This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.59 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 376,868 (11.8%) Reads that were too short: 120,690 (3.8%) Reads written (passing filters): 256,178 (8.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 70,340,527 bp (7.3%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 376868 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3483 777.6 0 3483 7 3412 777.6 0 3412 8 214946 777.6 0 214946 9 265 777.6 0 265 10 30 777.6 0 30 11 41 777.6 0 41 12 18 777.6 0 18 13 74 777.6 0 74 14 27 777.6 0 27 15 25 777.6 0 25 16 16 777.6 0 16 17 37 777.6 0 37 18 39 777.6 0 39 19 31 777.6 0 31 20 26 777.6 0 26 21 33 777.6 0 33 22 7 777.6 0 7 23 15 777.6 0 15 24 19 777.6 0 19 25 11 777.6 0 11 26 33 777.6 0 33 27 17 777.6 0 17 28 24 777.6 0 24 29 20 777.6 0 20 30 22 777.6 0 22 31 23 777.6 0 23 32 26 777.6 0 26 33 18 777.6 0 18 34 18 777.6 0 18 35 14 777.6 0 14 36 12 777.6 0 12 37 52 777.6 0 52 38 41 777.6 0 41 39 66 777.6 0 66 40 34 777.6 0 34 41 19 777.6 0 19 42 24 777.6 0 24 43 15 777.6 0 15 44 18 777.6 0 18 45 64 777.6 0 64 46 560 777.6 0 560 47 46 777.6 0 46 48 72 777.6 0 72 49 25 777.6 0 25 50 38 777.6 0 38 51 44 777.6 0 44 52 98 777.6 0 98 53 22 777.6 0 22 54 17 777.6 0 17 55 79 777.6 0 79 56 23 777.6 0 23 57 16 777.6 0 16 58 28 777.6 0 28 59 29 777.6 0 29 60 19 777.6 0 19 61 18 777.6 0 18 62 20 777.6 0 20 63 14 777.6 0 14 64 19 777.6 0 19 65 11 777.6 0 11 66 16 777.6 0 16 67 27 777.6 0 27 68 27 777.6 0 27 69 17 777.6 0 17 70 16 777.6 0 16 71 15 777.6 0 15 72 36 777.6 0 36 73 123 777.6 0 123 74 115 777.6 0 115 75 179 777.6 0 179 76 38 777.6 0 38 77 77 777.6 0 77 78 60 777.6 0 60 79 57 777.6 0 57 80 43 777.6 0 43 81 78 777.6 0 78 82 68 777.6 0 68 83 160 777.6 0 160 84 121 777.6 0 121 85 198 777.6 0 198 86 140 777.6 0 140 87 48 777.6 0 48 88 81 777.6 0 81 89 103 777.6 0 103 90 57 777.6 0 57 91 110 777.6 0 110 92 129 777.6 0 129 93 196 777.6 0 196 94 207 777.6 0 207 95 221 777.6 0 221 96 153 777.6 0 153 97 182 777.6 0 182 98 156 777.6 0 156 99 359 777.6 0 359 100 93 777.6 0 93 101 31 777.6 0 31 102 45 777.6 0 45 103 26 777.6 0 26 104 36 777.6 0 36 105 78 777.6 0 78 106 126 777.6 0 126 107 55 777.6 0 55 108 47 777.6 0 47 109 58 777.6 0 58 110 40 777.6 0 40 111 44 777.6 0 44 112 56 777.6 0 56 113 57 777.6 0 57 114 104 777.6 0 104 115 102 777.6 0 102 116 123 777.6 0 123 117 136 777.6 0 136 118 114 777.6 0 114 119 151 777.6 0 151 120 155 777.6 0 155 121 123 777.6 0 123 122 152 777.6 0 152 123 110 777.6 0 110 124 155 777.6 0 155 125 153 777.6 0 153 126 180 777.6 0 180 127 180 777.6 0 180 128 129 777.6 0 129 129 179 777.6 0 179 130 257 777.6 0 257 131 148 777.6 0 148 132 77 777.6 0 77 133 161 777.6 0 161 134 217 777.6 0 217 135 345 777.6 0 345 136 393 777.6 0 393 137 214 777.6 0 214 138 187 777.6 0 187 139 136 777.6 0 136 140 215 777.6 0 215 141 111 777.6 0 111 142 74 777.6 0 74 143 101 777.6 0 101 144 214 777.6 0 214 145 794 777.6 0 794 146 706 777.6 0 706 147 1469 777.6 0 1469 148 163 777.6 0 163 149 87 777.6 0 87 150 82 777.6 0 82 151 103 777.6 0 103 152 163 777.6 0 163 153 208 777.6 0 208 154 225 777.6 0 225 155 230 777.6 0 230 156 290 777.6 0 290 157 563 777.6 0 563 158 262 777.6 0 262 159 206 777.6 0 206 160 194 777.6 0 194 161 226 777.6 0 226 162 240 777.6 0 240 163 214 777.6 0 214 164 269 777.6 0 269 165 468 777.6 0 468 166 378 777.6 0 378 167 334 777.6 0 334 168 193 777.6 0 193 169 253 777.6 0 253 170 88 777.6 0 88 171 117 777.6 0 117 172 202 777.6 0 202 173 2878 777.6 0 2878 174 792 777.6 0 792 175 660 777.6 0 660 176 403 777.6 0 403 177 107 777.6 0 107 178 164 777.6 0 164 179 218 777.6 0 218 180 314 777.6 0 314 181 538 777.6 0 538 182 968 777.6 0 968 183 804 777.6 0 804 184 625 777.6 0 625 185 341 777.6 0 341 186 667 777.6 0 667 187 347 777.6 0 347 188 522 777.6 0 522 189 210 777.6 0 210 190 224 777.6 0 224 191 385 777.6 0 385 192 331 777.6 0 331 193 574 777.6 0 574 194 332 777.6 0 332 195 76 777.6 0 76 196 104 777.6 0 104 197 238 777.6 0 238 198 276 777.6 0 276 199 200 777.6 0 200 200 149 777.6 0 149 201 190 777.6 0 190 202 204 777.6 0 204 203 377 777.6 0 377 204 262 777.6 0 262 205 305 777.6 0 305 206 413 777.6 0 413 207 243 777.6 0 243 208 271 777.6 0 271 209 274 777.6 0 274 210 406 777.6 0 406 211 369 777.6 0 369 212 637 777.6 0 637 213 356 777.6 0 356 214 391 777.6 0 391 215 449 777.6 0 449 216 394 777.6 0 394 217 534 777.6 0 534 218 550 777.6 0 550 219 516 777.6 0 516 220 416 777.6 0 416 221 375 777.6 0 375 222 486 777.6 0 486 223 710 777.6 0 710 224 571 777.6 0 571 225 8741 777.6 0 8741 226 3983 777.6 0 3983 227 8606 777.6 0 8606 228 757 777.6 0 757 229 1159 777.6 0 1159 230 1457 777.6 0 1457 231 1571 777.6 0 1571 232 3171 777.6 0 3171 233 1015 777.6 0 1015 234 1142 777.6 0 1142 235 697 777.6 0 697 236 259 777.6 0 259 237 396 777.6 0 396 238 291 777.6 0 291 239 198 777.6 0 198 240 217 777.6 0 217 241 294 777.6 0 294 242 347 777.6 0 347 243 367 777.6 0 367 244 330 777.6 0 330 245 419 777.6 0 419 246 861 777.6 0 861 247 663 777.6 0 663 248 852 777.6 0 852 249 209 777.6 0 209 250 4573 777.6 0 4573 251 4432 777.6 0 4432 252 4634 777.6 0 4634 253 1646 777.6 0 1646 254 767 777.6 0 767 255 657 777.6 0 657 256 708 777.6 0 708 257 410 777.6 0 410 258 258 777.6 0 258 259 161 777.6 0 161 260 120 777.6 0 120 261 106 777.6 0 106 262 119 777.6 0 119 263 144 777.6 0 144 264 225 777.6 0 225 265 567 777.6 0 567 266 602 777.6 0 602 267 437 777.6 0 437 268 301 777.6 0 301 269 226 777.6 0 226 270 291 777.6 0 291 271 1151 777.6 0 1151 272 757 777.6 0 757 273 921 777.6 0 921 274 388 777.6 0 388 275 802 777.6 0 802 276 246 777.6 0 246 277 307 777.6 0 307 278 684 777.6 0 684 279 1252 777.6 0 1252 280 2390 777.6 0 2390 281 1940 777.6 0 1940 282 1454 777.6 0 1454 283 518 777.6 0 518 284 550 777.6 0 550 285 1011 777.6 0 1011 286 984 777.6 0 984 287 730 777.6 0 730 288 378 777.6 0 378 289 467 777.6 0 467 290 735 777.6 0 735 291 733 777.6 0 733 292 956 777.6 0 956 293 458 777.6 0 458 294 412 777.6 0 412 295 639 777.6 0 639 296 838 777.6 0 838 297 1162 777.6 0 1162 298 1846 777.6 0 1846 299 2502 777.6 0 2502 300 3106 777.6 0 3106 301 21478 777.6 0 21478 Finished in 11.95 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 256,178 Reads with adapters: 221,132 (86.3%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 221,098 (86.3%) Total basepairs processed: 70,340,527 bp Total written (filtered): 61,020,167 bp (86.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 221132 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 17 0.0 2 0 0 0 17 15 117 0.0 3 0 0 89 28 16 2127 0.0 3 0 1921 114 92 17 216886 0.0 3 209856 6122 704 204 18 495 0.0 3 27 403 31 34 19 1236 0.0 3 1 0 987 248 20 153 0.0 3 1 0 0 152 21 2 0.0 3 1 0 0 1 27 1 0.0 3 0 0 0 1 34 1 0.0 3 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 46 17 0.0 3 0 0 0 17 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 10 0.0 3 0 0 0 10 51 1 0.0 3 0 0 1 52 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 7 0.0 3 0 0 0 7 97 3 0.0 3 0 0 1 2 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 1 1 115 2 0.0 3 0 0 0 2 116 4 0.0 3 0 0 0 4 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 121 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 6 0.0 3 0 0 2 4 137 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.91 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 221,098 Reads with adapters: 3,595 (1.6%) Reads written (passing filters): 221,098 (100.0%) Total basepairs processed: 61,020,167 bp Total written (filtered): 61,008,839 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3595 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.4% C: 25.3% G: 25.6% T: 15.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3079 3454.7 0 3079 4 491 863.7 0 491 5 23 215.9 0 23 6 2 54.0 0 2