This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.97 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 271,844 (8.5%) Reads that were too short: 38,093 (1.2%) Reads written (passing filters): 233,751 (7.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 44,532,714 bp (4.6%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 271844 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1146 777.6 0 1146 7 330 777.6 0 330 8 686 777.6 0 686 9 31959 777.6 0 31959 10 91 777.6 0 91 11 38 777.6 0 38 12 18 777.6 0 18 13 17 777.6 0 17 14 25 777.6 0 25 15 20 777.6 0 20 16 21 777.6 0 21 17 27 777.6 0 27 18 19 777.6 0 19 19 20 777.6 0 20 20 24 777.6 0 24 21 64 777.6 0 64 22 61 777.6 0 61 23 98 777.6 0 98 24 440 777.6 0 440 25 170 777.6 0 170 26 210 777.6 0 210 27 26 777.6 0 26 28 19 777.6 0 19 29 24 777.6 0 24 30 26 777.6 0 26 31 31 777.6 0 31 32 27 777.6 0 27 33 27 777.6 0 27 34 18 777.6 0 18 35 17 777.6 0 17 36 19 777.6 0 19 37 18 777.6 0 18 38 14 777.6 0 14 39 18 777.6 0 18 40 20 777.6 0 20 41 14 777.6 0 14 42 14 777.6 0 14 43 18 777.6 0 18 44 15 777.6 0 15 45 12 777.6 0 12 46 24 777.6 0 24 47 36 777.6 0 36 48 35 777.6 0 35 49 32 777.6 0 32 50 15 777.6 0 15 51 23 777.6 0 23 52 21 777.6 0 21 53 27 777.6 0 27 54 38 777.6 0 38 55 47 777.6 0 47 56 40 777.6 0 40 57 28 777.6 0 28 58 26 777.6 0 26 59 79 777.6 0 79 60 19 777.6 0 19 61 189 777.6 0 189 62 323 777.6 0 323 63 393 777.6 0 393 64 31 777.6 0 31 65 67 777.6 0 67 66 35 777.6 0 35 67 22 777.6 0 22 68 16 777.6 0 16 69 101 777.6 0 101 70 116 777.6 0 116 71 396 777.6 0 396 72 49 777.6 0 49 73 134 777.6 0 134 74 130 777.6 0 130 75 64 777.6 0 64 76 97 777.6 0 97 77 217 777.6 0 217 78 427 777.6 0 427 79 187 777.6 0 187 80 364 777.6 0 364 81 313 777.6 0 313 82 942 777.6 0 942 83 1136 777.6 0 1136 84 1533 777.6 0 1533 85 1118 777.6 0 1118 86 278 777.6 0 278 87 246 777.6 0 246 88 275 777.6 0 275 89 237 777.6 0 237 90 274 777.6 0 274 91 273 777.6 0 273 92 244 777.6 0 244 93 370 777.6 0 370 94 541 777.6 0 541 95 268 777.6 0 268 96 254 777.6 0 254 97 499 777.6 0 499 98 660 777.6 0 660 99 1791 777.6 0 1791 100 584 777.6 0 584 101 620 777.6 0 620 102 1172 777.6 0 1172 103 773 777.6 0 773 104 469 777.6 0 469 105 1016 777.6 0 1016 106 830 777.6 0 830 107 1678 777.6 0 1678 108 522 777.6 0 522 109 1125 777.6 0 1125 110 1521 777.6 0 1521 111 1410 777.6 0 1410 112 19294 777.6 0 19294 113 12731 777.6 0 12731 114 73607 777.6 0 73607 115 505 777.6 0 505 116 184 777.6 0 184 117 212 777.6 0 212 118 639 777.6 0 639 119 736 777.6 0 736 120 1177 777.6 0 1177 121 2337 777.6 0 2337 122 1793 777.6 0 1793 123 250 777.6 0 250 124 267 777.6 0 267 125 1233 777.6 0 1233 126 653 777.6 0 653 127 403 777.6 0 403 128 249 777.6 0 249 129 170 777.6 0 170 130 192 777.6 0 192 131 297 777.6 0 297 132 299 777.6 0 299 133 210 777.6 0 210 134 262 777.6 0 262 135 216 777.6 0 216 136 297 777.6 0 297 137 286 777.6 0 286 138 288 777.6 0 288 139 297 777.6 0 297 140 220 777.6 0 220 141 297 777.6 0 297 142 416 777.6 0 416 143 286 777.6 0 286 144 458 777.6 0 458 145 372 777.6 0 372 146 371 777.6 0 371 147 578 777.6 0 578 148 1263 777.6 0 1263 149 888 777.6 0 888 150 363 777.6 0 363 151 210 777.6 0 210 152 307 777.6 0 307 153 416 777.6 0 416 154 528 777.6 0 528 155 237 777.6 0 237 156 193 777.6 0 193 157 378 777.6 0 378 158 385 777.6 0 385 159 336 777.6 0 336 160 249 777.6 0 249 161 269 777.6 0 269 162 275 777.6 0 275 163 392 777.6 0 392 164 380 777.6 0 380 165 500 777.6 0 500 166 694 777.6 0 694 167 577 777.6 0 577 168 654 777.6 0 654 169 368 777.6 0 368 170 630 777.6 0 630 171 1883 777.6 0 1883 172 1573 777.6 0 1573 173 1462 777.6 0 1462 174 939 777.6 0 939 175 245 777.6 0 245 176 253 777.6 0 253 177 276 777.6 0 276 178 491 777.6 0 491 179 594 777.6 0 594 180 542 777.6 0 542 181 399 777.6 0 399 182 386 777.6 0 386 183 2072 777.6 0 2072 184 1212 777.6 0 1212 185 1566 777.6 0 1566 186 365 777.6 0 365 187 255 777.6 0 255 188 331 777.6 0 331 189 404 777.6 0 404 190 601 777.6 0 601 191 10268 777.6 0 10268 192 5529 777.6 0 5529 193 2786 777.6 0 2786 194 643 777.6 0 643 195 395 777.6 0 395 196 361 777.6 0 361 197 344 777.6 0 344 198 518 777.6 0 518 199 1021 777.6 0 1021 200 621 777.6 0 621 201 161 777.6 0 161 202 179 777.6 0 179 203 342 777.6 0 342 204 417 777.6 0 417 205 212 777.6 0 212 206 217 777.6 0 217 207 220 777.6 0 220 208 166 777.6 0 166 209 505 777.6 0 505 210 301 777.6 0 301 211 365 777.6 0 365 212 285 777.6 0 285 213 263 777.6 0 263 214 266 777.6 0 266 215 459 777.6 0 459 216 310 777.6 0 310 217 362 777.6 0 362 218 462 777.6 0 462 219 333 777.6 0 333 220 390 777.6 0 390 221 136 777.6 0 136 222 177 777.6 0 177 223 461 777.6 0 461 224 380 777.6 0 380 225 300 777.6 0 300 226 305 777.6 0 305 227 319 777.6 0 319 228 613 777.6 0 613 229 306 777.6 0 306 230 545 777.6 0 545 231 386 777.6 0 386 232 306 777.6 0 306 233 179 777.6 0 179 234 321 777.6 0 321 235 650 777.6 0 650 236 338 777.6 0 338 237 435 777.6 0 435 238 213 777.6 0 213 239 230 777.6 0 230 240 256 777.6 0 256 241 296 777.6 0 296 242 208 777.6 0 208 243 621 777.6 0 621 244 637 777.6 0 637 245 340 777.6 0 340 246 355 777.6 0 355 247 467 777.6 0 467 248 184 777.6 0 184 249 159 777.6 0 159 250 237 777.6 0 237 251 850 777.6 0 850 252 910 777.6 0 910 253 693 777.6 0 693 254 408 777.6 0 408 255 451 777.6 0 451 256 318 777.6 0 318 257 347 777.6 0 347 258 192 777.6 0 192 259 172 777.6 0 172 260 115 777.6 0 115 261 216 777.6 0 216 262 316 777.6 0 316 263 398 777.6 0 398 264 309 777.6 0 309 265 475 777.6 0 475 266 261 777.6 0 261 267 459 777.6 0 459 268 736 777.6 0 736 269 572 777.6 0 572 270 335 777.6 0 335 271 302 777.6 0 302 272 276 777.6 0 276 273 327 777.6 0 327 274 249 777.6 0 249 275 225 777.6 0 225 276 222 777.6 0 222 277 340 777.6 0 340 278 456 777.6 0 456 279 522 777.6 0 522 280 526 777.6 0 526 281 199 777.6 0 199 282 412 777.6 0 412 283 443 777.6 0 443 284 424 777.6 0 424 285 316 777.6 0 316 286 305 777.6 0 305 287 202 777.6 0 202 288 175 777.6 0 175 289 397 777.6 0 397 290 515 777.6 0 515 291 481 777.6 0 481 292 490 777.6 0 490 293 729 777.6 0 729 294 391 777.6 0 391 295 315 777.6 0 315 296 1005 777.6 0 1005 297 2044 777.6 0 2044 298 369 777.6 0 369 299 236 777.6 0 236 300 193 777.6 0 193 301 60 777.6 0 60 Finished in 9.58 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 233,751 Reads with adapters: 34,351 (14.7%) Reads that were too short: 334 (0.1%) Reads written (passing filters): 34,017 (14.6%) Total basepairs processed: 44,532,714 bp Total written (filtered): 9,321,096 bp (20.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34351 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 12 0.0 3 0 0 11 1 16 227 0.0 3 0 207 15 5 17 32361 0.0 3 31279 924 121 37 18 159 0.0 3 15 103 7 34 19 28 0.0 3 2 2 14 10 20 996 0.0 3 865 27 2 102 21 2 0.0 3 1 1 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 47 0.0 3 0 0 0 47 26 1 0.0 3 0 0 0 1 27 7 0.0 3 0 0 0 7 28 1 0.0 3 1 30 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 28 0.0 3 0 0 0 28 54 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 7 0.0 3 0 0 0 7 67 2 0.0 3 0 0 0 2 68 11 0.0 3 0 0 0 11 69 4 0.0 3 0 0 0 4 71 9 0.0 3 0 0 0 9 74 7 0.0 3 0 0 0 7 76 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 79 11 0.0 3 0 0 0 11 82 11 0.0 3 0 0 0 11 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 12 0.0 3 0 0 0 12 102 25 0.0 3 0 0 0 25 103 4 0.0 3 0 0 0 4 105 7 0.0 3 0 0 0 7 106 4 0.0 3 0 0 0 4 107 1 0.0 3 0 0 0 1 108 14 0.0 3 0 0 0 14 109 1 0.0 3 0 0 0 1 110 6 0.0 3 0 0 0 6 111 9 0.0 3 0 0 1 8 112 11 0.0 3 0 0 0 11 113 3 0.0 3 0 0 1 2 114 1 0.0 3 0 0 0 1 115 4 0.0 3 0 0 0 4 117 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 4 0.0 3 0 0 1 3 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 0 1 139 4 0.0 3 0 0 0 4 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 3 0.0 3 0 0 0 3 150 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 3 0.0 3 0 0 0 3 168 5 0.0 3 0 0 0 5 170 2 0.0 3 0 0 0 2 171 3 0.0 3 0 0 0 3 173 5 0.0 3 0 0 0 5 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 196 0.0 3 0 0 4 192 182 4 0.0 3 0 0 0 4 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 232 2 0.0 3 0 0 1 1 241 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.53 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 34,017 Reads with adapters: 775 (2.3%) Reads written (passing filters): 34,017 (100.0%) Total basepairs processed: 9,321,096 bp Total written (filtered): 9,318,645 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 775 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.4% C: 5.9% G: 27.5% T: 13.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 649 531.5 0 649 4 126 132.9 0 126