This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 100.89 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 87,552 (2.7%) Reads that were too short: 19,673 (0.6%) Reads written (passing filters): 67,879 (2.1%) Total basepairs processed: 958,672,960 bp Total written (filtered): 18,743,339 bp (2.0%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 87552 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 399 777.6 0 399 7 947 777.6 0 947 8 56962 777.6 0 56962 9 87 777.6 0 87 10 22 777.6 0 22 11 18 777.6 0 18 12 14 777.6 0 14 13 16 777.6 0 16 14 19 777.6 0 19 15 28 777.6 0 28 16 24 777.6 0 24 17 22 777.6 0 22 18 24 777.6 0 24 19 20 777.6 0 20 20 37 777.6 0 37 21 43 777.6 0 43 22 24 777.6 0 24 23 15 777.6 0 15 24 28 777.6 0 28 25 17 777.6 0 17 26 19 777.6 0 19 27 16 777.6 0 16 28 22 777.6 0 22 29 29 777.6 0 29 30 31 777.6 0 31 31 15 777.6 0 15 32 31 777.6 0 31 33 10 777.6 0 10 34 20 777.6 0 20 35 17 777.6 0 17 36 26 777.6 0 26 37 15 777.6 0 15 38 15 777.6 0 15 39 17 777.6 0 17 40 17 777.6 0 17 41 23 777.6 0 23 42 17 777.6 0 17 43 18 777.6 0 18 44 26 777.6 0 26 45 58 777.6 0 58 46 33 777.6 0 33 47 31 777.6 0 31 48 38 777.6 0 38 49 29 777.6 0 29 50 27 777.6 0 27 51 22 777.6 0 22 52 26 777.6 0 26 53 30 777.6 0 30 54 31 777.6 0 31 55 27 777.6 0 27 56 23 777.6 0 23 57 20 777.6 0 20 58 23 777.6 0 23 59 139 777.6 0 139 60 137 777.6 0 137 61 293 777.6 0 293 62 18 777.6 0 18 63 18 777.6 0 18 64 19 777.6 0 19 65 12 777.6 0 12 66 19 777.6 0 19 67 13 777.6 0 13 68 15 777.6 0 15 69 16 777.6 0 16 70 25 777.6 0 25 71 31 777.6 0 31 72 37 777.6 0 37 73 27 777.6 0 27 74 30 777.6 0 30 75 13 777.6 0 13 76 38 777.6 0 38 77 62 777.6 0 62 78 215 777.6 0 215 79 26 777.6 0 26 80 32 777.6 0 32 81 24 777.6 0 24 82 38 777.6 0 38 83 33 777.6 0 33 84 36 777.6 0 36 85 51 777.6 0 51 86 22 777.6 0 22 87 33 777.6 0 33 88 27 777.6 0 27 89 22 777.6 0 22 90 28 777.6 0 28 91 24 777.6 0 24 92 25 777.6 0 25 93 36 777.6 0 36 94 52 777.6 0 52 95 42 777.6 0 42 96 29 777.6 0 29 97 101 777.6 0 101 98 151 777.6 0 151 99 90 777.6 0 90 100 28 777.6 0 28 101 19 777.6 0 19 102 17 777.6 0 17 103 13 777.6 0 13 104 17 777.6 0 17 105 21 777.6 0 21 106 23 777.6 0 23 107 23 777.6 0 23 108 21 777.6 0 21 109 22 777.6 0 22 110 27 777.6 0 27 111 41 777.6 0 41 112 99 777.6 0 99 113 34 777.6 0 34 114 45 777.6 0 45 115 27 777.6 0 27 116 36 777.6 0 36 117 35 777.6 0 35 118 30 777.6 0 30 119 25 777.6 0 25 120 27 777.6 0 27 121 22 777.6 0 22 122 20 777.6 0 20 123 25 777.6 0 25 124 25 777.6 0 25 125 28 777.6 0 28 126 32 777.6 0 32 127 46 777.6 0 46 128 39 777.6 0 39 129 66 777.6 0 66 130 93 777.6 0 93 131 40 777.6 0 40 132 29 777.6 0 29 133 51 777.6 0 51 134 60 777.6 0 60 135 54 777.6 0 54 136 39 777.6 0 39 137 45 777.6 0 45 138 126 777.6 0 126 139 68 777.6 0 68 140 395 777.6 0 395 141 49 777.6 0 49 142 47 777.6 0 47 143 36 777.6 0 36 144 46 777.6 0 46 145 68 777.6 0 68 146 98 777.6 0 98 147 43 777.6 0 43 148 23 777.6 0 23 149 37 777.6 0 37 150 28 777.6 0 28 151 35 777.6 0 35 152 48 777.6 0 48 153 55 777.6 0 55 154 27 777.6 0 27 155 37 777.6 0 37 156 52 777.6 0 52 157 44 777.6 0 44 158 42 777.6 0 42 159 197 777.6 0 197 160 62 777.6 0 62 161 38 777.6 0 38 162 24 777.6 0 24 163 23 777.6 0 23 164 38 777.6 0 38 165 24 777.6 0 24 166 33 777.6 0 33 167 36 777.6 0 36 168 34 777.6 0 34 169 28 777.6 0 28 170 34 777.6 0 34 171 72 777.6 0 72 172 32 777.6 0 32 173 25 777.6 0 25 174 23 777.6 0 23 175 18 777.6 0 18 176 23 777.6 0 23 177 25 777.6 0 25 178 21 777.6 0 21 179 20 777.6 0 20 180 36 777.6 0 36 181 24 777.6 0 24 182 34 777.6 0 34 183 39 777.6 0 39 184 25 777.6 0 25 185 38 777.6 0 38 186 27 777.6 0 27 187 29 777.6 0 29 188 37 777.6 0 37 189 42 777.6 0 42 190 29 777.6 0 29 191 51 777.6 0 51 192 133 777.6 0 133 193 148 777.6 0 148 194 60 777.6 0 60 195 86 777.6 0 86 196 75 777.6 0 75 197 1217 777.6 0 1217 198 145 777.6 0 145 199 284 777.6 0 284 200 36 777.6 0 36 201 39 777.6 0 39 202 22 777.6 0 22 203 17 777.6 0 17 204 29 777.6 0 29 205 38 777.6 0 38 206 43 777.6 0 43 207 47 777.6 0 47 208 25 777.6 0 25 209 17 777.6 0 17 210 34 777.6 0 34 211 34 777.6 0 34 212 53 777.6 0 53 213 46 777.6 0 46 214 54 777.6 0 54 215 82 777.6 0 82 216 50 777.6 0 50 217 35 777.6 0 35 218 37 777.6 0 37 219 32 777.6 0 32 220 70 777.6 0 70 221 75 777.6 0 75 222 31 777.6 0 31 223 148 777.6 0 148 224 128 777.6 0 128 225 153 777.6 0 153 226 53 777.6 0 53 227 43 777.6 0 43 228 29 777.6 0 29 229 38 777.6 0 38 230 32 777.6 0 32 231 185 777.6 0 185 232 246 777.6 0 246 233 285 777.6 0 285 234 37 777.6 0 37 235 26 777.6 0 26 236 47 777.6 0 47 237 88 777.6 0 88 238 100 777.6 0 100 239 48 777.6 0 48 240 69 777.6 0 69 241 51 777.6 0 51 242 23 777.6 0 23 243 23 777.6 0 23 244 25 777.6 0 25 245 49 777.6 0 49 246 78 777.6 0 78 247 104 777.6 0 104 248 43 777.6 0 43 249 50 777.6 0 50 250 54 777.6 0 54 251 46 777.6 0 46 252 33 777.6 0 33 253 35 777.6 0 35 254 45 777.6 0 45 255 42 777.6 0 42 256 46 777.6 0 46 257 61 777.6 0 61 258 186 777.6 0 186 259 33 777.6 0 33 260 42 777.6 0 42 261 52 777.6 0 52 262 103 777.6 0 103 263 48 777.6 0 48 264 41 777.6 0 41 265 106 777.6 0 106 266 434 777.6 0 434 267 363 777.6 0 363 268 140 777.6 0 140 269 54 777.6 0 54 270 42 777.6 0 42 271 44 777.6 0 44 272 55 777.6 0 55 273 39 777.6 0 39 274 55 777.6 0 55 275 55 777.6 0 55 276 39 777.6 0 39 277 41 777.6 0 41 278 67 777.6 0 67 279 68 777.6 0 68 280 85 777.6 0 85 281 130 777.6 0 130 282 116 777.6 0 116 283 123 777.6 0 123 284 131 777.6 0 131 285 233 777.6 0 233 286 204 777.6 0 204 287 137 777.6 0 137 288 184 777.6 0 184 289 311 777.6 0 311 290 526 777.6 0 526 291 450 777.6 0 450 292 402 777.6 0 402 293 415 777.6 0 415 294 376 777.6 0 376 295 301 777.6 0 301 296 332 777.6 0 332 297 626 777.6 0 626 298 2133 777.6 0 2133 299 2481 777.6 0 2481 300 1792 777.6 0 1792 301 2644 777.6 0 2644 Finished in 3.26 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 67,879 Reads with adapters: 58,056 (85.5%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 58,047 (85.5%) Total basepairs processed: 18,743,339 bp Total written (filtered): 16,020,266 bp (85.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58056 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 19 0.0 3 0 0 14 5 16 446 0.0 3 0 383 46 17 17 56863 0.0 3 55324 1377 134 28 18 257 0.0 3 9 138 14 96 19 116 0.0 3 0 0 89 27 20 330 0.0 3 0 0 0 330 22 1 0.0 3 1 24 2 0.0 3 2 29 2 0.0 3 1 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.59 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 58,047 Reads with adapters: 574 (1.0%) Reads written (passing filters): 58,047 (100.0%) Total basepairs processed: 16,020,266 bp Total written (filtered): 16,018,444 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 574 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.5% C: 14.1% G: 25.8% T: 22.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 492 907.0 0 492 4 66 226.7 0 66 5 14 56.7 0 14 6 2 14.2 0 2