This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 99.38 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 167,892 (5.3%) Reads that were too short: 24,085 (0.8%) Reads written (passing filters): 143,807 (4.5%) Total basepairs processed: 961,105,341 bp Total written (filtered): 38,362,063 bp (4.0%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 167892 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 667 779.6 0 667 7 6226 779.6 0 6226 8 109023 779.6 0 109023 9 187 779.6 0 187 10 25 779.6 0 25 11 102 779.6 0 102 12 19 779.6 0 19 13 72 779.6 0 72 14 67 779.6 0 67 15 84 779.6 0 84 16 45 779.6 0 45 17 2 779.6 0 2 18 1 779.6 0 1 19 3 779.6 0 3 20 1 779.6 0 1 21 2 779.6 0 2 24 9 779.6 0 9 25 821 779.6 0 821 26 62 779.6 0 62 27 8 779.6 0 8 28 3 779.6 0 3 29 3 779.6 0 3 30 1 779.6 0 1 31 4 779.6 0 4 32 4 779.6 0 4 33 6 779.6 0 6 34 15 779.6 0 15 35 3 779.6 0 3 36 2 779.6 0 2 38 1 779.6 0 1 39 5 779.6 0 5 40 3 779.6 0 3 41 6 779.6 0 6 42 4 779.6 0 4 43 14 779.6 0 14 44 2 779.6 0 2 45 2 779.6 0 2 46 4 779.6 0 4 47 6 779.6 0 6 48 2 779.6 0 2 49 2 779.6 0 2 50 2 779.6 0 2 51 2 779.6 0 2 52 2 779.6 0 2 53 2 779.6 0 2 54 4 779.6 0 4 55 7 779.6 0 7 56 5 779.6 0 5 57 1 779.6 0 1 58 23 779.6 0 23 59 23 779.6 0 23 60 9 779.6 0 9 61 8 779.6 0 8 62 3 779.6 0 3 63 2 779.6 0 2 64 8 779.6 0 8 65 5 779.6 0 5 66 2 779.6 0 2 67 4 779.6 0 4 68 6 779.6 0 6 69 5 779.6 0 5 70 7 779.6 0 7 71 5 779.6 0 5 72 6 779.6 0 6 73 5 779.6 0 5 74 4 779.6 0 4 75 5 779.6 0 5 76 8 779.6 0 8 77 2 779.6 0 2 78 9 779.6 0 9 79 20 779.6 0 20 80 79 779.6 0 79 81 91 779.6 0 91 82 799 779.6 0 799 83 252 779.6 0 252 84 749 779.6 0 749 85 135 779.6 0 135 86 35 779.6 0 35 87 18 779.6 0 18 88 279 779.6 0 279 89 673 779.6 0 673 90 54 779.6 0 54 91 14 779.6 0 14 92 157 779.6 0 157 93 62 779.6 0 62 94 43 779.6 0 43 95 40 779.6 0 40 96 65 779.6 0 65 97 28 779.6 0 28 98 137 779.6 0 137 99 233 779.6 0 233 100 56 779.6 0 56 101 20 779.6 0 20 102 243 779.6 0 243 103 71 779.6 0 71 104 134 779.6 0 134 105 292 779.6 0 292 106 122 779.6 0 122 107 31 779.6 0 31 108 49 779.6 0 49 109 96 779.6 0 96 110 247 779.6 0 247 111 306 779.6 0 306 112 373 779.6 0 373 113 92 779.6 0 92 114 112 779.6 0 112 115 69 779.6 0 69 116 54 779.6 0 54 117 49 779.6 0 49 118 164 779.6 0 164 119 82 779.6 0 82 120 194 779.6 0 194 121 157 779.6 0 157 122 127 779.6 0 127 123 185 779.6 0 185 124 161 779.6 0 161 125 82 779.6 0 82 126 93 779.6 0 93 127 74 779.6 0 74 128 65 779.6 0 65 129 112 779.6 0 112 130 130 779.6 0 130 131 107 779.6 0 107 132 107 779.6 0 107 133 164 779.6 0 164 134 119 779.6 0 119 135 112 779.6 0 112 136 94 779.6 0 94 137 111 779.6 0 111 138 246 779.6 0 246 139 347 779.6 0 347 140 422 779.6 0 422 141 174 779.6 0 174 142 102 779.6 0 102 143 95 779.6 0 95 144 230 779.6 0 230 145 151 779.6 0 151 146 95 779.6 0 95 147 131 779.6 0 131 148 109 779.6 0 109 149 115 779.6 0 115 150 62 779.6 0 62 151 69 779.6 0 69 152 129 779.6 0 129 153 149 779.6 0 149 154 100 779.6 0 100 155 112 779.6 0 112 156 122 779.6 0 122 157 142 779.6 0 142 158 142 779.6 0 142 159 214 779.6 0 214 160 642 779.6 0 642 161 295 779.6 0 295 162 399 779.6 0 399 163 156 779.6 0 156 164 175 779.6 0 175 165 114 779.6 0 114 166 75 779.6 0 75 167 103 779.6 0 103 168 187 779.6 0 187 169 233 779.6 0 233 170 88 779.6 0 88 171 85 779.6 0 85 172 221 779.6 0 221 173 240 779.6 0 240 174 58 779.6 0 58 175 117 779.6 0 117 176 72 779.6 0 72 177 115 779.6 0 115 178 165 779.6 0 165 179 188 779.6 0 188 180 164 779.6 0 164 181 181 779.6 0 181 182 245 779.6 0 245 183 207 779.6 0 207 184 246 779.6 0 246 185 254 779.6 0 254 186 284 779.6 0 284 187 2074 779.6 0 2074 188 1025 779.6 0 1025 189 3332 779.6 0 3332 190 285 779.6 0 285 191 197 779.6 0 197 192 81 779.6 0 81 193 115 779.6 0 115 194 79 779.6 0 79 195 74 779.6 0 74 196 205 779.6 0 205 197 308 779.6 0 308 198 137 779.6 0 137 199 119 779.6 0 119 200 202 779.6 0 202 201 296 779.6 0 296 202 346 779.6 0 346 203 281 779.6 0 281 204 168 779.6 0 168 205 180 779.6 0 180 206 244 779.6 0 244 207 240 779.6 0 240 208 213 779.6 0 213 209 238 779.6 0 238 210 234 779.6 0 234 211 192 779.6 0 192 212 165 779.6 0 165 213 174 779.6 0 174 214 155 779.6 0 155 215 152 779.6 0 152 216 207 779.6 0 207 217 191 779.6 0 191 218 199 779.6 0 199 219 195 779.6 0 195 220 150 779.6 0 150 221 248 779.6 0 248 222 358 779.6 0 358 223 237 779.6 0 237 224 120 779.6 0 120 225 205 779.6 0 205 226 178 779.6 0 178 227 179 779.6 0 179 228 185 779.6 0 185 229 226 779.6 0 226 230 283 779.6 0 283 231 346 779.6 0 346 232 264 779.6 0 264 233 299 779.6 0 299 234 250 779.6 0 250 235 107 779.6 0 107 236 121 779.6 0 121 237 190 779.6 0 190 238 170 779.6 0 170 239 447 779.6 0 447 240 367 779.6 0 367 241 320 779.6 0 320 242 233 779.6 0 233 243 221 779.6 0 221 244 199 779.6 0 199 245 163 779.6 0 163 246 198 779.6 0 198 247 95 779.6 0 95 248 70 779.6 0 70 249 84 779.6 0 84 250 198 779.6 0 198 251 243 779.6 0 243 252 155 779.6 0 155 253 135 779.6 0 135 254 205 779.6 0 205 255 222 779.6 0 222 256 189 779.6 0 189 257 118 779.6 0 118 258 130 779.6 0 130 259 117 779.6 0 117 260 145 779.6 0 145 261 173 779.6 0 173 262 175 779.6 0 175 263 156 779.6 0 156 264 201 779.6 0 201 265 215 779.6 0 215 266 240 779.6 0 240 267 118 779.6 0 118 268 113 779.6 0 113 269 139 779.6 0 139 270 252 779.6 0 252 271 203 779.6 0 203 272 225 779.6 0 225 273 213 779.6 0 213 274 235 779.6 0 235 275 301 779.6 0 301 276 329 779.6 0 329 277 1050 779.6 0 1050 278 333 779.6 0 333 279 291 779.6 0 291 280 212 779.6 0 212 281 147 779.6 0 147 282 283 779.6 0 283 283 376 779.6 0 376 284 242 779.6 0 242 285 236 779.6 0 236 286 179 779.6 0 179 287 212 779.6 0 212 288 203 779.6 0 203 289 279 779.6 0 279 290 191 779.6 0 191 291 255 779.6 0 255 292 312 779.6 0 312 293 186 779.6 0 186 294 180 779.6 0 180 295 309 779.6 0 309 296 300 779.6 0 300 297 146 779.6 0 146 298 199 779.6 0 199 299 332 779.6 0 332 300 514 779.6 0 514 301 1686 779.6 0 1686 Finished in 6.56 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 143,807 Reads with adapters: 115,959 (80.6%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 115,950 (80.6%) Total basepairs processed: 38,362,063 bp Total written (filtered): 31,660,841 bp (82.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 115959 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 40 0.0 3 0 0 39 1 19 964 0.0 3 0 933 25 4 2 20 114524 0.0 4 112821 1640 59 3 1 21 334 0.0 4 90 239 4 1 22 27 0.0 4 1 2 19 0 5 23 46 0.0 4 1 0 1 40 4 24 2 0.0 4 0 0 0 1 1 25 1 0.0 4 0 1 28 1 0.0 4 1 38 2 0.0 4 0 0 0 0 2 47 1 0.0 4 1 72 5 0.0 4 0 0 0 0 5 88 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 184 6 0.0 4 0 0 0 0 6 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.79 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 115,950 Reads with adapters: 2,972 (2.6%) Reads written (passing filters): 115,950 (100.0%) Total basepairs processed: 31,660,841 bp Total written (filtered): 31,624,870 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2972 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 0.8% G: 97.3% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 1811.7 0 4 4 2 452.9 0 2 5 3 113.2 0 3 6 1 28.3 0 1 10 166 0.1 1 6 160 11 2776 0.0 1 7 2769 14 2 0.0 1 1 1 93 1 0.0 1 0 1 151 1 0.0 1 0 1 209 14 0.0 1 1 13 268 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.