This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 98.63 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 82,208 (2.6%) Reads that were too short: 5,761 (0.2%) Reads written (passing filters): 76,447 (2.4%) Total basepairs processed: 961,105,341 bp Total written (filtered): 21,268,176 bp (2.2%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 82208 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 622 779.6 0 622 7 851 779.6 0 851 8 2832 779.6 0 2832 9 59094 779.6 0 59094 10 104 779.6 0 104 11 14 779.6 0 14 12 8 779.6 0 8 13 5 779.6 0 5 14 16 779.6 0 16 15 4 779.6 0 4 16 8 779.6 0 8 17 12 779.6 0 12 18 11 779.6 0 11 19 7 779.6 0 7 20 7 779.6 0 7 21 8 779.6 0 8 22 8 779.6 0 8 23 7 779.6 0 7 24 7 779.6 0 7 25 13 779.6 0 13 26 10 779.6 0 10 27 7 779.6 0 7 28 12 779.6 0 12 29 11 779.6 0 11 30 18 779.6 0 18 31 53 779.6 0 53 32 9 779.6 0 9 33 6 779.6 0 6 34 12 779.6 0 12 35 11 779.6 0 11 36 9 779.6 0 9 37 10 779.6 0 10 38 8 779.6 0 8 39 12 779.6 0 12 40 10 779.6 0 10 41 7 779.6 0 7 42 8 779.6 0 8 43 9 779.6 0 9 44 8 779.6 0 8 45 6 779.6 0 6 46 8 779.6 0 8 47 3 779.6 0 3 48 7 779.6 0 7 49 17 779.6 0 17 50 18 779.6 0 18 51 9 779.6 0 9 52 8 779.6 0 8 53 103 779.6 0 103 54 4625 779.6 0 4625 55 273 779.6 0 273 56 56 779.6 0 56 57 6 779.6 0 6 58 16 779.6 0 16 59 12 779.6 0 12 60 12 779.6 0 12 61 14 779.6 0 14 62 10 779.6 0 10 63 12 779.6 0 12 64 20 779.6 0 20 65 16 779.6 0 16 66 7 779.6 0 7 67 28 779.6 0 28 68 34 779.6 0 34 69 52 779.6 0 52 70 26 779.6 0 26 71 41 779.6 0 41 72 105 779.6 0 105 73 1044 779.6 0 1044 74 882 779.6 0 882 75 882 779.6 0 882 76 10 779.6 0 10 77 4 779.6 0 4 78 23 779.6 0 23 79 13 779.6 0 13 80 15 779.6 0 15 81 9 779.6 0 9 82 8 779.6 0 8 83 7 779.6 0 7 84 14 779.6 0 14 85 15 779.6 0 15 86 17 779.6 0 17 87 9 779.6 0 9 88 16 779.6 0 16 89 9 779.6 0 9 90 5 779.6 0 5 91 11 779.6 0 11 92 4 779.6 0 4 93 11 779.6 0 11 94 4 779.6 0 4 95 7 779.6 0 7 96 8 779.6 0 8 97 6 779.6 0 6 98 9 779.6 0 9 99 8 779.6 0 8 100 13 779.6 0 13 101 14 779.6 0 14 102 10 779.6 0 10 103 9 779.6 0 9 104 6 779.6 0 6 105 15 779.6 0 15 106 84 779.6 0 84 107 14 779.6 0 14 108 7 779.6 0 7 109 42 779.6 0 42 110 21 779.6 0 21 111 7 779.6 0 7 112 11 779.6 0 11 113 10 779.6 0 10 114 10 779.6 0 10 115 4 779.6 0 4 116 14 779.6 0 14 117 5 779.6 0 5 118 10 779.6 0 10 119 8 779.6 0 8 120 15 779.6 0 15 121 14 779.6 0 14 122 14 779.6 0 14 123 13 779.6 0 13 124 9 779.6 0 9 125 9 779.6 0 9 126 8 779.6 0 8 127 9 779.6 0 9 128 14 779.6 0 14 129 26 779.6 0 26 130 21 779.6 0 21 131 20 779.6 0 20 132 23 779.6 0 23 133 13 779.6 0 13 134 18 779.6 0 18 135 98 779.6 0 98 136 71 779.6 0 71 137 93 779.6 0 93 138 108 779.6 0 108 139 8 779.6 0 8 140 15 779.6 0 15 141 7 779.6 0 7 142 26 779.6 0 26 143 12 779.6 0 12 144 9 779.6 0 9 145 29 779.6 0 29 146 114 779.6 0 114 147 222 779.6 0 222 148 107 779.6 0 107 149 15 779.6 0 15 150 14 779.6 0 14 151 23 779.6 0 23 152 12 779.6 0 12 153 18 779.6 0 18 154 10 779.6 0 10 155 26 779.6 0 26 156 21 779.6 0 21 157 45 779.6 0 45 158 103 779.6 0 103 159 145 779.6 0 145 160 247 779.6 0 247 161 18 779.6 0 18 162 26 779.6 0 26 163 53 779.6 0 53 164 7 779.6 0 7 165 7 779.6 0 7 166 18 779.6 0 18 167 30 779.6 0 30 168 26 779.6 0 26 169 56 779.6 0 56 170 35 779.6 0 35 171 85 779.6 0 85 172 29 779.6 0 29 173 31 779.6 0 31 174 215 779.6 0 215 175 65 779.6 0 65 176 66 779.6 0 66 177 10 779.6 0 10 178 15 779.6 0 15 179 19 779.6 0 19 180 137 779.6 0 137 181 88 779.6 0 88 182 41 779.6 0 41 183 32 779.6 0 32 184 18 779.6 0 18 185 6 779.6 0 6 186 42 779.6 0 42 187 168 779.6 0 168 188 173 779.6 0 173 189 100 779.6 0 100 190 6 779.6 0 6 191 14 779.6 0 14 192 13 779.6 0 13 193 12 779.6 0 12 194 136 779.6 0 136 195 14 779.6 0 14 196 4 779.6 0 4 197 5 779.6 0 5 198 7 779.6 0 7 199 4 779.6 0 4 200 9 779.6 0 9 201 7 779.6 0 7 202 9 779.6 0 9 203 16 779.6 0 16 204 50 779.6 0 50 205 27 779.6 0 27 206 13 779.6 0 13 207 13 779.6 0 13 208 13 779.6 0 13 209 15 779.6 0 15 210 7 779.6 0 7 211 13 779.6 0 13 212 3 779.6 0 3 213 10 779.6 0 10 214 20 779.6 0 20 215 23 779.6 0 23 216 22 779.6 0 22 217 14 779.6 0 14 218 14 779.6 0 14 219 9 779.6 0 9 220 9 779.6 0 9 221 11 779.6 0 11 222 12 779.6 0 12 223 16 779.6 0 16 224 16 779.6 0 16 225 16 779.6 0 16 226 19 779.6 0 19 227 46 779.6 0 46 228 117 779.6 0 117 229 34 779.6 0 34 230 14 779.6 0 14 231 25 779.6 0 25 232 16 779.6 0 16 233 22 779.6 0 22 234 9 779.6 0 9 235 10 779.6 0 10 236 12 779.6 0 12 237 20 779.6 0 20 238 41 779.6 0 41 239 16 779.6 0 16 240 19 779.6 0 19 241 19 779.6 0 19 242 12 779.6 0 12 243 16 779.6 0 16 244 24 779.6 0 24 245 17 779.6 0 17 246 11 779.6 0 11 247 30 779.6 0 30 248 19 779.6 0 19 249 35 779.6 0 35 250 31 779.6 0 31 251 100 779.6 0 100 252 296 779.6 0 296 253 2982 779.6 0 2982 254 67 779.6 0 67 255 36 779.6 0 36 256 13 779.6 0 13 257 27 779.6 0 27 258 11 779.6 0 11 259 13 779.6 0 13 260 27 779.6 0 27 261 23 779.6 0 23 262 44 779.6 0 44 263 62 779.6 0 62 264 30 779.6 0 30 265 2 779.6 0 2 266 8 779.6 0 8 267 13 779.6 0 13 268 9 779.6 0 9 269 10 779.6 0 10 270 7 779.6 0 7 271 8 779.6 0 8 272 13 779.6 0 13 273 11 779.6 0 11 274 15 779.6 0 15 275 21 779.6 0 21 276 12 779.6 0 12 277 14 779.6 0 14 278 32 779.6 0 32 279 24 779.6 0 24 280 17 779.6 0 17 281 94 779.6 0 94 282 45 779.6 0 45 283 182 779.6 0 182 284 15 779.6 0 15 285 75 779.6 0 75 286 12 779.6 0 12 287 10 779.6 0 10 288 10 779.6 0 10 289 10 779.6 0 10 290 9 779.6 0 9 291 11 779.6 0 11 292 18 779.6 0 18 293 16 779.6 0 16 294 41 779.6 0 41 295 73 779.6 0 73 296 12 779.6 0 12 297 24 779.6 0 24 298 18 779.6 0 18 299 28 779.6 0 28 300 72 779.6 0 72 301 34 779.6 0 34 Finished in 3.74 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 76,447 Reads with adapters: 63,252 (82.7%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 63,252 (82.7%) Total basepairs processed: 21,268,176 bp Total written (filtered): 17,210,353 bp (80.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63252 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 15 0.0 3 0 0 0 4 11 18 43 0.0 3 0 0 42 1 19 860 0.0 3 0 831 23 4 2 20 62069 0.0 4 61104 937 25 2 1 21 145 0.0 4 32 111 2 22 10 0.0 4 1 1 8 23 95 0.0 4 73 1 0 20 1 24 3 0.0 4 1 0 0 0 2 25 1 0.0 4 1 26 1 0.0 4 1 28 1 0.0 4 1 29 2 0.0 4 1 0 0 1 30 1 0.0 4 0 1 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 69 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.64 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 63,252 Reads with adapters: 989 (1.6%) Reads written (passing filters): 63,252 (100.0%) Total basepairs processed: 17,210,353 bp Total written (filtered): 17,193,862 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 989 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.6% C: 3.0% G: 90.9% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 988.3 0 2 4 8 247.1 0 8 5 3 61.8 0 3 6 3 15.4 0 3 9 263 0.2 0 5 258 10 393 0.1 1 10 383 11 283 0.0 1 0 283 12 1 0.0 1 0 1 150 3 0.0 1 0 3 189 1 0.0 1 0 1 193 2 0.0 1 2 208 12 0.0 1 6 6 223 7 0.0 1 7 224 2 0.0 1 2 226 1 0.0 1 1 228 1 0.0 1 1 237 1 0.0 1 1 257 1 0.0 1 1 258 1 0.0 1 1 262 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.