This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 100.69 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 8,860 (0.3%) Reads that were too short: 3,834 (0.1%) Reads written (passing filters): 5,026 (0.2%) Total basepairs processed: 958,672,960 bp Total written (filtered): 1,036,286 bp (0.1%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 8860 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 89 777.6 0 89 7 190 777.6 0 190 8 55 777.6 0 55 9 64 777.6 0 64 10 11 777.6 0 11 11 15 777.6 0 15 12 9 777.6 0 9 13 5 777.6 0 5 14 16 777.6 0 16 15 3 777.6 0 3 16 8 777.6 0 8 17 6 777.6 0 6 18 7 777.6 0 7 19 13 777.6 0 13 20 13 777.6 0 13 21 12 777.6 0 12 22 10 777.6 0 10 23 11 777.6 0 11 24 7 777.6 0 7 25 6 777.6 0 6 26 10 777.6 0 10 27 6 777.6 0 6 28 13 777.6 0 13 29 13 777.6 0 13 30 13 777.6 0 13 31 11 777.6 0 11 32 10 777.6 0 10 33 16 777.6 0 16 34 51 777.6 0 51 35 54 777.6 0 54 36 15 777.6 0 15 37 10 777.6 0 10 38 49 777.6 0 49 39 17 777.6 0 17 40 18 777.6 0 18 41 20 777.6 0 20 42 7 777.6 0 7 43 21 777.6 0 21 44 21 777.6 0 21 45 13 777.6 0 13 46 14 777.6 0 14 47 219 777.6 0 219 48 405 777.6 0 405 49 258 777.6 0 258 50 489 777.6 0 489 51 15 777.6 0 15 52 8 777.6 0 8 53 4 777.6 0 4 54 5 777.6 0 5 55 12 777.6 0 12 56 8 777.6 0 8 57 7 777.6 0 7 58 6 777.6 0 6 59 7 777.6 0 7 60 7 777.6 0 7 61 4 777.6 0 4 62 3 777.6 0 3 63 4 777.6 0 4 64 4 777.6 0 4 65 2 777.6 0 2 66 5 777.6 0 5 67 3 777.6 0 3 68 10 777.6 0 10 69 2 777.6 0 2 70 5 777.6 0 5 71 3 777.6 0 3 72 6 777.6 0 6 73 4 777.6 0 4 74 7 777.6 0 7 75 3 777.6 0 3 76 7 777.6 0 7 77 2 777.6 0 2 78 7 777.6 0 7 79 5 777.6 0 5 80 10 777.6 0 10 81 17 777.6 0 17 82 14 777.6 0 14 83 45 777.6 0 45 84 13 777.6 0 13 85 53 777.6 0 53 86 12 777.6 0 12 87 8 777.6 0 8 88 23 777.6 0 23 89 32 777.6 0 32 90 13 777.6 0 13 91 7 777.6 0 7 92 12 777.6 0 12 93 8 777.6 0 8 94 8 777.6 0 8 95 26 777.6 0 26 96 199 777.6 0 199 97 15 777.6 0 15 98 12 777.6 0 12 99 2 777.6 0 2 100 8 777.6 0 8 101 10 777.6 0 10 102 7 777.6 0 7 103 4 777.6 0 4 104 1 777.6 0 1 105 12 777.6 0 12 106 33 777.6 0 33 107 5 777.6 0 5 108 18 777.6 0 18 109 2 777.6 0 2 110 2 777.6 0 2 111 9 777.6 0 9 112 17 777.6 0 17 113 8 777.6 0 8 114 14 777.6 0 14 115 7 777.6 0 7 116 2 777.6 0 2 117 4 777.6 0 4 118 31 777.6 0 31 119 22 777.6 0 22 120 30 777.6 0 30 121 4 777.6 0 4 122 6 777.6 0 6 123 5 777.6 0 5 124 6 777.6 0 6 125 11 777.6 0 11 126 9 777.6 0 9 127 9 777.6 0 9 128 10 777.6 0 10 129 4 777.6 0 4 130 9 777.6 0 9 131 7 777.6 0 7 132 17 777.6 0 17 133 9 777.6 0 9 134 13 777.6 0 13 135 12 777.6 0 12 136 28 777.6 0 28 137 15 777.6 0 15 138 35 777.6 0 35 139 16 777.6 0 16 140 12 777.6 0 12 141 9 777.6 0 9 142 8 777.6 0 8 143 7 777.6 0 7 144 20 777.6 0 20 145 34 777.6 0 34 146 13 777.6 0 13 147 38 777.6 0 38 148 80 777.6 0 80 149 38 777.6 0 38 150 12 777.6 0 12 151 21 777.6 0 21 152 6 777.6 0 6 153 6 777.6 0 6 154 100 777.6 0 100 155 7 777.6 0 7 156 4 777.6 0 4 157 4 777.6 0 4 158 11 777.6 0 11 159 11 777.6 0 11 160 6 777.6 0 6 161 9 777.6 0 9 162 5 777.6 0 5 163 5 777.6 0 5 164 13 777.6 0 13 165 8 777.6 0 8 166 8 777.6 0 8 167 14 777.6 0 14 168 16 777.6 0 16 169 11 777.6 0 11 170 7 777.6 0 7 171 11 777.6 0 11 172 2 777.6 0 2 173 13 777.6 0 13 174 10 777.6 0 10 175 13 777.6 0 13 176 5 777.6 0 5 177 4 777.6 0 4 178 8 777.6 0 8 179 13 777.6 0 13 180 12 777.6 0 12 181 38 777.6 0 38 182 30 777.6 0 30 183 21 777.6 0 21 184 5 777.6 0 5 185 16 777.6 0 16 186 16 777.6 0 16 187 12 777.6 0 12 188 7 777.6 0 7 189 6 777.6 0 6 190 12 777.6 0 12 191 12 777.6 0 12 192 12 777.6 0 12 193 119 777.6 0 119 194 46 777.6 0 46 195 151 777.6 0 151 196 125 777.6 0 125 197 224 777.6 0 224 198 20 777.6 0 20 199 20 777.6 0 20 200 7 777.6 0 7 201 5 777.6 0 5 202 5 777.6 0 5 203 2 777.6 0 2 204 3 777.6 0 3 205 12 777.6 0 12 206 9 777.6 0 9 207 6 777.6 0 6 208 8 777.6 0 8 209 1 777.6 0 1 210 10 777.6 0 10 211 4 777.6 0 4 212 13 777.6 0 13 213 23 777.6 0 23 214 34 777.6 0 34 215 42 777.6 0 42 216 39 777.6 0 39 217 34 777.6 0 34 218 46 777.6 0 46 219 43 777.6 0 43 220 9 777.6 0 9 221 4 777.6 0 4 222 4 777.6 0 4 223 11 777.6 0 11 224 6 777.6 0 6 225 15 777.6 0 15 226 19 777.6 0 19 227 26 777.6 0 26 228 34 777.6 0 34 229 29 777.6 0 29 230 67 777.6 0 67 231 26 777.6 0 26 232 6 777.6 0 6 233 16 777.6 0 16 234 24 777.6 0 24 235 20 777.6 0 20 236 34 777.6 0 34 237 25 777.6 0 25 238 58 777.6 0 58 239 66 777.6 0 66 240 63 777.6 0 63 241 9 777.6 0 9 242 6 777.6 0 6 243 38 777.6 0 38 244 86 777.6 0 86 245 199 777.6 0 199 246 79 777.6 0 79 247 44 777.6 0 44 248 36 777.6 0 36 249 17 777.6 0 17 250 9 777.6 0 9 251 22 777.6 0 22 252 18 777.6 0 18 253 30 777.6 0 30 254 67 777.6 0 67 255 47 777.6 0 47 256 57 777.6 0 57 257 64 777.6 0 64 258 68 777.6 0 68 259 92 777.6 0 92 260 96 777.6 0 96 261 21 777.6 0 21 262 7 777.6 0 7 263 19 777.6 0 19 264 41 777.6 0 41 265 73 777.6 0 73 266 98 777.6 0 98 267 50 777.6 0 50 268 58 777.6 0 58 269 41 777.6 0 41 270 19 777.6 0 19 271 4 777.6 0 4 272 10 777.6 0 10 273 11 777.6 0 11 274 14 777.6 0 14 275 13 777.6 0 13 276 4 777.6 0 4 277 10 777.6 0 10 278 16 777.6 0 16 279 64 777.6 0 64 280 70 777.6 0 70 281 57 777.6 0 57 282 16 777.6 0 16 283 30 777.6 0 30 284 134 777.6 0 134 285 42 777.6 0 42 286 27 777.6 0 27 287 70 777.6 0 70 288 100 777.6 0 100 289 191 777.6 0 191 290 47 777.6 0 47 291 21 777.6 0 21 292 56 777.6 0 56 293 153 777.6 0 153 294 53 777.6 0 53 295 41 777.6 0 41 296 33 777.6 0 33 297 27 777.6 0 27 298 27 777.6 0 27 299 25 777.6 0 25 300 38 777.6 0 38 301 23 777.6 0 23 Finished in 0.23 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 5,026 Reads with adapters: 111 (2.2%) Reads that were too short: 7 (0.1%) Reads written (passing filters): 104 (2.1%) Total basepairs processed: 1,036,286 bp Total written (filtered): 22,739 bp (2.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 111 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 17 4 0.0 3 4 18 1 0.0 3 0 0 0 1 19 8 0.0 3 6 1 0 1 20 52 0.0 3 47 2 2 1 21 1 0.0 3 0 0 1 31 4 0.0 3 0 0 0 4 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 123 4 0.0 3 0 0 0 4 126 1 0.0 3 0 0 0 1 143 13 0.0 3 0 0 0 13 146 6 0.0 3 0 0 0 6 171 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (96 us/read; 0.62 M reads/minute). === Summary === Total reads processed: 104 Reads with adapters: 4 (3.8%) Reads written (passing filters): 104 (100.0%) Total basepairs processed: 22,739 bp Total written (filtered): 22,726 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 25.0% G: 50.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 1.6 0 3 4 1 0.4 0 1