This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.62 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 64,770 (2.0%) Reads that were too short: 6,370 (0.2%) Reads written (passing filters): 58,400 (1.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 13,285,950 bp (1.4%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 64770 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6529 779.6 0 6529 7 14204 779.6 0 14204 8 11067 779.6 0 11067 9 34 779.6 0 34 10 21 779.6 0 21 11 12 779.6 0 12 12 14 779.6 0 14 13 17 779.6 0 17 14 11 779.6 0 11 15 11 779.6 0 11 16 54 779.6 0 54 17 18 779.6 0 18 18 16 779.6 0 16 19 62 779.6 0 62 20 134 779.6 0 134 21 207 779.6 0 207 22 22 779.6 0 22 23 18 779.6 0 18 24 8 779.6 0 8 25 10 779.6 0 10 26 15 779.6 0 15 27 9 779.6 0 9 28 9 779.6 0 9 29 18 779.6 0 18 30 14 779.6 0 14 31 12 779.6 0 12 32 14 779.6 0 14 33 22 779.6 0 22 34 25 779.6 0 25 35 17 779.6 0 17 36 12 779.6 0 12 37 15 779.6 0 15 38 139 779.6 0 139 39 55 779.6 0 55 40 41 779.6 0 41 41 206 779.6 0 206 42 14 779.6 0 14 43 14 779.6 0 14 44 21 779.6 0 21 45 13 779.6 0 13 46 8 779.6 0 8 47 17 779.6 0 17 48 17 779.6 0 17 49 21 779.6 0 21 50 15 779.6 0 15 51 7 779.6 0 7 52 10 779.6 0 10 53 14 779.6 0 14 54 12 779.6 0 12 55 10 779.6 0 10 56 15 779.6 0 15 57 15 779.6 0 15 58 9 779.6 0 9 59 16 779.6 0 16 60 10 779.6 0 10 61 11 779.6 0 11 62 12 779.6 0 12 63 9 779.6 0 9 64 11 779.6 0 11 65 6 779.6 0 6 66 12 779.6 0 12 67 12 779.6 0 12 68 15 779.6 0 15 69 15 779.6 0 15 70 10 779.6 0 10 71 14 779.6 0 14 72 17 779.6 0 17 73 19 779.6 0 19 74 21 779.6 0 21 75 18 779.6 0 18 76 70 779.6 0 70 77 108 779.6 0 108 78 119 779.6 0 119 79 16 779.6 0 16 80 46 779.6 0 46 81 12 779.6 0 12 82 13 779.6 0 13 83 13 779.6 0 13 84 22 779.6 0 22 85 21 779.6 0 21 86 8 779.6 0 8 87 16 779.6 0 16 88 15 779.6 0 15 89 11 779.6 0 11 90 11 779.6 0 11 91 25 779.6 0 25 92 106 779.6 0 106 93 165 779.6 0 165 94 69 779.6 0 69 95 53 779.6 0 53 96 103 779.6 0 103 97 273 779.6 0 273 98 201 779.6 0 201 99 12 779.6 0 12 100 5 779.6 0 5 101 17 779.6 0 17 102 18 779.6 0 18 103 6 779.6 0 6 104 17 779.6 0 17 105 14 779.6 0 14 106 11 779.6 0 11 107 18 779.6 0 18 108 11 779.6 0 11 109 7 779.6 0 7 110 12 779.6 0 12 111 16 779.6 0 16 112 18 779.6 0 18 113 16 779.6 0 16 114 11 779.6 0 11 115 20 779.6 0 20 116 23 779.6 0 23 117 34 779.6 0 34 118 6 779.6 0 6 119 15 779.6 0 15 120 19 779.6 0 19 121 10 779.6 0 10 122 17 779.6 0 17 123 11 779.6 0 11 124 10 779.6 0 10 125 15 779.6 0 15 126 8 779.6 0 8 127 17 779.6 0 17 128 36 779.6 0 36 129 44 779.6 0 44 130 177 779.6 0 177 131 175 779.6 0 175 132 713 779.6 0 713 133 87 779.6 0 87 134 568 779.6 0 568 135 61 779.6 0 61 136 301 779.6 0 301 137 37 779.6 0 37 138 33 779.6 0 33 139 8 779.6 0 8 140 16 779.6 0 16 141 17 779.6 0 17 142 39 779.6 0 39 143 39 779.6 0 39 144 23 779.6 0 23 145 24 779.6 0 24 146 14 779.6 0 14 147 13 779.6 0 13 148 10 779.6 0 10 149 11 779.6 0 11 150 11 779.6 0 11 151 30 779.6 0 30 152 45 779.6 0 45 153 9 779.6 0 9 154 24 779.6 0 24 155 18 779.6 0 18 156 22 779.6 0 22 157 56 779.6 0 56 158 37 779.6 0 37 159 26 779.6 0 26 160 28 779.6 0 28 161 20 779.6 0 20 162 40 779.6 0 40 163 57 779.6 0 57 164 73 779.6 0 73 165 126 779.6 0 126 166 170 779.6 0 170 167 273 779.6 0 273 168 286 779.6 0 286 169 3141 779.6 0 3141 170 2565 779.6 0 2565 171 12860 779.6 0 12860 172 78 779.6 0 78 173 19 779.6 0 19 174 16 779.6 0 16 175 7 779.6 0 7 176 14 779.6 0 14 177 11 779.6 0 11 178 53 779.6 0 53 179 14 779.6 0 14 180 16 779.6 0 16 181 13 779.6 0 13 182 12 779.6 0 12 183 19 779.6 0 19 184 23 779.6 0 23 185 19 779.6 0 19 186 14 779.6 0 14 187 5 779.6 0 5 188 11 779.6 0 11 189 8 779.6 0 8 190 11 779.6 0 11 191 15 779.6 0 15 192 79 779.6 0 79 193 16 779.6 0 16 194 33 779.6 0 33 195 10 779.6 0 10 196 6 779.6 0 6 197 6 779.6 0 6 198 18 779.6 0 18 199 8 779.6 0 8 200 14 779.6 0 14 201 7 779.6 0 7 202 19 779.6 0 19 203 33 779.6 0 33 204 71 779.6 0 71 205 37 779.6 0 37 206 15 779.6 0 15 207 9 779.6 0 9 208 13 779.6 0 13 209 7 779.6 0 7 210 17 779.6 0 17 211 23 779.6 0 23 212 29 779.6 0 29 213 16 779.6 0 16 214 14 779.6 0 14 215 24 779.6 0 24 216 26 779.6 0 26 217 31 779.6 0 31 218 22 779.6 0 22 219 48 779.6 0 48 220 34 779.6 0 34 221 211 779.6 0 211 222 191 779.6 0 191 223 506 779.6 0 506 224 1123 779.6 0 1123 225 22 779.6 0 22 226 17 779.6 0 17 227 17 779.6 0 17 228 10 779.6 0 10 229 14 779.6 0 14 230 33 779.6 0 33 231 28 779.6 0 28 232 30 779.6 0 30 233 21 779.6 0 21 234 12 779.6 0 12 235 22 779.6 0 22 236 64 779.6 0 64 237 89 779.6 0 89 238 83 779.6 0 83 239 28 779.6 0 28 240 21 779.6 0 21 241 11 779.6 0 11 242 15 779.6 0 15 243 9 779.6 0 9 244 9 779.6 0 9 245 19 779.6 0 19 246 9 779.6 0 9 247 5 779.6 0 5 248 5 779.6 0 5 249 10 779.6 0 10 250 12 779.6 0 12 251 7 779.6 0 7 252 11 779.6 0 11 253 16 779.6 0 16 254 16 779.6 0 16 255 43 779.6 0 43 256 22 779.6 0 22 257 138 779.6 0 138 258 36 779.6 0 36 259 7 779.6 0 7 260 7 779.6 0 7 261 38 779.6 0 38 262 16 779.6 0 16 263 12 779.6 0 12 264 24 779.6 0 24 265 24 779.6 0 24 266 12 779.6 0 12 267 11 779.6 0 11 268 40 779.6 0 40 269 17 779.6 0 17 270 20 779.6 0 20 271 222 779.6 0 222 272 290 779.6 0 290 273 146 779.6 0 146 274 99 779.6 0 99 275 13 779.6 0 13 276 4 779.6 0 4 277 12 779.6 0 12 278 26 779.6 0 26 279 14 779.6 0 14 280 14 779.6 0 14 281 11 779.6 0 11 282 10 779.6 0 10 283 9 779.6 0 9 284 10 779.6 0 10 285 36 779.6 0 36 286 30 779.6 0 30 287 9 779.6 0 9 288 43 779.6 0 43 289 37 779.6 0 37 290 41 779.6 0 41 291 12 779.6 0 12 292 49 779.6 0 49 293 11 779.6 0 11 294 27 779.6 0 27 295 26 779.6 0 26 296 156 779.6 0 156 297 949 779.6 0 949 298 305 779.6 0 305 299 14 779.6 0 14 300 20 779.6 0 20 301 74 779.6 0 74 Finished in 2.63 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 58,400 Reads with adapters: 18,106 (31.0%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 18,090 (31.0%) Total basepairs processed: 13,285,950 bp Total written (filtered): 5,000,316 bp (37.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18106 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 4 16 129 0.0 3 0 116 10 3 17 13899 0.0 3 13451 377 53 18 18 44 0.0 3 5 32 2 5 19 59 0.0 3 4 43 9 3 20 3914 0.0 3 3720 136 15 43 21 10 0.0 3 3 6 0 1 22 4 0.0 3 1 0 2 1 23 5 0.0 3 0 0 0 5 27 2 0.0 3 1 0 1 50 2 0.0 3 0 0 0 2 53 7 0.0 3 0 0 0 7 57 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 1 187 1 0.0 3 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 18,090 Reads with adapters: 1,314 (7.3%) Reads written (passing filters): 18,090 (100.0%) Total basepairs processed: 5,000,316 bp Total written (filtered): 4,996,282 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1314 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.9% C: 46.5% G: 21.0% T: 11.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1225 282.7 0 1225 4 87 70.7 0 87 5 1 17.7 0 1 6 1 4.4 0 1