This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.62 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 17,876 (0.6%) Reads that were too short: 9,656 (0.3%) Reads written (passing filters): 8,220 (0.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 1,476,723 bp (0.2%) === Adapter 1 === Sequence: CCGCTG; Type: regular 5'; Length: 6; Trimmed: 17876 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 16 777.6 0 16 7 13 777.6 0 13 8 49 777.6 0 49 9 121 777.6 0 121 10 30 777.6 0 30 11 8 777.6 0 8 12 8 777.6 0 8 13 13 777.6 0 13 14 9 777.6 0 9 15 13 777.6 0 13 16 11 777.6 0 11 17 9 777.6 0 9 18 15 777.6 0 15 19 9 777.6 0 9 20 8 777.6 0 8 21 10 777.6 0 10 22 15 777.6 0 15 23 11 777.6 0 11 24 10 777.6 0 10 25 9 777.6 0 9 26 12 777.6 0 12 27 10 777.6 0 10 28 8 777.6 0 8 29 13 777.6 0 13 30 10 777.6 0 10 31 11 777.6 0 11 32 10 777.6 0 10 33 8 777.6 0 8 34 20 777.6 0 20 35 6 777.6 0 6 36 14 777.6 0 14 37 15 777.6 0 15 38 15 777.6 0 15 39 7 777.6 0 7 40 12 777.6 0 12 41 16 777.6 0 16 42 11 777.6 0 11 43 17 777.6 0 17 44 13 777.6 0 13 45 15 777.6 0 15 46 18 777.6 0 18 47 11 777.6 0 11 48 14 777.6 0 14 49 6 777.6 0 6 50 15 777.6 0 15 51 8 777.6 0 8 52 13 777.6 0 13 53 12 777.6 0 12 54 16 777.6 0 16 55 12 777.6 0 12 56 39 777.6 0 39 57 14 777.6 0 14 58 18 777.6 0 18 59 9 777.6 0 9 60 17 777.6 0 17 61 30 777.6 0 30 62 9 777.6 0 9 63 90 777.6 0 90 64 6 777.6 0 6 65 6 777.6 0 6 66 13 777.6 0 13 67 7 777.6 0 7 68 7 777.6 0 7 69 15 777.6 0 15 70 4 777.6 0 4 71 6 777.6 0 6 72 8 777.6 0 8 73 13 777.6 0 13 74 21 777.6 0 21 75 20 777.6 0 20 76 8 777.6 0 8 77 12 777.6 0 12 78 8 777.6 0 8 79 23 777.6 0 23 80 10 777.6 0 10 81 10 777.6 0 10 82 35 777.6 0 35 83 15 777.6 0 15 84 15 777.6 0 15 85 13 777.6 0 13 86 7 777.6 0 7 87 8 777.6 0 8 88 12 777.6 0 12 89 14 777.6 0 14 90 19 777.6 0 19 91 26 777.6 0 26 92 44 777.6 0 44 93 37 777.6 0 37 94 816 777.6 0 816 95 489 777.6 0 489 96 602 777.6 0 602 97 17 777.6 0 17 98 13 777.6 0 13 99 13 777.6 0 13 100 12 777.6 0 12 101 10 777.6 0 10 102 10 777.6 0 10 103 13 777.6 0 13 104 19 777.6 0 19 105 22 777.6 0 22 106 104 777.6 0 104 107 182 777.6 0 182 108 18 777.6 0 18 109 13 777.6 0 13 110 13 777.6 0 13 111 10 777.6 0 10 112 8 777.6 0 8 113 13 777.6 0 13 114 6 777.6 0 6 115 8 777.6 0 8 116 7 777.6 0 7 117 5 777.6 0 5 118 15 777.6 0 15 119 6 777.6 0 6 120 7 777.6 0 7 121 6 777.6 0 6 122 11 777.6 0 11 123 13 777.6 0 13 124 11 777.6 0 11 125 15 777.6 0 15 126 21 777.6 0 21 127 19 777.6 0 19 128 387 777.6 0 387 129 325 777.6 0 325 130 215 777.6 0 215 131 13 777.6 0 13 132 13 777.6 0 13 133 14 777.6 0 14 134 13 777.6 0 13 135 10 777.6 0 10 136 7 777.6 0 7 137 14 777.6 0 14 138 13 777.6 0 13 139 11 777.6 0 11 140 14 777.6 0 14 141 151 777.6 0 151 142 24 777.6 0 24 143 22 777.6 0 22 144 30 777.6 0 30 145 389 777.6 0 389 146 348 777.6 0 348 147 286 777.6 0 286 148 6 777.6 0 6 149 8 777.6 0 8 150 7 777.6 0 7 151 9 777.6 0 9 152 5 777.6 0 5 153 17 777.6 0 17 154 8 777.6 0 8 155 7 777.6 0 7 156 11 777.6 0 11 157 5 777.6 0 5 158 8 777.6 0 8 159 9 777.6 0 9 160 10 777.6 0 10 161 10 777.6 0 10 162 18 777.6 0 18 163 9 777.6 0 9 164 6 777.6 0 6 165 13 777.6 0 13 166 19 777.6 0 19 167 18 777.6 0 18 168 19 777.6 0 19 169 7 777.6 0 7 170 6 777.6 0 6 171 19 777.6 0 19 172 30 777.6 0 30 173 58 777.6 0 58 174 69 777.6 0 69 175 17 777.6 0 17 176 25 777.6 0 25 177 45 777.6 0 45 178 90 777.6 0 90 179 106 777.6 0 106 180 123 777.6 0 123 181 35 777.6 0 35 182 51 777.6 0 51 183 39 777.6 0 39 184 38 777.6 0 38 185 24 777.6 0 24 186 14 777.6 0 14 187 19 777.6 0 19 188 9 777.6 0 9 189 10 777.6 0 10 190 8 777.6 0 8 191 110 777.6 0 110 192 93 777.6 0 93 193 163 777.6 0 163 194 31 777.6 0 31 195 16 777.6 0 16 196 26 777.6 0 26 197 151 777.6 0 151 198 75 777.6 0 75 199 171 777.6 0 171 200 41 777.6 0 41 201 10 777.6 0 10 202 11 777.6 0 11 203 25 777.6 0 25 204 47 777.6 0 47 205 12 777.6 0 12 206 7 777.6 0 7 207 4 777.6 0 4 208 10 777.6 0 10 209 10 777.6 0 10 210 7 777.6 0 7 211 17 777.6 0 17 212 13 777.6 0 13 213 29 777.6 0 29 214 176 777.6 0 176 215 1002 777.6 0 1002 216 217 777.6 0 217 217 161 777.6 0 161 218 8 777.6 0 8 219 6 777.6 0 6 220 15 777.6 0 15 221 4 777.6 0 4 222 12 777.6 0 12 223 5 777.6 0 5 224 9 777.6 0 9 225 2 777.6 0 2 226 6 777.6 0 6 227 9 777.6 0 9 228 9 777.6 0 9 229 4 777.6 0 4 230 5 777.6 0 5 231 13 777.6 0 13 232 5 777.6 0 5 233 11 777.6 0 11 234 11 777.6 0 11 235 12 777.6 0 12 236 6 777.6 0 6 237 7 777.6 0 7 238 10 777.6 0 10 239 10 777.6 0 10 240 2 777.6 0 2 241 7 777.6 0 7 242 7 777.6 0 7 243 11 777.6 0 11 244 6 777.6 0 6 245 9 777.6 0 9 246 15 777.6 0 15 247 13 777.6 0 13 248 203 777.6 0 203 249 14 777.6 0 14 250 112 777.6 0 112 251 8 777.6 0 8 252 12 777.6 0 12 253 43 777.6 0 43 254 9 777.6 0 9 255 22 777.6 0 22 256 24 777.6 0 24 257 23 777.6 0 23 258 14 777.6 0 14 259 22 777.6 0 22 260 5 777.6 0 5 261 8 777.6 0 8 262 18 777.6 0 18 263 12 777.6 0 12 264 13 777.6 0 13 265 23 777.6 0 23 266 21 777.6 0 21 267 13 777.6 0 13 268 29 777.6 0 29 269 24 777.6 0 24 270 12 777.6 0 12 271 116 777.6 0 116 272 17 777.6 0 17 273 44 777.6 0 44 274 7 777.6 0 7 275 13 777.6 0 13 276 11 777.6 0 11 277 23 777.6 0 23 278 50 777.6 0 50 279 191 777.6 0 191 280 67 777.6 0 67 281 68 777.6 0 68 282 9 777.6 0 9 283 8 777.6 0 8 284 23 777.6 0 23 285 39 777.6 0 39 286 56 777.6 0 56 287 75 777.6 0 75 288 158 777.6 0 158 289 1098 777.6 0 1098 290 1043 777.6 0 1043 291 755 777.6 0 755 292 510 777.6 0 510 293 496 777.6 0 496 294 568 777.6 0 568 295 645 777.6 0 645 296 549 777.6 0 549 297 283 777.6 0 283 298 39 777.6 0 39 299 5 777.6 0 5 300 7 777.6 0 7 301 2 777.6 0 2 Finished in 0.42 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 8,220 Reads with adapters: 5 (0.1%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 4 (0.0%) Total basepairs processed: 1,476,723 bp Total written (filtered): 983 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 1 0.0 4 1 21 2 0.0 4 1 1 67 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (2500 us/read; 0.02 M reads/minute). === Summary === Total reads processed: 4 Reads with adapters: 0 (0.0%) Reads written (passing filters): 4 (100.0%) Total basepairs processed: 983 bp Total written (filtered): 983 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.