This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.53 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 135,160 (4.2%) Reads that were too short: 32,270 (1.0%) Reads written (passing filters): 102,890 (3.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 26,548,499 bp (2.8%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 135160 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1959 779.6 0 1959 7 64891 779.6 0 64891 8 407 779.6 0 407 9 8817 779.6 0 8817 10 35 779.6 0 35 11 7 779.6 0 7 12 5 779.6 0 5 13 7 779.6 0 7 14 5 779.6 0 5 15 2 779.6 0 2 16 11 779.6 0 11 17 7 779.6 0 7 18 4 779.6 0 4 20 3 779.6 0 3 21 8 779.6 0 8 22 16 779.6 0 16 23 16 779.6 0 16 24 1 779.6 0 1 25 4 779.6 0 4 26 3 779.6 0 3 27 2 779.6 0 2 28 6 779.6 0 6 29 8 779.6 0 8 30 8 779.6 0 8 31 5 779.6 0 5 32 3 779.6 0 3 33 4 779.6 0 4 34 5 779.6 0 5 36 3 779.6 0 3 37 5 779.6 0 5 38 8 779.6 0 8 39 2 779.6 0 2 40 11 779.6 0 11 41 108 779.6 0 108 42 30 779.6 0 30 43 7 779.6 0 7 44 1 779.6 0 1 45 5 779.6 0 5 46 5 779.6 0 5 47 4 779.6 0 4 48 8 779.6 0 8 49 3 779.6 0 3 50 4 779.6 0 4 51 8 779.6 0 8 52 5 779.6 0 5 53 24 779.6 0 24 54 14 779.6 0 14 55 6 779.6 0 6 56 10 779.6 0 10 57 6 779.6 0 6 58 5 779.6 0 5 59 2 779.6 0 2 60 9 779.6 0 9 61 4 779.6 0 4 63 7 779.6 0 7 64 4 779.6 0 4 65 8 779.6 0 8 66 13 779.6 0 13 67 23 779.6 0 23 68 24 779.6 0 24 69 223 779.6 0 223 70 481 779.6 0 481 71 456 779.6 0 456 72 2 779.6 0 2 73 10 779.6 0 10 74 3 779.6 0 3 75 18 779.6 0 18 76 26 779.6 0 26 77 9 779.6 0 9 78 29 779.6 0 29 79 22 779.6 0 22 80 107 779.6 0 107 81 44 779.6 0 44 82 52 779.6 0 52 83 32 779.6 0 32 84 71 779.6 0 71 85 76 779.6 0 76 86 102 779.6 0 102 87 101 779.6 0 101 88 130 779.6 0 130 89 180 779.6 0 180 90 42 779.6 0 42 91 107 779.6 0 107 92 52 779.6 0 52 93 30 779.6 0 30 94 58 779.6 0 58 95 42 779.6 0 42 96 51 779.6 0 51 97 72 779.6 0 72 98 37 779.6 0 37 99 193 779.6 0 193 100 620 779.6 0 620 101 479 779.6 0 479 102 790 779.6 0 790 103 258 779.6 0 258 104 84 779.6 0 84 105 41 779.6 0 41 106 22 779.6 0 22 107 34 779.6 0 34 108 35 779.6 0 35 109 29 779.6 0 29 110 15 779.6 0 15 111 32 779.6 0 32 112 60 779.6 0 60 113 60 779.6 0 60 114 40 779.6 0 40 115 57 779.6 0 57 116 64 779.6 0 64 117 105 779.6 0 105 118 103 779.6 0 103 119 157 779.6 0 157 120 113 779.6 0 113 121 147 779.6 0 147 122 109 779.6 0 109 123 196 779.6 0 196 124 176 779.6 0 176 125 126 779.6 0 126 126 144 779.6 0 144 127 75 779.6 0 75 128 79 779.6 0 79 129 44 779.6 0 44 130 103 779.6 0 103 131 137 779.6 0 137 132 230 779.6 0 230 133 294 779.6 0 294 134 277 779.6 0 277 135 178 779.6 0 178 136 112 779.6 0 112 137 151 779.6 0 151 138 143 779.6 0 143 139 143 779.6 0 143 140 140 779.6 0 140 141 114 779.6 0 114 142 202 779.6 0 202 143 1955 779.6 0 1955 144 570 779.6 0 570 145 150 779.6 0 150 146 118 779.6 0 118 147 134 779.6 0 134 148 261 779.6 0 261 149 178 779.6 0 178 150 194 779.6 0 194 151 171 779.6 0 171 152 150 779.6 0 150 153 123 779.6 0 123 154 115 779.6 0 115 155 129 779.6 0 129 156 190 779.6 0 190 157 239 779.6 0 239 158 578 779.6 0 578 159 673 779.6 0 673 160 619 779.6 0 619 161 390 779.6 0 390 162 362 779.6 0 362 163 227 779.6 0 227 164 200 779.6 0 200 165 145 779.6 0 145 166 145 779.6 0 145 167 158 779.6 0 158 168 242 779.6 0 242 169 251 779.6 0 251 170 374 779.6 0 374 171 182 779.6 0 182 172 249 779.6 0 249 173 96 779.6 0 96 174 108 779.6 0 108 175 144 779.6 0 144 176 1419 779.6 0 1419 177 384 779.6 0 384 178 376 779.6 0 376 179 153 779.6 0 153 180 125 779.6 0 125 181 184 779.6 0 184 182 206 779.6 0 206 183 236 779.6 0 236 184 282 779.6 0 282 185 189 779.6 0 189 186 198 779.6 0 198 187 283 779.6 0 283 188 524 779.6 0 524 189 558 779.6 0 558 190 175 779.6 0 175 191 93 779.6 0 93 192 81 779.6 0 81 193 93 779.6 0 93 194 216 779.6 0 216 195 154 779.6 0 154 196 258 779.6 0 258 197 197 779.6 0 197 198 109 779.6 0 109 199 155 779.6 0 155 200 220 779.6 0 220 201 201 779.6 0 201 202 154 779.6 0 154 203 121 779.6 0 121 204 181 779.6 0 181 205 188 779.6 0 188 206 241 779.6 0 241 207 297 779.6 0 297 208 191 779.6 0 191 209 238 779.6 0 238 210 159 779.6 0 159 211 198 779.6 0 198 212 221 779.6 0 221 213 270 779.6 0 270 214 338 779.6 0 338 215 296 779.6 0 296 216 447 779.6 0 447 217 280 779.6 0 280 218 300 779.6 0 300 219 269 779.6 0 269 220 288 779.6 0 288 221 302 779.6 0 302 222 353 779.6 0 353 223 351 779.6 0 351 224 396 779.6 0 396 225 356 779.6 0 356 226 243 779.6 0 243 227 225 779.6 0 225 228 261 779.6 0 261 229 252 779.6 0 252 230 278 779.6 0 278 231 227 779.6 0 227 232 175 779.6 0 175 233 184 779.6 0 184 234 174 779.6 0 174 235 187 779.6 0 187 236 249 779.6 0 249 237 284 779.6 0 284 238 658 779.6 0 658 239 629 779.6 0 629 240 1222 779.6 0 1222 241 326 779.6 0 326 242 296 779.6 0 296 243 342 779.6 0 342 244 395 779.6 0 395 245 365 779.6 0 365 246 379 779.6 0 379 247 336 779.6 0 336 248 477 779.6 0 477 249 579 779.6 0 579 250 338 779.6 0 338 251 279 779.6 0 279 252 287 779.6 0 287 253 570 779.6 0 570 254 552 779.6 0 552 255 674 779.6 0 674 256 205 779.6 0 205 257 183 779.6 0 183 258 192 779.6 0 192 259 231 779.6 0 231 260 188 779.6 0 188 261 164 779.6 0 164 262 306 779.6 0 306 263 263 779.6 0 263 264 299 779.6 0 299 265 215 779.6 0 215 266 181 779.6 0 181 267 246 779.6 0 246 268 374 779.6 0 374 269 426 779.6 0 426 270 404 779.6 0 404 271 366 779.6 0 366 272 244 779.6 0 244 273 279 779.6 0 279 274 313 779.6 0 313 275 413 779.6 0 413 276 614 779.6 0 614 277 531 779.6 0 531 278 501 779.6 0 501 279 221 779.6 0 221 280 368 779.6 0 368 281 372 779.6 0 372 282 515 779.6 0 515 283 526 779.6 0 526 284 318 779.6 0 318 285 244 779.6 0 244 286 141 779.6 0 141 287 156 779.6 0 156 288 269 779.6 0 269 289 352 779.6 0 352 290 324 779.6 0 324 291 328 779.6 0 328 292 260 779.6 0 260 293 277 779.6 0 277 294 301 779.6 0 301 295 355 779.6 0 355 296 269 779.6 0 269 297 146 779.6 0 146 298 186 779.6 0 186 299 152 779.6 0 152 300 205 779.6 0 205 301 969 779.6 0 969 Finished in 5.06 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 102,890 Reads with adapters: 9,324 (9.1%) Reads that were too short: 69 (0.1%) Reads written (passing filters): 9,255 (9.0%) Total basepairs processed: 26,548,499 bp Total written (filtered): 2,529,129 bp (9.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9324 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 109 0.0 3 0 105 3 1 17 8792 0.0 3 8406 335 37 14 18 21 0.0 3 3 13 2 3 19 8 0.0 3 1 3 3 1 20 213 0.0 3 194 7 0 12 21 1 0.0 3 0 0 0 1 22 6 0.0 3 0 0 0 6 23 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 37 2 0.0 3 0 0 1 1 41 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 21 0.0 3 0 0 0 21 52 23 0.0 3 0 1 0 22 58 3 0.0 3 0 0 0 3 66 2 0.0 3 0 0 0 2 69 3 0.0 3 0 0 0 3 71 8 0.0 3 0 0 0 8 72 6 0.0 3 0 0 0 6 73 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 91 7 0.0 3 0 0 0 7 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 150 4 0.0 3 0 0 0 4 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 0 5 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 25 0.0 3 0 0 1 24 283 5 0.0 3 0 0 0 5 285 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 9,255 Reads with adapters: 675 (7.3%) Reads written (passing filters): 9,255 (100.0%) Total basepairs processed: 2,529,129 bp Total written (filtered): 2,527,030 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 675 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.7% C: 6.2% G: 19.9% T: 13.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 601 144.6 0 601 4 74 36.2 0 74