This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.75 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 351,199 (11.0%) Reads that were too short: 27,953 (0.9%) Reads written (passing filters): 323,246 (10.1%) Total basepairs processed: 961,105,341 bp Total written (filtered): 49,780,497 bp (5.2%) === Adapter 1 === Sequence: CACTCT; Type: regular 5'; Length: 6; Trimmed: 351199 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 288 779.6 0 288 7 4086 779.6 0 4086 8 191 779.6 0 191 9 14622 779.6 0 14622 10 20 779.6 0 20 11 12 779.6 0 12 12 23 779.6 0 23 13 9 779.6 0 9 14 20 779.6 0 20 15 15 779.6 0 15 16 25 779.6 0 25 17 18 779.6 0 18 18 9 779.6 0 9 19 21 779.6 0 21 20 44 779.6 0 44 21 37 779.6 0 37 22 34 779.6 0 34 23 7 779.6 0 7 24 16 779.6 0 16 25 11 779.6 0 11 26 19 779.6 0 19 27 16 779.6 0 16 28 14 779.6 0 14 29 14 779.6 0 14 30 20 779.6 0 20 31 33 779.6 0 33 32 37 779.6 0 37 33 23 779.6 0 23 34 40 779.6 0 40 35 67 779.6 0 67 36 22 779.6 0 22 37 13 779.6 0 13 38 17 779.6 0 17 39 16 779.6 0 16 40 13 779.6 0 13 41 35 779.6 0 35 42 27 779.6 0 27 43 165 779.6 0 165 44 54 779.6 0 54 45 36 779.6 0 36 46 550 779.6 0 550 47 114 779.6 0 114 48 234 779.6 0 234 49 99 779.6 0 99 50 121 779.6 0 121 51 24 779.6 0 24 52 16 779.6 0 16 53 37 779.6 0 37 54 96 779.6 0 96 55 41 779.6 0 41 56 56 779.6 0 56 57 12 779.6 0 12 58 9 779.6 0 9 59 25 779.6 0 25 60 8 779.6 0 8 61 40 779.6 0 40 62 16 779.6 0 16 63 17 779.6 0 17 64 16 779.6 0 16 65 7 779.6 0 7 66 12 779.6 0 12 67 54 779.6 0 54 68 14 779.6 0 14 69 16 779.6 0 16 70 27 779.6 0 27 71 25 779.6 0 25 72 14 779.6 0 14 73 27 779.6 0 27 74 11 779.6 0 11 75 18 779.6 0 18 76 19 779.6 0 19 77 32 779.6 0 32 78 23 779.6 0 23 79 58 779.6 0 58 80 51 779.6 0 51 81 43 779.6 0 43 82 61 779.6 0 61 83 32 779.6 0 32 84 53 779.6 0 53 85 24 779.6 0 24 86 34 779.6 0 34 87 44 779.6 0 44 88 74 779.6 0 74 89 152 779.6 0 152 90 119 779.6 0 119 91 61 779.6 0 61 92 317 779.6 0 317 93 67 779.6 0 67 94 199 779.6 0 199 95 137 779.6 0 137 96 114 779.6 0 114 97 112 779.6 0 112 98 66 779.6 0 66 99 57 779.6 0 57 100 55 779.6 0 55 101 24 779.6 0 24 102 161 779.6 0 161 103 109 779.6 0 109 104 446 779.6 0 446 105 1048 779.6 0 1048 106 1651 779.6 0 1651 107 74 779.6 0 74 108 135 779.6 0 135 109 117 779.6 0 117 110 204 779.6 0 204 111 72 779.6 0 72 112 368 779.6 0 368 113 185 779.6 0 185 114 191 779.6 0 191 115 65 779.6 0 65 116 50 779.6 0 50 117 49 779.6 0 49 118 61 779.6 0 61 119 172 779.6 0 172 120 107 779.6 0 107 121 59 779.6 0 59 122 138 779.6 0 138 123 59 779.6 0 59 124 186 779.6 0 186 125 154 779.6 0 154 126 156 779.6 0 156 127 406 779.6 0 406 128 350 779.6 0 350 129 510 779.6 0 510 130 148 779.6 0 148 131 224 779.6 0 224 132 101 779.6 0 101 133 206 779.6 0 206 134 729 779.6 0 729 135 338 779.6 0 338 136 285 779.6 0 285 137 126 779.6 0 126 138 161 779.6 0 161 139 345 779.6 0 345 140 374 779.6 0 374 141 373 779.6 0 373 142 370 779.6 0 370 143 646 779.6 0 646 144 1592 779.6 0 1592 145 1435 779.6 0 1435 146 22608 779.6 0 22608 147 11154 779.6 0 11154 148 29553 779.6 0 29553 149 499 779.6 0 499 150 252 779.6 0 252 151 891 779.6 0 891 152 1001 779.6 0 1001 153 2142 779.6 0 2142 154 485 779.6 0 485 155 567 779.6 0 567 156 873 779.6 0 873 157 1242 779.6 0 1242 158 1380 779.6 0 1380 159 3167 779.6 0 3167 160 4369 779.6 0 4369 161 74504 779.6 0 74504 162 53112 779.6 0 53112 163 69975 779.6 0 69975 164 543 779.6 0 543 165 361 779.6 0 361 166 243 779.6 0 243 167 260 779.6 0 260 168 145 779.6 0 145 169 81 779.6 0 81 170 67 779.6 0 67 171 71 779.6 0 71 172 97 779.6 0 97 173 188 779.6 0 188 174 171 779.6 0 171 175 117 779.6 0 117 176 114 779.6 0 114 177 146 779.6 0 146 178 91 779.6 0 91 179 184 779.6 0 184 180 166 779.6 0 166 181 302 779.6 0 302 182 555 779.6 0 555 183 582 779.6 0 582 184 281 779.6 0 281 185 138 779.6 0 138 186 104 779.6 0 104 187 221 779.6 0 221 188 77 779.6 0 77 189 140 779.6 0 140 190 165 779.6 0 165 191 162 779.6 0 162 192 217 779.6 0 217 193 86 779.6 0 86 194 71 779.6 0 71 195 51 779.6 0 51 196 81 779.6 0 81 197 131 779.6 0 131 198 121 779.6 0 121 199 58 779.6 0 58 200 85 779.6 0 85 201 90 779.6 0 90 202 255 779.6 0 255 203 215 779.6 0 215 204 158 779.6 0 158 205 98 779.6 0 98 206 136 779.6 0 136 207 86 779.6 0 86 208 153 779.6 0 153 209 159 779.6 0 159 210 130 779.6 0 130 211 103 779.6 0 103 212 122 779.6 0 122 213 140 779.6 0 140 214 182 779.6 0 182 215 175 779.6 0 175 216 196 779.6 0 196 217 255 779.6 0 255 218 332 779.6 0 332 219 264 779.6 0 264 220 102 779.6 0 102 221 113 779.6 0 113 222 257 779.6 0 257 223 272 779.6 0 272 224 171 779.6 0 171 225 164 779.6 0 164 226 275 779.6 0 275 227 166 779.6 0 166 228 200 779.6 0 200 229 127 779.6 0 127 230 86 779.6 0 86 231 80 779.6 0 80 232 89 779.6 0 89 233 134 779.6 0 134 234 155 779.6 0 155 235 97 779.6 0 97 236 86 779.6 0 86 237 98 779.6 0 98 238 192 779.6 0 192 239 233 779.6 0 233 240 1399 779.6 0 1399 241 1743 779.6 0 1743 242 3955 779.6 0 3955 243 894 779.6 0 894 244 430 779.6 0 430 245 202 779.6 0 202 246 136 779.6 0 136 247 94 779.6 0 94 248 118 779.6 0 118 249 102 779.6 0 102 250 163 779.6 0 163 251 194 779.6 0 194 252 280 779.6 0 280 253 254 779.6 0 254 254 338 779.6 0 338 255 254 779.6 0 254 256 279 779.6 0 279 257 192 779.6 0 192 258 95 779.6 0 95 259 51 779.6 0 51 260 59 779.6 0 59 261 51 779.6 0 51 262 78 779.6 0 78 263 96 779.6 0 96 264 207 779.6 0 207 265 256 779.6 0 256 266 282 779.6 0 282 267 526 779.6 0 526 268 571 779.6 0 571 269 461 779.6 0 461 270 401 779.6 0 401 271 289 779.6 0 289 272 192 779.6 0 192 273 132 779.6 0 132 274 114 779.6 0 114 275 157 779.6 0 157 276 196 779.6 0 196 277 1586 779.6 0 1586 278 951 779.6 0 951 279 866 779.6 0 866 280 188 779.6 0 188 281 136 779.6 0 136 282 130 779.6 0 130 283 194 779.6 0 194 284 88 779.6 0 88 285 204 779.6 0 204 286 103 779.6 0 103 287 176 779.6 0 176 288 120 779.6 0 120 289 122 779.6 0 122 290 106 779.6 0 106 291 160 779.6 0 160 292 168 779.6 0 168 293 173 779.6 0 173 294 118 779.6 0 118 295 90 779.6 0 90 296 108 779.6 0 108 297 165 779.6 0 165 298 102 779.6 0 102 299 193 779.6 0 193 300 74 779.6 0 74 301 135 779.6 0 135 Finished in 12.24 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 323,246 Reads with adapters: 15,288 (4.7%) Reads that were too short: 216 (0.1%) Reads written (passing filters): 15,072 (4.7%) Total basepairs processed: 49,780,497 bp Total written (filtered): 4,127,181 bp (8.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15288 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 6 3 16 224 0.0 3 0 215 6 3 17 14642 0.0 3 14170 419 39 14 18 43 0.0 3 3 34 4 2 19 15 0.0 3 0 0 7 8 20 24 0.0 3 0 1 0 23 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 2 0.0 3 1 0 0 1 40 1 0.0 3 0 0 0 1 50 1 0.0 3 0 1 52 4 0.0 3 0 0 0 4 53 8 0.0 3 0 0 0 8 55 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 67 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 1 69 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 84 2 0.0 3 0 1 0 1 85 1 0.0 3 0 0 0 1 87 42 0.0 3 0 0 0 42 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 12 0.0 3 0 0 0 12 111 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 31 0.0 3 0 0 0 31 128 97 0.0 3 0 0 1 96 129 5 0.0 3 0 0 1 4 131 11 0.0 3 0 0 0 11 132 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 22 0.0 3 0 0 0 22 141 4 0.0 3 0 0 0 4 142 22 0.0 3 0 0 0 22 143 11 0.0 3 0 0 0 11 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 1 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 15,072 Reads with adapters: 770 (5.1%) Reads written (passing filters): 15,072 (100.0%) Total basepairs processed: 4,127,181 bp Total written (filtered): 4,124,754 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 770 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.7% C: 4.2% G: 19.4% T: 20.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 655 235.5 0 655 4 113 58.9 0 113 5 2 14.7 0 2