This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGTGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 106.18 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 270,480 (8.5%) Reads that were too short: 2,692 (0.1%) Reads written (passing filters): 267,788 (8.4%) Total basepairs processed: 958,672,960 bp Total written (filtered): 74,517,599 bp (7.8%) === Adapter 1 === Sequence: AGTGTT; Type: regular 5'; Length: 6; Trimmed: 270480 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1382 777.6 0 1382 7 8212 777.6 0 8212 8 217647 777.6 0 217647 9 311 777.6 0 311 10 25 777.6 0 25 11 13 777.6 0 13 12 13 777.6 0 13 13 8 777.6 0 8 14 12 777.6 0 12 15 11 777.6 0 11 16 10 777.6 0 10 17 9 777.6 0 9 18 7 777.6 0 7 19 10 777.6 0 10 20 11 777.6 0 11 21 8 777.6 0 8 22 13 777.6 0 13 23 8 777.6 0 8 24 12 777.6 0 12 25 14 777.6 0 14 26 11 777.6 0 11 27 12 777.6 0 12 28 10 777.6 0 10 29 8 777.6 0 8 30 18 777.6 0 18 31 11 777.6 0 11 32 20 777.6 0 20 33 6 777.6 0 6 34 14 777.6 0 14 35 15 777.6 0 15 36 10 777.6 0 10 37 12 777.6 0 12 38 18 777.6 0 18 39 14 777.6 0 14 40 15 777.6 0 15 41 42 777.6 0 42 42 25 777.6 0 25 43 9 777.6 0 9 44 15 777.6 0 15 45 13 777.6 0 13 46 15 777.6 0 15 47 13 777.6 0 13 48 15 777.6 0 15 49 16 777.6 0 16 50 16 777.6 0 16 51 38 777.6 0 38 52 18 777.6 0 18 53 27 777.6 0 27 54 15 777.6 0 15 55 32 777.6 0 32 56 39 777.6 0 39 57 29 777.6 0 29 58 34 777.6 0 34 59 17 777.6 0 17 60 23 777.6 0 23 61 41 777.6 0 41 62 275 777.6 0 275 63 156 777.6 0 156 64 152 777.6 0 152 65 91 777.6 0 91 66 155 777.6 0 155 67 252 777.6 0 252 68 4473 777.6 0 4473 69 1319 777.6 0 1319 70 6946 777.6 0 6946 71 68 777.6 0 68 72 65 777.6 0 65 73 333 777.6 0 333 74 11 777.6 0 11 75 14 777.6 0 14 76 12 777.6 0 12 77 13 777.6 0 13 78 10 777.6 0 10 79 34 777.6 0 34 80 13 777.6 0 13 81 25 777.6 0 25 82 23 777.6 0 23 83 17 777.6 0 17 84 16 777.6 0 16 85 10 777.6 0 10 86 6 777.6 0 6 87 25 777.6 0 25 88 38 777.6 0 38 89 19 777.6 0 19 90 11 777.6 0 11 91 14 777.6 0 14 92 13 777.6 0 13 93 34 777.6 0 34 94 98 777.6 0 98 95 109 777.6 0 109 96 50 777.6 0 50 97 40 777.6 0 40 98 70 777.6 0 70 99 1666 777.6 0 1666 100 2962 777.6 0 2962 101 7634 777.6 0 7634 102 22 777.6 0 22 103 9 777.6 0 9 104 11 777.6 0 11 105 11 777.6 0 11 106 10 777.6 0 10 107 7 777.6 0 7 108 14 777.6 0 14 109 11 777.6 0 11 110 23 777.6 0 23 111 12 777.6 0 12 112 17 777.6 0 17 113 11 777.6 0 11 114 15 777.6 0 15 115 14 777.6 0 14 116 12 777.6 0 12 117 19 777.6 0 19 118 28 777.6 0 28 119 16 777.6 0 16 120 20 777.6 0 20 121 25 777.6 0 25 122 12 777.6 0 12 123 16 777.6 0 16 124 28 777.6 0 28 125 41 777.6 0 41 126 25 777.6 0 25 127 32 777.6 0 32 128 49 777.6 0 49 129 63 777.6 0 63 130 57 777.6 0 57 131 299 777.6 0 299 132 1682 777.6 0 1682 133 277 777.6 0 277 134 22 777.6 0 22 135 43 777.6 0 43 136 101 777.6 0 101 137 19 777.6 0 19 138 16 777.6 0 16 139 29 777.6 0 29 140 27 777.6 0 27 141 40 777.6 0 40 142 37 777.6 0 37 143 54 777.6 0 54 144 101 777.6 0 101 145 2899 777.6 0 2899 146 288 777.6 0 288 147 294 777.6 0 294 148 11 777.6 0 11 149 18 777.6 0 18 150 7 777.6 0 7 151 9 777.6 0 9 152 7 777.6 0 7 153 7 777.6 0 7 154 10 777.6 0 10 155 7 777.6 0 7 156 9 777.6 0 9 157 13 777.6 0 13 158 13 777.6 0 13 159 13 777.6 0 13 160 11 777.6 0 11 161 6 777.6 0 6 162 10 777.6 0 10 163 15 777.6 0 15 164 10 777.6 0 10 165 19 777.6 0 19 166 18 777.6 0 18 167 73 777.6 0 73 168 46 777.6 0 46 169 57 777.6 0 57 170 20 777.6 0 20 171 12 777.6 0 12 172 12 777.6 0 12 173 12 777.6 0 12 174 25 777.6 0 25 175 20 777.6 0 20 176 10 777.6 0 10 177 20 777.6 0 20 178 13 777.6 0 13 179 20 777.6 0 20 180 26 777.6 0 26 181 29 777.6 0 29 182 34 777.6 0 34 183 29 777.6 0 29 184 44 777.6 0 44 185 49 777.6 0 49 186 35 777.6 0 35 187 33 777.6 0 33 188 27 777.6 0 27 189 26 777.6 0 26 190 30 777.6 0 30 191 71 777.6 0 71 192 121 777.6 0 121 193 184 777.6 0 184 194 152 777.6 0 152 195 405 777.6 0 405 196 200 777.6 0 200 197 508 777.6 0 508 198 1032 777.6 0 1032 199 935 777.6 0 935 200 641 777.6 0 641 201 72 777.6 0 72 202 23 777.6 0 23 203 33 777.6 0 33 204 41 777.6 0 41 205 50 777.6 0 50 206 34 777.6 0 34 207 37 777.6 0 37 208 18 777.6 0 18 209 16 777.6 0 16 210 19 777.6 0 19 211 36 777.6 0 36 212 96 777.6 0 96 213 96 777.6 0 96 214 36 777.6 0 36 215 20 777.6 0 20 216 51 777.6 0 51 217 26 777.6 0 26 218 24 777.6 0 24 219 132 777.6 0 132 220 48 777.6 0 48 221 172 777.6 0 172 222 30 777.6 0 30 223 36 777.6 0 36 224 23 777.6 0 23 225 33 777.6 0 33 226 26 777.6 0 26 227 21 777.6 0 21 228 20 777.6 0 20 229 15 777.6 0 15 230 15 777.6 0 15 231 5 777.6 0 5 232 9 777.6 0 9 233 17 777.6 0 17 234 23 777.6 0 23 235 19 777.6 0 19 236 19 777.6 0 19 237 9 777.6 0 9 238 14 777.6 0 14 239 12 777.6 0 12 240 8 777.6 0 8 241 30 777.6 0 30 242 32 777.6 0 32 243 31 777.6 0 31 244 28 777.6 0 28 245 21 777.6 0 21 246 12 777.6 0 12 247 7 777.6 0 7 248 21 777.6 0 21 249 14 777.6 0 14 250 19 777.6 0 19 251 24 777.6 0 24 252 47 777.6 0 47 253 35 777.6 0 35 254 36 777.6 0 36 255 30 777.6 0 30 256 24 777.6 0 24 257 16 777.6 0 16 258 14 777.6 0 14 259 11 777.6 0 11 260 19 777.6 0 19 261 18 777.6 0 18 262 22 777.6 0 22 263 38 777.6 0 38 264 32 777.6 0 32 265 29 777.6 0 29 266 39 777.6 0 39 267 29 777.6 0 29 268 25 777.6 0 25 269 27 777.6 0 27 270 10 777.6 0 10 271 15 777.6 0 15 272 13 777.6 0 13 273 40 777.6 0 40 274 17 777.6 0 17 275 21 777.6 0 21 276 11 777.6 0 11 277 10 777.6 0 10 278 12 777.6 0 12 279 23 777.6 0 23 280 18 777.6 0 18 281 14 777.6 0 14 282 19 777.6 0 19 283 16 777.6 0 16 284 38 777.6 0 38 285 25 777.6 0 25 286 12 777.6 0 12 287 11 777.6 0 11 288 11 777.6 0 11 289 7 777.6 0 7 290 9 777.6 0 9 291 17 777.6 0 17 292 21 777.6 0 21 293 23 777.6 0 23 294 14 777.6 0 14 295 8 777.6 0 8 296 8 777.6 0 8 297 12 777.6 0 12 298 20 777.6 0 20 299 18 777.6 0 18 300 34 777.6 0 34 301 73 777.6 0 73 Finished in 13.12 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 267,788 Reads with adapters: 227,280 (84.9%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 227,280 (84.9%) Total basepairs processed: 74,517,599 bp Total written (filtered): 62,057,837 bp (83.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 227280 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 4 0.0 3 0 0 0 0 4 17 2 0.0 3 0 0 0 0 2 18 69 0.0 3 0 0 68 0 1 19 1689 0.0 3 0 1629 22 17 21 20 224699 0.0 4 221565 3033 84 13 4 21 665 0.0 4 80 580 4 0 1 22 48 0.0 4 1 0 45 0 2 23 86 0.0 4 1 3 2 76 4 24 3 0.0 4 1 0 0 0 2 25 3 0.0 4 2 0 1 27 1 0.0 4 0 0 0 1 28 1 0.0 4 1 29 1 0.0 4 1 30 2 0.0 4 1 0 1 34 1 0.0 4 1 35 1 0.0 4 1 36 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.52 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 227,280 Reads with adapters: 3,899 (1.7%) Reads written (passing filters): 227,280 (100.0%) Total basepairs processed: 62,057,837 bp Total written (filtered): 62,012,157 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3899 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 0.9% G: 97.5% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 3551.2 0 7 4 13 887.8 0 13 5 7 222.0 0 7 6 9 55.5 0 9 9 1 0.9 0 0 1 10 87 0.2 1 0 87 11 3748 0.1 1 5 3743 12 8 0.0 1 0 8 14 1 0.0 1 0 1 15 1 0.0 1 0 1 139 1 0.0 1 1 148 1 0.0 1 1 150 1 0.0 1 1 151 3 0.0 1 0 3 170 1 0.0 1 1 209 1 0.0 1 1 224 8 0.0 1 7 1 225 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.