This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.78 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 20,353 (0.6%) Reads that were too short: 10,849 (0.3%) Reads written (passing filters): 9,504 (0.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 1,996,159 bp (0.2%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 20353 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1004 777.6 0 1004 7 41 777.6 0 41 8 34 777.6 0 34 9 13 777.6 0 13 10 11 777.6 0 11 11 3 777.6 0 3 12 12 777.6 0 12 13 6 777.6 0 6 14 21 777.6 0 21 15 12 777.6 0 12 16 12 777.6 0 12 17 115 777.6 0 115 18 49 777.6 0 49 19 43 777.6 0 43 20 9 777.6 0 9 21 15 777.6 0 15 22 10 777.6 0 10 23 12 777.6 0 12 24 11 777.6 0 11 25 15 777.6 0 15 26 12 777.6 0 12 27 9 777.6 0 9 28 11 777.6 0 11 29 13 777.6 0 13 30 18 777.6 0 18 31 12 777.6 0 12 32 10 777.6 0 10 33 13 777.6 0 13 34 7 777.6 0 7 35 16 777.6 0 16 36 9 777.6 0 9 37 10 777.6 0 10 38 5 777.6 0 5 39 15 777.6 0 15 40 14 777.6 0 14 41 12 777.6 0 12 42 31 777.6 0 31 43 9 777.6 0 9 44 6 777.6 0 6 45 5 777.6 0 5 46 14 777.6 0 14 47 16 777.6 0 16 48 94 777.6 0 94 49 67 777.6 0 67 50 20 777.6 0 20 51 23 777.6 0 23 52 17 777.6 0 17 53 37 777.6 0 37 54 17 777.6 0 17 55 32 777.6 0 32 56 18 777.6 0 18 57 40 777.6 0 40 58 20 777.6 0 20 59 23 777.6 0 23 60 84 777.6 0 84 61 56 777.6 0 56 62 29 777.6 0 29 63 37 777.6 0 37 64 44 777.6 0 44 65 19 777.6 0 19 66 18 777.6 0 18 67 46 777.6 0 46 68 21 777.6 0 21 69 22 777.6 0 22 70 28 777.6 0 28 71 21 777.6 0 21 72 53 777.6 0 53 73 631 777.6 0 631 74 391 777.6 0 391 75 480 777.6 0 480 76 30 777.6 0 30 77 532 777.6 0 532 78 474 777.6 0 474 79 452 777.6 0 452 80 13 777.6 0 13 81 7 777.6 0 7 82 9 777.6 0 9 83 17 777.6 0 17 84 12 777.6 0 12 85 6 777.6 0 6 86 10 777.6 0 10 87 14 777.6 0 14 88 12 777.6 0 12 89 11 777.6 0 11 90 14 777.6 0 14 91 13 777.6 0 13 92 15 777.6 0 15 93 22 777.6 0 22 94 18 777.6 0 18 95 8 777.6 0 8 96 13 777.6 0 13 97 11 777.6 0 11 98 7 777.6 0 7 99 15 777.6 0 15 100 7 777.6 0 7 101 4 777.6 0 4 102 6 777.6 0 6 103 9 777.6 0 9 104 7 777.6 0 7 105 5 777.6 0 5 106 11 777.6 0 11 107 72 777.6 0 72 108 67 777.6 0 67 109 8 777.6 0 8 110 8 777.6 0 8 111 3 777.6 0 3 112 15 777.6 0 15 113 88 777.6 0 88 114 15 777.6 0 15 115 17 777.6 0 17 116 62 777.6 0 62 117 9 777.6 0 9 118 8 777.6 0 8 119 10 777.6 0 10 120 15 777.6 0 15 121 18 777.6 0 18 122 15 777.6 0 15 123 17 777.6 0 17 124 20 777.6 0 20 125 9 777.6 0 9 126 14 777.6 0 14 127 166 777.6 0 166 128 111 777.6 0 111 129 79 777.6 0 79 130 158 777.6 0 158 131 10 777.6 0 10 132 17 777.6 0 17 133 29 777.6 0 29 134 64 777.6 0 64 135 48 777.6 0 48 136 200 777.6 0 200 137 194 777.6 0 194 138 20 777.6 0 20 139 19 777.6 0 19 140 9 777.6 0 9 141 11 777.6 0 11 142 16 777.6 0 16 143 11 777.6 0 11 144 16 777.6 0 16 145 18 777.6 0 18 146 426 777.6 0 426 147 120 777.6 0 120 148 18 777.6 0 18 149 39 777.6 0 39 150 45 777.6 0 45 151 17 777.6 0 17 152 6 777.6 0 6 153 10 777.6 0 10 154 12 777.6 0 12 155 18 777.6 0 18 156 35 777.6 0 35 157 103 777.6 0 103 158 19 777.6 0 19 159 22 777.6 0 22 160 21 777.6 0 21 161 31 777.6 0 31 162 24 777.6 0 24 163 10 777.6 0 10 164 42 777.6 0 42 165 65 777.6 0 65 166 93 777.6 0 93 167 18 777.6 0 18 168 22 777.6 0 22 169 12 777.6 0 12 170 21 777.6 0 21 171 10 777.6 0 10 172 8 777.6 0 8 173 7 777.6 0 7 174 9 777.6 0 9 175 5 777.6 0 5 176 4 777.6 0 4 177 6 777.6 0 6 178 11 777.6 0 11 179 20 777.6 0 20 180 12 777.6 0 12 181 25 777.6 0 25 182 19 777.6 0 19 183 23 777.6 0 23 184 111 777.6 0 111 185 66 777.6 0 66 186 73 777.6 0 73 187 12 777.6 0 12 188 10 777.6 0 10 189 6 777.6 0 6 190 11 777.6 0 11 191 11 777.6 0 11 192 15 777.6 0 15 193 9 777.6 0 9 194 6 777.6 0 6 195 19 777.6 0 19 196 52 777.6 0 52 197 75 777.6 0 75 198 12 777.6 0 12 199 11 777.6 0 11 200 10 777.6 0 10 201 14 777.6 0 14 202 9 777.6 0 9 203 6 777.6 0 6 204 19 777.6 0 19 205 13 777.6 0 13 206 32 777.6 0 32 207 9 777.6 0 9 208 14 777.6 0 14 209 32 777.6 0 32 210 23 777.6 0 23 211 11 777.6 0 11 212 22 777.6 0 22 213 21 777.6 0 21 214 18 777.6 0 18 215 56 777.6 0 56 216 36 777.6 0 36 217 158 777.6 0 158 218 21 777.6 0 21 219 46 777.6 0 46 220 15 777.6 0 15 221 24 777.6 0 24 222 5 777.6 0 5 223 9 777.6 0 9 224 33 777.6 0 33 225 42 777.6 0 42 226 19 777.6 0 19 227 16 777.6 0 16 228 16 777.6 0 16 229 15 777.6 0 15 230 24 777.6 0 24 231 25 777.6 0 25 232 46 777.6 0 46 233 51 777.6 0 51 234 88 777.6 0 88 235 132 777.6 0 132 236 2438 777.6 0 2438 237 3214 777.6 0 3214 238 1728 777.6 0 1728 239 17 777.6 0 17 240 6 777.6 0 6 241 9 777.6 0 9 242 2 777.6 0 2 243 6 777.6 0 6 244 12 777.6 0 12 245 26 777.6 0 26 246 25 777.6 0 25 247 39 777.6 0 39 248 60 777.6 0 60 249 5 777.6 0 5 250 34 777.6 0 34 251 14 777.6 0 14 252 78 777.6 0 78 253 59 777.6 0 59 254 139 777.6 0 139 255 131 777.6 0 131 256 25 777.6 0 25 257 7 777.6 0 7 258 9 777.6 0 9 259 5 777.6 0 5 260 6 777.6 0 6 261 8 777.6 0 8 262 3 777.6 0 3 263 6 777.6 0 6 264 13 777.6 0 13 265 19 777.6 0 19 266 19 777.6 0 19 267 7 777.6 0 7 268 9 777.6 0 9 269 6 777.6 0 6 270 11 777.6 0 11 271 13 777.6 0 13 272 12 777.6 0 12 273 18 777.6 0 18 274 12 777.6 0 12 275 8 777.6 0 8 276 12 777.6 0 12 277 17 777.6 0 17 278 49 777.6 0 49 279 39 777.6 0 39 280 52 777.6 0 52 281 69 777.6 0 69 282 131 777.6 0 131 283 87 777.6 0 87 284 54 777.6 0 54 285 75 777.6 0 75 286 204 777.6 0 204 287 9 777.6 0 9 288 10 777.6 0 10 289 10 777.6 0 10 290 14 777.6 0 14 291 30 777.6 0 30 292 18 777.6 0 18 293 15 777.6 0 15 294 23 777.6 0 23 295 34 777.6 0 34 296 108 777.6 0 108 297 173 777.6 0 173 298 201 777.6 0 201 299 29 777.6 0 29 300 16 777.6 0 16 301 6 777.6 0 6 Finished in 0.44 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 9,504 Reads with adapters: 81 (0.9%) Reads that were too short: 15 (0.2%) Reads written (passing filters): 66 (0.7%) Total basepairs processed: 1,996,159 bp Total written (filtered): 8,149 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 81 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 2 1 19 1 0.0 3 1 26 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 114 33 0.0 3 0 0 0 33 115 4 0.0 3 0 0 0 4 117 4 0.0 3 0 0 0 4 118 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 4 0.0 3 0 0 0 4 218 2 0.0 3 0 0 0 2 251 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (152 us/read; 0.40 M reads/minute). === Summary === Total reads processed: 66 Reads with adapters: 0 (0.0%) Reads written (passing filters): 66 (100.0%) Total basepairs processed: 8,149 bp Total written (filtered): 8,149 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.