This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.34 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 327,309 (10.3%) Reads that were too short: 252,825 (7.9%) Reads written (passing filters): 74,484 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 12,909,939 bp (1.3%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 327309 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 23 777.6 0 23 7 46 777.6 0 46 8 79 777.6 0 79 9 2577 777.6 0 2577 10 15 777.6 0 15 11 28 777.6 0 28 12 13 777.6 0 13 13 8 777.6 0 8 14 15 777.6 0 15 15 18 777.6 0 18 16 17 777.6 0 17 17 31 777.6 0 31 18 15 777.6 0 15 19 13 777.6 0 13 20 17 777.6 0 17 21 22 777.6 0 22 22 17 777.6 0 17 23 13 777.6 0 13 24 17 777.6 0 17 25 23 777.6 0 23 26 20 777.6 0 20 27 21 777.6 0 21 28 28 777.6 0 28 29 27 777.6 0 27 30 34 777.6 0 34 31 20 777.6 0 20 32 24 777.6 0 24 33 19 777.6 0 19 34 24 777.6 0 24 35 39 777.6 0 39 36 186 777.6 0 186 37 42 777.6 0 42 38 149 777.6 0 149 39 144 777.6 0 144 40 132 777.6 0 132 41 30 777.6 0 30 42 114 777.6 0 114 43 87 777.6 0 87 44 89 777.6 0 89 45 39 777.6 0 39 46 20 777.6 0 20 47 40 777.6 0 40 48 56 777.6 0 56 49 105 777.6 0 105 50 45 777.6 0 45 51 151 777.6 0 151 52 50 777.6 0 50 53 40 777.6 0 40 54 46 777.6 0 46 55 46 777.6 0 46 56 636 777.6 0 636 57 109 777.6 0 109 58 28 777.6 0 28 59 17 777.6 0 17 60 17 777.6 0 17 61 18 777.6 0 18 62 49 777.6 0 49 63 33 777.6 0 33 64 44 777.6 0 44 65 46 777.6 0 46 66 34 777.6 0 34 67 75 777.6 0 75 68 37 777.6 0 37 69 188 777.6 0 188 70 188 777.6 0 188 71 124 777.6 0 124 72 111 777.6 0 111 73 176 777.6 0 176 74 136 777.6 0 136 75 722 777.6 0 722 76 226 777.6 0 226 77 772 777.6 0 772 78 2159 777.6 0 2159 79 453 777.6 0 453 80 402 777.6 0 402 81 580 777.6 0 580 82 404 777.6 0 404 83 218 777.6 0 218 84 342 777.6 0 342 85 130 777.6 0 130 86 208 777.6 0 208 87 308 777.6 0 308 88 523 777.6 0 523 89 69 777.6 0 69 90 319 777.6 0 319 91 530 777.6 0 530 92 289 777.6 0 289 93 407 777.6 0 407 94 290 777.6 0 290 95 197 777.6 0 197 96 229 777.6 0 229 97 497 777.6 0 497 98 3634 777.6 0 3634 99 1038 777.6 0 1038 100 173 777.6 0 173 101 499 777.6 0 499 102 132 777.6 0 132 103 146 777.6 0 146 104 381 777.6 0 381 105 156 777.6 0 156 106 76 777.6 0 76 107 571 777.6 0 571 108 972 777.6 0 972 109 159 777.6 0 159 110 418 777.6 0 418 111 787 777.6 0 787 112 586 777.6 0 586 113 358 777.6 0 358 114 342 777.6 0 342 115 386 777.6 0 386 116 496 777.6 0 496 117 419 777.6 0 419 118 151 777.6 0 151 119 703 777.6 0 703 120 386 777.6 0 386 121 376 777.6 0 376 122 214 777.6 0 214 123 272 777.6 0 272 124 377 777.6 0 377 125 623 777.6 0 623 126 246 777.6 0 246 127 143 777.6 0 143 128 364 777.6 0 364 129 651 777.6 0 651 130 556 777.6 0 556 131 310 777.6 0 310 132 545 777.6 0 545 133 541 777.6 0 541 134 729 777.6 0 729 135 280 777.6 0 280 136 276 777.6 0 276 137 274 777.6 0 274 138 345 777.6 0 345 139 1769 777.6 0 1769 140 506 777.6 0 506 141 438 777.6 0 438 142 202 777.6 0 202 143 324 777.6 0 324 144 244 777.6 0 244 145 358 777.6 0 358 146 233 777.6 0 233 147 249 777.6 0 249 148 243 777.6 0 243 149 244 777.6 0 244 150 305 777.6 0 305 151 187 777.6 0 187 152 264 777.6 0 264 153 235 777.6 0 235 154 349 777.6 0 349 155 663 777.6 0 663 156 343 777.6 0 343 157 480 777.6 0 480 158 558 777.6 0 558 159 10303 777.6 0 10303 160 3542 777.6 0 3542 161 4055 777.6 0 4055 162 182 777.6 0 182 163 248 777.6 0 248 164 244 777.6 0 244 165 326 777.6 0 326 166 269 777.6 0 269 167 303 777.6 0 303 168 239 777.6 0 239 169 112 777.6 0 112 170 105 777.6 0 105 171 177 777.6 0 177 172 279 777.6 0 279 173 128 777.6 0 128 174 170 777.6 0 170 175 248 777.6 0 248 176 201 777.6 0 201 177 220 777.6 0 220 178 307 777.6 0 307 179 202 777.6 0 202 180 339 777.6 0 339 181 307 777.6 0 307 182 221 777.6 0 221 183 254 777.6 0 254 184 244 777.6 0 244 185 274 777.6 0 274 186 161 777.6 0 161 187 183 777.6 0 183 188 296 777.6 0 296 189 295 777.6 0 295 190 350 777.6 0 350 191 290 777.6 0 290 192 391 777.6 0 391 193 286 777.6 0 286 194 298 777.6 0 298 195 488 777.6 0 488 196 716 777.6 0 716 197 234 777.6 0 234 198 103 777.6 0 103 199 136 777.6 0 136 200 418 777.6 0 418 201 841 777.6 0 841 202 675 777.6 0 675 203 378 777.6 0 378 204 528 777.6 0 528 205 345 777.6 0 345 206 415 777.6 0 415 207 851 777.6 0 851 208 1246 777.6 0 1246 209 1288 777.6 0 1288 210 480 777.6 0 480 211 595 777.6 0 595 212 672 777.6 0 672 213 1298 777.6 0 1298 214 723 777.6 0 723 215 377 777.6 0 377 216 274 777.6 0 274 217 202 777.6 0 202 218 352 777.6 0 352 219 282 777.6 0 282 220 399 777.6 0 399 221 1018 777.6 0 1018 222 488 777.6 0 488 223 525 777.6 0 525 224 2945 777.6 0 2945 225 293 777.6 0 293 226 214 777.6 0 214 227 387 777.6 0 387 228 603 777.6 0 603 229 379 777.6 0 379 230 342 777.6 0 342 231 492 777.6 0 492 232 2466 777.6 0 2466 233 4443 777.6 0 4443 234 2096 777.6 0 2096 235 531 777.6 0 531 236 426 777.6 0 426 237 475 777.6 0 475 238 714 777.6 0 714 239 419 777.6 0 419 240 725 777.6 0 725 241 740 777.6 0 740 242 1012 777.6 0 1012 243 640 777.6 0 640 244 416 777.6 0 416 245 375 777.6 0 375 246 149 777.6 0 149 247 247 777.6 0 247 248 461 777.6 0 461 249 835 777.6 0 835 250 1625 777.6 0 1625 251 1459 777.6 0 1459 252 1614 777.6 0 1614 253 2684 777.6 0 2684 254 9855 777.6 0 9855 255 69764 777.6 0 69764 256 59625 777.6 0 59625 257 42479 777.6 0 42479 258 1042 777.6 0 1042 259 405 777.6 0 405 260 543 777.6 0 543 261 1337 777.6 0 1337 262 1451 777.6 0 1451 263 2088 777.6 0 2088 264 485 777.6 0 485 265 2028 777.6 0 2028 266 443 777.6 0 443 267 169 777.6 0 169 268 212 777.6 0 212 269 262 777.6 0 262 270 301 777.6 0 301 271 522 777.6 0 522 272 366 777.6 0 366 273 311 777.6 0 311 274 261 777.6 0 261 275 237 777.6 0 237 276 264 777.6 0 264 277 233 777.6 0 233 278 239 777.6 0 239 279 190 777.6 0 190 280 187 777.6 0 187 281 239 777.6 0 239 282 351 777.6 0 351 283 544 777.6 0 544 284 415 777.6 0 415 285 253 777.6 0 253 286 323 777.6 0 323 287 306 777.6 0 306 288 233 777.6 0 233 289 514 777.6 0 514 290 472 777.6 0 472 291 484 777.6 0 484 292 282 777.6 0 282 293 235 777.6 0 235 294 238 777.6 0 238 295 392 777.6 0 392 296 423 777.6 0 423 297 1100 777.6 0 1100 298 2075 777.6 0 2075 299 1497 777.6 0 1497 300 611 777.6 0 611 301 2921 777.6 0 2921 Finished in 2.95 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 74,484 Reads with adapters: 2,637 (3.5%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 2,617 (3.5%) Total basepairs processed: 12,909,939 bp Total written (filtered): 717,441 bp (5.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2637 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 64 0.0 3 0 56 1 7 17 2516 0.0 3 2409 101 5 1 18 12 0.0 3 0 6 2 4 19 4 0.0 3 0 0 2 2 20 4 0.0 3 1 0 0 3 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 2,617 Reads with adapters: 92 (3.5%) Reads written (passing filters): 2,617 (100.0%) Total basepairs processed: 717,441 bp Total written (filtered): 717,134 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 92 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.7% C: 6.5% G: 33.7% T: 26.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 61 40.9 0 61 4 31 10.2 0 31