This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.85 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 290,479 (9.1%) Reads that were too short: 81,367 (2.5%) Reads written (passing filters): 209,112 (6.5%) Total basepairs processed: 961,105,341 bp Total written (filtered): 56,781,044 bp (5.9%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 290479 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1852 779.6 0 1852 7 113243 779.6 0 113243 8 1273 779.6 0 1273 9 56887 779.6 0 56887 10 107 779.6 0 107 11 12 779.6 0 12 12 9 779.6 0 9 13 8 779.6 0 8 14 8 779.6 0 8 15 70 779.6 0 70 16 28 779.6 0 28 17 30 779.6 0 30 18 13 779.6 0 13 19 15 779.6 0 15 20 9 779.6 0 9 21 6 779.6 0 6 22 15 779.6 0 15 23 6 779.6 0 6 24 15 779.6 0 15 25 10 779.6 0 10 26 7 779.6 0 7 27 16 779.6 0 16 28 9 779.6 0 9 29 8 779.6 0 8 30 6 779.6 0 6 31 18 779.6 0 18 32 8 779.6 0 8 33 11 779.6 0 11 34 13 779.6 0 13 35 11 779.6 0 11 36 7 779.6 0 7 37 9 779.6 0 9 38 28 779.6 0 28 39 29 779.6 0 29 40 22 779.6 0 22 41 14 779.6 0 14 42 6 779.6 0 6 43 12 779.6 0 12 44 13 779.6 0 13 45 12 779.6 0 12 46 16 779.6 0 16 47 10 779.6 0 10 48 14 779.6 0 14 49 9 779.6 0 9 50 20 779.6 0 20 51 29 779.6 0 29 52 12 779.6 0 12 53 25 779.6 0 25 54 19 779.6 0 19 55 44 779.6 0 44 56 44 779.6 0 44 57 317 779.6 0 317 58 535 779.6 0 535 59 1030 779.6 0 1030 60 8 779.6 0 8 61 10 779.6 0 10 62 10 779.6 0 10 63 15 779.6 0 15 64 13 779.6 0 13 65 14 779.6 0 14 66 6 779.6 0 6 67 5 779.6 0 5 68 9 779.6 0 9 69 9 779.6 0 9 70 11 779.6 0 11 71 5 779.6 0 5 72 34 779.6 0 34 73 24 779.6 0 24 74 30 779.6 0 30 75 26 779.6 0 26 76 36 779.6 0 36 77 42 779.6 0 42 78 65 779.6 0 65 79 77 779.6 0 77 80 147 779.6 0 147 81 67 779.6 0 67 82 118 779.6 0 118 83 113 779.6 0 113 84 206 779.6 0 206 85 151 779.6 0 151 86 150 779.6 0 150 87 45 779.6 0 45 88 485 779.6 0 485 89 264 779.6 0 264 90 570 779.6 0 570 91 281 779.6 0 281 92 182 779.6 0 182 93 71 779.6 0 71 94 145 779.6 0 145 95 171 779.6 0 171 96 63 779.6 0 63 97 25 779.6 0 25 98 47 779.6 0 47 99 78 779.6 0 78 100 277 779.6 0 277 101 341 779.6 0 341 102 119 779.6 0 119 103 66 779.6 0 66 104 63 779.6 0 63 105 344 779.6 0 344 106 321 779.6 0 321 107 122 779.6 0 122 108 40 779.6 0 40 109 71 779.6 0 71 110 107 779.6 0 107 111 111 779.6 0 111 112 83 779.6 0 83 113 91 779.6 0 91 114 100 779.6 0 100 115 100 779.6 0 100 116 85 779.6 0 85 117 148 779.6 0 148 118 91 779.6 0 91 119 86 779.6 0 86 120 148 779.6 0 148 121 233 779.6 0 233 122 565 779.6 0 565 123 2405 779.6 0 2405 124 714 779.6 0 714 125 84 779.6 0 84 126 135 779.6 0 135 127 184 779.6 0 184 128 70 779.6 0 70 129 128 779.6 0 128 130 216 779.6 0 216 131 217 779.6 0 217 132 195 779.6 0 195 133 84 779.6 0 84 134 230 779.6 0 230 135 204 779.6 0 204 136 311 779.6 0 311 137 330 779.6 0 330 138 191 779.6 0 191 139 154 779.6 0 154 140 217 779.6 0 217 141 885 779.6 0 885 142 454 779.6 0 454 143 353 779.6 0 353 144 94 779.6 0 94 145 164 779.6 0 164 146 181 779.6 0 181 147 572 779.6 0 572 148 540 779.6 0 540 149 436 779.6 0 436 150 282 779.6 0 282 151 2243 779.6 0 2243 152 652 779.6 0 652 153 1753 779.6 0 1753 154 306 779.6 0 306 155 257 779.6 0 257 156 215 779.6 0 215 157 773 779.6 0 773 158 617 779.6 0 617 159 581 779.6 0 581 160 195 779.6 0 195 161 251 779.6 0 251 162 301 779.6 0 301 163 298 779.6 0 298 164 169 779.6 0 169 165 162 779.6 0 162 166 186 779.6 0 186 167 186 779.6 0 186 168 216 779.6 0 216 169 306 779.6 0 306 170 157 779.6 0 157 171 118 779.6 0 118 172 55 779.6 0 55 173 80 779.6 0 80 174 232 779.6 0 232 175 151 779.6 0 151 176 124 779.6 0 124 177 107 779.6 0 107 178 73 779.6 0 73 179 105 779.6 0 105 180 181 779.6 0 181 181 185 779.6 0 185 182 404 779.6 0 404 183 615 779.6 0 615 184 485 779.6 0 485 185 304 779.6 0 304 186 341 779.6 0 341 187 300 779.6 0 300 188 111 779.6 0 111 189 74 779.6 0 74 190 94 779.6 0 94 191 109 779.6 0 109 192 191 779.6 0 191 193 166 779.6 0 166 194 119 779.6 0 119 195 108 779.6 0 108 196 178 779.6 0 178 197 153 779.6 0 153 198 278 779.6 0 278 199 135 779.6 0 135 200 68 779.6 0 68 201 75 779.6 0 75 202 123 779.6 0 123 203 275 779.6 0 275 204 357 779.6 0 357 205 299 779.6 0 299 206 229 779.6 0 229 207 162 779.6 0 162 208 429 779.6 0 429 209 698 779.6 0 698 210 858 779.6 0 858 211 3476 779.6 0 3476 212 806 779.6 0 806 213 7450 779.6 0 7450 214 7030 779.6 0 7030 215 22418 779.6 0 22418 216 547 779.6 0 547 217 306 779.6 0 306 218 294 779.6 0 294 219 197 779.6 0 197 220 124 779.6 0 124 221 588 779.6 0 588 222 5489 779.6 0 5489 223 3094 779.6 0 3094 224 384 779.6 0 384 225 217 779.6 0 217 226 305 779.6 0 305 227 194 779.6 0 194 228 184 779.6 0 184 229 206 779.6 0 206 230 89 779.6 0 89 231 90 779.6 0 90 232 106 779.6 0 106 233 172 779.6 0 172 234 226 779.6 0 226 235 264 779.6 0 264 236 195 779.6 0 195 237 90 779.6 0 90 238 65 779.6 0 65 239 172 779.6 0 172 240 54 779.6 0 54 241 65 779.6 0 65 242 170 779.6 0 170 243 607 779.6 0 607 244 937 779.6 0 937 245 508 779.6 0 508 246 196 779.6 0 196 247 128 779.6 0 128 248 88 779.6 0 88 249 62 779.6 0 62 250 37 779.6 0 37 251 101 779.6 0 101 252 270 779.6 0 270 253 475 779.6 0 475 254 301 779.6 0 301 255 303 779.6 0 303 256 719 779.6 0 719 257 2009 779.6 0 2009 258 1910 779.6 0 1910 259 858 779.6 0 858 260 123 779.6 0 123 261 114 779.6 0 114 262 70 779.6 0 70 263 219 779.6 0 219 264 396 779.6 0 396 265 715 779.6 0 715 266 471 779.6 0 471 267 603 779.6 0 603 268 269 779.6 0 269 269 108 779.6 0 108 270 142 779.6 0 142 271 425 779.6 0 425 272 598 779.6 0 598 273 417 779.6 0 417 274 250 779.6 0 250 275 213 779.6 0 213 276 97 779.6 0 97 277 165 779.6 0 165 278 294 779.6 0 294 279 812 779.6 0 812 280 513 779.6 0 513 281 233 779.6 0 233 282 173 779.6 0 173 283 241 779.6 0 241 284 406 779.6 0 406 285 310 779.6 0 310 286 200 779.6 0 200 287 227 779.6 0 227 288 297 779.6 0 297 289 236 779.6 0 236 290 174 779.6 0 174 291 483 779.6 0 483 292 444 779.6 0 444 293 311 779.6 0 311 294 279 779.6 0 279 295 209 779.6 0 209 296 163 779.6 0 163 297 194 779.6 0 194 298 120 779.6 0 120 299 611 779.6 0 611 300 813 779.6 0 813 301 223 779.6 0 223 Finished in 10.27 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 209,112 Reads with adapters: 58,850 (28.1%) Reads that were too short: 151 (0.1%) Reads written (passing filters): 58,699 (28.1%) Total basepairs processed: 56,781,044 bp Total written (filtered): 16,138,098 bp (28.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58850 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 18 0.0 3 0 0 11 7 16 604 0.0 3 0 575 27 2 17 57595 0.0 3 55750 1633 161 51 18 188 0.0 3 7 132 8 41 19 31 0.0 3 0 0 18 13 20 212 0.0 3 0 0 0 212 21 1 0.0 3 0 0 0 1 31 2 0.0 3 2 32 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 77 11 0.0 3 0 0 0 11 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 160 11 0.0 3 0 0 0 11 164 5 0.0 3 0 0 0 5 166 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 1 216 1 0.0 3 0 0 0 1 217 32 0.0 3 0 0 2 30 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 23 0.0 3 0 0 0 23 282 53 0.0 3 0 0 0 53 283 12 0.0 3 0 0 1 11 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 3 0.0 3 0 0 0 3 290 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.71 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 58,699 Reads with adapters: 11,350 (19.3%) Reads written (passing filters): 58,699 (100.0%) Total basepairs processed: 16,138,098 bp Total written (filtered): 16,103,968 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 11350 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.0% C: 5.1% G: 2.6% T: 4.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11282 917.2 0 11282 4 65 229.3 0 65 5 1 57.3 0 1 8 1 0.9 0 1 11 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.