This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.81 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 77,023 (2.4%) Reads that were too short: 26,023 (0.8%) Reads written (passing filters): 51,000 (1.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 10,939,347 bp (1.1%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 77023 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 256 779.6 0 256 7 14428 779.6 0 14428 8 4651 779.6 0 4651 9 17 779.6 0 17 10 16 779.6 0 16 11 189 779.6 0 189 12 12 779.6 0 12 13 8 779.6 0 8 14 13 779.6 0 13 15 12 779.6 0 12 16 6 779.6 0 6 17 9 779.6 0 9 18 7 779.6 0 7 19 11 779.6 0 11 20 7 779.6 0 7 21 8 779.6 0 8 22 9 779.6 0 9 23 9 779.6 0 9 24 8 779.6 0 8 25 9 779.6 0 9 26 13 779.6 0 13 27 10 779.6 0 10 28 12 779.6 0 12 29 10 779.6 0 10 30 53 779.6 0 53 31 101 779.6 0 101 32 32 779.6 0 32 33 9 779.6 0 9 34 30 779.6 0 30 35 17 779.6 0 17 36 24 779.6 0 24 37 44 779.6 0 44 38 20 779.6 0 20 39 15 779.6 0 15 40 42 779.6 0 42 41 20 779.6 0 20 42 28 779.6 0 28 43 30 779.6 0 30 44 39 779.6 0 39 45 29 779.6 0 29 46 28 779.6 0 28 47 41 779.6 0 41 48 46 779.6 0 46 49 72 779.6 0 72 50 899 779.6 0 899 51 1241 779.6 0 1241 52 1758 779.6 0 1758 53 21 779.6 0 21 54 19 779.6 0 19 55 9 779.6 0 9 56 48 779.6 0 48 57 20 779.6 0 20 58 15 779.6 0 15 59 21 779.6 0 21 60 45 779.6 0 45 61 43 779.6 0 43 62 18 779.6 0 18 63 30 779.6 0 30 64 39 779.6 0 39 65 32 779.6 0 32 66 21 779.6 0 21 67 40 779.6 0 40 68 43 779.6 0 43 69 66 779.6 0 66 70 117 779.6 0 117 71 47 779.6 0 47 72 151 779.6 0 151 73 101 779.6 0 101 74 2134 779.6 0 2134 75 2043 779.6 0 2043 76 312 779.6 0 312 77 15 779.6 0 15 78 36 779.6 0 36 79 22 779.6 0 22 80 75 779.6 0 75 81 31 779.6 0 31 82 44 779.6 0 44 83 38 779.6 0 38 84 38 779.6 0 38 85 96 779.6 0 96 86 48 779.6 0 48 87 64 779.6 0 64 88 25 779.6 0 25 89 17 779.6 0 17 90 20 779.6 0 20 91 18 779.6 0 18 92 34 779.6 0 34 93 21 779.6 0 21 94 39 779.6 0 39 95 36 779.6 0 36 96 22 779.6 0 22 97 23 779.6 0 23 98 17 779.6 0 17 99 8 779.6 0 8 100 7 779.6 0 7 101 10 779.6 0 10 102 25 779.6 0 25 103 19 779.6 0 19 104 15 779.6 0 15 105 25 779.6 0 25 106 16 779.6 0 16 107 9 779.6 0 9 108 8 779.6 0 8 109 17 779.6 0 17 110 9 779.6 0 9 111 16 779.6 0 16 112 36 779.6 0 36 113 26 779.6 0 26 114 438 779.6 0 438 115 96 779.6 0 96 116 70 779.6 0 70 117 27 779.6 0 27 118 49 779.6 0 49 119 49 779.6 0 49 120 47 779.6 0 47 121 40 779.6 0 40 122 45 779.6 0 45 123 79 779.6 0 79 124 64 779.6 0 64 125 408 779.6 0 408 126 245 779.6 0 245 127 929 779.6 0 929 128 40 779.6 0 40 129 49 779.6 0 49 130 133 779.6 0 133 131 47 779.6 0 47 132 83 779.6 0 83 133 38 779.6 0 38 134 54 779.6 0 54 135 297 779.6 0 297 136 232 779.6 0 232 137 128 779.6 0 128 138 49 779.6 0 49 139 33 779.6 0 33 140 50 779.6 0 50 141 33 779.6 0 33 142 53 779.6 0 53 143 41 779.6 0 41 144 92 779.6 0 92 145 39 779.6 0 39 146 135 779.6 0 135 147 48 779.6 0 48 148 38 779.6 0 38 149 24 779.6 0 24 150 51 779.6 0 51 151 48 779.6 0 48 152 22 779.6 0 22 153 20 779.6 0 20 154 28 779.6 0 28 155 31 779.6 0 31 156 36 779.6 0 36 157 89 779.6 0 89 158 43 779.6 0 43 159 48 779.6 0 48 160 50 779.6 0 50 161 61 779.6 0 61 162 44 779.6 0 44 163 70 779.6 0 70 164 1265 779.6 0 1265 165 929 779.6 0 929 166 504 779.6 0 504 167 50 779.6 0 50 168 41 779.6 0 41 169 69 779.6 0 69 170 60 779.6 0 60 171 67 779.6 0 67 172 26 779.6 0 26 173 32 779.6 0 32 174 27 779.6 0 27 175 74 779.6 0 74 176 42 779.6 0 42 177 38 779.6 0 38 178 23 779.6 0 23 179 29 779.6 0 29 180 41 779.6 0 41 181 53 779.6 0 53 182 82 779.6 0 82 183 53 779.6 0 53 184 47 779.6 0 47 185 64 779.6 0 64 186 93 779.6 0 93 187 105 779.6 0 105 188 36 779.6 0 36 189 38 779.6 0 38 190 35 779.6 0 35 191 126 779.6 0 126 192 215 779.6 0 215 193 1908 779.6 0 1908 194 177 779.6 0 177 195 200 779.6 0 200 196 736 779.6 0 736 197 1143 779.6 0 1143 198 4162 779.6 0 4162 199 2185 779.6 0 2185 200 277 779.6 0 277 201 177 779.6 0 177 202 29 779.6 0 29 203 25 779.6 0 25 204 64 779.6 0 64 205 86 779.6 0 86 206 67 779.6 0 67 207 76 779.6 0 76 208 51 779.6 0 51 209 48 779.6 0 48 210 63 779.6 0 63 211 108 779.6 0 108 212 105 779.6 0 105 213 170 779.6 0 170 214 97 779.6 0 97 215 110 779.6 0 110 216 187 779.6 0 187 217 327 779.6 0 327 218 1335 779.6 0 1335 219 568 779.6 0 568 220 130 779.6 0 130 221 166 779.6 0 166 222 44 779.6 0 44 223 25 779.6 0 25 224 23 779.6 0 23 225 50 779.6 0 50 226 59 779.6 0 59 227 58 779.6 0 58 228 52 779.6 0 52 229 84 779.6 0 84 230 61 779.6 0 61 231 68 779.6 0 68 232 45 779.6 0 45 233 40 779.6 0 40 234 47 779.6 0 47 235 74 779.6 0 74 236 114 779.6 0 114 237 200 779.6 0 200 238 194 779.6 0 194 239 501 779.6 0 501 240 689 779.6 0 689 241 176 779.6 0 176 242 39 779.6 0 39 243 25 779.6 0 25 244 40 779.6 0 40 245 80 779.6 0 80 246 317 779.6 0 317 247 2538 779.6 0 2538 248 8418 779.6 0 8418 249 2905 779.6 0 2905 250 791 779.6 0 791 251 147 779.6 0 147 252 16 779.6 0 16 253 17 779.6 0 17 254 37 779.6 0 37 255 72 779.6 0 72 256 114 779.6 0 114 257 121 779.6 0 121 258 207 779.6 0 207 259 118 779.6 0 118 260 60 779.6 0 60 261 21 779.6 0 21 262 15 779.6 0 15 263 18 779.6 0 18 264 14 779.6 0 14 265 30 779.6 0 30 266 41 779.6 0 41 267 63 779.6 0 63 268 74 779.6 0 74 269 69 779.6 0 69 270 90 779.6 0 90 271 85 779.6 0 85 272 33 779.6 0 33 273 29 779.6 0 29 274 52 779.6 0 52 275 60 779.6 0 60 276 62 779.6 0 62 277 66 779.6 0 66 278 49 779.6 0 49 279 62 779.6 0 62 280 158 779.6 0 158 281 207 779.6 0 207 282 128 779.6 0 128 283 112 779.6 0 112 284 153 779.6 0 153 285 89 779.6 0 89 286 76 779.6 0 76 287 116 779.6 0 116 288 59 779.6 0 59 289 93 779.6 0 93 290 150 779.6 0 150 291 247 779.6 0 247 292 288 779.6 0 288 293 77 779.6 0 77 294 112 779.6 0 112 295 84 779.6 0 84 296 99 779.6 0 99 297 89 779.6 0 89 298 93 779.6 0 93 299 66 779.6 0 66 300 73 779.6 0 73 301 43 779.6 0 43 Finished in 2.21 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 51,000 Reads with adapters: 14,801 (29.0%) Reads that were too short: 113 (0.2%) Reads written (passing filters): 14,688 (28.8%) Total basepairs processed: 10,939,347 bp Total written (filtered): 4,034,449 bp (36.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14801 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 2 2 16 99 0.0 3 0 82 7 10 17 14267 0.0 3 13742 444 65 16 18 46 0.0 3 2 25 6 13 19 22 0.0 3 0 0 15 7 20 36 0.0 3 0 0 0 36 27 32 0.0 3 0 0 0 32 28 1 0.0 3 0 0 0 1 30 8 0.0 3 0 0 0 8 34 2 0.0 3 0 0 0 2 35 4 0.0 3 0 0 0 4 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 46 12 0.0 3 0 0 0 12 49 4 0.0 3 0 0 0 4 50 2 0.0 3 0 0 0 2 53 4 0.0 3 0 0 0 4 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 87 33 0.0 3 0 0 0 33 88 3 0.0 3 0 0 0 3 90 32 0.0 3 0 0 1 31 91 3 0.0 3 0 0 1 2 96 7 0.0 3 0 0 1 6 97 12 0.0 3 0 0 0 12 98 2 0.0 3 0 0 0 2 100 7 0.0 3 0 0 0 7 103 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 31 0.0 3 0 0 0 31 118 6 0.0 3 0 0 0 6 119 1 0.0 3 0 0 0 1 120 61 0.0 3 0 1 2 58 121 4 0.0 3 0 0 0 4 135 2 0.0 3 0 0 0 2 141 6 0.0 3 0 0 0 6 143 1 0.0 3 0 0 0 1 144 5 0.0 3 0 0 0 5 145 1 0.0 3 0 0 1 158 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 1 1 161 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 1 1 219 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 250 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 14,688 Reads with adapters: 814 (5.5%) Reads written (passing filters): 14,688 (100.0%) Total basepairs processed: 4,034,449 bp Total written (filtered): 4,031,972 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 814 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.9% C: 50.9% G: 10.9% T: 15.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 781 229.5 0 781 4 32 57.4 0 32 6 1 3.6 0 1