6 0 54 139 145 779.6 0 145 140 35 779.6 0 35 141 31 779.6 0 31 142 29 779.6 0 29 143 233 779.6 0 233 144 66 779.6 0 66 145 79 779.6 0 79 146 56 779.6 0 56 147 54 779.6 0 54 148 45 779.6 0 45 149 18 779.6 0 18 150 22 779.6 0 22 151 30 779.6 0 30 152 20 779.6 0 20 153 37 779.6 0 37 154 35 779.6 0 35 155 42 779.6 0 42 156 194 779.6 0 194 157 71 779.6 0 71 158 73 779.6 0 73 159 52 779.6 0 52 160 32 779.6 0 32 161 31 779.6 0 31 162 44 779.6 0 44 163 48 779.6 0 48 164 57 779.6 0 57 165 66 779.6 0 66 166 129 779.6 0 129 167 129 779.6 0 129 168 169 779.6 0 169 169 36 779.6 0 36 170 20 779.6 0 20 171 28 779.6 0 28 172 25 779.6 0 25 173 41 779.6 0 41 174 54 779.6 0 54 175 23 779.6 0 23 176 30 779.6 0 30 177 35 779.6 0 35 178 70 779.6 0 70 179 102 779.6 0 102 180 81 779.6 0 81 181 42 779.6 0 42 182 64 779.6 0 64 183 66 779.6 0 66 184 91 779.6 0 91 185 53 779.6 0 53 186 31 779.6 0 31 187 26 779.6 0 26 188 26 779.6 0 26 189 44 779.6 0 44 190 107 779.6 0 107 191 155 779.6 0 155 192 122 779.6 0 122 193 58 779.6 0 58 194 39 779.6 0 39 195 96 779.6 0 96 196 272 779.6 0 272 197 831 779.6 0 831 198 750 779.6 0 750 199 439 779.6 0 439 200 46 779.6 0 46 201 21 779.6 0 21 202 73 779.6 0 73 203 174 779.6 0 174 204 104 779.6 0 104 205 42 779.6 0 42 206 41 779.6 0 41 207 34 779.6 0 34 208 35 779.6 0 35 209 57 779.6 0 57 210 106 779.6 0 106 211 197 779.6 0 197 212 206 779.6 0 206 213 134 779.6 0 134 214 73 779.6 0 73 215 71 779.6 0 71 216 92 779.6 0 92 217 143 779.6 0 143 218 90 779.6 0 90 219 167 779.6 0 167 220 187 779.6 0 187 221 108 779.6 0 108 222 189 779.6 0 189 223 299 779.6 0 299 224 131 779.6 0 131 225 88 779.6 0 88 226 67 779.6 0 67 227 45 779.6 0 45 228 31 779.6 0 31 229 34 779.6 0 34 230 21 779.6 0 21 231 25 779.6 0 25 232 34 779.6 0 34 233 74 779.6 0 74 234 102 779.6 0 102 235 103 779.6 0 103 236 50 779.6 0 50 237 40 779.6 0 40 238 112 779.6 0 112 239 155 779.6 0 155 240 229 779.6 0 229 241 132 779.6 0 132 242 231 779.6 0 231 243 129 779.6 0 129 244 138 779.6 0 138 245 81 779.6 0 81 246 92 779.6 0 92 247 79 779.6 0 79 248 33 779.6 0 33 249 80 779.6 0 80 250 101 779.6 0 101 251 496 779.6 0 496 252 224 779.6 0 224 253 91 779.6 0 91 254 62 779.6 0 62 255 74 779.6 0 74 256 191 779.6 0 191 257 22 779.6 0 22 258 19 779.6 0 19 259 35 779.6 0 35 260 34 779.6 0 34 261 45 779.6 0 45 262 144 779.6 0 144 263 249 779.6 0 249 264 378 779.6 0 378 265 190 779.6 0 190 266 143 779.6 0 143 267 94 779.6 0 94 268 82 779.6 0 82 269 60 779.6 0 60 270 54 779.6 0 54 271 76 779.6 0 76 272 239 779.6 0 239 273 246 779.6 0 246 274 255 779.6 0 255 275 38 779.6 0 38 276 58 779.6 0 58 277 94 779.6 0 94 278 128 779.6 0 128 279 68 779.6 0 68 280 35 779.6 0 35 281 28 779.6 0 28 282 31 779.6 0 31 283 70 779.6 0 70 284 56 779.6 0 56 285 62 779.6 0 62 286 54 779.6 0 54 287 79 779.6 0 79 288 42 779.6 0 42 289 57 779.6 0 57 290 78 779.6 0 78 291 123 779.6 0 123 292 75 779.6 0 75 293 53 779.6 0 53 294 41 779.6 0 41 295 28 779.6 0 28 296 59 779.6 0 59 297 190 779.6 0 190 298 81 779.6 0 81 299 218 779.6 0 218 300 477 779.6 0 477 301 727 779.6 0 727 Finished in 1.93 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 40,352 Reads with adapters: 2,840 (7.0%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 2,798 (6.9%) Total basepairs processed: 9,884,305 bp Total written (filtered): 763,061 bp (7.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2840 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 30 0.0 3 0 28 1 1 17 2672 0.0 3 2597 60 13 2 18 5 0.0 3 0 3 1 1 19 7 0.0 3 0 0 5 2 20 11 0.0 3 6 1 0 4 33 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 63 28 0.0 3 0 0 0 28 65 5 0.0 3 0 0 0 5 66 22 0.0 3 0 0 0 22 71 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 217 23 0.0 3 0 0 0 23 222 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 6 0.0 3 0 0 0 6 283 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 2,798 Reads with adapters: 373 (13.3%) Reads written (passing filters): 2,798 (100.0%) Total basepairs processed: 763,061 bp Total written (filtered): 761,935 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 373 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.2% C: 58.7% G: 9.9% T: 13.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 366 43.7 0 366 4 7 10.9 0 7