This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 96.14 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 877,235 (27.5%) Reads that were too short: 818,466 (25.6%) Reads written (passing filters): 58,769 (1.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 10,015,097 bp (1.0%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 877235 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 69 779.6 0 69 7 2728 779.6 0 2728 8 29 779.6 0 29 9 28 779.6 0 28 10 28 779.6 0 28 11 27 779.6 0 27 12 12 779.6 0 12 13 10 779.6 0 10 14 23 779.6 0 23 15 22 779.6 0 22 16 14 779.6 0 14 17 20 779.6 0 20 18 19 779.6 0 19 19 17 779.6 0 17 20 10 779.6 0 10 21 21 779.6 0 21 22 20 779.6 0 20 23 18 779.6 0 18 24 10 779.6 0 10 25 19 779.6 0 19 26 29 779.6 0 29 27 29 779.6 0 29 28 44 779.6 0 44 29 32 779.6 0 32 30 62 779.6 0 62 31 1014 779.6 0 1014 32 26 779.6 0 26 33 23 779.6 0 23 34 30 779.6 0 30 35 22 779.6 0 22 36 100 779.6 0 100 37 105 779.6 0 105 38 62 779.6 0 62 39 61 779.6 0 61 40 53 779.6 0 53 41 29 779.6 0 29 42 22 779.6 0 22 43 39 779.6 0 39 44 25 779.6 0 25 45 20 779.6 0 20 46 26 779.6 0 26 47 13 779.6 0 13 48 14 779.6 0 14 49 22 779.6 0 22 50 21 779.6 0 21 51 22 779.6 0 22 52 20 779.6 0 20 53 34 779.6 0 34 54 28 779.6 0 28 55 12 779.6 0 12 56 13 779.6 0 13 57 15 779.6 0 15 58 22 779.6 0 22 59 27 779.6 0 27 60 22 779.6 0 22 61 17 779.6 0 17 62 27 779.6 0 27 63 15 779.6 0 15 64 27 779.6 0 27 65 21 779.6 0 21 66 17 779.6 0 17 67 24 779.6 0 24 68 28 779.6 0 28 69 130 779.6 0 130 70 82 779.6 0 82 71 260 779.6 0 260 72 82 779.6 0 82 73 137 779.6 0 137 74 65 779.6 0 65 75 42 779.6 0 42 76 40 779.6 0 40 77 103 779.6 0 103 78 752 779.6 0 752 79 153 779.6 0 153 80 191 779.6 0 191 81 252 779.6 0 252 82 265 779.6 0 265 83 132 779.6 0 132 84 180 779.6 0 180 85 80 779.6 0 80 86 113 779.6 0 113 87 243 779.6 0 243 88 446 779.6 0 446 89 52 779.6 0 52 90 69 779.6 0 69 91 781 779.6 0 781 92 338 779.6 0 338 93 388 779.6 0 388 94 214 779.6 0 214 95 206 779.6 0 206 96 155 779.6 0 155 97 819 779.6 0 819 98 2276 779.6 0 2276 99 661 779.6 0 661 100 169 779.6 0 169 101 445 779.6 0 445 102 96 779.6 0 96 103 110 779.6 0 110 104 433 779.6 0 433 105 122 779.6 0 122 106 78 779.6 0 78 107 507 779.6 0 507 108 562 779.6 0 562 109 85 779.6 0 85 110 250 779.6 0 250 111 435 779.6 0 435 112 278 779.6 0 278 113 238 779.6 0 238 114 222 779.6 0 222 115 195 779.6 0 195 116 343 779.6 0 343 117 342 779.6 0 342 118 102 779.6 0 102 119 754 779.6 0 754 120 402 779.6 0 402 121 396 779.6 0 396 122 181 779.6 0 181 123 273 779.6 0 273 124 331 779.6 0 331 125 405 779.6 0 405 126 150 779.6 0 150 127 87 779.6 0 87 128 301 779.6 0 301 129 481 779.6 0 481 130 365 779.6 0 365 131 278 779.6 0 278 132 494 779.6 0 494 133 440 779.6 0 440 134 520 779.6 0 520 135 210 779.6 0 210 136 201 779.6 0 201 137 235 779.6 0 235 138 282 779.6 0 282 139 1359 779.6 0 1359 140 472 779.6 0 472 141 529 779.6 0 529 142 198 779.6 0 198 143 367 779.6 0 367 144 182 779.6 0 182 145 284 779.6 0 284 146 238 779.6 0 238 147 222 779.6 0 222 148 152 779.6 0 152 149 155 779.6 0 155 150 214 779.6 0 214 151 122 779.6 0 122 152 225 779.6 0 225 153 323 779.6 0 323 154 379 779.6 0 379 155 877 779.6 0 877 156 351 779.6 0 351 157 431 779.6 0 431 158 473 779.6 0 473 159 3282 779.6 0 3282 160 5300 779.6 0 5300 161 3760 779.6 0 3760 162 200 779.6 0 200 163 303 779.6 0 303 164 204 779.6 0 204 165 333 779.6 0 333 166 247 779.6 0 247 167 361 779.6 0 361 168 319 779.6 0 319 169 126 779.6 0 126 170 99 779.6 0 99 171 212 779.6 0 212 172 375 779.6 0 375 173 138 779.6 0 138 174 249 779.6 0 249 175 180 779.6 0 180 176 176 779.6 0 176 177 181 779.6 0 181 178 308 779.6 0 308 179 274 779.6 0 274 180 640 779.6 0 640 181 416 779.6 0 416 182 246 779.6 0 246 183 316 779.6 0 316 184 321 779.6 0 321 185 294 779.6 0 294 186 111 779.6 0 111 187 157 779.6 0 157 188 247 779.6 0 247 189 284 779.6 0 284 190 307 779.6 0 307 191 291 779.6 0 291 192 546 779.6 0 546 193 310 779.6 0 310 194 409 779.6 0 409 195 596 779.6 0 596 196 690 779.6 0 690 197 153 779.6 0 153 198 124 779.6 0 124 199 112 779.6 0 112 200 325 779.6 0 325 201 731 779.6 0 731 202 468 779.6 0 468 203 391 779.6 0 391 204 421 779.6 0 421 205 350 779.6 0 350 206 550 779.6 0 550 207 1006 779.6 0 1006 208 1298 779.6 0 1298 209 887 779.6 0 887 210 676 779.6 0 676 211 624 779.6 0 624 212 586 779.6 0 586 213 389 779.6 0 389 214 397 779.6 0 397 215 342 779.6 0 342 216 302 779.6 0 302 217 258 779.6 0 258 218 1025 779.6 0 1025 219 417 779.6 0 417 220 510 779.6 0 510 221 1274 779.6 0 1274 222 537 779.6 0 537 223 520 779.6 0 520 224 928 779.6 0 928 225 596 779.6 0 596 226 334 779.6 0 334 227 519 779.6 0 519 228 1924 779.6 0 1924 229 331 779.6 0 331 230 253 779.6 0 253 231 390 779.6 0 390 232 1097 779.6 0 1097 233 8324 779.6 0 8324 234 2749 779.6 0 2749 235 655 779.6 0 655 236 506 779.6 0 506 237 980 779.6 0 980 238 2373 779.6 0 2373 239 558 779.6 0 558 240 913 779.6 0 913 241 1044 779.6 0 1044 242 1448 779.6 0 1448 243 1618 779.6 0 1618 244 1228 779.6 0 1228 245 1271 779.6 0 1271 246 644 779.6 0 644 247 588 779.6 0 588 248 986 779.6 0 986 249 1506 779.6 0 1506 250 3086 779.6 0 3086 251 3291 779.6 0 3291 252 4734 779.6 0 4734 253 10121 779.6 0 10121 254 23958 779.6 0 23958 255 167326 779.6 0 167326 256 323878 779.6 0 323878 257 218691 779.6 0 218691 258 3384 779.6 0 3384 259 392 779.6 0 392 260 570 779.6 0 570 261 897 779.6 0 897 262 618 779.6 0 618 263 383 779.6 0 383 264 251 779.6 0 251 265 240 779.6 0 240 266 165 779.6 0 165 267 102 779.6 0 102 268 104 779.6 0 104 269 246 779.6 0 246 270 299 779.6 0 299 271 366 779.6 0 366 272 370 779.6 0 370 273 299 779.6 0 299 274 166 779.6 0 166 275 118 779.6 0 118 276 160 779.6 0 160 277 153 779.6 0 153 278 225 779.6 0 225 279 242 779.6 0 242 280 174 779.6 0 174 281 279 779.6 0 279 282 498 779.6 0 498 283 362 779.6 0 362 284 389 779.6 0 389 285 147 779.6 0 147 286 95 779.6 0 95 287 61 779.6 0 61 288 98 779.6 0 98 289 263 779.6 0 263 290 295 779.6 0 295 291 323 779.6 0 323 292 181 779.6 0 181 293 103 779.6 0 103 294 132 779.6 0 132 295 230 779.6 0 230 296 248 779.6 0 248 297 626 779.6 0 626 298 933 779.6 0 933 299 520 779.6 0 520 300 306 779.6 0 306 301 377 779.6 0 377 Finished in 2.49 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 58,769 Reads with adapters: 2,781 (4.7%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 2,768 (4.7%) Total basepairs processed: 10,015,097 bp Total written (filtered): 757,755 bp (7.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2781 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 17 0.0 3 0 17 20 2728 0.0 4 2681 43 2 2 21 13 0.0 4 3 10 22 1 0.0 4 0 0 1 23 3 0.0 4 0 0 0 3 25 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 2,768 Reads with adapters: 13 (0.5%) Reads written (passing filters): 2,768 (100.0%) Total basepairs processed: 757,755 bp Total written (filtered): 757,613 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 7.7% G: 92.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 10 1 0.0 1 0 1 11 12 0.0 1 0 12