This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCTCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.53 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 30,556 (1.0%) Reads that were too short: 1,742 (0.1%) Reads written (passing filters): 28,814 (0.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 7,023,251 bp (0.7%) === Adapter 1 === Sequence: TTCTCG; Type: regular 5'; Length: 6; Trimmed: 30556 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 186 779.6 0 186 7 151 779.6 0 151 8 435 779.6 0 435 9 14654 779.6 0 14654 10 48 779.6 0 48 11 11 779.6 0 11 12 13 779.6 0 13 13 12 779.6 0 12 14 13 779.6 0 13 15 10 779.6 0 10 16 4 779.6 0 4 17 14 779.6 0 14 18 11 779.6 0 11 19 18 779.6 0 18 20 21 779.6 0 21 21 8 779.6 0 8 22 5 779.6 0 5 23 6 779.6 0 6 24 11 779.6 0 11 25 15 779.6 0 15 26 10 779.6 0 10 27 13 779.6 0 13 28 6 779.6 0 6 29 4 779.6 0 4 30 10 779.6 0 10 31 13 779.6 0 13 32 11 779.6 0 11 33 13 779.6 0 13 34 25 779.6 0 25 35 9 779.6 0 9 36 6 779.6 0 6 37 11 779.6 0 11 38 12 779.6 0 12 39 6 779.6 0 6 40 5 779.6 0 5 41 6 779.6 0 6 42 38 779.6 0 38 43 26 779.6 0 26 44 152 779.6 0 152 45 70 779.6 0 70 46 7 779.6 0 7 47 18 779.6 0 18 48 12 779.6 0 12 49 9 779.6 0 9 50 8 779.6 0 8 51 13 779.6 0 13 52 43 779.6 0 43 53 14 779.6 0 14 54 112 779.6 0 112 55 9 779.6 0 9 56 13 779.6 0 13 57 20 779.6 0 20 58 9 779.6 0 9 59 23 779.6 0 23 60 18 779.6 0 18 61 3 779.6 0 3 62 30 779.6 0 30 63 12 779.6 0 12 64 11 779.6 0 11 65 14 779.6 0 14 66 11 779.6 0 11 67 7 779.6 0 7 68 15 779.6 0 15 69 7 779.6 0 7 70 19 779.6 0 19 71 15 779.6 0 15 72 17 779.6 0 17 73 17 779.6 0 17 74 15 779.6 0 15 75 36 779.6 0 36 76 90 779.6 0 90 77 24 779.6 0 24 78 5 779.6 0 5 79 13 779.6 0 13 80 8 779.6 0 8 81 12 779.6 0 12 82 11 779.6 0 11 83 25 779.6 0 25 84 21 779.6 0 21 85 32 779.6 0 32 86 18 779.6 0 18 87 28 779.6 0 28 88 31 779.6 0 31 89 36 779.6 0 36 90 576 779.6 0 576 91 416 779.6 0 416 92 415 779.6 0 415 93 22 779.6 0 22 94 68 779.6 0 68 95 64 779.6 0 64 96 122 779.6 0 122 97 121 779.6 0 121 98 145 779.6 0 145 99 33 779.6 0 33 100 37 779.6 0 37 101 63 779.6 0 63 102 65 779.6 0 65 103 40 779.6 0 40 104 79 779.6 0 79 105 137 779.6 0 137 106 182 779.6 0 182 107 69 779.6 0 69 108 37 779.6 0 37 109 57 779.6 0 57 110 132 779.6 0 132 111 3071 779.6 0 3071 112 423 779.6 0 423 113 2472 779.6 0 2472 114 49 779.6 0 49 115 9 779.6 0 9 116 10 779.6 0 10 117 9 779.6 0 9 118 14 779.6 0 14 119 5 779.6 0 5 120 12 779.6 0 12 121 10 779.6 0 10 122 14 779.6 0 14 123 10 779.6 0 10 124 15 779.6 0 15 125 37 779.6 0 37 126 21 779.6 0 21 127 13 779.6 0 13 128 9 779.6 0 9 129 27 779.6 0 27 130 13 779.6 0 13 131 7 779.6 0 7 132 10 779.6 0 10 133 15 779.6 0 15 134 19 779.6 0 19 135 26 779.6 0 26 136 14 779.6 0 14 137 11 779.6 0 11 138 8 779.6 0 8 139 15 779.6 0 15 140 8 779.6 0 8 141 22 779.6 0 22 142 10 779.6 0 10 143 35 779.6 0 35 144 30 779.6 0 30 145 26 779.6 0 26 146 10 779.6 0 10 147 7 779.6 0 7 148 8 779.6 0 8 149 15 779.6 0 15 150 9 779.6 0 9 151 2 779.6 0 2 152 10 779.6 0 10 153 19 779.6 0 19 154 4 779.6 0 4 155 9 779.6 0 9 156 11 779.6 0 11 157 14 779.6 0 14 158 27 779.6 0 27 159 44 779.6 0 44 160 45 779.6 0 45 161 667 779.6 0 667 162 153 779.6 0 153 163 355 779.6 0 355 164 38 779.6 0 38 165 50 779.6 0 50 166 21 779.6 0 21 167 31 779.6 0 31 168 29 779.6 0 29 169 28 779.6 0 28 170 23 779.6 0 23 171 40 779.6 0 40 172 45 779.6 0 45 173 29 779.6 0 29 174 11 779.6 0 11 175 10 779.6 0 10 176 12 779.6 0 12 177 35 779.6 0 35 178 25 779.6 0 25 179 29 779.6 0 29 180 21 779.6 0 21 181 10 779.6 0 10 182 6 779.6 0 6 183 12 779.6 0 12 184 6 779.6 0 6 185 8 779.6 0 8 186 12 779.6 0 12 187 20 779.6 0 20 188 14 779.6 0 14 189 37 779.6 0 37 190 109 779.6 0 109 191 70 779.6 0 70 192 11 779.6 0 11 193 16 779.6 0 16 194 15 779.6 0 15 195 21 779.6 0 21 196 32 779.6 0 32 197 21 779.6 0 21 198 21 779.6 0 21 199 35 779.6 0 35 200 22 779.6 0 22 201 48 779.6 0 48 202 61 779.6 0 61 203 18 779.6 0 18 204 23 779.6 0 23 205 11 779.6 0 11 206 17 779.6 0 17 207 9 779.6 0 9 208 10 779.6 0 10 209 10 779.6 0 10 210 12 779.6 0 12 211 57 779.6 0 57 212 36 779.6 0 36 213 41 779.6 0 41 214 13 779.6 0 13 215 22 779.6 0 22 216 29 779.6 0 29 217 32 779.6 0 32 218 8 779.6 0 8 219 11 779.6 0 11 220 13 779.6 0 13 221 5 779.6 0 5 222 6 779.6 0 6 223 28 779.6 0 28 224 31 779.6 0 31 225 85 779.6 0 85 226 41 779.6 0 41 227 17 779.6 0 17 228 18 779.6 0 18 229 14 779.6 0 14 230 27 779.6 0 27 231 21 779.6 0 21 232 13 779.6 0 13 233 17 779.6 0 17 234 18 779.6 0 18 235 17 779.6 0 17 236 24 779.6 0 24 237 28 779.6 0 28 238 14 779.6 0 14 239 13 779.6 0 13 240 3 779.6 0 3 241 5 779.6 0 5 242 11 779.6 0 11 243 29 779.6 0 29 244 58 779.6 0 58 245 79 779.6 0 79 246 38 779.6 0 38 247 33 779.6 0 33 248 9 779.6 0 9 249 5 779.6 0 5 250 10 779.6 0 10 251 6 779.6 0 6 252 11 779.6 0 11 253 14 779.6 0 14 254 23 779.6 0 23 255 29 779.6 0 29 256 21 779.6 0 21 257 6 779.6 0 6 258 16 779.6 0 16 259 9 779.6 0 9 260 12 779.6 0 12 261 17 779.6 0 17 262 6 779.6 0 6 263 10 779.6 0 10 264 18 779.6 0 18 265 28 779.6 0 28 266 14 779.6 0 14 267 10 779.6 0 10 268 4 779.6 0 4 269 7 779.6 0 7 270 10 779.6 0 10 271 7 779.6 0 7 272 5 779.6 0 5 273 5 779.6 0 5 274 10 779.6 0 10 275 11 779.6 0 11 276 9 779.6 0 9 277 5 779.6 0 5 278 10 779.6 0 10 279 8 779.6 0 8 280 11 779.6 0 11 281 4 779.6 0 4 282 9 779.6 0 9 283 9 779.6 0 9 284 19 779.6 0 19 285 14 779.6 0 14 286 17 779.6 0 17 287 17 779.6 0 17 288 7 779.6 0 7 289 5 779.6 0 5 290 6 779.6 0 6 291 7 779.6 0 7 292 7 779.6 0 7 293 8 779.6 0 8 294 5 779.6 0 5 295 8 779.6 0 8 296 5 779.6 0 5 297 15 779.6 0 15 298 26 779.6 0 26 299 25 779.6 0 25 300 21 779.6 0 21 301 6 779.6 0 6 Finished in 1.50 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 28,814 Reads with adapters: 15,399 (53.4%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 15,399 (53.4%) Total basepairs processed: 7,023,251 bp Total written (filtered): 4,189,023 bp (59.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15399 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 139 0.0 3 0 133 6 20 15131 0.0 4 14865 254 11 0 1 21 56 0.0 4 13 42 1 22 3 0.0 4 2 0 1 23 63 0.0 4 54 0 1 7 1 24 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.65 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 15,399 Reads with adapters: 504 (3.3%) Reads written (passing filters): 15,399 (100.0%) Total basepairs processed: 4,189,023 bp Total written (filtered): 4,099,867 bp (97.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 504 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 67.1% C: 1.4% G: 31.0% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 3 60.2 0 3 6 1 3.8 0 1 9 18 0.1 0 0 18 10 81 0.0 1 1 80 11 55 0.0 1 0 55 12 1 0.0 1 0 1 15 1 0.0 1 0 1 18 1 0.0 1 1 19 1 0.0 1 1 202 1 0.0 1 1 228 12 0.0 1 10 2 229 1 0.0 1 1 256 2 0.0 1 2 257 311 0.0 1 288 23 258 7 0.0 1 6 1 259 3 0.0 1 3 260 4 0.0 1 3 1 268 1 0.0 1 1