This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.99 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 39,652 (1.2%) Reads that were too short: 19,314 (0.6%) Reads written (passing filters): 20,338 (0.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 3,991,083 bp (0.4%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 39652 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 88 777.6 0 88 7 2206 777.6 0 2206 8 42 777.6 0 42 9 20 777.6 0 20 10 23 777.6 0 23 11 19 777.6 0 19 12 13 777.6 0 13 13 19 777.6 0 19 14 21 777.6 0 21 15 19 777.6 0 19 16 19 777.6 0 19 17 18 777.6 0 18 18 18 777.6 0 18 19 23 777.6 0 23 20 14 777.6 0 14 21 21 777.6 0 21 22 30 777.6 0 30 23 21 777.6 0 21 24 15 777.6 0 15 25 28 777.6 0 28 26 12 777.6 0 12 27 23 777.6 0 23 28 21 777.6 0 21 29 20 777.6 0 20 30 25 777.6 0 25 31 18 777.6 0 18 32 15 777.6 0 15 33 22 777.6 0 22 34 116 777.6 0 116 35 29 777.6 0 29 36 22 777.6 0 22 37 29 777.6 0 29 38 24 777.6 0 24 39 23 777.6 0 23 40 16 777.6 0 16 41 26 777.6 0 26 42 22 777.6 0 22 43 38 777.6 0 38 44 21 777.6 0 21 45 17 777.6 0 17 46 20 777.6 0 20 47 35 777.6 0 35 48 19 777.6 0 19 49 21 777.6 0 21 50 21 777.6 0 21 51 24 777.6 0 24 52 34 777.6 0 34 53 83 777.6 0 83 54 45 777.6 0 45 55 29 777.6 0 29 56 21 777.6 0 21 57 20 777.6 0 20 58 26 777.6 0 26 59 37 777.6 0 37 60 41 777.6 0 41 61 31 777.6 0 31 62 62 777.6 0 62 63 87 777.6 0 87 64 2602 777.6 0 2602 65 1064 777.6 0 1064 66 945 777.6 0 945 67 18 777.6 0 18 68 17 777.6 0 17 69 25 777.6 0 25 70 35 777.6 0 35 71 20 777.6 0 20 72 40 777.6 0 40 73 13 777.6 0 13 74 13 777.6 0 13 75 22 777.6 0 22 76 18 777.6 0 18 77 16 777.6 0 16 78 9 777.6 0 9 79 34 777.6 0 34 80 27 777.6 0 27 81 42 777.6 0 42 82 25 777.6 0 25 83 79 777.6 0 79 84 59 777.6 0 59 85 63 777.6 0 63 86 51 777.6 0 51 87 45 777.6 0 45 88 25 777.6 0 25 89 214 777.6 0 214 90 81 777.6 0 81 91 68 777.6 0 68 92 173 777.6 0 173 93 77 777.6 0 77 94 118 777.6 0 118 95 126 777.6 0 126 96 89 777.6 0 89 97 34 777.6 0 34 98 16 777.6 0 16 99 46 777.6 0 46 100 68 777.6 0 68 101 164 777.6 0 164 102 28 777.6 0 28 103 41 777.6 0 41 104 26 777.6 0 26 105 21 777.6 0 21 106 33 777.6 0 33 107 30 777.6 0 30 108 18 777.6 0 18 109 18 777.6 0 18 110 23 777.6 0 23 111 36 777.6 0 36 112 23 777.6 0 23 113 24 777.6 0 24 114 39 777.6 0 39 115 37 777.6 0 37 116 55 777.6 0 55 117 37 777.6 0 37 118 58 777.6 0 58 119 53 777.6 0 53 120 28 777.6 0 28 121 114 777.6 0 114 122 68 777.6 0 68 123 53 777.6 0 53 124 100 777.6 0 100 125 45 777.6 0 45 126 46 777.6 0 46 127 48 777.6 0 48 128 75 777.6 0 75 129 52 777.6 0 52 130 81 777.6 0 81 131 83 777.6 0 83 132 112 777.6 0 112 133 67 777.6 0 67 134 44 777.6 0 44 135 99 777.6 0 99 136 159 777.6 0 159 137 246 777.6 0 246 138 95 777.6 0 95 139 73 777.6 0 73 140 50 777.6 0 50 141 69 777.6 0 69 142 68 777.6 0 68 143 60 777.6 0 60 144 30 777.6 0 30 145 39 777.6 0 39 146 69 777.6 0 69 147 148 777.6 0 148 148 163 777.6 0 163 149 117 777.6 0 117 150 96 777.6 0 96 151 68 777.6 0 68 152 74 777.6 0 74 153 103 777.6 0 103 154 70 777.6 0 70 155 119 777.6 0 119 156 139 777.6 0 139 157 366 777.6 0 366 158 272 777.6 0 272 159 154 777.6 0 154 160 138 777.6 0 138 161 104 777.6 0 104 162 138 777.6 0 138 163 149 777.6 0 149 164 120 777.6 0 120 165 65 777.6 0 65 166 109 777.6 0 109 167 148 777.6 0 148 168 194 777.6 0 194 169 136 777.6 0 136 170 175 777.6 0 175 171 457 777.6 0 457 172 104 777.6 0 104 173 108 777.6 0 108 174 71 777.6 0 71 175 379 777.6 0 379 176 291 777.6 0 291 177 152 777.6 0 152 178 88 777.6 0 88 179 35 777.6 0 35 180 72 777.6 0 72 181 119 777.6 0 119 182 127 777.6 0 127 183 130 777.6 0 130 184 140 777.6 0 140 185 89 777.6 0 89 186 186 777.6 0 186 187 255 777.6 0 255 188 234 777.6 0 234 189 65 777.6 0 65 190 63 777.6 0 63 191 45 777.6 0 45 192 57 777.6 0 57 193 87 777.6 0 87 194 88 777.6 0 88 195 70 777.6 0 70 196 44 777.6 0 44 197 46 777.6 0 46 198 36 777.6 0 36 199 83 777.6 0 83 200 87 777.6 0 87 201 39 777.6 0 39 202 53 777.6 0 53 203 44 777.6 0 44 204 93 777.6 0 93 205 100 777.6 0 100 206 109 777.6 0 109 207 111 777.6 0 111 208 47 777.6 0 47 209 81 777.6 0 81 210 78 777.6 0 78 211 76 777.6 0 76 212 91 777.6 0 91 213 98 777.6 0 98 214 125 777.6 0 125 215 157 777.6 0 157 216 164 777.6 0 164 217 147 777.6 0 147 218 214 777.6 0 214 219 116 777.6 0 116 220 143 777.6 0 143 221 136 777.6 0 136 222 277 777.6 0 277 223 822 777.6 0 822 224 234 777.6 0 234 225 197 777.6 0 197 226 208 777.6 0 208 227 181 777.6 0 181 228 174 777.6 0 174 229 136 777.6 0 136 230 108 777.6 0 108 231 59 777.6 0 59 232 70 777.6 0 70 233 60 777.6 0 60 234 68 777.6 0 68 235 76 777.6 0 76 236 84 777.6 0 84 237 110 777.6 0 110 238 71 777.6 0 71 239 70 777.6 0 70 240 41 777.6 0 41 241 110 777.6 0 110 242 176 777.6 0 176 243 383 777.6 0 383 244 262 777.6 0 262 245 236 777.6 0 236 246 138 777.6 0 138 247 212 777.6 0 212 248 201 777.6 0 201 249 181 777.6 0 181 250 103 777.6 0 103 251 486 777.6 0 486 252 901 777.6 0 901 253 492 777.6 0 492 254 506 777.6 0 506 255 430 777.6 0 430 256 249 777.6 0 249 257 261 777.6 0 261 258 254 777.6 0 254 259 72 777.6 0 72 260 54 777.6 0 54 261 50 777.6 0 50 262 50 777.6 0 50 263 58 777.6 0 58 264 49 777.6 0 49 265 91 777.6 0 91 266 125 777.6 0 125 267 321 777.6 0 321 268 977 777.6 0 977 269 540 777.6 0 540 270 1624 777.6 0 1624 271 73 777.6 0 73 272 69 777.6 0 69 273 168 777.6 0 168 274 103 777.6 0 103 275 109 777.6 0 109 276 67 777.6 0 67 277 45 777.6 0 45 278 49 777.6 0 49 279 125 777.6 0 125 280 227 777.6 0 227 281 155 777.6 0 155 282 188 777.6 0 188 283 131 777.6 0 131 284 92 777.6 0 92 285 76 777.6 0 76 286 72 777.6 0 72 287 141 777.6 0 141 288 238 777.6 0 238 289 201 777.6 0 201 290 61 777.6 0 61 291 51 777.6 0 51 292 138 777.6 0 138 293 170 777.6 0 170 294 268 777.6 0 268 295 114 777.6 0 114 296 76 777.6 0 76 297 43 777.6 0 43 298 81 777.6 0 81 299 96 777.6 0 96 300 674 777.6 0 674 301 392 777.6 0 392 Finished in 0.91 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 20,338 Reads with adapters: 2,398 (11.8%) Reads that were too short: 123 (0.6%) Reads written (passing filters): 2,275 (11.2%) Total basepairs processed: 3,991,083 bp Total written (filtered): 624,270 bp (15.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2398 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 13 0.0 3 0 11 1 1 17 1737 0.0 3 1676 50 10 1 18 12 0.0 3 3 6 2 1 19 474 0.0 3 458 12 3 1 20 16 0.0 3 3 0 0 13 21 1 0.0 3 0 0 1 22 2 0.0 3 0 0 0 2 23 5 0.0 3 0 0 0 5 27 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 36 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 71 1 0.0 3 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 110 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 110 0.0 3 0 1 8 101 175 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,275 Reads with adapters: 171 (7.5%) Reads written (passing filters): 2,275 (100.0%) Total basepairs processed: 624,270 bp Total written (filtered): 623,751 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 171 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.3% C: 27.5% G: 17.5% T: 28.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 165 35.5 0 165 4 6 8.9 0 6