This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.59 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 105,021 (3.3%) Reads that were too short: 56,191 (1.8%) Reads written (passing filters): 48,830 (1.5%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,017,216 bp (1.4%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 105021 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 703 777.6 0 703 7 37642 777.6 0 37642 8 76 777.6 0 76 9 11 777.6 0 11 10 5 777.6 0 5 11 7 777.6 0 7 12 15 777.6 0 15 13 13 777.6 0 13 14 9 777.6 0 9 15 5 777.6 0 5 16 5 777.6 0 5 17 6 777.6 0 6 18 10 777.6 0 10 19 9 777.6 0 9 20 12 777.6 0 12 21 18 777.6 0 18 22 18 777.6 0 18 23 105 777.6 0 105 24 5 777.6 0 5 25 10 777.6 0 10 26 7 777.6 0 7 27 6 777.6 0 6 28 8 777.6 0 8 29 16 777.6 0 16 30 5 777.6 0 5 31 36 777.6 0 36 32 6 777.6 0 6 33 15 777.6 0 15 34 10 777.6 0 10 35 9 777.6 0 9 36 9 777.6 0 9 37 12 777.6 0 12 38 16 777.6 0 16 39 22 777.6 0 22 40 19 777.6 0 19 41 36 777.6 0 36 42 54 777.6 0 54 43 9 777.6 0 9 44 3 777.6 0 3 45 10 777.6 0 10 46 13 777.6 0 13 47 7 777.6 0 7 48 3 777.6 0 3 49 6 777.6 0 6 50 4 777.6 0 4 51 10 777.6 0 10 52 47 777.6 0 47 53 10 777.6 0 10 54 8 777.6 0 8 55 3 777.6 0 3 56 7 777.6 0 7 57 1 777.6 0 1 58 5 777.6 0 5 59 4 777.6 0 4 60 18 777.6 0 18 61 10 777.6 0 10 62 8 777.6 0 8 63 6 777.6 0 6 64 10 777.6 0 10 65 11 777.6 0 11 66 8 777.6 0 8 67 6 777.6 0 6 68 5 777.6 0 5 69 8 777.6 0 8 70 26 777.6 0 26 71 10 777.6 0 10 72 23 777.6 0 23 73 6 777.6 0 6 74 11 777.6 0 11 75 12 777.6 0 12 76 4 777.6 0 4 77 11 777.6 0 11 78 41 777.6 0 41 79 13 777.6 0 13 80 20 777.6 0 20 81 31 777.6 0 31 82 75 777.6 0 75 83 40 777.6 0 40 84 51 777.6 0 51 85 167 777.6 0 167 86 180 777.6 0 180 87 36 777.6 0 36 88 28 777.6 0 28 89 26 777.6 0 26 90 20 777.6 0 20 91 72 777.6 0 72 92 34 777.6 0 34 93 36 777.6 0 36 94 50 777.6 0 50 95 64 777.6 0 64 96 65 777.6 0 65 97 55 777.6 0 55 98 128 777.6 0 128 99 238 777.6 0 238 100 265 777.6 0 265 101 59 777.6 0 59 102 175 777.6 0 175 103 88 777.6 0 88 104 25 777.6 0 25 105 20 777.6 0 20 106 50 777.6 0 50 107 41 777.6 0 41 108 15 777.6 0 15 109 9 777.6 0 9 110 17 777.6 0 17 111 43 777.6 0 43 112 43 777.6 0 43 113 84 777.6 0 84 114 39 777.6 0 39 115 32 777.6 0 32 116 26 777.6 0 26 117 26 777.6 0 26 118 55 777.6 0 55 119 68 777.6 0 68 120 38 777.6 0 38 121 52 777.6 0 52 122 25 777.6 0 25 123 84 777.6 0 84 124 84 777.6 0 84 125 122 777.6 0 122 126 49 777.6 0 49 127 52 777.6 0 52 128 42 777.6 0 42 129 18 777.6 0 18 130 40 777.6 0 40 131 39 777.6 0 39 132 77 777.6 0 77 133 183 777.6 0 183 134 45 777.6 0 45 135 41 777.6 0 41 136 67 777.6 0 67 137 97 777.6 0 97 138 129 777.6 0 129 139 117 777.6 0 117 140 129 777.6 0 129 141 98 777.6 0 98 142 70 777.6 0 70 143 186 777.6 0 186 144 247 777.6 0 247 145 201 777.6 0 201 146 57 777.6 0 57 147 44 777.6 0 44 148 57 777.6 0 57 149 67 777.6 0 67 150 60 777.6 0 60 151 29 777.6 0 29 152 52 777.6 0 52 153 59 777.6 0 59 154 24 777.6 0 24 155 26 777.6 0 26 156 24 777.6 0 24 157 44 777.6 0 44 158 84 777.6 0 84 159 84 777.6 0 84 160 84 777.6 0 84 161 51 777.6 0 51 162 53 777.6 0 53 163 71 777.6 0 71 164 56 777.6 0 56 165 49 777.6 0 49 166 40 777.6 0 40 167 86 777.6 0 86 168 71 777.6 0 71 169 174 777.6 0 174 170 281 777.6 0 281 171 159 777.6 0 159 172 56 777.6 0 56 173 101 777.6 0 101 174 59 777.6 0 59 175 49 777.6 0 49 176 40 777.6 0 40 177 49 777.6 0 49 178 61 777.6 0 61 179 60 777.6 0 60 180 69 777.6 0 69 181 42 777.6 0 42 182 52 777.6 0 52 183 172 777.6 0 172 184 147 777.6 0 147 185 181 777.6 0 181 186 90 777.6 0 90 187 261 777.6 0 261 188 154 777.6 0 154 189 133 777.6 0 133 190 26 777.6 0 26 191 43 777.6 0 43 192 49 777.6 0 49 193 59 777.6 0 59 194 133 777.6 0 133 195 89 777.6 0 89 196 38 777.6 0 38 197 33 777.6 0 33 198 86 777.6 0 86 199 203 777.6 0 203 200 82 777.6 0 82 201 32 777.6 0 32 202 17 777.6 0 17 203 22 777.6 0 22 204 62 777.6 0 62 205 96 777.6 0 96 206 148 777.6 0 148 207 85 777.6 0 85 208 38 777.6 0 38 209 51 777.6 0 51 210 99 777.6 0 99 211 198 777.6 0 198 212 133 777.6 0 133 213 141 777.6 0 141 214 980 777.6 0 980 215 818 777.6 0 818 216 1038 777.6 0 1038 217 88 777.6 0 88 218 99 777.6 0 99 219 149 777.6 0 149 220 81 777.6 0 81 221 43 777.6 0 43 222 58 777.6 0 58 223 1744 777.6 0 1744 224 422 777.6 0 422 225 631 777.6 0 631 226 192 777.6 0 192 227 99 777.6 0 99 228 108 777.6 0 108 229 223 777.6 0 223 230 79 777.6 0 79 231 78 777.6 0 78 232 24 777.6 0 24 233 23 777.6 0 23 234 30 777.6 0 30 235 83 777.6 0 83 236 77 777.6 0 77 237 82 777.6 0 82 238 417 777.6 0 417 239 175 777.6 0 175 240 267 777.6 0 267 241 32 777.6 0 32 242 32 777.6 0 32 243 23 777.6 0 23 244 52 777.6 0 52 245 118 777.6 0 118 246 159 777.6 0 159 247 143 777.6 0 143 248 78 777.6 0 78 249 39 777.6 0 39 250 36 777.6 0 36 251 26 777.6 0 26 252 32 777.6 0 32 253 71 777.6 0 71 254 105 777.6 0 105 255 119 777.6 0 119 256 108 777.6 0 108 257 173 777.6 0 173 258 142 777.6 0 142 259 141 777.6 0 141 260 236 777.6 0 236 261 77 777.6 0 77 262 84 777.6 0 84 263 175 777.6 0 175 264 180 777.6 0 180 265 528 777.6 0 528 266 97 777.6 0 97 267 211 777.6 0 211 268 288 777.6 0 288 269 308 777.6 0 308 270 248 777.6 0 248 271 309 777.6 0 309 272 4213 777.6 0 4213 273 8987 777.6 0 8987 274 14574 777.6 0 14574 275 152 777.6 0 152 276 55 777.6 0 55 277 55 777.6 0 55 278 273 777.6 0 273 279 153 777.6 0 153 280 151 777.6 0 151 281 69 777.6 0 69 282 73 777.6 0 73 283 96 777.6 0 96 284 277 777.6 0 277 285 537 777.6 0 537 286 264 777.6 0 264 287 162 777.6 0 162 288 95 777.6 0 95 289 83 777.6 0 83 290 101 777.6 0 101 291 90 777.6 0 90 292 84 777.6 0 84 293 223 777.6 0 223 294 182 777.6 0 182 295 268 777.6 0 268 296 210 777.6 0 210 297 223 777.6 0 223 298 265 777.6 0 265 299 683 777.6 0 683 300 2085 777.6 0 2085 301 7940 777.6 0 7940 Finished in 2.33 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 48,830 Reads with adapters: 38,165 (78.2%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 38,158 (78.1%) Total basepairs processed: 13,017,216 bp Total written (filtered): 10,568,411 bp (81.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 38165 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 8 2 16 290 0.0 3 0 234 36 20 17 37574 0.0 3 36080 1235 190 69 18 117 0.0 3 12 91 7 7 19 31 0.0 3 0 0 17 14 20 123 0.0 3 1 0 0 122 24 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 1 61 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 1 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.74 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 38,158 Reads with adapters: 1,828 (4.8%) Reads written (passing filters): 38,158 (100.0%) Total basepairs processed: 10,568,411 bp Total written (filtered): 10,562,836 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1828 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.1% C: 59.5% G: 14.5% T: 9.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1742 596.2 0 1742 4 82 149.1 0 82 5 3 37.3 0 3 6 1 9.3 0 1