This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.29 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 239,999 (7.5%) Reads that were too short: 147,220 (4.6%) Reads written (passing filters): 92,779 (2.9%) Total basepairs processed: 958,672,960 bp Total written (filtered): 15,203,756 bp (1.6%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 239999 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1699 777.6 0 1699 7 59 777.6 0 59 8 2 777.6 0 2 9 49 777.6 0 49 10 17 777.6 0 17 11 4 777.6 0 4 12 22 777.6 0 22 13 6 777.6 0 6 14 1 777.6 0 1 15 3 777.6 0 3 16 2 777.6 0 2 17 5 777.6 0 5 18 4 777.6 0 4 19 3 777.6 0 3 20 4 777.6 0 4 21 4 777.6 0 4 22 2 777.6 0 2 23 1 777.6 0 1 24 2 777.6 0 2 25 5 777.6 0 5 26 4 777.6 0 4 27 6 777.6 0 6 28 4 777.6 0 4 29 4 777.6 0 4 30 4 777.6 0 4 31 5 777.6 0 5 32 5 777.6 0 5 33 3 777.6 0 3 34 5 777.6 0 5 35 6 777.6 0 6 36 5 777.6 0 5 37 4 777.6 0 4 38 3 777.6 0 3 39 4 777.6 0 4 40 4 777.6 0 4 41 58 777.6 0 58 42 31 777.6 0 31 43 7 777.6 0 7 44 1 777.6 0 1 45 2 777.6 0 2 46 2 777.6 0 2 47 2 777.6 0 2 48 7 777.6 0 7 49 2 777.6 0 2 50 8 777.6 0 8 51 2 777.6 0 2 52 5 777.6 0 5 53 2 777.6 0 2 54 84 777.6 0 84 55 2513 777.6 0 2513 56 27 777.6 0 27 57 3 777.6 0 3 58 8 777.6 0 8 59 29 777.6 0 29 60 4 777.6 0 4 61 25 777.6 0 25 62 6 777.6 0 6 63 3 777.6 0 3 64 5 777.6 0 5 65 3 777.6 0 3 66 28 777.6 0 28 67 7 777.6 0 7 68 6 777.6 0 6 69 10 777.6 0 10 70 27 777.6 0 27 71 30 777.6 0 30 72 147 777.6 0 147 73 31 777.6 0 31 74 66 777.6 0 66 75 93 777.6 0 93 76 391 777.6 0 391 77 206 777.6 0 206 78 197 777.6 0 197 79 321 777.6 0 321 80 362 777.6 0 362 81 528 777.6 0 528 82 844 777.6 0 844 83 536 777.6 0 536 84 398 777.6 0 398 85 319 777.6 0 319 86 859 777.6 0 859 87 490 777.6 0 490 88 229 777.6 0 229 89 1362 777.6 0 1362 90 147 777.6 0 147 91 236 777.6 0 236 92 828 777.6 0 828 93 392 777.6 0 392 94 263 777.6 0 263 95 197 777.6 0 197 96 124 777.6 0 124 97 1112 777.6 0 1112 98 777 777.6 0 777 99 583 777.6 0 583 100 258 777.6 0 258 101 180 777.6 0 180 102 525 777.6 0 525 103 1736 777.6 0 1736 104 515 777.6 0 515 105 252 777.6 0 252 106 389 777.6 0 389 107 193 777.6 0 193 108 1052 777.6 0 1052 109 1316 777.6 0 1316 110 906 777.6 0 906 111 489 777.6 0 489 112 581 777.6 0 581 113 664 777.6 0 664 114 496 777.6 0 496 115 1134 777.6 0 1134 116 346 777.6 0 346 117 389 777.6 0 389 118 1134 777.6 0 1134 119 404 777.6 0 404 120 487 777.6 0 487 121 566 777.6 0 566 122 444 777.6 0 444 123 500 777.6 0 500 124 528 777.6 0 528 125 850 777.6 0 850 126 551 777.6 0 551 127 827 777.6 0 827 128 624 777.6 0 624 129 621 777.6 0 621 130 688 777.6 0 688 131 387 777.6 0 387 132 756 777.6 0 756 133 1271 777.6 0 1271 134 1192 777.6 0 1192 135 1134 777.6 0 1134 136 785 777.6 0 785 137 843 777.6 0 843 138 1375 777.6 0 1375 139 1118 777.6 0 1118 140 738 777.6 0 738 141 813 777.6 0 813 142 346 777.6 0 346 143 648 777.6 0 648 144 1435 777.6 0 1435 145 715 777.6 0 715 146 1027 777.6 0 1027 147 940 777.6 0 940 148 670 777.6 0 670 149 803 777.6 0 803 150 608 777.6 0 608 151 1562 777.6 0 1562 152 766 777.6 0 766 153 1163 777.6 0 1163 154 992 777.6 0 992 155 593 777.6 0 593 156 1568 777.6 0 1568 157 912 777.6 0 912 158 784 777.6 0 784 159 913 777.6 0 913 160 636 777.6 0 636 161 794 777.6 0 794 162 547 777.6 0 547 163 532 777.6 0 532 164 861 777.6 0 861 165 644 777.6 0 644 166 640 777.6 0 640 167 375 777.6 0 375 168 489 777.6 0 489 169 293 777.6 0 293 170 592 777.6 0 592 171 567 777.6 0 567 172 1099 777.6 0 1099 173 765 777.6 0 765 174 382 777.6 0 382 175 293 777.6 0 293 176 571 777.6 0 571 177 855 777.6 0 855 178 625 777.6 0 625 179 966 777.6 0 966 180 1119 777.6 0 1119 181 777 777.6 0 777 182 772 777.6 0 772 183 502 777.6 0 502 184 716 777.6 0 716 185 609 777.6 0 609 186 665 777.6 0 665 187 600 777.6 0 600 188 985 777.6 0 985 189 1154 777.6 0 1154 190 1500 777.6 0 1500 191 643 777.6 0 643 192 502 777.6 0 502 193 291 777.6 0 291 194 618 777.6 0 618 195 696 777.6 0 696 196 768 777.6 0 768 197 958 777.6 0 958 198 438 777.6 0 438 199 541 777.6 0 541 200 845 777.6 0 845 201 1107 777.6 0 1107 202 1137 777.6 0 1137 203 685 777.6 0 685 204 497 777.6 0 497 205 600 777.6 0 600 206 1026 777.6 0 1026 207 683 777.6 0 683 208 889 777.6 0 889 209 1598 777.6 0 1598 210 1091 777.6 0 1091 211 7846 777.6 0 7846 212 3935 777.6 0 3935 213 10655 777.6 0 10655 214 4086 777.6 0 4086 215 763 777.6 0 763 216 719 777.6 0 719 217 676 777.6 0 676 218 543 777.6 0 543 219 890 777.6 0 890 220 1277 777.6 0 1277 221 652 777.6 0 652 222 867 777.6 0 867 223 1101 777.6 0 1101 224 871 777.6 0 871 225 724 777.6 0 724 226 875 777.6 0 875 227 566 777.6 0 566 228 519 777.6 0 519 229 512 777.6 0 512 230 770 777.6 0 770 231 891 777.6 0 891 232 833 777.6 0 833 233 898 777.6 0 898 234 1217 777.6 0 1217 235 673 777.6 0 673 236 683 777.6 0 683 237 892 777.6 0 892 238 931 777.6 0 931 239 1090 777.6 0 1090 240 1481 777.6 0 1481 241 1188 777.6 0 1188 242 874 777.6 0 874 243 870 777.6 0 870 244 676 777.6 0 676 245 944 777.6 0 944 246 753 777.6 0 753 247 580 777.6 0 580 248 472 777.6 0 472 249 740 777.6 0 740 250 1027 777.6 0 1027 251 1122 777.6 0 1122 252 675 777.6 0 675 253 642 777.6 0 642 254 669 777.6 0 669 255 621 777.6 0 621 256 537 777.6 0 537 257 500 777.6 0 500 258 500 777.6 0 500 259 778 777.6 0 778 260 1014 777.6 0 1014 261 866 777.6 0 866 262 1359 777.6 0 1359 263 1047 777.6 0 1047 264 1323 777.6 0 1323 265 980 777.6 0 980 266 572 777.6 0 572 267 870 777.6 0 870 268 611 777.6 0 611 269 1227 777.6 0 1227 270 3034 777.6 0 3034 271 2475 777.6 0 2475 272 8292 777.6 0 8292 273 1078 777.6 0 1078 274 957 777.6 0 957 275 779 777.6 0 779 276 1090 777.6 0 1090 277 1379 777.6 0 1379 278 1464 777.6 0 1464 279 1715 777.6 0 1715 280 791 777.6 0 791 281 531 777.6 0 531 282 866 777.6 0 866 283 1002 777.6 0 1002 284 842 777.6 0 842 285 1735 777.6 0 1735 286 1165 777.6 0 1165 287 1141 777.6 0 1141 288 1058 777.6 0 1058 289 988 777.6 0 988 290 1407 777.6 0 1407 291 1257 777.6 0 1257 292 2566 777.6 0 2566 293 3389 777.6 0 3389 294 16278 777.6 0 16278 295 1610 777.6 0 1610 296 1174 777.6 0 1174 297 1850 777.6 0 1850 298 1827 777.6 0 1827 299 823 777.6 0 823 300 823 777.6 0 823 301 1155 777.6 0 1155 Finished in 3.47 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 92,779 Reads with adapters: 71 (0.1%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 47 (0.1%) Total basepairs processed: 15,203,756 bp Total written (filtered): 6,393 bp (0.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 71 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 1 20 2 0.0 3 0 0 1 1 21 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 1 108 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 1 120 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 158 5 0.0 3 0 0 0 5 171 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (213 us/read; 0.28 M reads/minute). === Summary === Total reads processed: 47 Reads with adapters: 2 (4.3%) Reads written (passing filters): 47 (100.0%) Total basepairs processed: 6,393 bp Total written (filtered): 6,387 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 0.7 0 2