This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCTAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.38 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 235,669 (7.4%) Reads that were too short: 67,376 (2.1%) Reads written (passing filters): 168,293 (5.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 29,874,802 bp (3.1%) === Adapter 1 === Sequence: GGCTAC; Type: regular 5'; Length: 6; Trimmed: 235669 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 18 777.6 0 18 7 27 777.6 0 27 8 21 777.6 0 21 9 19 777.6 0 19 10 9 777.6 0 9 11 14 777.6 0 14 12 23 777.6 0 23 13 139 777.6 0 139 14 257 777.6 0 257 15 173 777.6 0 173 16 63 777.6 0 63 17 18 777.6 0 18 18 17 777.6 0 17 19 17 777.6 0 17 20 16 777.6 0 16 21 13 777.6 0 13 22 21 777.6 0 21 23 23 777.6 0 23 24 39 777.6 0 39 25 12 777.6 0 12 26 18 777.6 0 18 27 13 777.6 0 13 28 11 777.6 0 11 29 131 777.6 0 131 30 48 777.6 0 48 31 1036 777.6 0 1036 32 6 777.6 0 6 33 7 777.6 0 7 34 3 777.6 0 3 35 18 777.6 0 18 36 3 777.6 0 3 37 4 777.6 0 4 38 3 777.6 0 3 39 27 777.6 0 27 40 7 777.6 0 7 41 24 777.6 0 24 42 7 777.6 0 7 43 3 777.6 0 3 44 4 777.6 0 4 45 10 777.6 0 10 46 2 777.6 0 2 47 8 777.6 0 8 48 4 777.6 0 4 49 11 777.6 0 11 50 5 777.6 0 5 51 17 777.6 0 17 52 10 777.6 0 10 53 24 777.6 0 24 54 14 777.6 0 14 55 5 777.6 0 5 56 5 777.6 0 5 57 3 777.6 0 3 58 12 777.6 0 12 59 8 777.6 0 8 60 12 777.6 0 12 61 6 777.6 0 6 62 5 777.6 0 5 63 3 777.6 0 3 64 3 777.6 0 3 65 5 777.6 0 5 66 9 777.6 0 9 67 10 777.6 0 10 68 6 777.6 0 6 69 4 777.6 0 4 70 5 777.6 0 5 71 6 777.6 0 6 72 3 777.6 0 3 73 6 777.6 0 6 74 10 777.6 0 10 75 8 777.6 0 8 76 5 777.6 0 5 77 4 777.6 0 4 78 2 777.6 0 2 79 4 777.6 0 4 80 9 777.6 0 9 81 12 777.6 0 12 82 30 777.6 0 30 83 71 777.6 0 71 84 124 777.6 0 124 85 84 777.6 0 84 86 14 777.6 0 14 87 7 777.6 0 7 88 6 777.6 0 6 89 24 777.6 0 24 90 18 777.6 0 18 91 11 777.6 0 11 92 19 777.6 0 19 93 9 777.6 0 9 94 5 777.6 0 5 95 15 777.6 0 15 96 8 777.6 0 8 97 21 777.6 0 21 98 51 777.6 0 51 99 24 777.6 0 24 100 158 777.6 0 158 101 47 777.6 0 47 102 52 777.6 0 52 103 17 777.6 0 17 104 115 777.6 0 115 105 55 777.6 0 55 106 118 777.6 0 118 107 192 777.6 0 192 108 313 777.6 0 313 109 412 777.6 0 412 110 569 777.6 0 569 111 1301 777.6 0 1301 112 163 777.6 0 163 113 226 777.6 0 226 114 506 777.6 0 506 115 364 777.6 0 364 116 296 777.6 0 296 117 569 777.6 0 569 118 437 777.6 0 437 119 542 777.6 0 542 120 839 777.6 0 839 121 1022 777.6 0 1022 122 985 777.6 0 985 123 2345 777.6 0 2345 124 17700 777.6 0 17700 125 123730 777.6 0 123730 126 8828 777.6 0 8828 127 67 777.6 0 67 128 147 777.6 0 147 129 26 777.6 0 26 130 14 777.6 0 14 131 19 777.6 0 19 132 5 777.6 0 5 133 19 777.6 0 19 134 11 777.6 0 11 135 27 777.6 0 27 136 6 777.6 0 6 137 1 777.6 0 1 138 4 777.6 0 4 139 9 777.6 0 9 140 15 777.6 0 15 141 16 777.6 0 16 142 11 777.6 0 11 143 9 777.6 0 9 144 12 777.6 0 12 145 4 777.6 0 4 146 22 777.6 0 22 147 169 777.6 0 169 148 90 777.6 0 90 149 80 777.6 0 80 150 10 777.6 0 10 151 29 777.6 0 29 152 120 777.6 0 120 153 90 777.6 0 90 154 132 777.6 0 132 155 28 777.6 0 28 156 41 777.6 0 41 157 66 777.6 0 66 158 99 777.6 0 99 159 159 777.6 0 159 160 67 777.6 0 67 161 50 777.6 0 50 162 61 777.6 0 61 163 27 777.6 0 27 164 23 777.6 0 23 165 5 777.6 0 5 166 12 777.6 0 12 167 15 777.6 0 15 168 28 777.6 0 28 169 6 777.6 0 6 170 31 777.6 0 31 171 13 777.6 0 13 172 29 777.6 0 29 173 28 777.6 0 28 174 37 777.6 0 37 175 7 777.6 0 7 176 43 777.6 0 43 177 404 777.6 0 404 178 241 777.6 0 241 179 403 777.6 0 403 180 45 777.6 0 45 181 11 777.6 0 11 182 6 777.6 0 6 183 8 777.6 0 8 184 10 777.6 0 10 185 6 777.6 0 6 186 7 777.6 0 7 187 4 777.6 0 4 188 1 777.6 0 1 189 1 777.6 0 1 190 11 777.6 0 11 191 4 777.6 0 4 192 9 777.6 0 9 193 7 777.6 0 7 194 10 777.6 0 10 195 10 777.6 0 10 196 3 777.6 0 3 197 8 777.6 0 8 198 1 777.6 0 1 199 7 777.6 0 7 200 5 777.6 0 5 201 3 777.6 0 3 202 5 777.6 0 5 203 5 777.6 0 5 204 2 777.6 0 2 205 9 777.6 0 9 206 10 777.6 0 10 207 14 777.6 0 14 208 6 777.6 0 6 209 11 777.6 0 11 210 3 777.6 0 3 211 7 777.6 0 7 212 8 777.6 0 8 213 8 777.6 0 8 214 24 777.6 0 24 215 10 777.6 0 10 216 11 777.6 0 11 217 7 777.6 0 7 218 6 777.6 0 6 219 6 777.6 0 6 220 23 777.6 0 23 221 8 777.6 0 8 222 4 777.6 0 4 223 5 777.6 0 5 224 11 777.6 0 11 225 5 777.6 0 5 226 11 777.6 0 11 227 13 777.6 0 13 228 15 777.6 0 15 229 17 777.6 0 17 230 32 777.6 0 32 231 19 777.6 0 19 232 31 777.6 0 31 233 360 777.6 0 360 234 89 777.6 0 89 235 70 777.6 0 70 236 127 777.6 0 127 237 8 777.6 0 8 238 7 777.6 0 7 239 11 777.6 0 11 240 14 777.6 0 14 241 22 777.6 0 22 242 14 777.6 0 14 243 23 777.6 0 23 244 38 777.6 0 38 245 17 777.6 0 17 246 21 777.6 0 21 247 36 777.6 0 36 248 17 777.6 0 17 249 11 777.6 0 11 250 11 777.6 0 11 251 20 777.6 0 20 252 37 777.6 0 37 253 10 777.6 0 10 254 34 777.6 0 34 255 46 777.6 0 46 256 80 777.6 0 80 257 1292 777.6 0 1292 258 361 777.6 0 361 259 1264 777.6 0 1264 260 76 777.6 0 76 261 100 777.6 0 100 262 194 777.6 0 194 263 69 777.6 0 69 264 72 777.6 0 72 265 89 777.6 0 89 266 18 777.6 0 18 267 28 777.6 0 28 268 30 777.6 0 30 269 50 777.6 0 50 270 31 777.6 0 31 271 6 777.6 0 6 272 5 777.6 0 5 273 7 777.6 0 7 274 10 777.6 0 10 275 28 777.6 0 28 276 26 777.6 0 26 277 7 777.6 0 7 278 5 777.6 0 5 279 10 777.6 0 10 280 55 777.6 0 55 281 42 777.6 0 42 282 13 777.6 0 13 283 13 777.6 0 13 284 7 777.6 0 7 285 13 777.6 0 13 286 16 777.6 0 16 287 66 777.6 0 66 288 271 777.6 0 271 289 249 777.6 0 249 290 137 777.6 0 137 291 38 777.6 0 38 292 164 777.6 0 164 293 14696 777.6 0 14696 294 29092 777.6 0 29092 295 15126 777.6 0 15126 296 2089 777.6 0 2089 297 15 777.6 0 15 298 5 777.6 0 5 299 6 777.6 0 6 300 9 777.6 0 9 301 7 777.6 0 7 Finished in 7.92 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 168,293 Reads with adapters: 9 (0.0%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 6 (0.0%) Total basepairs processed: 29,874,802 bp Total written (filtered): 1,539 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 4 0.0 4 4 42 1 0.0 4 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 106 1 0.0 4 1 113 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (1667 us/read; 0.04 M reads/minute). === Summary === Total reads processed: 6 Reads with adapters: 1 (16.7%) Reads written (passing filters): 6 (100.0%) Total basepairs processed: 1,539 bp Total written (filtered): 1,301 bp (84.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 238 1 0.0 1 1