This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 96.72 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 61,418 (1.9%) Reads that were too short: 22,403 (0.7%) Reads written (passing filters): 39,015 (1.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 7,232,301 bp (0.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 61418 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2670 779.6 0 2670 7 102 779.6 0 102 8 406 779.6 0 406 9 9584 779.6 0 9584 10 24 779.6 0 24 11 9 779.6 0 9 12 2 779.6 0 2 13 6 779.6 0 6 14 1 779.6 0 1 15 9 779.6 0 9 16 7 779.6 0 7 17 5 779.6 0 5 18 8 779.6 0 8 19 1 779.6 0 1 20 6 779.6 0 6 21 11 779.6 0 11 22 11 779.6 0 11 23 3 779.6 0 3 24 10 779.6 0 10 25 6 779.6 0 6 26 4 779.6 0 4 27 6 779.6 0 6 28 14 779.6 0 14 29 14 779.6 0 14 30 92 779.6 0 92 31 4 779.6 0 4 32 10 779.6 0 10 33 2 779.6 0 2 34 4 779.6 0 4 35 5 779.6 0 5 36 6 779.6 0 6 37 17 779.6 0 17 38 8 779.6 0 8 39 7 779.6 0 7 40 8 779.6 0 8 41 5 779.6 0 5 42 8 779.6 0 8 43 5 779.6 0 5 44 6 779.6 0 6 45 2 779.6 0 2 46 5 779.6 0 5 47 10 779.6 0 10 48 11 779.6 0 11 49 4 779.6 0 4 50 6 779.6 0 6 51 8 779.6 0 8 52 8 779.6 0 8 53 8 779.6 0 8 54 13 779.6 0 13 55 12 779.6 0 12 56 5 779.6 0 5 57 6 779.6 0 6 58 8 779.6 0 8 59 12 779.6 0 12 60 3 779.6 0 3 61 10 779.6 0 10 62 6 779.6 0 6 63 7 779.6 0 7 64 13 779.6 0 13 65 16 779.6 0 16 66 22 779.6 0 22 67 13 779.6 0 13 68 9 779.6 0 9 69 90 779.6 0 90 70 15 779.6 0 15 71 12 779.6 0 12 72 7 779.6 0 7 73 8 779.6 0 8 74 13 779.6 0 13 75 14 779.6 0 14 76 9 779.6 0 9 77 17 779.6 0 17 78 11 779.6 0 11 79 72 779.6 0 72 80 151 779.6 0 151 81 648 779.6 0 648 82 12 779.6 0 12 83 8 779.6 0 8 84 15 779.6 0 15 85 7 779.6 0 7 86 8 779.6 0 8 87 11 779.6 0 11 88 5 779.6 0 5 89 5 779.6 0 5 90 11 779.6 0 11 91 7 779.6 0 7 92 4 779.6 0 4 93 12 779.6 0 12 94 6 779.6 0 6 95 12 779.6 0 12 96 29 779.6 0 29 97 99 779.6 0 99 98 10 779.6 0 10 99 13 779.6 0 13 100 13 779.6 0 13 101 11 779.6 0 11 102 12 779.6 0 12 103 36 779.6 0 36 104 24 779.6 0 24 105 6 779.6 0 6 106 7 779.6 0 7 107 29 779.6 0 29 108 100 779.6 0 100 109 13 779.6 0 13 110 18 779.6 0 18 111 12 779.6 0 12 112 14 779.6 0 14 113 26 779.6 0 26 114 20 779.6 0 20 115 44 779.6 0 44 116 20 779.6 0 20 117 72 779.6 0 72 118 117 779.6 0 117 119 91 779.6 0 91 120 11 779.6 0 11 121 15 779.6 0 15 122 16 779.6 0 16 123 11 779.6 0 11 124 20 779.6 0 20 125 13 779.6 0 13 126 10 779.6 0 10 127 36 779.6 0 36 128 70 779.6 0 70 129 39 779.6 0 39 130 56 779.6 0 56 131 72 779.6 0 72 132 77 779.6 0 77 133 119 779.6 0 119 134 25 779.6 0 25 135 29 779.6 0 29 136 28 779.6 0 28 137 88 779.6 0 88 138 20 779.6 0 20 139 17 779.6 0 17 140 24 779.6 0 24 141 27 779.6 0 27 142 26 779.6 0 26 143 22 779.6 0 22 144 13 779.6 0 13 145 34 779.6 0 34 146 38 779.6 0 38 147 66 779.6 0 66 148 21 779.6 0 21 149 18 779.6 0 18 150 18 779.6 0 18 151 16 779.6 0 16 152 25 779.6 0 25 153 19 779.6 0 19 154 23 779.6 0 23 155 30 779.6 0 30 156 183 779.6 0 183 157 49 779.6 0 49 158 79 779.6 0 79 159 23 779.6 0 23 160 40 779.6 0 40 161 77 779.6 0 77 162 69 779.6 0 69 163 89 779.6 0 89 164 108 779.6 0 108 165 191 779.6 0 191 166 93 779.6 0 93 167 108 779.6 0 108 168 111 779.6 0 111 169 92 779.6 0 92 170 93 779.6 0 93 171 92 779.6 0 92 172 83 779.6 0 83 173 181 779.6 0 181 174 105 779.6 0 105 175 175 779.6 0 175 176 362 779.6 0 362 177 1470 779.6 0 1470 178 3834 779.6 0 3834 179 2429 779.6 0 2429 180 7372 779.6 0 7372 181 1542 779.6 0 1542 182 1410 779.6 0 1410 183 254 779.6 0 254 184 77 779.6 0 77 185 48 779.6 0 48 186 42 779.6 0 42 187 57 779.6 0 57 188 66 779.6 0 66 189 17 779.6 0 17 190 17 779.6 0 17 191 15 779.6 0 15 192 27 779.6 0 27 193 41 779.6 0 41 194 127 779.6 0 127 195 278 779.6 0 278 196 156 779.6 0 156 197 276 779.6 0 276 198 305 779.6 0 305 199 51 779.6 0 51 200 56 779.6 0 56 201 104 779.6 0 104 202 180 779.6 0 180 203 186 779.6 0 186 204 44 779.6 0 44 205 18 779.6 0 18 206 15 779.6 0 15 207 23 779.6 0 23 208 13 779.6 0 13 209 16 779.6 0 16 210 23 779.6 0 23 211 11 779.6 0 11 212 14 779.6 0 14 213 15 779.6 0 15 214 26 779.6 0 26 215 70 779.6 0 70 216 137 779.6 0 137 217 112 779.6 0 112 218 26 779.6 0 26 219 36 779.6 0 36 220 90 779.6 0 90 221 91 779.6 0 91 222 44 779.6 0 44 223 87 779.6 0 87 224 130 779.6 0 130 225 108 779.6 0 108 226 42 779.6 0 42 227 54 779.6 0 54 228 46 779.6 0 46 229 53 779.6 0 53 230 42 779.6 0 42 231 54 779.6 0 54 232 62 779.6 0 62 233 95 779.6 0 95 234 1434 779.6 0 1434 235 1268 779.6 0 1268 236 3716 779.6 0 3716 237 59 779.6 0 59 238 52 779.6 0 52 239 726 779.6 0 726 240 272 779.6 0 272 241 498 779.6 0 498 242 96 779.6 0 96 243 97 779.6 0 97 244 24 779.6 0 24 245 36 779.6 0 36 246 44 779.6 0 44 247 41 779.6 0 41 248 42 779.6 0 42 249 78 779.6 0 78 250 38 779.6 0 38 251 23 779.6 0 23 252 42 779.6 0 42 253 78 779.6 0 78 254 49 779.6 0 49 255 83 779.6 0 83 256 79 779.6 0 79 257 82 779.6 0 82 258 170 779.6 0 170 259 519 779.6 0 519 260 1578 779.6 0 1578 261 813 779.6 0 813 262 87 779.6 0 87 263 448 779.6 0 448 264 569 779.6 0 569 265 789 779.6 0 789 266 350 779.6 0 350 267 225 779.6 0 225 268 27 779.6 0 27 269 24 779.6 0 24 270 15 779.6 0 15 271 5 779.6 0 5 272 5 779.6 0 5 273 13 779.6 0 13 274 21 779.6 0 21 275 56 779.6 0 56 276 32 779.6 0 32 277 17 779.6 0 17 278 25 779.6 0 25 279 17 779.6 0 17 280 35 779.6 0 35 281 27 779.6 0 27 282 18 779.6 0 18 283 15 779.6 0 15 284 12 779.6 0 12 285 19 779.6 0 19 286 38 779.6 0 38 287 85 779.6 0 85 288 160 779.6 0 160 289 129 779.6 0 129 290 154 779.6 0 154 291 818 779.6 0 818 292 418 779.6 0 418 293 2433 779.6 0 2433 294 588 779.6 0 588 295 148 779.6 0 148 296 126 779.6 0 126 297 134 779.6 0 134 298 44 779.6 0 44 299 97 779.6 0 97 300 92 779.6 0 92 301 88 779.6 0 88 Finished in 1.71 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 39,015 Reads with adapters: 10,108 (25.9%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 10,105 (25.9%) Total basepairs processed: 7,232,301 bp Total written (filtered): 2,748,589 bp (38.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10108 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 9 0.0 3 0 0 7 1 1 19 98 0.0 3 0 97 1 20 9961 0.0 4 9844 109 4 3 1 21 17 0.0 4 3 14 22 3 0.0 4 0 0 3 23 4 0.0 4 1 0 0 3 24 4 0.0 4 1 0 0 0 3 28 1 0.0 4 1 29 2 0.0 4 0 0 0 0 2 45 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 156 2 0.0 4 0 0 0 0 2 169 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 10,105 Reads with adapters: 122 (1.2%) Reads written (passing filters): 10,105 (100.0%) Total basepairs processed: 2,748,589 bp Total written (filtered): 2,745,742 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 122 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 7.4% G: 86.9% T: 3.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 39.5 0 1 6 1 2.5 0 1 9 12 0.0 0 1 11 10 53 0.0 1 0 53 11 45 0.0 1 0 45 18 2 0.0 1 1 1 19 1 0.0 1 0 1 223 5 0.0 1 5 267 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.