This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 98.58 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 87,004 (2.7%) Reads that were too short: 18,278 (0.6%) Reads written (passing filters): 68,726 (2.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 14,915,036 bp (1.6%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 87004 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1260 779.6 0 1260 7 12456 779.6 0 12456 8 3419 779.6 0 3419 9 26 779.6 0 26 10 7 779.6 0 7 11 19 779.6 0 19 12 13 779.6 0 13 13 10 779.6 0 10 14 17 779.6 0 17 15 7 779.6 0 7 16 12 779.6 0 12 17 10 779.6 0 10 18 7 779.6 0 7 19 17 779.6 0 17 20 18 779.6 0 18 21 17 779.6 0 17 22 17 779.6 0 17 23 14 779.6 0 14 24 12 779.6 0 12 25 24 779.6 0 24 26 17 779.6 0 17 27 13 779.6 0 13 28 22 779.6 0 22 29 24 779.6 0 24 30 21 779.6 0 21 31 48 779.6 0 48 32 437 779.6 0 437 33 15 779.6 0 15 34 17098 779.6 0 17098 35 38 779.6 0 38 36 16 779.6 0 16 37 18 779.6 0 18 38 120 779.6 0 120 39 34 779.6 0 34 40 53 779.6 0 53 41 20 779.6 0 20 42 28 779.6 0 28 43 55 779.6 0 55 44 32 779.6 0 32 45 45 779.6 0 45 46 12 779.6 0 12 47 12 779.6 0 12 48 4 779.6 0 4 49 8 779.6 0 8 50 13 779.6 0 13 51 14 779.6 0 14 52 12 779.6 0 12 53 101 779.6 0 101 54 40 779.6 0 40 55 78 779.6 0 78 56 16 779.6 0 16 57 26 779.6 0 26 58 11 779.6 0 11 59 13 779.6 0 13 60 12 779.6 0 12 61 22 779.6 0 22 62 8 779.6 0 8 63 14 779.6 0 14 64 7 779.6 0 7 65 20 779.6 0 20 66 41 779.6 0 41 67 23 779.6 0 23 68 31 779.6 0 31 69 40 779.6 0 40 70 28 779.6 0 28 71 33 779.6 0 33 72 37 779.6 0 37 73 21 779.6 0 21 74 24 779.6 0 24 75 7 779.6 0 7 76 14 779.6 0 14 77 23 779.6 0 23 78 15 779.6 0 15 79 22 779.6 0 22 80 25 779.6 0 25 81 30 779.6 0 30 82 34 779.6 0 34 83 38 779.6 0 38 84 24 779.6 0 24 85 15 779.6 0 15 86 29 779.6 0 29 87 22 779.6 0 22 88 32 779.6 0 32 89 22 779.6 0 22 90 10 779.6 0 10 91 58 779.6 0 58 92 64 779.6 0 64 93 87 779.6 0 87 94 29 779.6 0 29 95 23 779.6 0 23 96 21 779.6 0 21 97 19 779.6 0 19 98 34 779.6 0 34 99 39 779.6 0 39 100 14 779.6 0 14 101 34 779.6 0 34 102 59 779.6 0 59 103 129 779.6 0 129 104 169 779.6 0 169 105 240 779.6 0 240 106 15 779.6 0 15 107 37 779.6 0 37 108 119 779.6 0 119 109 102 779.6 0 102 110 27 779.6 0 27 111 117 779.6 0 117 112 86 779.6 0 86 113 225 779.6 0 225 114 77 779.6 0 77 115 55 779.6 0 55 116 29 779.6 0 29 117 44 779.6 0 44 118 26 779.6 0 26 119 38 779.6 0 38 120 67 779.6 0 67 121 63 779.6 0 63 122 57 779.6 0 57 123 120 779.6 0 120 124 194 779.6 0 194 125 1309 779.6 0 1309 126 610 779.6 0 610 127 1036 779.6 0 1036 128 57 779.6 0 57 129 71 779.6 0 71 130 231 779.6 0 231 131 51 779.6 0 51 132 77 779.6 0 77 133 102 779.6 0 102 134 87 779.6 0 87 135 107 779.6 0 107 136 126 779.6 0 126 137 74 779.6 0 74 138 117 779.6 0 117 139 166 779.6 0 166 140 84 779.6 0 84 141 68 779.6 0 68 142 45 779.6 0 45 143 151 779.6 0 151 144 183 779.6 0 183 145 142 779.6 0 142 146 145 779.6 0 145 147 120 779.6 0 120 148 57 779.6 0 57 149 56 779.6 0 56 150 377 779.6 0 377 151 302 779.6 0 302 152 810 779.6 0 810 153 82 779.6 0 82 154 172 779.6 0 172 155 138 779.6 0 138 156 383 779.6 0 383 157 240 779.6 0 240 158 337 779.6 0 337 159 565 779.6 0 565 160 5212 779.6 0 5212 161 4053 779.6 0 4053 162 6865 779.6 0 6865 163 189 779.6 0 189 164 271 779.6 0 271 165 143 779.6 0 143 166 192 779.6 0 192 167 81 779.6 0 81 168 104 779.6 0 104 169 81 779.6 0 81 170 82 779.6 0 82 171 94 779.6 0 94 172 138 779.6 0 138 173 47 779.6 0 47 174 37 779.6 0 37 175 60 779.6 0 60 176 63 779.6 0 63 177 75 779.6 0 75 178 97 779.6 0 97 179 102 779.6 0 102 180 191 779.6 0 191 181 402 779.6 0 402 182 674 779.6 0 674 183 215 779.6 0 215 184 110 779.6 0 110 185 105 779.6 0 105 186 129 779.6 0 129 187 70 779.6 0 70 188 106 779.6 0 106 189 160 779.6 0 160 190 118 779.6 0 118 191 97 779.6 0 97 192 54 779.6 0 54 193 39 779.6 0 39 194 24 779.6 0 24 195 36 779.6 0 36 196 74 779.6 0 74 197 82 779.6 0 82 198 42 779.6 0 42 199 40 779.6 0 40 200 41 779.6 0 41 201 117 779.6 0 117 202 96 779.6 0 96 203 51 779.6 0 51 204 30 779.6 0 30 205 65 779.6 0 65 206 54 779.6 0 54 207 72 779.6 0 72 208 130 779.6 0 130 209 113 779.6 0 113 210 101 779.6 0 101 211 150 779.6 0 150 212 959 779.6 0 959 213 992 779.6 0 992 214 864 779.6 0 864 215 150 779.6 0 150 216 198 779.6 0 198 217 221 779.6 0 221 218 229 779.6 0 229 219 74 779.6 0 74 220 66 779.6 0 66 221 137 779.6 0 137 222 189 779.6 0 189 223 78 779.6 0 78 224 94 779.6 0 94 225 270 779.6 0 270 226 228 779.6 0 228 227 138 779.6 0 138 228 154 779.6 0 154 229 152 779.6 0 152 230 71 779.6 0 71 231 48 779.6 0 48 232 69 779.6 0 69 233 97 779.6 0 97 234 59 779.6 0 59 235 59 779.6 0 59 236 91 779.6 0 91 237 162 779.6 0 162 238 108 779.6 0 108 239 243 779.6 0 243 240 265 779.6 0 265 241 225 779.6 0 225 242 116 779.6 0 116 243 144 779.6 0 144 244 106 779.6 0 106 245 230 779.6 0 230 246 204 779.6 0 204 247 104 779.6 0 104 248 68 779.6 0 68 249 127 779.6 0 127 250 140 779.6 0 140 251 126 779.6 0 126 252 105 779.6 0 105 253 115 779.6 0 115 254 245 779.6 0 245 255 447 779.6 0 447 256 404 779.6 0 404 257 244 779.6 0 244 258 152 779.6 0 152 259 114 779.6 0 114 260 204 779.6 0 204 261 134 779.6 0 134 262 132 779.6 0 132 263 101 779.6 0 101 264 142 779.6 0 142 265 227 779.6 0 227 266 585 779.6 0 585 267 333 779.6 0 333 268 306 779.6 0 306 269 134 779.6 0 134 270 236 779.6 0 236 271 589 779.6 0 589 272 208 779.6 0 208 273 221 779.6 0 221 274 304 779.6 0 304 275 347 779.6 0 347 276 189 779.6 0 189 277 230 779.6 0 230 278 228 779.6 0 228 279 145 779.6 0 145 280 160 779.6 0 160 281 151 779.6 0 151 282 483 779.6 0 483 283 211 779.6 0 211 284 158 779.6 0 158 285 130 779.6 0 130 286 104 779.6 0 104 287 201 779.6 0 201 288 108 779.6 0 108 289 162 779.6 0 162 290 76 779.6 0 76 291 72 779.6 0 72 292 63 779.6 0 63 293 41 779.6 0 41 294 20 779.6 0 20 295 42 779.6 0 42 296 22 779.6 0 22 297 34 779.6 0 34 298 81 779.6 0 81 299 93 779.6 0 93 300 78 779.6 0 78 301 50 779.6 0 50 Finished in 3.25 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 68,726 Reads with adapters: 13,674 (19.9%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 13,666 (19.9%) Total basepairs processed: 14,915,036 bp Total written (filtered): 3,738,242 bp (25.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13674 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 14 0.0 3 0 0 3 0 11 19 223 0.0 3 0 216 7 20 12760 0.0 4 12531 224 4 1 21 603 0.0 4 577 24 2 22 6 0.0 4 0 2 2 1 1 23 6 0.0 4 0 0 0 6 25 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 2 127 3 0.0 4 0 0 0 0 3 128 45 0.0 4 0 0 0 0 45 166 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 13,666 Reads with adapters: 315 (2.3%) Reads written (passing filters): 13,666 (100.0%) Total basepairs processed: 3,738,242 bp Total written (filtered): 3,661,544 bp (97.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 315 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.3% C: 0.6% G: 8.9% T: 89.8% none/other: 0.3% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "T" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 213.5 0 1 5 1 13.3 0 1 10 1 0.0 1 0 1 11 24 0.0 1 0 24 12 2 0.0 1 0 2 13 1 0.0 1 0 1 15 1 0.0 1 0 1 152 1 0.0 1 0 1 268 1 0.0 1 1 269 198 0.0 1 191 7 270 82 0.0 1 78 4 271 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.