This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.77 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 124,414 (3.9%) Reads that were too short: 67,043 (2.1%) Reads written (passing filters): 57,371 (1.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,551,044 bp (1.4%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 124414 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 328 777.6 0 328 7 297 777.6 0 297 8 1716 777.6 0 1716 9 31352 777.6 0 31352 10 70 777.6 0 70 11 22 777.6 0 22 12 11 777.6 0 11 13 7 777.6 0 7 14 5 777.6 0 5 15 8 777.6 0 8 16 3 777.6 0 3 17 4 777.6 0 4 18 3 777.6 0 3 19 2 777.6 0 2 20 6 777.6 0 6 21 5 777.6 0 5 22 4 777.6 0 4 23 8 777.6 0 8 24 7 777.6 0 7 25 11 777.6 0 11 26 1 777.6 0 1 27 6 777.6 0 6 28 7 777.6 0 7 29 4 777.6 0 4 30 9 777.6 0 9 31 11 777.6 0 11 32 3 777.6 0 3 33 7 777.6 0 7 34 7 777.6 0 7 35 6 777.6 0 6 36 6 777.6 0 6 37 6 777.6 0 6 38 5 777.6 0 5 39 2 777.6 0 2 40 9 777.6 0 9 41 219 777.6 0 219 42 139 777.6 0 139 43 14 777.6 0 14 44 7 777.6 0 7 45 4 777.6 0 4 46 4 777.6 0 4 47 37 777.6 0 37 48 7 777.6 0 7 49 8 777.6 0 8 50 6 777.6 0 6 51 6 777.6 0 6 52 6 777.6 0 6 53 4 777.6 0 4 54 6 777.6 0 6 55 6 777.6 0 6 56 6 777.6 0 6 57 9 777.6 0 9 58 9 777.6 0 9 59 7 777.6 0 7 60 6 777.6 0 6 61 13 777.6 0 13 62 20 777.6 0 20 63 9 777.6 0 9 64 4 777.6 0 4 65 5 777.6 0 5 66 4 777.6 0 4 67 8 777.6 0 8 68 4 777.6 0 4 69 6 777.6 0 6 70 9 777.6 0 9 71 12 777.6 0 12 72 14 777.6 0 14 73 73 777.6 0 73 74 10 777.6 0 10 75 7 777.6 0 7 76 7 777.6 0 7 77 3 777.6 0 3 78 63 777.6 0 63 79 49 777.6 0 49 80 97 777.6 0 97 81 68 777.6 0 68 82 79 777.6 0 79 83 223 777.6 0 223 84 164 777.6 0 164 85 266 777.6 0 266 86 44 777.6 0 44 87 7 777.6 0 7 88 37 777.6 0 37 89 50 777.6 0 50 90 72 777.6 0 72 91 12 777.6 0 12 92 51 777.6 0 51 93 160 777.6 0 160 94 453 777.6 0 453 95 553 777.6 0 553 96 79 777.6 0 79 97 34 777.6 0 34 98 51 777.6 0 51 99 137 777.6 0 137 100 229 777.6 0 229 101 186 777.6 0 186 102 126 777.6 0 126 103 49 777.6 0 49 104 55 777.6 0 55 105 207 777.6 0 207 106 55 777.6 0 55 107 42 777.6 0 42 108 40 777.6 0 40 109 74 777.6 0 74 110 87 777.6 0 87 111 86 777.6 0 86 112 55 777.6 0 55 113 61 777.6 0 61 114 100 777.6 0 100 115 211 777.6 0 211 116 166 777.6 0 166 117 203 777.6 0 203 118 254 777.6 0 254 119 234 777.6 0 234 120 212 777.6 0 212 121 129 777.6 0 129 122 96 777.6 0 96 123 125 777.6 0 125 124 162 777.6 0 162 125 262 777.6 0 262 126 975 777.6 0 975 127 313 777.6 0 313 128 255 777.6 0 255 129 128 777.6 0 128 130 136 777.6 0 136 131 157 777.6 0 157 132 136 777.6 0 136 133 124 777.6 0 124 134 209 777.6 0 209 135 209 777.6 0 209 136 207 777.6 0 207 137 155 777.6 0 155 138 247 777.6 0 247 139 160 777.6 0 160 140 173 777.6 0 173 141 99 777.6 0 99 142 61 777.6 0 61 143 102 777.6 0 102 144 218 777.6 0 218 145 363 777.6 0 363 146 156 777.6 0 156 147 97 777.6 0 97 148 48 777.6 0 48 149 79 777.6 0 79 150 75 777.6 0 75 151 59 777.6 0 59 152 120 777.6 0 120 153 214 777.6 0 214 154 229 777.6 0 229 155 232 777.6 0 232 156 281 777.6 0 281 157 202 777.6 0 202 158 299 777.6 0 299 159 133 777.6 0 133 160 208 777.6 0 208 161 96 777.6 0 96 162 90 777.6 0 90 163 106 777.6 0 106 164 188 777.6 0 188 165 238 777.6 0 238 166 153 777.6 0 153 167 110 777.6 0 110 168 126 777.6 0 126 169 264 777.6 0 264 170 101 777.6 0 101 171 108 777.6 0 108 172 199 777.6 0 199 173 457 777.6 0 457 174 429 777.6 0 429 175 340 777.6 0 340 176 106 777.6 0 106 177 136 777.6 0 136 178 215 777.6 0 215 179 231 777.6 0 231 180 328 777.6 0 328 181 349 777.6 0 349 182 170 777.6 0 170 183 141 777.6 0 141 184 122 777.6 0 122 185 214 777.6 0 214 186 109 777.6 0 109 187 65 777.6 0 65 188 66 777.6 0 66 189 206 777.6 0 206 190 436 777.6 0 436 191 534 777.6 0 534 192 211 777.6 0 211 193 161 777.6 0 161 194 81 777.6 0 81 195 102 777.6 0 102 196 229 777.6 0 229 197 1530 777.6 0 1530 198 496 777.6 0 496 199 105 777.6 0 105 200 113 777.6 0 113 201 120 777.6 0 120 202 3432 777.6 0 3432 203 382 777.6 0 382 204 234 777.6 0 234 205 234 777.6 0 234 206 112 777.6 0 112 207 117 777.6 0 117 208 55 777.6 0 55 209 105 777.6 0 105 210 124 777.6 0 124 211 117 777.6 0 117 212 158 777.6 0 158 213 128 777.6 0 128 214 97 777.6 0 97 215 141 777.6 0 141 216 408 777.6 0 408 217 495 777.6 0 495 218 425 777.6 0 425 219 265 777.6 0 265 220 77 777.6 0 77 221 103 777.6 0 103 222 128 777.6 0 128 223 239 777.6 0 239 224 297 777.6 0 297 225 210 777.6 0 210 226 254 777.6 0 254 227 381 777.6 0 381 228 457 777.6 0 457 229 3805 777.6 0 3805 230 6902 777.6 0 6902 231 1614 777.6 0 1614 232 65 777.6 0 65 233 100 777.6 0 100 234 166 777.6 0 166 235 220 777.6 0 220 236 105 777.6 0 105 237 88 777.6 0 88 238 80 777.6 0 80 239 66 777.6 0 66 240 43 777.6 0 43 241 57 777.6 0 57 242 78 777.6 0 78 243 104 777.6 0 104 244 203 777.6 0 203 245 283 777.6 0 283 246 229 777.6 0 229 247 149 777.6 0 149 248 40 777.6 0 40 249 29 777.6 0 29 250 60 777.6 0 60 251 200 777.6 0 200 252 256 777.6 0 256 253 202 777.6 0 202 254 200 777.6 0 200 255 256 777.6 0 256 256 229 777.6 0 229 257 118 777.6 0 118 258 66 777.6 0 66 259 185 777.6 0 185 260 2079 777.6 0 2079 261 517 777.6 0 517 262 202 777.6 0 202 263 218 777.6 0 218 264 193 777.6 0 193 265 185 777.6 0 185 266 337 777.6 0 337 267 176 777.6 0 176 268 214 777.6 0 214 269 151 777.6 0 151 270 107 777.6 0 107 271 305 777.6 0 305 272 155 777.6 0 155 273 206 777.6 0 206 274 186 777.6 0 186 275 128 777.6 0 128 276 164 777.6 0 164 277 171 777.6 0 171 278 302 777.6 0 302 279 430 777.6 0 430 280 220 777.6 0 220 281 105 777.6 0 105 282 121 777.6 0 121 283 243 777.6 0 243 284 407 777.6 0 407 285 383 777.6 0 383 286 435 777.6 0 435 287 497 777.6 0 497 288 488 777.6 0 488 289 197 777.6 0 197 290 249 777.6 0 249 291 289 777.6 0 289 292 837 777.6 0 837 293 15112 777.6 0 15112 294 7151 777.6 0 7151 295 7741 777.6 0 7741 296 87 777.6 0 87 297 29 777.6 0 29 298 60 777.6 0 60 299 116 777.6 0 116 300 186 777.6 0 186 301 291 777.6 0 291 Finished in 2.77 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 57,371 Reads with adapters: 33,665 (58.7%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 33,659 (58.7%) Total basepairs processed: 13,551,044 bp Total written (filtered): 9,158,293 bp (67.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33665 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 6 0.0 3 0 0 0 0 6 17 4 0.0 3 0 0 0 0 4 18 13 0.0 3 0 0 13 19 329 0.0 3 0 311 8 3 7 20 33191 0.0 4 32588 580 19 3 1 21 95 0.0 4 22 67 5 0 1 22 9 0.0 4 0 2 5 1 1 23 9 0.0 4 0 0 0 8 1 24 3 0.0 4 1 0 0 0 2 37 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.40 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 33,659 Reads with adapters: 422 (1.3%) Reads written (passing filters): 33,659 (100.0%) Total basepairs processed: 9,158,293 bp Total written (filtered): 9,152,268 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 422 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 1.2% G: 94.8% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 525.9 0 2 4 6 131.5 0 6 6 1 8.2 0 1 8 1 0.5 0 1 9 12 0.1 0 0 12 10 246 0.0 1 4 242 11 145 0.0 1 0 145 150 1 0.0 1 0 1 208 6 0.0 1 5 1 210 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.